TY  - CONF
AU  - Lončarević, B.
AU  - Lješević, Marija
AU  - Marković, M.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Anđelković, I.
AU  - Beškoski, Vladimir
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4929
AB  - Copper is one of the leading metal pollutants in the water, which can cause adverse effects when present in high concentrations. The Daphnia magna is a model organism usually used for the determination of ecotoxicological effects of various compounds since it is highly sensitive to toxic compounds [1]. The aim of this work was to investigate the potential application of microbial extracellular polysaccharides (EPS), levan and pullulan, as agents for reducing the copper toxicity to D. magna. The protective effects of EPS were estimated based on the accumulation of copper in the D. magna cells. Levan is a branched fructane EPS [2] and the one used in this study was produced by Bacillus licheniformis NS032. Pullulan, a linear glucan EPS [3], was produced by Aureobasidium pullulans CH-1. The D. magna were exposed to 50 µg/dm3  of Cu (II) or a combination with 50 mg/dm3  and 100 mg/dm3  of levan or pullulan for 48h in the acute test. Additionally, the prolonged test was performed, where the daphnia were exposed to a 10 µg/dm3  of Cu (II) with or without 50 mg/ dm3  of levan or pullulan for 5 days. After the exposure period, the samples were digested and the accumulation of copper in D. magna was analysed using the iCAP Qc ICP-MS (Thermo Scientific, United Kingdom). The results showed that animals exposed to Cu (II) only, accumulated Cu (II) in a greater amount after the prolonged test compared to the acute one, despite the lower concentration. The treatment with EPS during the acute test increased the copper accumulation for both EPS concentrations tested, whereas during the prolonged exposure test, the Cu (II) accumulation was inhibited. Considering that protective effects of levan and pullulan were observed only with lower copper concentrations and 5 days of exposure, additional experiments are necessary to determine the mechanism of EPS action in order to confirm their possible use as protective agents.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna
SP  - 159
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4929
ER  - 

@conference{
author = "Lončarević, B. and Lješević, Marija and Marković, M. and Gojgić-Cvijović, Gordana D. and Anđelković, I. and Beškoski, Vladimir",
year = "2021",
abstract = "Copper is one of the leading metal pollutants in the water, which can cause adverse effects when present in high concentrations. The Daphnia magna is a model organism usually used for the determination of ecotoxicological effects of various compounds since it is highly sensitive to toxic compounds [1]. The aim of this work was to investigate the potential application of microbial extracellular polysaccharides (EPS), levan and pullulan, as agents for reducing the copper toxicity to D. magna. The protective effects of EPS were estimated based on the accumulation of copper in the D. magna cells. Levan is a branched fructane EPS [2] and the one used in this study was produced by Bacillus licheniformis NS032. Pullulan, a linear glucan EPS [3], was produced by Aureobasidium pullulans CH-1. The D. magna were exposed to 50 µg/dm3  of Cu (II) or a combination with 50 mg/dm3  and 100 mg/dm3  of levan or pullulan for 48h in the acute test. Additionally, the prolonged test was performed, where the daphnia were exposed to a 10 µg/dm3  of Cu (II) with or without 50 mg/ dm3  of levan or pullulan for 5 days. After the exposure period, the samples were digested and the accumulation of copper in D. magna was analysed using the iCAP Qc ICP-MS (Thermo Scientific, United Kingdom). The results showed that animals exposed to Cu (II) only, accumulated Cu (II) in a greater amount after the prolonged test compared to the acute one, despite the lower concentration. The treatment with EPS during the acute test increased the copper accumulation for both EPS concentrations tested, whereas during the prolonged exposure test, the Cu (II) accumulation was inhibited. Considering that protective effects of levan and pullulan were observed only with lower copper concentrations and 5 days of exposure, additional experiments are necessary to determine the mechanism of EPS action in order to confirm their possible use as protective agents.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna",
pages = "159-159",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4929"
}

Lončarević, B., Lješević, M., Marković, M., Gojgić-Cvijović, G. D., Anđelković, I.,& Beškoski, V.. (2021). The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 159-159.
https://hdl.handle.net/21.15107/rcub_cherry_4929

Lončarević B, Lješević M, Marković M, Gojgić-Cvijović GD, Anđelković I, Beškoski V. The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:159-159.
https://hdl.handle.net/21.15107/rcub_cherry_4929 .

Lončarević, B., Lješević, Marija, Marković, M., Gojgić-Cvijović, Gordana D., Anđelković, I., Beškoski, Vladimir, "The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):159-159,
https://hdl.handle.net/21.15107/rcub_cherry_4929 .

TY  - JOUR
AU  - Kekez, B. D.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Stefanović Kojić, Jovana R.
AU  - Marković, M. D.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1670
AB  - In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm.
PB  - Humana Press Inc, Totowa
T2  - Applied Biochemistry and Biotechnology
T1  - High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source
VL  - 175
IS  - 6
SP  - 3068
EP  - 3083
DO  - 10.1007/s12010-015-1475-8
ER  - 

@article{
author = "Kekez, B. D. and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Stefanović Kojić, Jovana R. and Marković, M. D. and Beškoski, Vladimir and Vrvić, Miroslav M.",
year = "2015",
abstract = "In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm.",
publisher = "Humana Press Inc, Totowa",
journal = "Applied Biochemistry and Biotechnology",
title = "High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source",
volume = "175",
number = "6",
pages = "3068-3083",
doi = "10.1007/s12010-015-1475-8"
}

Kekez, B. D., Gojgić-Cvijović, G. D., Jakovljević, D. M., Stefanović Kojić, J. R., Marković, M. D., Beškoski, V.,& Vrvić, M. M.. (2015). High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. in Applied Biochemistry and Biotechnology
Humana Press Inc, Totowa., 175(6), 3068-3083.
https://doi.org/10.1007/s12010-015-1475-8

Kekez BD, Gojgić-Cvijović GD, Jakovljević DM, Stefanović Kojić JR, Marković MD, Beškoski V, Vrvić MM. High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. in Applied Biochemistry and Biotechnology. 2015;175(6):3068-3083.
doi:10.1007/s12010-015-1475-8 .

Kekez, B. D., Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Stefanović Kojić, Jovana R., Marković, M. D., Beškoski, Vladimir, Vrvić, Miroslav M., "High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source" in Applied Biochemistry and Biotechnology, 175, no. 6 (2015):3068-3083,
https://doi.org/10.1007/s12010-015-1475-8 . .

TY  - DATA
AU  - Kekez, B. D.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Stefanović Kojić, Jovana R.
AU  - Marković, M. D.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3376
PB  - Humana Press Inc, Totowa
T2  - Applied Biochemistry and Biotechnology
T1  - Supplementary data for article: Kekez, B. D.; Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Stefanovic Kojic, J. R.; Markovic, M. D.; Beskoski, V. P.; Vrvic, M. M. High Levan Production by Bacillus Licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. Applied Biochemistry and Biotechnology 2015, 175 (6), 3068–3083. https://doi.org/10.1007/s12010-015-1475-8
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3376
ER  - 

@misc{
author = "Kekez, B. D. and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Stefanović Kojić, Jovana R. and Marković, M. D. and Beškoski, Vladimir and Vrvić, Miroslav M.",
year = "2015",
publisher = "Humana Press Inc, Totowa",
journal = "Applied Biochemistry and Biotechnology",
title = "Supplementary data for article: Kekez, B. D.; Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Stefanovic Kojic, J. R.; Markovic, M. D.; Beskoski, V. P.; Vrvic, M. M. High Levan Production by Bacillus Licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. Applied Biochemistry and Biotechnology 2015, 175 (6), 3068–3083. https://doi.org/10.1007/s12010-015-1475-8",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3376"
}

Kekez, B. D., Gojgić-Cvijović, G. D., Jakovljević, D. M., Stefanović Kojić, J. R., Marković, M. D., Beškoski, V.,& Vrvić, M. M.. (2015). Supplementary data for article: Kekez, B. D.; Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Stefanovic Kojic, J. R.; Markovic, M. D.; Beskoski, V. P.; Vrvic, M. M. High Levan Production by Bacillus Licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. Applied Biochemistry and Biotechnology 2015, 175 (6), 3068–3083. https://doi.org/10.1007/s12010-015-1475-8. in Applied Biochemistry and Biotechnology
Humana Press Inc, Totowa..
https://hdl.handle.net/21.15107/rcub_cherry_3376

Kekez BD, Gojgić-Cvijović GD, Jakovljević DM, Stefanović Kojić JR, Marković MD, Beškoski V, Vrvić MM. Supplementary data for article: Kekez, B. D.; Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Stefanovic Kojic, J. R.; Markovic, M. D.; Beskoski, V. P.; Vrvic, M. M. High Levan Production by Bacillus Licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. Applied Biochemistry and Biotechnology 2015, 175 (6), 3068–3083. https://doi.org/10.1007/s12010-015-1475-8. in Applied Biochemistry and Biotechnology. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3376 .

Kekez, B. D., Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Stefanović Kojić, Jovana R., Marković, M. D., Beškoski, Vladimir, Vrvić, Miroslav M., "Supplementary data for article: Kekez, B. D.; Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Stefanovic Kojic, J. R.; Markovic, M. D.; Beskoski, V. P.; Vrvic, M. M. High Levan Production by Bacillus Licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. Applied Biochemistry and Biotechnology 2015, 175 (6), 3068–3083. https://doi.org/10.1007/s12010-015-1475-8" in Applied Biochemistry and Biotechnology (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3376 .