TY  - JOUR
AU  - Miljević, Čedo
AU  - Nikolić-Kokić, Aleksandra
AU  - Nikolić, Milan
AU  - Niketić, Vesna
AU  - Spasić, Mihajlo B.
AU  - Lecic-Tosevski, Dusica
AU  - Blagojević, Duško P.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1581
AB  - Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on the activity of antioxidant defence enzymes in human erythrocytes in vitro. Methods Cu,Zn-superoxide dismutase (SOD1), catalase (CAT), selenium-dependent glutathione peroxidase and glutathione reductase activities were determined after drugs incubation with blood of 15 apparently healthy non-smoking male volunteers (ages 2339) for 1?h at 37?degrees C. Results A statistically significant increase in SOD1 activity was found in samples incubated with aripiprazole (p? lt ?0.01) and quetiapine (p? lt ?0.05) compared with incubated control. SOD1 activity profile following native polyacrylamide gel electrophoresis indicates that aripiprazole and quetiapine protect enzyme activity from inhibition with hydrogen peroxide. Our results showed that sertindole decreases activity of CAT comparing with control non-treated erythrocytes. Moreover, in sertindole treated erythrocytes, negative correlation between SOD1 and glutathione peroxidase activities was found. Increased amount of hydrogen peroxide in such situation may leave erythrocytes and transform their role in circulation from anti-oxidative to pro-oxidative. Conclusions Our results indicate that mechanism through sertindole could express its in vivo toxic effects and point toward possible (neuro)protective effects of aripiprazole and quetiapine.
PB  - Wiley, Hoboken
T2  - Human Psychopharmacology: Clinical and Experimental
T1  - Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study)
VL  - 28
IS  - 1
SP  - 1
EP  - 6
DO  - 10.1002/hup.2272
ER  - 

@article{
author = "Miljević, Čedo and Nikolić-Kokić, Aleksandra and Nikolić, Milan and Niketić, Vesna and Spasić, Mihajlo B. and Lecic-Tosevski, Dusica and Blagojević, Duško P.",
year = "2013",
abstract = "Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on the activity of antioxidant defence enzymes in human erythrocytes in vitro. Methods Cu,Zn-superoxide dismutase (SOD1), catalase (CAT), selenium-dependent glutathione peroxidase and glutathione reductase activities were determined after drugs incubation with blood of 15 apparently healthy non-smoking male volunteers (ages 2339) for 1?h at 37?degrees C. Results A statistically significant increase in SOD1 activity was found in samples incubated with aripiprazole (p? lt ?0.01) and quetiapine (p? lt ?0.05) compared with incubated control. SOD1 activity profile following native polyacrylamide gel electrophoresis indicates that aripiprazole and quetiapine protect enzyme activity from inhibition with hydrogen peroxide. Our results showed that sertindole decreases activity of CAT comparing with control non-treated erythrocytes. Moreover, in sertindole treated erythrocytes, negative correlation between SOD1 and glutathione peroxidase activities was found. Increased amount of hydrogen peroxide in such situation may leave erythrocytes and transform their role in circulation from anti-oxidative to pro-oxidative. Conclusions Our results indicate that mechanism through sertindole could express its in vivo toxic effects and point toward possible (neuro)protective effects of aripiprazole and quetiapine.",
publisher = "Wiley, Hoboken",
journal = "Human Psychopharmacology: Clinical and Experimental",
title = "Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study)",
volume = "28",
number = "1",
pages = "1-6",
doi = "10.1002/hup.2272"
}

Miljević, Č., Nikolić-Kokić, A., Nikolić, M., Niketić, V., Spasić, M. B., Lecic-Tosevski, D.,& Blagojević, D. P.. (2013). Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study). in Human Psychopharmacology: Clinical and Experimental
Wiley, Hoboken., 28(1), 1-6.
https://doi.org/10.1002/hup.2272

Miljević Č, Nikolić-Kokić A, Nikolić M, Niketić V, Spasić MB, Lecic-Tosevski D, Blagojević DP. Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study). in Human Psychopharmacology: Clinical and Experimental. 2013;28(1):1-6.
doi:10.1002/hup.2272 .

Miljević, Čedo, Nikolić-Kokić, Aleksandra, Nikolić, Milan, Niketić, Vesna, Spasić, Mihajlo B., Lecic-Tosevski, Dusica, Blagojević, Duško P., "Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes (in vitro study)" in Human Psychopharmacology: Clinical and Experimental, 28, no. 1 (2013):1-6,
https://doi.org/10.1002/hup.2272 . .

TY  - JOUR
AU  - Mijevic, Cedo
AU  - Nikolić, Milan
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R.
AU  - Niketić, Vesna
AU  - Lecic-Tosevski, Dusica
AU  - Spasić, Mihajlo B.
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1059
AB  - Objective: Despite clozapine's unique effectiveness in patients with schizophrenia, a number of adverse effects have been recognised including abnormalities in lipid and glucose metabolisms. A high clozapine level in red blood cells (RBCs) and disturbed anti-oxidant enzyme activities in blood from schizophrenic patients prompted us to investigate lipid status and anti-oxidant enzyme defence in the blood of chronic schizophrenic patients on long-term clozapine therapy. Methods: Plasma lipids, RBC anti-oxidant enzyme activities and haemoglobin (Hb) content were measured using established procedures in a group of eighteen chronically-medicated (average 630 days of therapy) schizophrenic patients receiving clozapine (average dose of 295 mg/day) and data were compared with those from a group of eighteen well-matched normal controls. Results: Significantly higher levels of plasma triglycerides (by 47%, p lt 0.01) and total cholesterol and phospholipids (by 8% and 11%, respectively p lt 0.05) in patients were found. CuZn-superoxide dismutase (SOD1) activity was markedly higher (by 35%, p lt 0.001) while selenium-dependent glutathione peroxidase (GSH-Px1) activity was markedly lower (by 41%, p lt 0.001) in patients. In addition, metHb and HbA1c levels in patients were significantly higher (by 58% and 25%. respectively p lt 0.001). SOD1 activity was negatively correlated (p lt 0.001) to GSH-Px1 activity in patients. Conclusions:The findings support the view that ongoing oxidative stress may be a mechanism by which clozapine induces some adverse effects that increase the risk of diabetes and metabolic syndrome. If valid, this would indicate that in parallel with long-term clozapine treatment, schizophrenic patients could be encouraged to make some lifestyle changes to limit the detrimental effects of the medication. (C) 2009 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Progress in Neuro-psychopharmacology and Biological Psychiatry
T1  - Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients
VL  - 34
IS  - 2
SP  - 303
EP  - 307
DO  - 10.1016/j.pnpbp.2009.11.024
ER  - 

@article{
author = "Mijevic, Cedo and Nikolić, Milan and Nikolić-Kokić, Aleksandra and Jones, David R. and Niketić, Vesna and Lecic-Tosevski, Dusica and Spasić, Mihajlo B.",
year = "2010",
abstract = "Objective: Despite clozapine's unique effectiveness in patients with schizophrenia, a number of adverse effects have been recognised including abnormalities in lipid and glucose metabolisms. A high clozapine level in red blood cells (RBCs) and disturbed anti-oxidant enzyme activities in blood from schizophrenic patients prompted us to investigate lipid status and anti-oxidant enzyme defence in the blood of chronic schizophrenic patients on long-term clozapine therapy. Methods: Plasma lipids, RBC anti-oxidant enzyme activities and haemoglobin (Hb) content were measured using established procedures in a group of eighteen chronically-medicated (average 630 days of therapy) schizophrenic patients receiving clozapine (average dose of 295 mg/day) and data were compared with those from a group of eighteen well-matched normal controls. Results: Significantly higher levels of plasma triglycerides (by 47%, p lt 0.01) and total cholesterol and phospholipids (by 8% and 11%, respectively p lt 0.05) in patients were found. CuZn-superoxide dismutase (SOD1) activity was markedly higher (by 35%, p lt 0.001) while selenium-dependent glutathione peroxidase (GSH-Px1) activity was markedly lower (by 41%, p lt 0.001) in patients. In addition, metHb and HbA1c levels in patients were significantly higher (by 58% and 25%. respectively p lt 0.001). SOD1 activity was negatively correlated (p lt 0.001) to GSH-Px1 activity in patients. Conclusions:The findings support the view that ongoing oxidative stress may be a mechanism by which clozapine induces some adverse effects that increase the risk of diabetes and metabolic syndrome. If valid, this would indicate that in parallel with long-term clozapine treatment, schizophrenic patients could be encouraged to make some lifestyle changes to limit the detrimental effects of the medication. (C) 2009 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Progress in Neuro-psychopharmacology and Biological Psychiatry",
title = "Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients",
volume = "34",
number = "2",
pages = "303-307",
doi = "10.1016/j.pnpbp.2009.11.024"
}

Mijevic, C., Nikolić, M., Nikolić-Kokić, A., Jones, D. R., Niketić, V., Lecic-Tosevski, D.,& Spasić, M. B.. (2010). Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients. in Progress in Neuro-psychopharmacology and Biological Psychiatry
Pergamon-Elsevier Science Ltd, Oxford., 34(2), 303-307.
https://doi.org/10.1016/j.pnpbp.2009.11.024

Mijevic C, Nikolić M, Nikolić-Kokić A, Jones DR, Niketić V, Lecic-Tosevski D, Spasić MB. Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients. in Progress in Neuro-psychopharmacology and Biological Psychiatry. 2010;34(2):303-307.
doi:10.1016/j.pnpbp.2009.11.024 .

Mijevic, Cedo, Nikolić, Milan, Nikolić-Kokić, Aleksandra, Jones, David R., Niketić, Vesna, Lecic-Tosevski, Dusica, Spasić, Mihajlo B., "Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients" in Progress in Neuro-psychopharmacology and Biological Psychiatry, 34, no. 2 (2010):303-307,
https://doi.org/10.1016/j.pnpbp.2009.11.024 . .