TY  - JOUR
AU  - Apostolović, Danijela
AU  - Grundström, Jeanette
AU  - Peruško, Marija
AU  - Kiewiet, M. B. Gea
AU  - Hamsten, Carl
AU  - Starkhammar, Maria
AU  - van Hage, Marianne
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4839
PB  - Wiley
T2  - Clinical and Translational Allergy
T2  - Clinical and Translational AllergyClin Transl Allergy
T1  - Course of IgE to α-Gal in a Swedish population of α-Gal syndrome patients
VL  - 11
IS  - 10
SP  - e12087
DO  - 10.1002/clt2.12087
ER  - 

@article{
author = "Apostolović, Danijela and Grundström, Jeanette and Peruško, Marija and Kiewiet, M. B. Gea and Hamsten, Carl and Starkhammar, Maria and van Hage, Marianne",
year = "2021",
publisher = "Wiley",
journal = "Clinical and Translational Allergy, Clinical and Translational AllergyClin Transl Allergy",
title = "Course of IgE to α-Gal in a Swedish population of α-Gal syndrome patients",
volume = "11",
number = "10",
pages = "e12087",
doi = "10.1002/clt2.12087"
}

Apostolović, D., Grundström, J., Peruško, M., Kiewiet, M. B. G., Hamsten, C., Starkhammar, M.,& van Hage, M.. (2021). Course of IgE to α-Gal in a Swedish population of α-Gal syndrome patients. in Clinical and Translational Allergy
Wiley., 11(10), e12087.
https://doi.org/10.1002/clt2.12087

Apostolović D, Grundström J, Peruško M, Kiewiet MBG, Hamsten C, Starkhammar M, van Hage M. Course of IgE to α-Gal in a Swedish population of α-Gal syndrome patients. in Clinical and Translational Allergy. 2021;11(10):e12087.
doi:10.1002/clt2.12087 .

Apostolović, Danijela, Grundström, Jeanette, Peruško, Marija, Kiewiet, M. B. Gea, Hamsten, Carl, Starkhammar, Maria, van Hage, Marianne, "Course of IgE to α-Gal in a Swedish population of α-Gal syndrome patients" in Clinical and Translational Allergy, 11, no. 10 (2021):e12087,
https://doi.org/10.1002/clt2.12087 . .

TY  - JOUR
AU  - Krstić-Ristivojević, Maja
AU  - Grundström, Jeanette
AU  - Apostolović, Danijela
AU  - Radomirović, Mirjana Ž.
AU  - Jovanović, Vesna B.
AU  - Radoi, Vlad
AU  - Kiewiet, M. B. Gea
AU  - Vukojević, Vladana
AU  - Ćirković-Veličković, Tanja
AU  - van Hage, Marianne
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4866
AB  - Transepithelial transport of proteins is an important step in the immune response to food allergens. Mammalian meat allergy is characterized by an IgE response against the carbohydrate moiety galactosyl-α-1,3-galactose (α-Gal) present on mammalian glycoproteins and glycolipids, which causes severe allergic reactions several hours after red meat consumption. The delayed reaction may be related to the processing of α-Gal carrying proteins in the gastrointestinal tract. The aim of this study was to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer. We found that α-Gal glycosylation altered protein susceptibility to gastric digestion, where large protein fragments bearing the α-Gal epitope remained for up to 2 h of digestion. Furthermore, α-Gal glycosylation of the protein hampered transcytosis of the protein through the Caco-2 monolayer. α-Gal epitope on the intact protein could be detected in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates. Furthermore, the level of galectin-3 in Caco-2 cells was not affected by the presence of α-Gal glycosylated BSA (bovine serum albumin) (BSA-α-Gal). Taken together, our data add new knowledge and shed light on the digestion and transport of α-Gal glycosylated proteins.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer
VL  - 21
IS  - 16
SP  - 5742
DO  - 10.3390/ijms21165742
ER  - 

@article{
author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Apostolović, Danijela and Radomirović, Mirjana Ž. and Jovanović, Vesna B. and Radoi, Vlad and Kiewiet, M. B. Gea and Vukojević, Vladana and Ćirković-Veličković, Tanja and van Hage, Marianne",
year = "2020",
abstract = "Transepithelial transport of proteins is an important step in the immune response to food allergens. Mammalian meat allergy is characterized by an IgE response against the carbohydrate moiety galactosyl-α-1,3-galactose (α-Gal) present on mammalian glycoproteins and glycolipids, which causes severe allergic reactions several hours after red meat consumption. The delayed reaction may be related to the processing of α-Gal carrying proteins in the gastrointestinal tract. The aim of this study was to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer. We found that α-Gal glycosylation altered protein susceptibility to gastric digestion, where large protein fragments bearing the α-Gal epitope remained for up to 2 h of digestion. Furthermore, α-Gal glycosylation of the protein hampered transcytosis of the protein through the Caco-2 monolayer. α-Gal epitope on the intact protein could be detected in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates. Furthermore, the level of galectin-3 in Caco-2 cells was not affected by the presence of α-Gal glycosylated BSA (bovine serum albumin) (BSA-α-Gal). Taken together, our data add new knowledge and shed light on the digestion and transport of α-Gal glycosylated proteins.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer",
volume = "21",
number = "16",
pages = "5742",
doi = "10.3390/ijms21165742"
}

Krstić-Ristivojević, M., Grundström, J., Apostolović, D., Radomirović, M. Ž., Jovanović, V. B., Radoi, V., Kiewiet, M. B. G., Vukojević, V., Ćirković-Veličković, T.,& van Hage, M.. (2020). Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer. in International Journal of Molecular Sciences
MDPI., 21(16), 5742.
https://doi.org/10.3390/ijms21165742

Krstić-Ristivojević M, Grundström J, Apostolović D, Radomirović MŽ, Jovanović VB, Radoi V, Kiewiet MBG, Vukojević V, Ćirković-Veličković T, van Hage M. Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer. in International Journal of Molecular Sciences. 2020;21(16):5742.
doi:10.3390/ijms21165742 .

Krstić-Ristivojević, Maja, Grundström, Jeanette, Apostolović, Danijela, Radomirović, Mirjana Ž., Jovanović, Vesna B., Radoi, Vlad, Kiewiet, M. B. Gea, Vukojević, Vladana, Ćirković-Veličković, Tanja, van Hage, Marianne, "Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer" in International Journal of Molecular Sciences, 21, no. 16 (2020):5742,
https://doi.org/10.3390/ijms21165742 . .