Protić, Sara

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  • Protić, Sara (2)
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Author's Bibliography

Kloniranje proteaze slične papainu PL pro SARS-CoV-2 u E.coli, heterologna ekspresija, prečišćavanje i razvoj inhibitornog enzimskog eseja za pretragu potencijalnih lekova

Protić, Sara

(2023)

TY  - THES
AU  - Protić, Sara
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5871
AB  - Pandemija izazvana virusom SARS-CoV-2 se pojavila krajem decembra 2019. godine u Vuhanu
u Kini, kada su prvi put zapažena teška respiratorna oboljenja. Od tada se virus brzo proširio u
Kini, ali i u drugim državama i izazvao veliku globalnu zabrinutost. U Srbiji je polovinom marta
2020. nakon registrovanja prvih slučajeva obolelih i proglašenja pandemije od strane Svetske
zdravstvene organizacije uvedeno vanredno stanje. Novi virus SARS-CoV-2 je dobio ime zbog
svoje strukturne sličnosti sa korona virusima koji izazivaju teške respiratorne simptome.
T1  - Kloniranje proteaze slične papainu PL pro SARS-CoV-2 u E.coli, heterologna ekspresija, prečišćavanje i razvoj inhibitornog enzimskog eseja za pretragu potencijalnih lekova
SP  - 2
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5871
ER  - 
@misc{
author = "Protić, Sara",
year = "2023",
abstract = "Pandemija izazvana virusom SARS-CoV-2 se pojavila krajem decembra 2019. godine u Vuhanu
u Kini, kada su prvi put zapažena teška respiratorna oboljenja. Od tada se virus brzo proširio u
Kini, ali i u drugim državama i izazvao veliku globalnu zabrinutost. U Srbiji je polovinom marta
2020. nakon registrovanja prvih slučajeva obolelih i proglašenja pandemije od strane Svetske
zdravstvene organizacije uvedeno vanredno stanje. Novi virus SARS-CoV-2 je dobio ime zbog
svoje strukturne sličnosti sa korona virusima koji izazivaju teške respiratorne simptome.",
title = "Kloniranje proteaze slične papainu PL pro SARS-CoV-2 u E.coli, heterologna ekspresija, prečišćavanje i razvoj inhibitornog enzimskog eseja za pretragu potencijalnih lekova",
pages = "2-35",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5871"
}
Protić, S.. (2023). Kloniranje proteaze slične papainu PL pro SARS-CoV-2 u E.coli, heterologna ekspresija, prečišćavanje i razvoj inhibitornog enzimskog eseja za pretragu potencijalnih lekova. , 2-35.
https://hdl.handle.net/21.15107/rcub_cherry_5871
Protić S. Kloniranje proteaze slične papainu PL pro SARS-CoV-2 u E.coli, heterologna ekspresija, prečišćavanje i razvoj inhibitornog enzimskog eseja za pretragu potencijalnih lekova. 2023;:2-35.
https://hdl.handle.net/21.15107/rcub_cherry_5871 .
Protić, Sara, "Kloniranje proteaze slične papainu PL pro SARS-CoV-2 u E.coli, heterologna ekspresija, prečišćavanje i razvoj inhibitornog enzimskog eseja za pretragu potencijalnih lekova" (2023):2-35,
https://hdl.handle.net/21.15107/rcub_cherry_5871 .

In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D

Glišić, Sanja; Prodanović, Radivoje; Paessler, Slobodan; Perović, Vladimir; Milićević, Jelena; Prodanović, Olivera; Senčanski, Milan ; Kaličanin, Nevena; Protić, Sara

(MDPI, 2023)

TY  - JOUR
AU  - Glišić, Sanja
AU  - Prodanović, Radivoje
AU  - Paessler, Slobodan
AU  - Perović, Vladimir
AU  - Milićević, Jelena
AU  - Prodanović, Olivera
AU  - Senčanski, Milan 
AU  - Kaličanin, Nevena
AU  - Protić, Sara
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5884
AB  - Finding an effective drug to prevent or treat COVID-19 is of utmost importance in tcurrent pandemic. Since developing a new treatment takes a significant amount of time, drug repurposing can be an effective option for achieving a rapid response. This study used a combined in silico virtual screening protocol for candidate SARS-CoV-2 PLpro inhibitors. The Drugbank database was searched first, using the Informational Spectrum Method for Small Molecules, followed by molecular docking. Gramicidin D was selected as a peptide drug, showing the best in silico interaction profile with PLpro. After the expression and purification of PLpro, gramicidin D was screened for protease inhibition in vitro and was found to be active against PLpro. The current study’s findings are significant because it is critical to identify COVID-19 therapies that are efficient, affordable, and have a favorable safety profile.
PB  - MDPI
T2  - Int. J. Mol. Sci.
T1  - In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D
VL  - 24
IS  - 3
SP  - 1955
DO  - 10.3390/ijms24031955
ER  - 
@article{
author = "Glišić, Sanja and Prodanović, Radivoje and Paessler, Slobodan and Perović, Vladimir and Milićević, Jelena and Prodanović, Olivera and Senčanski, Milan  and Kaličanin, Nevena and Protić, Sara",
year = "2023",
abstract = "Finding an effective drug to prevent or treat COVID-19 is of utmost importance in tcurrent pandemic. Since developing a new treatment takes a significant amount of time, drug repurposing can be an effective option for achieving a rapid response. This study used a combined in silico virtual screening protocol for candidate SARS-CoV-2 PLpro inhibitors. The Drugbank database was searched first, using the Informational Spectrum Method for Small Molecules, followed by molecular docking. Gramicidin D was selected as a peptide drug, showing the best in silico interaction profile with PLpro. After the expression and purification of PLpro, gramicidin D was screened for protease inhibition in vitro and was found to be active against PLpro. The current study’s findings are significant because it is critical to identify COVID-19 therapies that are efficient, affordable, and have a favorable safety profile.",
publisher = "MDPI",
journal = "Int. J. Mol. Sci.",
title = "In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D",
volume = "24",
number = "3",
pages = "1955",
doi = "10.3390/ijms24031955"
}
Glišić, S., Prodanović, R., Paessler, S., Perović, V., Milićević, J., Prodanović, O., Senčanski, M., Kaličanin, N.,& Protić, S.. (2023). In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D. in Int. J. Mol. Sci.
MDPI., 24(3), 1955.
https://doi.org/10.3390/ijms24031955
Glišić S, Prodanović R, Paessler S, Perović V, Milićević J, Prodanović O, Senčanski M, Kaličanin N, Protić S. In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D. in Int. J. Mol. Sci.. 2023;24(3):1955.
doi:10.3390/ijms24031955 .
Glišić, Sanja, Prodanović, Radivoje, Paessler, Slobodan, Perović, Vladimir, Milićević, Jelena, Prodanović, Olivera, Senčanski, Milan , Kaličanin, Nevena, Protić, Sara, "In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D" in Int. J. Mol. Sci., 24, no. 3 (2023):1955,
https://doi.org/10.3390/ijms24031955 . .
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