TY  - JOUR
AU  - Jevtic, Bojan
AU  - Djedovic, Neda
AU  - Stanisavljevic, Suzana
AU  - Gašić, Uroš M.
AU  - Mišić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2537
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
SP  - 249
EP  - 262
DO  - 10.1016/j.jff.2017.07.060
ER  - 

@article{
author = "Jevtic, Bojan and Djedovic, Neda and Stanisavljevic, Suzana and Gašić, Uroš M. and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
pages = "249-262",
doi = "10.1016/j.jff.2017.07.060"
}

Jevtic, B., Djedovic, N., Stanisavljevic, S., Gašić, U. M., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtic B, Djedovic N, Stanisavljevic S, Gašić UM, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtic, Bojan, Djedovic, Neda, Stanisavljevic, Suzana, Gašić, Uroš M., Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Mojic, Marija
AU  - Savić, Aleksandar
AU  - Arion, Vladimir B.
AU  - Bulatović, Mirna Z.
AU  - Poljarević, Jelena
AU  - Miljković, Đorđe
AU  - Sabo, Tibor
AU  - Mijatovic, Sanja
AU  - Maksimovic-Ivanic, Danijela
AU  - Grgurić-Šipka, Sanja
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1450
AB  - Two p-cymene ruthenium chlorido complexes containing isobutyl (C1) and isoamyl (C2) esters of (S,S)ethylenediamine-N,N'-di-2-(3-cyclohexyl) propanoic acid as ligands were prepared from p-cymene ruthenium dichloride dimer and corresponding ester. All compounds have been characterized by elemental analysis, IR, ESI-MS, H-1 and C-13 NMR spectroscopy. Single crystal X-ray structure diffraction analysis of C1 shows the usual piano-stool geometry of complexes, with coordination of ester ligand via nitrogen donor atoms. Ligands exhibit moderate anticancer activity (IC50  gt  50 mu M), while the complexes were significantly more cytotoxic towards various cancer cell lines, including B16, A375, HCT116, A549 and MCF7 cells (IC50 min.-max. 2.9-8.0 mu M). We stress that cisplatin resistant HCT116 cell line was highly sensitive to the treatment with C1 and C2 (IC50 values: 4.4 and 5.5 mu M versus IC50  gt  120 mu M for cisplatin). In parallel, primary fibroblasts-MRC-5 were remarkably less affected by these compounds. (C) 2013 Elsevier B. V. All rights reserved.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Organometallic Chemistry
T1  - Synthesis, X-ray structure and strong in vitro cytotoxicity of novel organoruthenium complexes
VL  - 749
SP  - 142
EP  - 149
DO  - 10.1016/j.jorganchem.2013.08.041
ER  - 

@article{
author = "Mojic, Marija and Savić, Aleksandar and Arion, Vladimir B. and Bulatović, Mirna Z. and Poljarević, Jelena and Miljković, Đorđe and Sabo, Tibor and Mijatovic, Sanja and Maksimovic-Ivanic, Danijela and Grgurić-Šipka, Sanja",
year = "2014",
abstract = "Two p-cymene ruthenium chlorido complexes containing isobutyl (C1) and isoamyl (C2) esters of (S,S)ethylenediamine-N,N'-di-2-(3-cyclohexyl) propanoic acid as ligands were prepared from p-cymene ruthenium dichloride dimer and corresponding ester. All compounds have been characterized by elemental analysis, IR, ESI-MS, H-1 and C-13 NMR spectroscopy. Single crystal X-ray structure diffraction analysis of C1 shows the usual piano-stool geometry of complexes, with coordination of ester ligand via nitrogen donor atoms. Ligands exhibit moderate anticancer activity (IC50  gt  50 mu M), while the complexes were significantly more cytotoxic towards various cancer cell lines, including B16, A375, HCT116, A549 and MCF7 cells (IC50 min.-max. 2.9-8.0 mu M). We stress that cisplatin resistant HCT116 cell line was highly sensitive to the treatment with C1 and C2 (IC50 values: 4.4 and 5.5 mu M versus IC50  gt  120 mu M for cisplatin). In parallel, primary fibroblasts-MRC-5 were remarkably less affected by these compounds. (C) 2013 Elsevier B. V. All rights reserved.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Organometallic Chemistry",
title = "Synthesis, X-ray structure and strong in vitro cytotoxicity of novel organoruthenium complexes",
volume = "749",
pages = "142-149",
doi = "10.1016/j.jorganchem.2013.08.041"
}

Mojic, M., Savić, A., Arion, V. B., Bulatović, M. Z., Poljarević, J., Miljković, Đ., Sabo, T., Mijatovic, S., Maksimovic-Ivanic, D.,& Grgurić-Šipka, S.. (2014). Synthesis, X-ray structure and strong in vitro cytotoxicity of novel organoruthenium complexes. in Journal of Organometallic Chemistry
Elsevier Science Sa, Lausanne., 749, 142-149.
https://doi.org/10.1016/j.jorganchem.2013.08.041

Mojic M, Savić A, Arion VB, Bulatović MZ, Poljarević J, Miljković Đ, Sabo T, Mijatovic S, Maksimovic-Ivanic D, Grgurić-Šipka S. Synthesis, X-ray structure and strong in vitro cytotoxicity of novel organoruthenium complexes. in Journal of Organometallic Chemistry. 2014;749:142-149.
doi:10.1016/j.jorganchem.2013.08.041 .

Mojic, Marija, Savić, Aleksandar, Arion, Vladimir B., Bulatović, Mirna Z., Poljarević, Jelena, Miljković, Đorđe, Sabo, Tibor, Mijatovic, Sanja, Maksimovic-Ivanic, Danijela, Grgurić-Šipka, Sanja, "Synthesis, X-ray structure and strong in vitro cytotoxicity of novel organoruthenium complexes" in Journal of Organometallic Chemistry, 749 (2014):142-149,
https://doi.org/10.1016/j.jorganchem.2013.08.041 . .

TY  - JOUR
AU  - Mihajlović-Lalić, Ljiljana
AU  - Savić, Aleksandar
AU  - Poljarević, Jelena
AU  - Vučković, Ivan M.
AU  - Mojic, Marija
AU  - Bulatović, Mirna Z.
AU  - Maksimovic-Ivanic, Danijela
AU  - Mijatovic, Sanja
AU  - Kaluđerović, Goran N.
AU  - Stošić-Grujičić, Stanislava D.
AU  - Miljković, Đorđe
AU  - Grgurić-Šipka, Sanja
AU  - Sabo, Tibor
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1283
AB  - This paper focuses on the synthesis, characterization and biological activity of new N,N'-methylene modified cyclohexyl ethylenediamine-N,N'-diacetate (edda)-type ligands and their Pt(IV) complexes. Both the ligands and complexes were characterized by infrared, UV-vis, ESI-MS, 1D (H-1, C-13, Pt-195) and 2D (COSY, HSQC, HMBC) NMR spectroscopy and elemental analysis. The possible correlation between the reduction potentials and the cytotoxicity of the complexes was examined. The potential antitumoral activity of all compounds was tested in vitro on human melanoma A375, human glioblastoma U251, human prostate cancer PC3, human colon cancer HCT116, mouse melanoma B16 and mouse colon cancer CT26CL25 cells, as well as primary fibroblasts and keratinocytes. The results obtained revealed strong antitumor potential of the newly synthesized drugs with preserved efficacy against cisplatin resistant lines and less toxicity towards nonmalignant counterparts. The mechanism found to be responsible for the observed tumoricidal action of each synthesized compound was induction of apoptosis generally accompanied with caspase activation. Taken together, the effective response to the treatment of a wide range of different cell lines, including cisplatin resistant subclones, as well as induction of apoptosis, as the mechanism suggested to be the most desirable way of eliminating malignant cells, represents a great advantage of this novel group of drugs in comparison to other members in this metallo-drug family. (C) 2012 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - Novel methylene modified cyclohexyl ethylenediamine-N,N '-diacetate ligands and their platinum(IV) complexes. Influence on biological activity
VL  - 109
SP  - 40
EP  - 48
DO  - 10.1016/j.jinorgbio.2012.01.012
ER  - 

@article{
author = "Mihajlović-Lalić, Ljiljana and Savić, Aleksandar and Poljarević, Jelena and Vučković, Ivan M. and Mojic, Marija and Bulatović, Mirna Z. and Maksimovic-Ivanic, Danijela and Mijatovic, Sanja and Kaluđerović, Goran N. and Stošić-Grujičić, Stanislava D. and Miljković, Đorđe and Grgurić-Šipka, Sanja and Sabo, Tibor",
year = "2012",
abstract = "This paper focuses on the synthesis, characterization and biological activity of new N,N'-methylene modified cyclohexyl ethylenediamine-N,N'-diacetate (edda)-type ligands and their Pt(IV) complexes. Both the ligands and complexes were characterized by infrared, UV-vis, ESI-MS, 1D (H-1, C-13, Pt-195) and 2D (COSY, HSQC, HMBC) NMR spectroscopy and elemental analysis. The possible correlation between the reduction potentials and the cytotoxicity of the complexes was examined. The potential antitumoral activity of all compounds was tested in vitro on human melanoma A375, human glioblastoma U251, human prostate cancer PC3, human colon cancer HCT116, mouse melanoma B16 and mouse colon cancer CT26CL25 cells, as well as primary fibroblasts and keratinocytes. The results obtained revealed strong antitumor potential of the newly synthesized drugs with preserved efficacy against cisplatin resistant lines and less toxicity towards nonmalignant counterparts. The mechanism found to be responsible for the observed tumoricidal action of each synthesized compound was induction of apoptosis generally accompanied with caspase activation. Taken together, the effective response to the treatment of a wide range of different cell lines, including cisplatin resistant subclones, as well as induction of apoptosis, as the mechanism suggested to be the most desirable way of eliminating malignant cells, represents a great advantage of this novel group of drugs in comparison to other members in this metallo-drug family. (C) 2012 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "Novel methylene modified cyclohexyl ethylenediamine-N,N '-diacetate ligands and their platinum(IV) complexes. Influence on biological activity",
volume = "109",
pages = "40-48",
doi = "10.1016/j.jinorgbio.2012.01.012"
}

Mihajlović-Lalić, L., Savić, A., Poljarević, J., Vučković, I. M., Mojic, M., Bulatović, M. Z., Maksimovic-Ivanic, D., Mijatovic, S., Kaluđerović, G. N., Stošić-Grujičić, S. D., Miljković, Đ., Grgurić-Šipka, S.,& Sabo, T.. (2012). Novel methylene modified cyclohexyl ethylenediamine-N,N '-diacetate ligands and their platinum(IV) complexes. Influence on biological activity. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 109, 40-48.
https://doi.org/10.1016/j.jinorgbio.2012.01.012

Mihajlović-Lalić L, Savić A, Poljarević J, Vučković IM, Mojic M, Bulatović MZ, Maksimovic-Ivanic D, Mijatovic S, Kaluđerović GN, Stošić-Grujičić SD, Miljković Đ, Grgurić-Šipka S, Sabo T. Novel methylene modified cyclohexyl ethylenediamine-N,N '-diacetate ligands and their platinum(IV) complexes. Influence on biological activity. in Journal of Inorganic Biochemistry. 2012;109:40-48.
doi:10.1016/j.jinorgbio.2012.01.012 .

Mihajlović-Lalić, Ljiljana, Savić, Aleksandar, Poljarević, Jelena, Vučković, Ivan M., Mojic, Marija, Bulatović, Mirna Z., Maksimovic-Ivanic, Danijela, Mijatovic, Sanja, Kaluđerović, Goran N., Stošić-Grujičić, Stanislava D., Miljković, Đorđe, Grgurić-Šipka, Sanja, Sabo, Tibor, "Novel methylene modified cyclohexyl ethylenediamine-N,N '-diacetate ligands and their platinum(IV) complexes. Influence on biological activity" in Journal of Inorganic Biochemistry, 109 (2012):40-48,
https://doi.org/10.1016/j.jinorgbio.2012.01.012 . .

TY  - JOUR
AU  - Miljković, Đorđe
AU  - Poljarević, Jelena
AU  - Petkovic, Filip
AU  - Blazevski, Jana
AU  - Momčilović, Miljana
AU  - Nikolic, Ivana
AU  - Saksida, Tamara
AU  - Stošić-Grujičić, Stanislava D.
AU  - Grgurić-Šipka, Sanja
AU  - Sabo, Tibor
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1258
AB  - We have recently reported that a novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoato ligand has a potent cytotoxic effect on glioma, melanoma and fibrosarcoma cell lines. In this work, we investigated the influence of the Pt(IV) compound on immune cells. We determined its effect on the viability of spleen cells and lymph node cells and on their capability to produce interferon (IFN)-gamma and interleukin (IL)-17. Also, we researched the compound's impact on peritoneal macrophages and generation of NO in these cells. Our results show that the complex has limited influence on cell viability of immune cells, but profound inhibitory effect on the production of examined immune mediators. These results are valuable as they show that the novel Pt(IV) complex applied in concentrations which are effective against tumor cells do not affect immune cell viability. Moreover, they also imply that the complex has immunomodulatory properties. (C) 2011 Elsevier Masson SAS. All rights reserved.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N '-di-2-(3-cyclohexyl)propanoato ligand exerts potent immunomodulatory effects
VL  - 47
SP  - 194
EP  - 201
DO  - 10.1016/j.ejmech.2011.10.042
ER  - 

@article{
author = "Miljković, Đorđe and Poljarević, Jelena and Petkovic, Filip and Blazevski, Jana and Momčilović, Miljana and Nikolic, Ivana and Saksida, Tamara and Stošić-Grujičić, Stanislava D. and Grgurić-Šipka, Sanja and Sabo, Tibor",
year = "2012",
abstract = "We have recently reported that a novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoato ligand has a potent cytotoxic effect on glioma, melanoma and fibrosarcoma cell lines. In this work, we investigated the influence of the Pt(IV) compound on immune cells. We determined its effect on the viability of spleen cells and lymph node cells and on their capability to produce interferon (IFN)-gamma and interleukin (IL)-17. Also, we researched the compound's impact on peritoneal macrophages and generation of NO in these cells. Our results show that the complex has limited influence on cell viability of immune cells, but profound inhibitory effect on the production of examined immune mediators. These results are valuable as they show that the novel Pt(IV) complex applied in concentrations which are effective against tumor cells do not affect immune cell viability. Moreover, they also imply that the complex has immunomodulatory properties. (C) 2011 Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N '-di-2-(3-cyclohexyl)propanoato ligand exerts potent immunomodulatory effects",
volume = "47",
pages = "194-201",
doi = "10.1016/j.ejmech.2011.10.042"
}

Miljković, Đ., Poljarević, J., Petkovic, F., Blazevski, J., Momčilović, M., Nikolic, I., Saksida, T., Stošić-Grujičić, S. D., Grgurić-Šipka, S.,& Sabo, T.. (2012). Novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N '-di-2-(3-cyclohexyl)propanoato ligand exerts potent immunomodulatory effects. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 47, 194-201.
https://doi.org/10.1016/j.ejmech.2011.10.042

Miljković Đ, Poljarević J, Petkovic F, Blazevski J, Momčilović M, Nikolic I, Saksida T, Stošić-Grujičić SD, Grgurić-Šipka S, Sabo T. Novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N '-di-2-(3-cyclohexyl)propanoato ligand exerts potent immunomodulatory effects. in European Journal of Medicinal Chemistry. 2012;47:194-201.
doi:10.1016/j.ejmech.2011.10.042 .

Miljković, Đorđe, Poljarević, Jelena, Petkovic, Filip, Blazevski, Jana, Momčilović, Miljana, Nikolic, Ivana, Saksida, Tamara, Stošić-Grujičić, Stanislava D., Grgurić-Šipka, Sanja, Sabo, Tibor, "Novel octahedral Pt(IV) complex with di-n-propyl-(S,S)-ethylenediamine-N,N '-di-2-(3-cyclohexyl)propanoato ligand exerts potent immunomodulatory effects" in European Journal of Medicinal Chemistry, 47 (2012):194-201,
https://doi.org/10.1016/j.ejmech.2011.10.042 . .

TY  - JOUR
AU  - Maksimovic-Ivanic, Danijela
AU  - Mijatovic, Sanja
AU  - Mirkov, Ivana
AU  - Stošić-Grujičić, Stanislava D.
AU  - Miljković, Đorđe
AU  - Sabo, Tibor
AU  - Trajković, Vladimir S.
AU  - Kaluđerović, Goran N.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1551
AB  - Tetrachlorido(O,O'-dibutyl-ethylenediamine-N,N'-di-3-propionate)-platinum(IV) complex, [PtCl4(n-Bu(2)eddp)], was previously found to be effective against fibrosarcoma and glioma cell lines. Here we presented that [PtCl4(n-Bu(2)eddp)] strongly reduced the growth of B16 melanoma cells in vitro. Inhibition of cell viability was accompanied with induction of both necrotic and apoptotic cell death. In addition, [PtCl4(n-Bu(2)eddp)] concealed the expansion of tumors induced in syngeneic C57BI/6 mice without visible signs of nephrotoxicity.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Melanoma tumor inhibition by tetrachlorido(O,O '-dibutyl-ethylenediamine-N,N '-di-3-propionate)platinum(IV) complex: in vitro and in vivo investigations
VL  - 4
IS  - 11
SP  - 1155
EP  - 1159
DO  - 10.1039/c2mt20150j
ER  - 

@article{
author = "Maksimovic-Ivanic, Danijela and Mijatovic, Sanja and Mirkov, Ivana and Stošić-Grujičić, Stanislava D. and Miljković, Đorđe and Sabo, Tibor and Trajković, Vladimir S. and Kaluđerović, Goran N.",
year = "2012",
abstract = "Tetrachlorido(O,O'-dibutyl-ethylenediamine-N,N'-di-3-propionate)-platinum(IV) complex, [PtCl4(n-Bu(2)eddp)], was previously found to be effective against fibrosarcoma and glioma cell lines. Here we presented that [PtCl4(n-Bu(2)eddp)] strongly reduced the growth of B16 melanoma cells in vitro. Inhibition of cell viability was accompanied with induction of both necrotic and apoptotic cell death. In addition, [PtCl4(n-Bu(2)eddp)] concealed the expansion of tumors induced in syngeneic C57BI/6 mice without visible signs of nephrotoxicity.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Melanoma tumor inhibition by tetrachlorido(O,O '-dibutyl-ethylenediamine-N,N '-di-3-propionate)platinum(IV) complex: in vitro and in vivo investigations",
volume = "4",
number = "11",
pages = "1155-1159",
doi = "10.1039/c2mt20150j"
}

Maksimovic-Ivanic, D., Mijatovic, S., Mirkov, I., Stošić-Grujičić, S. D., Miljković, Đ., Sabo, T., Trajković, V. S.,& Kaluđerović, G. N.. (2012). Melanoma tumor inhibition by tetrachlorido(O,O '-dibutyl-ethylenediamine-N,N '-di-3-propionate)platinum(IV) complex: in vitro and in vivo investigations. in Metallomics
Royal Soc Chemistry, Cambridge., 4(11), 1155-1159.
https://doi.org/10.1039/c2mt20150j

Maksimovic-Ivanic D, Mijatovic S, Mirkov I, Stošić-Grujičić SD, Miljković Đ, Sabo T, Trajković VS, Kaluđerović GN. Melanoma tumor inhibition by tetrachlorido(O,O '-dibutyl-ethylenediamine-N,N '-di-3-propionate)platinum(IV) complex: in vitro and in vivo investigations. in Metallomics. 2012;4(11):1155-1159.
doi:10.1039/c2mt20150j .

Maksimovic-Ivanic, Danijela, Mijatovic, Sanja, Mirkov, Ivana, Stošić-Grujičić, Stanislava D., Miljković, Đorđe, Sabo, Tibor, Trajković, Vladimir S., Kaluđerović, Goran N., "Melanoma tumor inhibition by tetrachlorido(O,O '-dibutyl-ethylenediamine-N,N '-di-3-propionate)platinum(IV) complex: in vitro and in vivo investigations" in Metallomics, 4, no. 11 (2012):1155-1159,
https://doi.org/10.1039/c2mt20150j . .

TY  - JOUR
AU  - Markovic, M
AU  - Knezevic, N
AU  - Momčilović, Miljana
AU  - Grgurić-Šipka, Sanja
AU  - Harhaji-Trajkovic, Ljubica
AU  - Trajković, Vladimir S.
AU  - Stojkovic, MM
AU  - Sabo, Tibor
AU  - Miljković, Đorđe
PY  - 2005
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/718
AB  - There has been a continuing effort for the discovery of novel platinum(IV)-based antitumor compounds with better therapeutic performances than cisplatin. In the present work, the anticancer action of recently synthesized Pt(IV)-based complex [Pt(HPxSC)Cl-3] was investigated using rat and human astrocytoma cell lines C6 and U251. [Pt(HPxSC)Cl-3] markedly reduced the number of cultured astrocytoma Cells (IC50, 80 mu M), as determined by crystal violet assay. The Pt(IV) complex induced apoptotic death of tumor cells, as flow cytometry analysis of the propidium iodide-stained cellular DNA revealed approx. 30% of hypodiploid cells in [Pt(HPxSC)Cl-3]-treated astrocytoma cell cultures. On the other hand, [Pt(HPxSC)Cl-3] at 200 mu M did not affect the viability of rat primary astrocytes, unlike the established anticancer drug cisplatin, which displayed high toxicity toward both astrocytoma cells (IC50, 15 mu M) and primary astrocytes (IC50, 20 mu M). Moreover, [Pt(HPxSC)Cl-3] at 100 mu M did not interfere with the ability of rat peritoneal macrophages to produce important antitumor molecules nitric oxide and tumor necrosis factor-a. Finally, we assessed the ability of [Pt(HPxSC)Cl-3] to restrain growth of some bacterial and yeast strains, but it showed rather limited antimicrobial activity. (c) 2005 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - European Journal of Pharmacology
T1  - [Pt(HPxSC)Cl-3], a novel platinum(IV) compound with anticancer properties
VL  - 517
IS  - 1-2
SP  - 28
EP  - 34
DO  - 10.1016/j.ejphar.2005.05.038
ER  - 

@article{
author = "Markovic, M and Knezevic, N and Momčilović, Miljana and Grgurić-Šipka, Sanja and Harhaji-Trajkovic, Ljubica and Trajković, Vladimir S. and Stojkovic, MM and Sabo, Tibor and Miljković, Đorđe",
year = "2005",
abstract = "There has been a continuing effort for the discovery of novel platinum(IV)-based antitumor compounds with better therapeutic performances than cisplatin. In the present work, the anticancer action of recently synthesized Pt(IV)-based complex [Pt(HPxSC)Cl-3] was investigated using rat and human astrocytoma cell lines C6 and U251. [Pt(HPxSC)Cl-3] markedly reduced the number of cultured astrocytoma Cells (IC50, 80 mu M), as determined by crystal violet assay. The Pt(IV) complex induced apoptotic death of tumor cells, as flow cytometry analysis of the propidium iodide-stained cellular DNA revealed approx. 30% of hypodiploid cells in [Pt(HPxSC)Cl-3]-treated astrocytoma cell cultures. On the other hand, [Pt(HPxSC)Cl-3] at 200 mu M did not affect the viability of rat primary astrocytes, unlike the established anticancer drug cisplatin, which displayed high toxicity toward both astrocytoma cells (IC50, 15 mu M) and primary astrocytes (IC50, 20 mu M). Moreover, [Pt(HPxSC)Cl-3] at 100 mu M did not interfere with the ability of rat peritoneal macrophages to produce important antitumor molecules nitric oxide and tumor necrosis factor-a. Finally, we assessed the ability of [Pt(HPxSC)Cl-3] to restrain growth of some bacterial and yeast strains, but it showed rather limited antimicrobial activity. (c) 2005 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "European Journal of Pharmacology",
title = "[Pt(HPxSC)Cl-3], a novel platinum(IV) compound with anticancer properties",
volume = "517",
number = "1-2",
pages = "28-34",
doi = "10.1016/j.ejphar.2005.05.038"
}

Markovic, M., Knezevic, N., Momčilović, M., Grgurić-Šipka, S., Harhaji-Trajkovic, L., Trajković, V. S., Stojkovic, M., Sabo, T.,& Miljković, Đ.. (2005). [Pt(HPxSC)Cl-3], a novel platinum(IV) compound with anticancer properties. in European Journal of Pharmacology
Elsevier Science Bv, Amsterdam., 517(1-2), 28-34.
https://doi.org/10.1016/j.ejphar.2005.05.038

Markovic M, Knezevic N, Momčilović M, Grgurić-Šipka S, Harhaji-Trajkovic L, Trajković VS, Stojkovic M, Sabo T, Miljković Đ. [Pt(HPxSC)Cl-3], a novel platinum(IV) compound with anticancer properties. in European Journal of Pharmacology. 2005;517(1-2):28-34.
doi:10.1016/j.ejphar.2005.05.038 .

Markovic, M, Knezevic, N, Momčilović, Miljana, Grgurić-Šipka, Sanja, Harhaji-Trajkovic, Ljubica, Trajković, Vladimir S., Stojkovic, MM, Sabo, Tibor, Miljković, Đorđe, "[Pt(HPxSC)Cl-3], a novel platinum(IV) compound with anticancer properties" in European Journal of Pharmacology, 517, no. 1-2 (2005):28-34,
https://doi.org/10.1016/j.ejphar.2005.05.038 . .

TY  - JOUR
AU  - Kaludjerovic, GN
AU  - Miljković, Đorđe
AU  - Momčilović, Miljana
AU  - Djinovic, VM
AU  - Stojkovic, MM
AU  - Sabo, Tibor
AU  - Trajković, Vladimir S.
PY  - 2005
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/719
AB  - The anticancer activity of platinum complexes has been known since the discovery of classical Pt(II)-based drug cisplatin. However, Pt(IV) complexes have greater inertness than corresponding Pt(II) complexes, thus allowing the oral administration and reducing the toxicity associated with platinum-based chemotherapy. Here, we describe the in vitro antitumor activity of some novel Pt(IV)-based agents against mouse fibrosarcoma L929 cells and human astrocytoma U251 cells. The cytotoxicity of 2 Pt(IV) complexes with bidentate ethylenediamine-N,N'-di-3-propanoato esters was found to be markedly higher than that of their Pt(II) counterparts and comparable to the antitumor action of cisplatin. In contrast to cisplatin, which caused oxidative stress-independent apoptotic cell death of tumor cells, these Pt(IV) complexes induced oxygen radical-mediated tumor cell necrosis. Importantly, the cytotoxic action of novel Pt(IV) complexes was markedly more rapid than that of cisplatin, indicating their potential usefulness in anticancer therapy. (C) 2005 Wiley-Liss, Inc.
PB  - Wiley, Hoboken
T2  - International Journal of Cancer
T1  - Novel platinum(IV) complexes induce rapid tumor cell death in vitro
VL  - 116
IS  - 3
SP  - 479
EP  - 486
DO  - 10.1002/ijc.21080
ER  - 

@article{
author = "Kaludjerovic, GN and Miljković, Đorđe and Momčilović, Miljana and Djinovic, VM and Stojkovic, MM and Sabo, Tibor and Trajković, Vladimir S.",
year = "2005",
abstract = "The anticancer activity of platinum complexes has been known since the discovery of classical Pt(II)-based drug cisplatin. However, Pt(IV) complexes have greater inertness than corresponding Pt(II) complexes, thus allowing the oral administration and reducing the toxicity associated with platinum-based chemotherapy. Here, we describe the in vitro antitumor activity of some novel Pt(IV)-based agents against mouse fibrosarcoma L929 cells and human astrocytoma U251 cells. The cytotoxicity of 2 Pt(IV) complexes with bidentate ethylenediamine-N,N'-di-3-propanoato esters was found to be markedly higher than that of their Pt(II) counterparts and comparable to the antitumor action of cisplatin. In contrast to cisplatin, which caused oxidative stress-independent apoptotic cell death of tumor cells, these Pt(IV) complexes induced oxygen radical-mediated tumor cell necrosis. Importantly, the cytotoxic action of novel Pt(IV) complexes was markedly more rapid than that of cisplatin, indicating their potential usefulness in anticancer therapy. (C) 2005 Wiley-Liss, Inc.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Cancer",
title = "Novel platinum(IV) complexes induce rapid tumor cell death in vitro",
volume = "116",
number = "3",
pages = "479-486",
doi = "10.1002/ijc.21080"
}

Kaludjerovic, G., Miljković, Đ., Momčilović, M., Djinovic, V., Stojkovic, M., Sabo, T.,& Trajković, V. S.. (2005). Novel platinum(IV) complexes induce rapid tumor cell death in vitro. in International Journal of Cancer
Wiley, Hoboken., 116(3), 479-486.
https://doi.org/10.1002/ijc.21080

Kaludjerovic G, Miljković Đ, Momčilović M, Djinovic V, Stojkovic M, Sabo T, Trajković VS. Novel platinum(IV) complexes induce rapid tumor cell death in vitro. in International Journal of Cancer. 2005;116(3):479-486.
doi:10.1002/ijc.21080 .

Kaludjerovic, GN, Miljković, Đorđe, Momčilović, Miljana, Djinovic, VM, Stojkovic, MM, Sabo, Tibor, Trajković, Vladimir S., "Novel platinum(IV) complexes induce rapid tumor cell death in vitro" in International Journal of Cancer, 116, no. 3 (2005):479-486,
https://doi.org/10.1002/ijc.21080 . .

TY  - JOUR
AU  - Mijatovic, S
AU  - Maksimovic-Ivanic, D
AU  - Radovic, J
AU  - Miljković, Đorđe
AU  - Kaludjerovic, GN
AU  - Sabo, Tibor
AU  - Trajković, Vladimir S.
PY  - 2005
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/733
AB  - The present study describes the ability of an anthraquinone derivative aloe emodin (AE) to reduce the cytotoxic activity of the platinum(II)-based anticancer agent cisplatin toward murine L929 fibrosarcoma and C6 glioma cell lines. The protective effect of AE was demonstrated by MTT and crystal violet assays for cell viability, and involved supression of cisplatin-induced apoptosis and necrosis, as assessed by lactate dehydrogenase release and flow cytometric analysis of DNA fragmentation or phosphatidylserine exposure. Cell-based ELISA and Western blot analysis revealed that AE abolished cisplatin-triggered activation of extracellular signal-regulated kinase (ERK) in tumor cells, while activation of c-Jun N-terminal kinase was not significantly altered. A selective blockade of ERK activation with PD98059 mimicked the protective effect of AE treatment in both tumor cell lines. Moreover, AE failed to protect tumor cells against the ERK-independent toxicity of the Pt(IV)-based complex tetrachloro(O,O-dibutyl-ethylenediamine-N,N'-di-3-propanoate)platinum(IV). Taken together, these data indicate that herbal anthraquinone AE can downregulate the anticancer activity of cisplatin by blocking the activation of ERK in tumor cells.
PB  - Birkhauser Verlag Ag, Basel
T2  - Cellular and Molecular Life Sciences / CMLS
T1  - Aloe emodin decreases the ERK-dependent anticancer activity of cisplatin
VL  - 62
IS  - 11
SP  - 1275
EP  - 1282
DO  - 10.1007/s00018-005-5041-3
ER  - 

@article{
author = "Mijatovic, S and Maksimovic-Ivanic, D and Radovic, J and Miljković, Đorđe and Kaludjerovic, GN and Sabo, Tibor and Trajković, Vladimir S.",
year = "2005",
abstract = "The present study describes the ability of an anthraquinone derivative aloe emodin (AE) to reduce the cytotoxic activity of the platinum(II)-based anticancer agent cisplatin toward murine L929 fibrosarcoma and C6 glioma cell lines. The protective effect of AE was demonstrated by MTT and crystal violet assays for cell viability, and involved supression of cisplatin-induced apoptosis and necrosis, as assessed by lactate dehydrogenase release and flow cytometric analysis of DNA fragmentation or phosphatidylserine exposure. Cell-based ELISA and Western blot analysis revealed that AE abolished cisplatin-triggered activation of extracellular signal-regulated kinase (ERK) in tumor cells, while activation of c-Jun N-terminal kinase was not significantly altered. A selective blockade of ERK activation with PD98059 mimicked the protective effect of AE treatment in both tumor cell lines. Moreover, AE failed to protect tumor cells against the ERK-independent toxicity of the Pt(IV)-based complex tetrachloro(O,O-dibutyl-ethylenediamine-N,N'-di-3-propanoate)platinum(IV). Taken together, these data indicate that herbal anthraquinone AE can downregulate the anticancer activity of cisplatin by blocking the activation of ERK in tumor cells.",
publisher = "Birkhauser Verlag Ag, Basel",
journal = "Cellular and Molecular Life Sciences / CMLS",
title = "Aloe emodin decreases the ERK-dependent anticancer activity of cisplatin",
volume = "62",
number = "11",
pages = "1275-1282",
doi = "10.1007/s00018-005-5041-3"
}

Mijatovic, S., Maksimovic-Ivanic, D., Radovic, J., Miljković, Đ., Kaludjerovic, G., Sabo, T.,& Trajković, V. S.. (2005). Aloe emodin decreases the ERK-dependent anticancer activity of cisplatin. in Cellular and Molecular Life Sciences / CMLS
Birkhauser Verlag Ag, Basel., 62(11), 1275-1282.
https://doi.org/10.1007/s00018-005-5041-3

Mijatovic S, Maksimovic-Ivanic D, Radovic J, Miljković Đ, Kaludjerovic G, Sabo T, Trajković VS. Aloe emodin decreases the ERK-dependent anticancer activity of cisplatin. in Cellular and Molecular Life Sciences / CMLS. 2005;62(11):1275-1282.
doi:10.1007/s00018-005-5041-3 .

Mijatovic, S, Maksimovic-Ivanic, D, Radovic, J, Miljković, Đorđe, Kaludjerovic, GN, Sabo, Tibor, Trajković, Vladimir S., "Aloe emodin decreases the ERK-dependent anticancer activity of cisplatin" in Cellular and Molecular Life Sciences / CMLS, 62, no. 11 (2005):1275-1282,
https://doi.org/10.1007/s00018-005-5041-3 . .

TY  - JOUR
AU  - Djinovic, V
AU  - Momčilović, Miljana
AU  - Grgurić-Šipka, Sanja
AU  - Trajković, Vladimir S.
AU  - Stojkovic, MM
AU  - Miljković, Đorđe
AU  - Sabo, Tibor
PY  - 2004
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/672
AB  - A novel class of ruthenium (111) complexes of formulas K[Ru(sar)(2)Cl-2] - 1/2HO and K-2[Ru(dmgly)Cl-4] . 1/2H(2)O, containing biden2 tate chelates N-methylglycine (sarcosine, sar) or N,N-dimethylglycine (dnigly) and additional chloro ligands were synthesized. The complexes have been obtained by direct reaction of ruthenium(III) chloride with corresponding bidentate ligand followed by addition of base (KOH). These new complexes were characterized by elemental analysis, IR and electronic absorption spectroscopy. As astrocytomas, the most common of all brain tumors, are still very difficult to treat, we examined the influence of newly synthesized ruthenium-based complexes, as well as the earlier synthesized analogue platinum(IV) complexes [Pt(dmglY)(2)Cl-2], [Pt(sar)(2)Br-2] and [Pt(dmgly)(2)Br-2] on rat astrocytoma C6 cells in vitro. Among these complexes only K-2[Ru(dmglY)Cl-4].2H(2)O and [Pt(dMgIY)(2)Br-2] markedly inhibited the viability of non-confluent C6 cells. Furthermore, only complex K-2[Ru(dmgly)Cl4] .2H20 was able to reduce viability in confluent C6 cultures. Importantly, this complex was not toxic to primary rat astrocytes or macrophages. Having in mind that appropriate chemotherapy should be effective against tumor cells without harming normal tissues, complex K,[Ru(dmglY)Cl-4] - 2H(2)O could be a promising agent for developing therapeutics against astrocytomas. (C) 2004 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - Novel ruthenium complex K-2[Ru(dmgly)Cl-4] center dot 2H(2)O is toxic to C6 astrocytoma cell line, but not to primary rat astrocytes
VL  - 98
IS  - 12
SP  - 2168
EP  - 2173
DO  - 10.1016/j.jinorgbio.2004.10.007
ER  - 

@article{
author = "Djinovic, V and Momčilović, Miljana and Grgurić-Šipka, Sanja and Trajković, Vladimir S. and Stojkovic, MM and Miljković, Đorđe and Sabo, Tibor",
year = "2004",
abstract = "A novel class of ruthenium (111) complexes of formulas K[Ru(sar)(2)Cl-2] - 1/2HO and K-2[Ru(dmgly)Cl-4] . 1/2H(2)O, containing biden2 tate chelates N-methylglycine (sarcosine, sar) or N,N-dimethylglycine (dnigly) and additional chloro ligands were synthesized. The complexes have been obtained by direct reaction of ruthenium(III) chloride with corresponding bidentate ligand followed by addition of base (KOH). These new complexes were characterized by elemental analysis, IR and electronic absorption spectroscopy. As astrocytomas, the most common of all brain tumors, are still very difficult to treat, we examined the influence of newly synthesized ruthenium-based complexes, as well as the earlier synthesized analogue platinum(IV) complexes [Pt(dmglY)(2)Cl-2], [Pt(sar)(2)Br-2] and [Pt(dmgly)(2)Br-2] on rat astrocytoma C6 cells in vitro. Among these complexes only K-2[Ru(dmglY)Cl-4].2H(2)O and [Pt(dMgIY)(2)Br-2] markedly inhibited the viability of non-confluent C6 cells. Furthermore, only complex K-2[Ru(dmgly)Cl4] .2H20 was able to reduce viability in confluent C6 cultures. Importantly, this complex was not toxic to primary rat astrocytes or macrophages. Having in mind that appropriate chemotherapy should be effective against tumor cells without harming normal tissues, complex K,[Ru(dmglY)Cl-4] - 2H(2)O could be a promising agent for developing therapeutics against astrocytomas. (C) 2004 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "Novel ruthenium complex K-2[Ru(dmgly)Cl-4] center dot 2H(2)O is toxic to C6 astrocytoma cell line, but not to primary rat astrocytes",
volume = "98",
number = "12",
pages = "2168-2173",
doi = "10.1016/j.jinorgbio.2004.10.007"
}

Djinovic, V., Momčilović, M., Grgurić-Šipka, S., Trajković, V. S., Stojkovic, M., Miljković, Đ.,& Sabo, T.. (2004). Novel ruthenium complex K-2[Ru(dmgly)Cl-4] center dot 2H(2)O is toxic to C6 astrocytoma cell line, but not to primary rat astrocytes. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 98(12), 2168-2173.
https://doi.org/10.1016/j.jinorgbio.2004.10.007

Djinovic V, Momčilović M, Grgurić-Šipka S, Trajković VS, Stojkovic M, Miljković Đ, Sabo T. Novel ruthenium complex K-2[Ru(dmgly)Cl-4] center dot 2H(2)O is toxic to C6 astrocytoma cell line, but not to primary rat astrocytes. in Journal of Inorganic Biochemistry. 2004;98(12):2168-2173.
doi:10.1016/j.jinorgbio.2004.10.007 .

Djinovic, V, Momčilović, Miljana, Grgurić-Šipka, Sanja, Trajković, Vladimir S., Stojkovic, MM, Miljković, Đorđe, Sabo, Tibor, "Novel ruthenium complex K-2[Ru(dmgly)Cl-4] center dot 2H(2)O is toxic to C6 astrocytoma cell line, but not to primary rat astrocytes" in Journal of Inorganic Biochemistry, 98, no. 12 (2004):2168-2173,
https://doi.org/10.1016/j.jinorgbio.2004.10.007 . .