TY  - JOUR
AU  - Gođevac, Dejan
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Anđelković, Boban D.
AU  - Jovanović, Živko S.
AU  - Rakić, Tamara
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.3151
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5417
AB  - Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.
PB  - Wiley
T2  - Phytochemical Analysis
T2  - Phytochemical Analysis
T1  - Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae
VL  - 33
IS  - 6
SP  - 961
EP  - 970
DO  - 10.1002/pca.3151
ER  - 

@article{
author = "Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Anđelković, Boban D. and Jovanović, Živko S. and Rakić, Tamara",
year = "2022",
abstract = "Introduction Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated. Objective To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process Methods Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates. Results Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples. Conclusion Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.",
publisher = "Wiley",
journal = "Phytochemical Analysis, Phytochemical Analysis",
title = "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae",
volume = "33",
number = "6",
pages = "961-970",
doi = "10.1002/pca.3151"
}

Gođevac, D., Ivanović, S., Simić, K., Anđelković, B. D., Jovanović, Ž. S.,& Rakić, T.. (2022). Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis
Wiley., 33(6), 961-970.
https://doi.org/10.1002/pca.3151

Gođevac D, Ivanović S, Simić K, Anđelković BD, Jovanović ŽS, Rakić T. Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis. 2022;33(6):961-970.
doi:10.1002/pca.3151 .

Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Anđelković, Boban D., Jovanović, Živko S., Rakić, Tamara, "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae" in Phytochemical Analysis, 33, no. 6 (2022):961-970,
https://doi.org/10.1002/pca.3151 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko S.
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beškoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4300
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-γ. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Soković Bajić, Svetlana and Jovanović, Živko S. and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beškoski, Vladimir and Samardžić, Jelena",
year = "2020",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-γ. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}

Stanisavljević, N. S., Soković Bajić, S., Jovanović, Ž. S., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beškoski, V.,& Samardžić, J.. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology
Frontiers., 11.
https://doi.org/10.3389/fmicb.2020.601616

Stanisavljević NS, Soković Bajić S, Jovanović ŽS, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beškoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology. 2020;11.
doi:10.3389/fmicb.2020.601616 .

Stanisavljević, Nemanja S., Soković Bajić, Svetlana, Jovanović, Živko S., Matić, Ivana, Tolinački, Maja, Popović, Dušanka, Popović, Nikola, Terzić-Vidojević, Amarela, Golić, Nataša, Beškoski, Vladimir, Samardžić, Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" in Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 . .

TY  - JOUR
AU  - Mihailović, Nevena
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Stanisavljević, Nemanja S.
AU  - Jovanović, Živko S.
AU  - Trifunović, Snežana S.
AU  - Joksović, Ljubinka
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2398
AB  - Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids
VL  - 7
IS  - 14
SP  - 8550
EP  - 8560
DO  - 10.1039/c6ra28787e
ER  - 

@article{
author = "Mihailović, Nevena and Marković, Violeta and Matić, Ivana Z. and Stanisavljević, Nemanja S. and Jovanović, Živko S. and Trifunović, Snežana S. and Joksović, Ljubinka",
year = "2017",
abstract = "Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids",
volume = "7",
number = "14",
pages = "8550-8560",
doi = "10.1039/c6ra28787e"
}

Mihailović, N., Marković, V., Matić, I. Z., Stanisavljević, N. S., Jovanović, Ž. S., Trifunović, S. S.,& Joksović, L.. (2017). Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances
Royal Soc Chemistry, Cambridge., 7(14), 8550-8560.
https://doi.org/10.1039/c6ra28787e

Mihailović N, Marković V, Matić IZ, Stanisavljević NS, Jovanović ŽS, Trifunović SS, Joksović L. Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances. 2017;7(14):8550-8560.
doi:10.1039/c6ra28787e .

Mihailović, Nevena, Marković, Violeta, Matić, Ivana Z., Stanisavljević, Nemanja S., Jovanović, Živko S., Trifunović, Snežana S., Joksović, Ljubinka, "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids" in RSC Advances, 7, no. 14 (2017):8550-8560,
https://doi.org/10.1039/c6ra28787e . .

TY  - DATA
AU  - Mihailović, Nevena
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Stanisavljević, Nemanja S.
AU  - Jovanović, Živko S.
AU  - Trifunović, Snežana S.
AU  - Joksović, Ljubinka
PY  - 2017
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2980
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Supplementary data for article: Mihailovic, N.; Marković, V.; Matić, I. Z.; Stanisavljević, N. S.; Jovanovic, Z. S.; Trifunović, S. S.; Joksović, L. Synthesis and Antioxidant Activity of 1,3,4-Oxadiazoles and Their Diacylhydrazine Precursors Derived from Phenolic Acids. RSC Advances 2017, 7 (14), 8550–8560. https://doi.org/10.1039/c6ra28787e
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2980
ER  - 

@misc{
author = "Mihailović, Nevena and Marković, Violeta and Matić, Ivana Z. and Stanisavljević, Nemanja S. and Jovanović, Živko S. and Trifunović, Snežana S. and Joksović, Ljubinka",
year = "2017, 2017",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Supplementary data for article: Mihailovic, N.; Marković, V.; Matić, I. Z.; Stanisavljević, N. S.; Jovanovic, Z. S.; Trifunović, S. S.; Joksović, L. Synthesis and Antioxidant Activity of 1,3,4-Oxadiazoles and Their Diacylhydrazine Precursors Derived from Phenolic Acids. RSC Advances 2017, 7 (14), 8550–8560. https://doi.org/10.1039/c6ra28787e",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2980"
}

Mihailović, N., Marković, V., Matić, I. Z., Stanisavljević, N. S., Jovanović, Ž. S., Trifunović, S. S.,& Joksović, L.. (2017). Supplementary data for article: Mihailovic, N.; Marković, V.; Matić, I. Z.; Stanisavljević, N. S.; Jovanovic, Z. S.; Trifunović, S. S.; Joksović, L. Synthesis and Antioxidant Activity of 1,3,4-Oxadiazoles and Their Diacylhydrazine Precursors Derived from Phenolic Acids. RSC Advances 2017, 7 (14), 8550–8560. https://doi.org/10.1039/c6ra28787e. in RSC Advances
Royal Soc Chemistry, Cambridge..
https://hdl.handle.net/21.15107/rcub_cherry_2980

Mihailović N, Marković V, Matić IZ, Stanisavljević NS, Jovanović ŽS, Trifunović SS, Joksović L. Supplementary data for article: Mihailovic, N.; Marković, V.; Matić, I. Z.; Stanisavljević, N. S.; Jovanovic, Z. S.; Trifunović, S. S.; Joksović, L. Synthesis and Antioxidant Activity of 1,3,4-Oxadiazoles and Their Diacylhydrazine Precursors Derived from Phenolic Acids. RSC Advances 2017, 7 (14), 8550–8560. https://doi.org/10.1039/c6ra28787e. in RSC Advances. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_2980 .

Mihailović, Nevena, Marković, Violeta, Matić, Ivana Z., Stanisavljević, Nemanja S., Jovanović, Živko S., Trifunović, Snežana S., Joksović, Ljubinka, "Supplementary data for article: Mihailovic, N.; Marković, V.; Matić, I. Z.; Stanisavljević, N. S.; Jovanovic, Z. S.; Trifunović, S. S.; Joksović, L. Synthesis and Antioxidant Activity of 1,3,4-Oxadiazoles and Their Diacylhydrazine Precursors Derived from Phenolic Acids. RSC Advances 2017, 7 (14), 8550–8560. https://doi.org/10.1039/c6ra28787e" in RSC Advances (2017),
https://hdl.handle.net/21.15107/rcub_cherry_2980 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Ilić, Marija D.
AU  - Matić, Ivana Z.
AU  - Jovanović, Živko S.
AU  - Čupić, Tihomir
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2291
AB  - To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer. An International Journal
T1  - Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities
VL  - 68
IS  - 6
SP  - 988
EP  - 1000
DO  - 10.1080/01635581.2016.1190019
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj.",
year = "2016",
abstract = "To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer. An International Journal",
title = "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities",
volume = "68",
number = "6",
pages = "988-1000",
doi = "10.1080/01635581.2016.1190019"
}

Stanisavljević, N. S., Ilić, M. D., Matić, I. Z., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M.,& Tešić, Ž. Lj.. (2016). Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer. An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 68(6), 988-1000.
https://doi.org/10.1080/01635581.2016.1190019

Stanisavljević NS, Ilić MD, Matić IZ, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL. Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer. An International Journal. 2016;68(6):988-1000.
doi:10.1080/01635581.2016.1190019 .

Stanisavljević, Nemanja S., Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities" in Nutrition and Cancer. An International Journal, 68, no. 6 (2016):988-1000,
https://doi.org/10.1080/01635581.2016.1190019 . .

TY  - DATA
AU  - Stanisavljević, Nemanja S.
AU  - Ilić, Marija D.
AU  - Matić, Ivana Z.
AU  - Jovanović, Živko S.
AU  - Čupić, Tihomir
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3534
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer. An International Journal
T1  - Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3534
ER  - 

@misc{
author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj.",
year = "2016",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer. An International Journal",
title = "Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3534"
}

Stanisavljević, N. S., Ilić, M. D., Matić, I. Z., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M.,& Tešić, Ž. Lj.. (2016). Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.. in Nutrition and Cancer. An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon..
https://hdl.handle.net/21.15107/rcub_cherry_3534

Stanisavljević NS, Ilić MD, Matić IZ, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL. Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.. in Nutrition and Cancer. An International Journal. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3534 .

Stanisavljević, Nemanja S., Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., "Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019." in Nutrition and Cancer. An International Journal (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3534 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Vukotić, Goran N.
AU  - Pastor, Ferenc
AU  - Sužnjević, Desanka
AU  - Jovanović, Živko S.
AU  - Strahinic, Ivana D.
AU  - Fira, Dorde A.
AU  - Radovic, Svetlana S.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1961
AB  - Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria
VL  - 67
IS  - 3
SP  - 1033
EP  - 1042
DO  - 10.2298/ABS150130066S
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Vukotić, Goran N. and Pastor, Ferenc and Sužnjević, Desanka and Jovanović, Živko S. and Strahinic, Ivana D. and Fira, Dorde A. and Radovic, Svetlana S.",
year = "2015",
abstract = "Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria",
volume = "67",
number = "3",
pages = "1033-1042",
doi = "10.2298/ABS150130066S"
}

Stanisavljević, N. S., Vukotić, G. N., Pastor, F., Sužnjević, D., Jovanović, Ž. S., Strahinic, I. D., Fira, D. A.,& Radovic, S. S.. (2015). Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 67(3), 1033-1042.
https://doi.org/10.2298/ABS150130066S

Stanisavljević NS, Vukotić GN, Pastor F, Sužnjević D, Jovanović ŽS, Strahinic ID, Fira DA, Radovic SS. Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria. in Archives of biological sciences. 2015;67(3):1033-1042.
doi:10.2298/ABS150130066S .

Stanisavljević, Nemanja S., Vukotić, Goran N., Pastor, Ferenc, Sužnjević, Desanka, Jovanović, Živko S., Strahinic, Ivana D., Fira, Dorde A., Radovic, Svetlana S., "Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria" in Archives of biological sciences, 67, no. 3 (2015):1033-1042,
https://doi.org/10.2298/ABS150130066S . .

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Ilić, Marija D.
AU  - Jovanović, Živko S.
AU  - Čupić, Tihomir
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
AU  - Radovic, Svetlana S.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2013
AB  - The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity
VL  - 67
IS  - 3
SP  - 829
EP  - 840
DO  - 10.2298/ABS141204042S
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj. and Radovic, Svetlana S.",
year = "2015",
abstract = "The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity",
volume = "67",
number = "3",
pages = "829-840",
doi = "10.2298/ABS141204042S"
}

Stanisavljević, N. S., Ilić, M. D., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M., Tešić, Ž. Lj.,& Radovic, S. S.. (2015). Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 67(3), 829-840.
https://doi.org/10.2298/ABS141204042S

Stanisavljević NS, Ilić MD, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL, Radovic SS. Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity. in Archives of biological sciences. 2015;67(3):829-840.
doi:10.2298/ABS141204042S .

Stanisavljević, Nemanja S., Ilić, Marija D., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., Radovic, Svetlana S., "Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity" in Archives of biological sciences, 67, no. 3 (2015):829-840,
https://doi.org/10.2298/ABS141204042S . .