TY  - JOUR
AU  - Dojnov, Bijana
AU  - Božić, Nataša
AU  - Bulajic, Nina
AU  - Vujčić, Zoran
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/895
AB  - Immunoblot analysis is not in wide use for diagnosis of invasive candidiasis, mostly because the procedure is not standardized and hence not reliable. This work describes a standardized method for C. albicans antigen extract preparation and immuno-chemical detection. The major improvement of the method is the preparation of combined antigen extract-consisting of the cell wall and cytosol antigens. The fungal cells and lysis buffer were mixed at a 1:3 ratio and disintegrated by ultrasound for six cycles of one minute each. After centrifugation, cytosol antigens were obtained in the supernatant and cell wall antigens were in the precipitate. Precipitate was dissolved in lysis buffer with 2% sodium doclecyl sulfate (SDS) and boiled at 100 degrees C for 2min. After centrifugation, the supernatant was combined with the previous one, so the extract of the combined antigens was obtained in the mixture. With those combined antigen extracts and with sera of three different groups of patients, immunoblot analysis showed sensitivity of 90.2%, specificity of 84.4%, and accuracy of 88.0%.
PB  - Wiley-Liss, Hoboken
T2  - Journal of Clinical Laboratory Analysis
T1  - Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis
VL  - 21
IS  - 6
SP  - 406
EP  - 412
DO  - 10.1002/jcla.20206
ER  - 

@article{
author = "Dojnov, Bijana and Božić, Nataša and Bulajic, Nina and Vujčić, Zoran",
year = "2007",
abstract = "Immunoblot analysis is not in wide use for diagnosis of invasive candidiasis, mostly because the procedure is not standardized and hence not reliable. This work describes a standardized method for C. albicans antigen extract preparation and immuno-chemical detection. The major improvement of the method is the preparation of combined antigen extract-consisting of the cell wall and cytosol antigens. The fungal cells and lysis buffer were mixed at a 1:3 ratio and disintegrated by ultrasound for six cycles of one minute each. After centrifugation, cytosol antigens were obtained in the supernatant and cell wall antigens were in the precipitate. Precipitate was dissolved in lysis buffer with 2% sodium doclecyl sulfate (SDS) and boiled at 100 degrees C for 2min. After centrifugation, the supernatant was combined with the previous one, so the extract of the combined antigens was obtained in the mixture. With those combined antigen extracts and with sera of three different groups of patients, immunoblot analysis showed sensitivity of 90.2%, specificity of 84.4%, and accuracy of 88.0%.",
publisher = "Wiley-Liss, Hoboken",
journal = "Journal of Clinical Laboratory Analysis",
title = "Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis",
volume = "21",
number = "6",
pages = "406-412",
doi = "10.1002/jcla.20206"
}

Dojnov, B., Božić, N., Bulajic, N.,& Vujčić, Z.. (2007). Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis. in Journal of Clinical Laboratory Analysis
Wiley-Liss, Hoboken., 21(6), 406-412.
https://doi.org/10.1002/jcla.20206

Dojnov B, Božić N, Bulajic N, Vujčić Z. Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis. in Journal of Clinical Laboratory Analysis. 2007;21(6):406-412.
doi:10.1002/jcla.20206 .

Dojnov, Bijana, Božić, Nataša, Bulajic, Nina, Vujčić, Zoran, "Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis" in Journal of Clinical Laboratory Analysis, 21, no. 6 (2007):406-412,
https://doi.org/10.1002/jcla.20206 . .