TY  - DATA
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani K.
AU  - Neels, Antonia
AU  - Anđelković, Boban D.
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh P.
AU  - O'Connor, Kevin E.
AU  - Witko, Tomasz
AU  - Solarz, Daria
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4439
PB  - Elsevier
T2  - Materials Letters
T1  - Supplementary data for the article: Malagurski, I.; Frison, R.; Maurya, A. K.; Neels, A.; Andjelkovic, B.; Senthamaraikannan, R.; Babu, R. P.; O’Connor, K. E.; Witko, T.; Solarz, D.; Nikodinovic-Runic, J. Polyhydroxyoctanoate Films Reinforced with Titanium Dioxide Microfibers for Biomedical Application. Materials Letters 2021, 285, 129100. https://doi.org/10.1016/j.matlet.2020.129100.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4439
ER  - 

@misc{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani K. and Neels, Antonia and Anđelković, Boban D. and Senthamaraikannan, Ramsankar and Babu, Ramesh P. and O'Connor, Kevin E. and Witko, Tomasz and Solarz, Daria and Nikodinović-Runić, Jasmina",
year = "2021",
publisher = "Elsevier",
journal = "Materials Letters",
title = "Supplementary data for the article: Malagurski, I.; Frison, R.; Maurya, A. K.; Neels, A.; Andjelkovic, B.; Senthamaraikannan, R.; Babu, R. P.; O’Connor, K. E.; Witko, T.; Solarz, D.; Nikodinovic-Runic, J. Polyhydroxyoctanoate Films Reinforced with Titanium Dioxide Microfibers for Biomedical Application. Materials Letters 2021, 285, 129100. https://doi.org/10.1016/j.matlet.2020.129100.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4439"
}

Malagurski, I., Frison, R., Maurya, A. K., Neels, A., Anđelković, B. D., Senthamaraikannan, R., Babu, R. P., O'Connor, K. E., Witko, T., Solarz, D.,& Nikodinović-Runić, J.. (2021). Supplementary data for the article: Malagurski, I.; Frison, R.; Maurya, A. K.; Neels, A.; Andjelkovic, B.; Senthamaraikannan, R.; Babu, R. P.; O’Connor, K. E.; Witko, T.; Solarz, D.; Nikodinovic-Runic, J. Polyhydroxyoctanoate Films Reinforced with Titanium Dioxide Microfibers for Biomedical Application. Materials Letters 2021, 285, 129100. https://doi.org/10.1016/j.matlet.2020.129100.. in Materials Letters
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4439

Malagurski I, Frison R, Maurya AK, Neels A, Anđelković BD, Senthamaraikannan R, Babu RP, O'Connor KE, Witko T, Solarz D, Nikodinović-Runić J. Supplementary data for the article: Malagurski, I.; Frison, R.; Maurya, A. K.; Neels, A.; Andjelkovic, B.; Senthamaraikannan, R.; Babu, R. P.; O’Connor, K. E.; Witko, T.; Solarz, D.; Nikodinovic-Runic, J. Polyhydroxyoctanoate Films Reinforced with Titanium Dioxide Microfibers for Biomedical Application. Materials Letters 2021, 285, 129100. https://doi.org/10.1016/j.matlet.2020.129100.. in Materials Letters. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4439 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani K., Neels, Antonia, Anđelković, Boban D., Senthamaraikannan, Ramsankar, Babu, Ramesh P., O'Connor, Kevin E., Witko, Tomasz, Solarz, Daria, Nikodinović-Runić, Jasmina, "Supplementary data for the article: Malagurski, I.; Frison, R.; Maurya, A. K.; Neels, A.; Andjelkovic, B.; Senthamaraikannan, R.; Babu, R. P.; O’Connor, K. E.; Witko, T.; Solarz, D.; Nikodinovic-Runic, J. Polyhydroxyoctanoate Films Reinforced with Titanium Dioxide Microfibers for Biomedical Application. Materials Letters 2021, 285, 129100. https://doi.org/10.1016/j.matlet.2020.129100." in Materials Letters (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4439 .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani K.
AU  - Neels, Antonia
AU  - Anđelković, Boban D.
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh P.
AU  - O'Connor, Kevin E.
AU  - Witko, Tomasz
AU  - Solarz, Daria
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4437
AB  - New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.
PB  - Elsevier
T2  - Materials Letters
T1  - Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application
VL  - 285
SP  - 129100
DO  - 10.1016/j.matlet.2020.129100
ER  - 

@article{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani K. and Neels, Antonia and Anđelković, Boban D. and Senthamaraikannan, Ramsankar and Babu, Ramesh P. and O'Connor, Kevin E. and Witko, Tomasz and Solarz, Daria and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.",
publisher = "Elsevier",
journal = "Materials Letters",
title = "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application",
volume = "285",
pages = "129100",
doi = "10.1016/j.matlet.2020.129100"
}

Malagurski, I., Frison, R., Maurya, A. K., Neels, A., Anđelković, B. D., Senthamaraikannan, R., Babu, R. P., O'Connor, K. E., Witko, T., Solarz, D.,& Nikodinović-Runić, J.. (2021). Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters
Elsevier., 285, 129100.
https://doi.org/10.1016/j.matlet.2020.129100

Malagurski I, Frison R, Maurya AK, Neels A, Anđelković BD, Senthamaraikannan R, Babu RP, O'Connor KE, Witko T, Solarz D, Nikodinović-Runić J. Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters. 2021;285:129100.
doi:10.1016/j.matlet.2020.129100 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani K., Neels, Antonia, Anđelković, Boban D., Senthamaraikannan, Ramsankar, Babu, Ramesh P., O'Connor, Kevin E., Witko, Tomasz, Solarz, Daria, Nikodinović-Runić, Jasmina, "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application" in Materials Letters, 285 (2021):129100,
https://doi.org/10.1016/j.matlet.2020.129100 . .

TY  - DATA
AU  - Simić, Stefan
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Lončar, Nikola L.
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh P.
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3357
T2  - Enzyme and Microbial Technology
T1  - Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3357
ER  - 

@misc{
author = "Simić, Stefan and Jeremić, Sanja and Đokić, Lidija and Božić, Nataša and Vujčić, Zoran and Lončar, Nikola L. and Senthamaraikannan, Ramsankar and Babu, Ramesh P. and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2020",
journal = "Enzyme and Microbial Technology",
title = "Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3357"
}

Simić, S., Jeremić, S., Đokić, L., Božić, N., Vujčić, Z., Lončar, N. L., Senthamaraikannan, R., Babu, R. P., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411. in Enzyme and Microbial Technology.
https://hdl.handle.net/21.15107/rcub_cherry_3357

Simić S, Jeremić S, Đokić L, Božić N, Vujčić Z, Lončar NL, Senthamaraikannan R, Babu RP, Opsenica I, Nikodinović-Runić J. Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411. in Enzyme and Microbial Technology. 2020;.
https://hdl.handle.net/21.15107/rcub_cherry_3357 .

Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Božić, Nataša, Vujčić, Zoran, Lončar, Nikola L., Senthamaraikannan, Ramsankar, Babu, Ramesh P., Opsenica, Igor, Nikodinović-Runić, Jasmina, "Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411" in Enzyme and Microbial Technology (2020),
https://hdl.handle.net/21.15107/rcub_cherry_3357 .

TY  - JOUR
AU  - Simić, Stefan
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Lončar, Nikola L.
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh P.
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3356
AB  - Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7–24 h with good yields (70–99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 °C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.
T2  - Enzyme and Microbial Technology
T1  - Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
VL  - 132
DO  - 10.1016/j.enzmictec.2019.109411
ER  - 

@article{
author = "Simić, Stefan and Jeremić, Sanja and Đokić, Lidija and Božić, Nataša and Vujčić, Zoran and Lončar, Nikola L. and Senthamaraikannan, Ramsankar and Babu, Ramesh P. and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7–24 h with good yields (70–99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 °C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.",
journal = "Enzyme and Microbial Technology",
title = "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines",
volume = "132",
doi = "10.1016/j.enzmictec.2019.109411"
}

Simić, S., Jeremić, S., Đokić, L., Božić, N., Vujčić, Z., Lončar, N. L., Senthamaraikannan, R., Babu, R. P., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology, 132.
https://doi.org/10.1016/j.enzmictec.2019.109411

Simić S, Jeremić S, Đokić L, Božić N, Vujčić Z, Lončar NL, Senthamaraikannan R, Babu RP, Opsenica I, Nikodinović-Runić J. Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology. 2020;132.
doi:10.1016/j.enzmictec.2019.109411 .

Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Božić, Nataša, Vujčić, Zoran, Lončar, Nikola L., Senthamaraikannan, Ramsankar, Babu, Ramesh P., Opsenica, Igor, Nikodinović-Runić, Jasmina, "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines" in Enzyme and Microbial Technology, 132 (2020),
https://doi.org/10.1016/j.enzmictec.2019.109411 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Ajdačić, Vladimir
AU  - Božinović, Nina S.
AU  - Pavlović, Vladimir D.
AU  - Manojlović, Dragan D.
AU  - Babu, Ramesh P.
AU  - Senthamaraikannan, Ramsankar
AU  - Rojas, Orlando
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2866
AB  - Bacterial nanocellulose (BNC) emerged as an attractive advanced biomaterial that provides desirable properties such as high strength, lightweight, tailorable surface chemistry, hydrophilicity, and biodegradability. BNC was successfully obtained from a wide range of carbon sources including sugars derived from grass biomass using Komagataeibacter medellinensis ID13488 strain with yields up to 6 g L −1 in static fermentation. Produced BNC was utilized in straightforward catalyst preparation as a solid support for two different transition metals, palladium and copper with metal loading of 20 and 3 wt%, respectively. Sustainable catalysts were applied in the synthesis of valuable fine chemicals, such as biphenyl-4-amine and 4′-fluorobiphenyl-4-amine, used in drug discovery, perfumes and dye industries with excellent product yields of up to 99%. Pd/BNC catalyst was reused 4 times and applied in two consecutive reactions, Suzuki-Miyaura cross-coupling reaction followed by hydrogenation of nitro to amino group while Cu/BNC catalyst was examined in Chan-Lam coupling reaction. Overall, the environmentally benign process of obtaining nanocellulose from biomass, followed by its utilisation as a solid support in metal-catalysed reactions and its recovery has been described. These findings reveal that BNC is a good support material, and it can be used as a support for different catalytic systems.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions
VL  - 129
SP  - 351
EP  - 360
DO  - 10.1016/j.ijbiomac.2019.01.154
ER  - 

@article{
author = "Jeremić, Sanja and Đokić, Lidija and Ajdačić, Vladimir and Božinović, Nina S. and Pavlović, Vladimir D. and Manojlović, Dragan D. and Babu, Ramesh P. and Senthamaraikannan, Ramsankar and Rojas, Orlando and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2019",
abstract = "Bacterial nanocellulose (BNC) emerged as an attractive advanced biomaterial that provides desirable properties such as high strength, lightweight, tailorable surface chemistry, hydrophilicity, and biodegradability. BNC was successfully obtained from a wide range of carbon sources including sugars derived from grass biomass using Komagataeibacter medellinensis ID13488 strain with yields up to 6 g L −1 in static fermentation. Produced BNC was utilized in straightforward catalyst preparation as a solid support for two different transition metals, palladium and copper with metal loading of 20 and 3 wt%, respectively. Sustainable catalysts were applied in the synthesis of valuable fine chemicals, such as biphenyl-4-amine and 4′-fluorobiphenyl-4-amine, used in drug discovery, perfumes and dye industries with excellent product yields of up to 99%. Pd/BNC catalyst was reused 4 times and applied in two consecutive reactions, Suzuki-Miyaura cross-coupling reaction followed by hydrogenation of nitro to amino group while Cu/BNC catalyst was examined in Chan-Lam coupling reaction. Overall, the environmentally benign process of obtaining nanocellulose from biomass, followed by its utilisation as a solid support in metal-catalysed reactions and its recovery has been described. These findings reveal that BNC is a good support material, and it can be used as a support for different catalytic systems.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions",
volume = "129",
pages = "351-360",
doi = "10.1016/j.ijbiomac.2019.01.154"
}

Jeremić, S., Đokić, L., Ajdačić, V., Božinović, N. S., Pavlović, V. D., Manojlović, D. D., Babu, R. P., Senthamaraikannan, R., Rojas, O., Opsenica, I.,& Nikodinović-Runić, J.. (2019). Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions. in International Journal of Biological Macromolecules
Elsevier., 129, 351-360.
https://doi.org/10.1016/j.ijbiomac.2019.01.154

Jeremić S, Đokić L, Ajdačić V, Božinović NS, Pavlović VD, Manojlović DD, Babu RP, Senthamaraikannan R, Rojas O, Opsenica I, Nikodinović-Runić J. Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions. in International Journal of Biological Macromolecules. 2019;129:351-360.
doi:10.1016/j.ijbiomac.2019.01.154 .

Jeremić, Sanja, Đokić, Lidija, Ajdačić, Vladimir, Božinović, Nina S., Pavlović, Vladimir D., Manojlović, Dragan D., Babu, Ramesh P., Senthamaraikannan, Ramsankar, Rojas, Orlando, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions" in International Journal of Biological Macromolecules, 129 (2019):351-360,
https://doi.org/10.1016/j.ijbiomac.2019.01.154 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Ajdačić, Vladimir
AU  - Božinović, Nina S.
AU  - Pavlović, Vladimir D.
AU  - Manojlović, Dragan D.
AU  - Babu, Ramesh P.
AU  - Senthamaraikannan, Ramsankar
AU  - Rojas, Orlando
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2850
AB  - Bacterial nanocellulose (BNC) emerged as an attractive advanced biomaterial that provides desirable properties such as high strength, lightweight, tailorable surface chemistry, hydrophilicity, and biodegradability. BNC was successfully obtained from a wide range of carbon sources including sugars derived from grass biomass using Komagataeibacter medellinensis ID13488 strain with yields up to 6 g L −1 in static fermentation. Produced BNC was utilized in straightforward catalyst preparation as a solid support for two different transition metals, palladium and copper with metal loading of 20 and 3 wt%, respectively. Sustainable catalysts were applied in the synthesis of valuable fine chemicals, such as biphenyl-4-amine and 4′-fluorobiphenyl-4-amine, used in drug discovery, perfumes and dye industries with excellent product yields of up to 99%. Pd/BNC catalyst was reused 4 times and applied in two consecutive reactions, Suzuki-Miyaura cross-coupling reaction followed by hydrogenation of nitro to amino group while Cu/BNC catalyst was examined in Chan-Lam coupling reaction. Overall, the environmentally benign process of obtaining nanocellulose from biomass, followed by its utilisation as a solid support in metal-catalysed reactions and its recovery has been described. These findings reveal that BNC is a good support material, and it can be used as a support for different catalytic systems.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions
VL  - 129
SP  - 351
EP  - 360
DO  - 10.1016/j.ijbiomac.2019.01.154
ER  - 

@article{
author = "Jeremić, Sanja and Đokić, Lidija and Ajdačić, Vladimir and Božinović, Nina S. and Pavlović, Vladimir D. and Manojlović, Dragan D. and Babu, Ramesh P. and Senthamaraikannan, Ramsankar and Rojas, Orlando and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2019",
abstract = "Bacterial nanocellulose (BNC) emerged as an attractive advanced biomaterial that provides desirable properties such as high strength, lightweight, tailorable surface chemistry, hydrophilicity, and biodegradability. BNC was successfully obtained from a wide range of carbon sources including sugars derived from grass biomass using Komagataeibacter medellinensis ID13488 strain with yields up to 6 g L −1 in static fermentation. Produced BNC was utilized in straightforward catalyst preparation as a solid support for two different transition metals, palladium and copper with metal loading of 20 and 3 wt%, respectively. Sustainable catalysts were applied in the synthesis of valuable fine chemicals, such as biphenyl-4-amine and 4′-fluorobiphenyl-4-amine, used in drug discovery, perfumes and dye industries with excellent product yields of up to 99%. Pd/BNC catalyst was reused 4 times and applied in two consecutive reactions, Suzuki-Miyaura cross-coupling reaction followed by hydrogenation of nitro to amino group while Cu/BNC catalyst was examined in Chan-Lam coupling reaction. Overall, the environmentally benign process of obtaining nanocellulose from biomass, followed by its utilisation as a solid support in metal-catalysed reactions and its recovery has been described. These findings reveal that BNC is a good support material, and it can be used as a support for different catalytic systems.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions",
volume = "129",
pages = "351-360",
doi = "10.1016/j.ijbiomac.2019.01.154"
}

Jeremić, S., Đokić, L., Ajdačić, V., Božinović, N. S., Pavlović, V. D., Manojlović, D. D., Babu, R. P., Senthamaraikannan, R., Rojas, O., Opsenica, I.,& Nikodinović-Runić, J.. (2019). Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions. in International Journal of Biological Macromolecules
Elsevier., 129, 351-360.
https://doi.org/10.1016/j.ijbiomac.2019.01.154

Jeremić S, Đokić L, Ajdačić V, Božinović NS, Pavlović VD, Manojlović DD, Babu RP, Senthamaraikannan R, Rojas O, Opsenica I, Nikodinović-Runić J. Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions. in International Journal of Biological Macromolecules. 2019;129:351-360.
doi:10.1016/j.ijbiomac.2019.01.154 .

Jeremić, Sanja, Đokić, Lidija, Ajdačić, Vladimir, Božinović, Nina S., Pavlović, Vladimir D., Manojlović, Dragan D., Babu, Ramesh P., Senthamaraikannan, Ramsankar, Rojas, Orlando, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions" in International Journal of Biological Macromolecules, 129 (2019):351-360,
https://doi.org/10.1016/j.ijbiomac.2019.01.154 . .

TY  - JOUR
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Duane, Gearoid F.
AU  - Casey, Eoin
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - Nikodinović-Runić, Jasmina
AU  - Murray, Michael
AU  - O'Connor, Kevin E.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1764
AB  - A process for the conversion of post consumer (agricultural) polyethylene (PE) waste to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) is reported here. The thermal treatment of PE in the absence of air (pyrolysis) generated a complex mixture of low molecular weight paraffins with carbon chain lengths from C8 to C32 (PE pyrolysis wax). Several bacterial strains were able to grow and produce PHA from this PE pyrolysis wax. The addition of biosurfactant (rhamnolipids) allowed for greater bacterial growth and PHA accumulation of the tested strains. Some strains were only capable of growth and PHA accumulation in the presence of the biosurfactant. Pseudomonas aeruginosa PAO-1 accumulated the highest level of PHA with almost 25 % of the cell dry weight as PHA when supplied with the PE pyrolysis wax in the presence of rhamnolipids. The change of nitrogen source from ammonium chloride to ammonium nitrate resulted in faster bacterial growth and the earlier onset of PHA accumulation. To our knowledge, this is the first report where PE is used as a starting material for production of a biodegradable polymer.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate
VL  - 98
IS  - 9
SP  - 4223
EP  - 4232
DO  - 10.1007/s00253-013-5489-2
ER  - 

@article{
author = "Guzik, Maciej and Kenny, Shane T. and Duane, Gearoid F. and Casey, Eoin and Woods, Trevor and Babu, Ramesh P. and Nikodinović-Runić, Jasmina and Murray, Michael and O'Connor, Kevin E.",
year = "2014",
abstract = "A process for the conversion of post consumer (agricultural) polyethylene (PE) waste to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) is reported here. The thermal treatment of PE in the absence of air (pyrolysis) generated a complex mixture of low molecular weight paraffins with carbon chain lengths from C8 to C32 (PE pyrolysis wax). Several bacterial strains were able to grow and produce PHA from this PE pyrolysis wax. The addition of biosurfactant (rhamnolipids) allowed for greater bacterial growth and PHA accumulation of the tested strains. Some strains were only capable of growth and PHA accumulation in the presence of the biosurfactant. Pseudomonas aeruginosa PAO-1 accumulated the highest level of PHA with almost 25 % of the cell dry weight as PHA when supplied with the PE pyrolysis wax in the presence of rhamnolipids. The change of nitrogen source from ammonium chloride to ammonium nitrate resulted in faster bacterial growth and the earlier onset of PHA accumulation. To our knowledge, this is the first report where PE is used as a starting material for production of a biodegradable polymer.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate",
volume = "98",
number = "9",
pages = "4223-4232",
doi = "10.1007/s00253-013-5489-2"
}

Guzik, M., Kenny, S. T., Duane, G. F., Casey, E., Woods, T., Babu, R. P., Nikodinović-Runić, J., Murray, M.,& O'Connor, K. E.. (2014). Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 98(9), 4223-4232.
https://doi.org/10.1007/s00253-013-5489-2

Guzik M, Kenny ST, Duane GF, Casey E, Woods T, Babu RP, Nikodinović-Runić J, Murray M, O'Connor KE. Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2014;98(9):4223-4232.
doi:10.1007/s00253-013-5489-2 .

Guzik, Maciej, Kenny, Shane T., Duane, Gearoid F., Casey, Eoin, Woods, Trevor, Babu, Ramesh P., Nikodinović-Runić, Jasmina, Murray, Michael, O'Connor, Kevin E., "Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 98, no. 9 (2014):4223-4232,
https://doi.org/10.1007/s00253-013-5489-2 . .

TY  - JOUR
AU  - Guzik, Maciej
AU  - Narančić, Tanja
AU  - Ilić-Tomić, Tatjana
AU  - Vojnović, Sandra
AU  - Kenny, Shane T.
AU  - Casey, William T.
AU  - Duane, Gearoid F.
AU  - Casey, Eoin
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin E.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1846
AB  - Diverse and elaborate pathways for nutrient utilization, as well as mechanisms to combat unfavourable nutrient conditions make Pseudomonas putida KT2440 a versatile micro-organism able to occupy a range of ecological niches. The fatty acid degradation pathway of P. putida is complex and correlated with biopolymer medium chain length polyhydroxyalkanoate (mcl-PHA) biosynthesis. Little is known about the second step of fatty acid degradation (beta-oxidation) in this strain. In silico analysis of its genome sequence revealed 21 putative acyl-CoA dehydrogenases (ACADs), four of which were functionally characterized through mutagenesis studies. Four mutants with insertionally inactivated ACADs (PP_1893, PP_2039, PP_2048 and PP_2437) grew and accumulated mcl-PHA on a range of fatty acids as the sole source of carbon and energy. Their ability to grow and accumulate biopolymer was differentially negatively affected on various fatty acids, in comparison to the wild-type strain. Inactive PP_2437 exhibited a pattern of reduced growth and PHA accumulation when fatty acids with lengths of 10 to 14 carbon chains were used as substrates. Recombinant expression and biochemical characterization of the purified protein allowed functional annotation in P. putida KT2440 as an ACAD showing clear preference for dodecanoyl-CoA ester as a substrate and optimum activity at 30 degrees C and pH 6.5-7.
PB  - Soc General Microbiology, Reading
T2  - Microbiology, SGM / Society for General Microbiology
T1  - Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids
VL  - 160
SP  - 1760
EP  - 1771
DO  - 10.1099/mic.0.078758-0
ER  - 

@article{
author = "Guzik, Maciej and Narančić, Tanja and Ilić-Tomić, Tatjana and Vojnović, Sandra and Kenny, Shane T. and Casey, William T. and Duane, Gearoid F. and Casey, Eoin and Woods, Trevor and Babu, Ramesh P. and Nikodinović-Runić, Jasmina and O'Connor, Kevin E.",
year = "2014",
abstract = "Diverse and elaborate pathways for nutrient utilization, as well as mechanisms to combat unfavourable nutrient conditions make Pseudomonas putida KT2440 a versatile micro-organism able to occupy a range of ecological niches. The fatty acid degradation pathway of P. putida is complex and correlated with biopolymer medium chain length polyhydroxyalkanoate (mcl-PHA) biosynthesis. Little is known about the second step of fatty acid degradation (beta-oxidation) in this strain. In silico analysis of its genome sequence revealed 21 putative acyl-CoA dehydrogenases (ACADs), four of which were functionally characterized through mutagenesis studies. Four mutants with insertionally inactivated ACADs (PP_1893, PP_2039, PP_2048 and PP_2437) grew and accumulated mcl-PHA on a range of fatty acids as the sole source of carbon and energy. Their ability to grow and accumulate biopolymer was differentially negatively affected on various fatty acids, in comparison to the wild-type strain. Inactive PP_2437 exhibited a pattern of reduced growth and PHA accumulation when fatty acids with lengths of 10 to 14 carbon chains were used as substrates. Recombinant expression and biochemical characterization of the purified protein allowed functional annotation in P. putida KT2440 as an ACAD showing clear preference for dodecanoyl-CoA ester as a substrate and optimum activity at 30 degrees C and pH 6.5-7.",
publisher = "Soc General Microbiology, Reading",
journal = "Microbiology, SGM / Society for General Microbiology",
title = "Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids",
volume = "160",
pages = "1760-1771",
doi = "10.1099/mic.0.078758-0"
}

Guzik, M., Narančić, T., Ilić-Tomić, T., Vojnović, S., Kenny, S. T., Casey, W. T., Duane, G. F., Casey, E., Woods, T., Babu, R. P., Nikodinović-Runić, J.,& O'Connor, K. E.. (2014). Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids. in Microbiology, SGM / Society for General Microbiology
Soc General Microbiology, Reading., 160, 1760-1771.
https://doi.org/10.1099/mic.0.078758-0

Guzik M, Narančić T, Ilić-Tomić T, Vojnović S, Kenny ST, Casey WT, Duane GF, Casey E, Woods T, Babu RP, Nikodinović-Runić J, O'Connor KE. Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids. in Microbiology, SGM / Society for General Microbiology. 2014;160:1760-1771.
doi:10.1099/mic.0.078758-0 .

Guzik, Maciej, Narančić, Tanja, Ilić-Tomić, Tatjana, Vojnović, Sandra, Kenny, Shane T., Casey, William T., Duane, Gearoid F., Casey, Eoin, Woods, Trevor, Babu, Ramesh P., Nikodinović-Runić, Jasmina, O'Connor, Kevin E., "Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids" in Microbiology, SGM / Society for General Microbiology, 160 (2014):1760-1771,
https://doi.org/10.1099/mic.0.078758-0 . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Babu, Ramesh P.
AU  - Werker, Alan
AU  - Connor, Kevin E. O.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1368
AB  - Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.
PB  - Elsevier Academic Press Inc, San Diego
T2  - Advances in Applied Microbiology
T1  - Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria
VL  - 84
SP  - 139
EP  - 200
DO  - 10.1016/B978-0-12-407673-0.00004-7
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Guzik, Maciej and Kenny, Shane T. and Babu, Ramesh P. and Werker, Alan and Connor, Kevin E. O.",
year = "2013",
abstract = "Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.",
publisher = "Elsevier Academic Press Inc, San Diego",
journal = "Advances in Applied Microbiology",
title = "Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria",
volume = "84",
pages = "139-200",
doi = "10.1016/B978-0-12-407673-0.00004-7"
}

Nikodinović-Runić, J., Guzik, M., Kenny, S. T., Babu, R. P., Werker, A.,& Connor, K. E. O.. (2013). Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria. in Advances in Applied Microbiology
Elsevier Academic Press Inc, San Diego., 84, 139-200.
https://doi.org/10.1016/B978-0-12-407673-0.00004-7

Nikodinović-Runić J, Guzik M, Kenny ST, Babu RP, Werker A, Connor KEO. Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria. in Advances in Applied Microbiology. 2013;84:139-200.
doi:10.1016/B978-0-12-407673-0.00004-7 .

Nikodinović-Runić, Jasmina, Guzik, Maciej, Kenny, Shane T., Babu, Ramesh P., Werker, Alan, Connor, Kevin E. O., "Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria" in Advances in Applied Microbiology, 84 (2013):139-200,
https://doi.org/10.1016/B978-0-12-407673-0.00004-7 . .

TY  - JOUR
AU  - O'Connor, Stephen
AU  - Szwej, Emilia
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Aisling
AU  - Byrne, Annette T.
AU  - Devocelle, Marc
AU  - O'Donovan, Norma
AU  - Gallagher, William M.
AU  - Babu, Ramesh P.
AU  - Kenny, Shane T.
AU  - Zinn, Manfred
AU  - Zulian, Qun Ren
AU  - O'Connor, Kevin E.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1609
AB  - The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values compared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range.
PB  - Elsevier Sci Ltd, Oxford
T2  - Biomaterials
T1  - The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate
VL  - 34
IS  - 11
SP  - 2710
EP  - 2718
DO  - 10.1016/j.biomaterials.2012.12.032
ER  - 

@article{
author = "O'Connor, Stephen and Szwej, Emilia and Nikodinović-Runić, Jasmina and O'Connor, Aisling and Byrne, Annette T. and Devocelle, Marc and O'Donovan, Norma and Gallagher, William M. and Babu, Ramesh P. and Kenny, Shane T. and Zinn, Manfred and Zulian, Qun Ren and O'Connor, Kevin E.",
year = "2013",
abstract = "The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values compared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Biomaterials",
title = "The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate",
volume = "34",
number = "11",
pages = "2710-2718",
doi = "10.1016/j.biomaterials.2012.12.032"
}

O'Connor, S., Szwej, E., Nikodinović-Runić, J., O'Connor, A., Byrne, A. T., Devocelle, M., O'Donovan, N., Gallagher, W. M., Babu, R. P., Kenny, S. T., Zinn, M., Zulian, Q. R.,& O'Connor, K. E.. (2013). The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. in Biomaterials
Elsevier Sci Ltd, Oxford., 34(11), 2710-2718.
https://doi.org/10.1016/j.biomaterials.2012.12.032

O'Connor S, Szwej E, Nikodinović-Runić J, O'Connor A, Byrne AT, Devocelle M, O'Donovan N, Gallagher WM, Babu RP, Kenny ST, Zinn M, Zulian QR, O'Connor KE. The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. in Biomaterials. 2013;34(11):2710-2718.
doi:10.1016/j.biomaterials.2012.12.032 .

O'Connor, Stephen, Szwej, Emilia, Nikodinović-Runić, Jasmina, O'Connor, Aisling, Byrne, Annette T., Devocelle, Marc, O'Donovan, Norma, Gallagher, William M., Babu, Ramesh P., Kenny, Shane T., Zinn, Manfred, Zulian, Qun Ren, O'Connor, Kevin E., "The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate" in Biomaterials, 34, no. 11 (2013):2710-2718,
https://doi.org/10.1016/j.biomaterials.2012.12.032 . .

TY  - DATA
AU  - O'Connor, Stephen
AU  - Szwej, Emilia
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Aisling
AU  - Byrne, Annette T.
AU  - Devocelle, Marc
AU  - O'Donovan, Norma
AU  - Gallagher, William M.
AU  - Babu, Ramesh P.
AU  - Kenny, Shane T.
AU  - Zinn, Manfred
AU  - Zulian, Qun Ren
AU  - O'Connor, Kevin E.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3550
PB  - Elsevier Sci Ltd, Oxford
T2  - Biomaterials
T1  - Supplementary data for article: O’Connor, S.; Szwej, E.; Nikodinović-Runić, J.; O’Connor, A.; Byrne, A. T.; Devocelle, M.; O’Donovan, N.; Gallagher, W. M.; Babu, R. P.; Kenny, S. T.; et al. The Anti-Cancer Activity of a Cationic Anti-Microbial Peptide Derived from Monomers of Polyhydroxyalkanoate. Biomaterials 2013, 34 (11), 2710–2718. https://doi.org/10.1016/j.biomaterials.2012.12.032
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3550
ER  - 

@misc{
author = "O'Connor, Stephen and Szwej, Emilia and Nikodinović-Runić, Jasmina and O'Connor, Aisling and Byrne, Annette T. and Devocelle, Marc and O'Donovan, Norma and Gallagher, William M. and Babu, Ramesh P. and Kenny, Shane T. and Zinn, Manfred and Zulian, Qun Ren and O'Connor, Kevin E.",
year = "2013",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Biomaterials",
title = "Supplementary data for article: O’Connor, S.; Szwej, E.; Nikodinović-Runić, J.; O’Connor, A.; Byrne, A. T.; Devocelle, M.; O’Donovan, N.; Gallagher, W. M.; Babu, R. P.; Kenny, S. T.; et al. The Anti-Cancer Activity of a Cationic Anti-Microbial Peptide Derived from Monomers of Polyhydroxyalkanoate. Biomaterials 2013, 34 (11), 2710–2718. https://doi.org/10.1016/j.biomaterials.2012.12.032",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3550"
}

O'Connor, S., Szwej, E., Nikodinović-Runić, J., O'Connor, A., Byrne, A. T., Devocelle, M., O'Donovan, N., Gallagher, W. M., Babu, R. P., Kenny, S. T., Zinn, M., Zulian, Q. R.,& O'Connor, K. E.. (2013). Supplementary data for article: O’Connor, S.; Szwej, E.; Nikodinović-Runić, J.; O’Connor, A.; Byrne, A. T.; Devocelle, M.; O’Donovan, N.; Gallagher, W. M.; Babu, R. P.; Kenny, S. T.; et al. The Anti-Cancer Activity of a Cationic Anti-Microbial Peptide Derived from Monomers of Polyhydroxyalkanoate. Biomaterials 2013, 34 (11), 2710–2718. https://doi.org/10.1016/j.biomaterials.2012.12.032. in Biomaterials
Elsevier Sci Ltd, Oxford..
https://hdl.handle.net/21.15107/rcub_cherry_3550

O'Connor S, Szwej E, Nikodinović-Runić J, O'Connor A, Byrne AT, Devocelle M, O'Donovan N, Gallagher WM, Babu RP, Kenny ST, Zinn M, Zulian QR, O'Connor KE. Supplementary data for article: O’Connor, S.; Szwej, E.; Nikodinović-Runić, J.; O’Connor, A.; Byrne, A. T.; Devocelle, M.; O’Donovan, N.; Gallagher, W. M.; Babu, R. P.; Kenny, S. T.; et al. The Anti-Cancer Activity of a Cationic Anti-Microbial Peptide Derived from Monomers of Polyhydroxyalkanoate. Biomaterials 2013, 34 (11), 2710–2718. https://doi.org/10.1016/j.biomaterials.2012.12.032. in Biomaterials. 2013;.
https://hdl.handle.net/21.15107/rcub_cherry_3550 .

O'Connor, Stephen, Szwej, Emilia, Nikodinović-Runić, Jasmina, O'Connor, Aisling, Byrne, Annette T., Devocelle, Marc, O'Donovan, Norma, Gallagher, William M., Babu, Ramesh P., Kenny, Shane T., Zinn, Manfred, Zulian, Qun Ren, O'Connor, Kevin E., "Supplementary data for article: O’Connor, S.; Szwej, E.; Nikodinović-Runić, J.; O’Connor, A.; Byrne, A. T.; Devocelle, M.; O’Donovan, N.; Gallagher, W. M.; Babu, R. P.; Kenny, S. T.; et al. The Anti-Cancer Activity of a Cationic Anti-Microbial Peptide Derived from Monomers of Polyhydroxyalkanoate. Biomaterials 2013, 34 (11), 2710–2718. https://doi.org/10.1016/j.biomaterials.2012.12.032" in Biomaterials (2013),
https://hdl.handle.net/21.15107/rcub_cherry_3550 .

TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - O'Connor, Kevin E.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1319
AB  - Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate
VL  - 95
IS  - 3
SP  - 623
EP  - 633
DO  - 10.1007/s00253-012-4058-4
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh P. and O'Connor, Kevin E.",
year = "2012",
abstract = "Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate",
volume = "95",
number = "3",
pages = "623-633",
doi = "10.1007/s00253-012-4058-4"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R. P.,& O'Connor, K. E.. (2012). Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 95(3), 623-633.
https://doi.org/10.1007/s00253-012-4058-4

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu RP, O'Connor KE. Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2012;95(3):623-633.
doi:10.1007/s00253-012-4058-4 .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh P., O'Connor, Kevin E., "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 95, no. 3 (2012):623-633,
https://doi.org/10.1007/s00253-012-4058-4 . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Martin, Leona B.
AU  - Babu, Ramesh P.
AU  - Blau, Werner
AU  - O'Connor, Kevin E.
PY  - 2009
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1008
AB  - Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates  gt  30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - FEMS Microbiology Letters / Federation of European Microbiological Societies
T1  - Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6
VL  - 298
IS  - 2
SP  - 174
EP  - 183
DO  - 10.1111/j.1574-6968.2009.01716.x
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Martin, Leona B. and Babu, Ramesh P. and Blau, Werner and O'Connor, Kevin E.",
year = "2009",
abstract = "Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates  gt  30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "FEMS Microbiology Letters / Federation of European Microbiological Societies",
title = "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6",
volume = "298",
number = "2",
pages = "174-183",
doi = "10.1111/j.1574-6968.2009.01716.x"
}

Nikodinović-Runić, J., Martin, L. B., Babu, R. P., Blau, W.,& O'Connor, K. E.. (2009). Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters / Federation of European Microbiological Societies
Wiley-Blackwell Publishing, Inc, Malden., 298(2), 174-183.
https://doi.org/10.1111/j.1574-6968.2009.01716.x

Nikodinović-Runić J, Martin LB, Babu RP, Blau W, O'Connor KE. Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters / Federation of European Microbiological Societies. 2009;298(2):174-183.
doi:10.1111/j.1574-6968.2009.01716.x .

Nikodinović-Runić, Jasmina, Martin, Leona B., Babu, Ramesh P., Blau, Werner, O'Connor, Kevin E., "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6" in FEMS Microbiology Letters / Federation of European Microbiological Societies, 298, no. 2 (2009):174-183,
https://doi.org/10.1111/j.1574-6968.2009.01716.x . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Kenny, Shane T.
AU  - Babu, Ramesh P.
AU  - Woods, Trevor
AU  - Blau, Werner J.
AU  - O'Connor, Kevin E.
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/967
AB  - Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate
VL  - 80
IS  - 4
SP  - 665
EP  - 673
DO  - 10.1007/s00253-008-1593-0
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Kenny, Shane T. and Babu, Ramesh P. and Woods, Trevor and Blau, Werner J. and O'Connor, Kevin E.",
year = "2008",
abstract = "Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate",
volume = "80",
number = "4",
pages = "665-673",
doi = "10.1007/s00253-008-1593-0"
}

Nikodinović-Runić, J., Kenny, S. T., Babu, R. P., Woods, T., Blau, W. J.,& O'Connor, K. E.. (2008). The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 80(4), 665-673.
https://doi.org/10.1007/s00253-008-1593-0

Nikodinović-Runić J, Kenny ST, Babu RP, Woods T, Blau WJ, O'Connor KE. The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2008;80(4):665-673.
doi:10.1007/s00253-008-1593-0 .

Nikodinović-Runić, Jasmina, Kenny, Shane T., Babu, Ramesh P., Woods, Trevor, Blau, Werner J., O'Connor, Kevin E., "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 80, no. 4 (2008):665-673,
https://doi.org/10.1007/s00253-008-1593-0 . .

TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - Keely, Chris M.
AU  - Blau, Werner
AU  - O'Connor, Kevin E.
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/978
AB  - The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.
PB  - Amer Chemical Soc, Washington
T2  - Environmental Science and Technology
T1  - Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
VL  - 42
IS  - 20
SP  - 7696
EP  - 7701
DO  - 10.1021/es801010e
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh P. and Keely, Chris M. and Blau, Werner and O'Connor, Kevin E.",
year = "2008",
abstract = "The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.",
publisher = "Amer Chemical Soc, Washington",
journal = "Environmental Science and Technology",
title = "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)",
volume = "42",
number = "20",
pages = "7696-7701",
doi = "10.1021/es801010e"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R. P., Keely, C. M., Blau, W.,& O'Connor, K. E.. (2008). Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science and Technology
Amer Chemical Soc, Washington., 42(20), 7696-7701.
https://doi.org/10.1021/es801010e

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu RP, Keely CM, Blau W, O'Connor KE. Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science and Technology. 2008;42(20):7696-7701.
doi:10.1021/es801010e .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh P., Keely, Chris M., Blau, Werner, O'Connor, Kevin E., "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)" in Environmental Science and Technology, 42, no. 20 (2008):7696-7701,
https://doi.org/10.1021/es801010e . .