TY  - JOUR
AU  - Veličković, Luka
AU  - Simović, Ana
AU  - Gligorijević, Nikola
AU  - Thureau, Aurélien
AU  - Obradović, Milica
AU  - Vasović, Tamara
AU  - Sotiroudis, Georgios
AU  - Zoumpanioti, Maria
AU  - Brûlet, Annie
AU  - Ćirković-Veličković, Tanja
AU  - Combet, Sophie
AU  - Nikolić, Milan
AU  - Minić, Simeon
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6273
AB  - This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from the red algae Porphyra spp. (Nori). We purified R-PC from dried Nori flakes with a high purity ratio (A618/A280 ≥ 3.4) in native form (α-helix content 53%). SAXS measurements revealed that R-PC is trimeric ((αβ)3) in solution. The thermal denaturation of α-helix revealed one transition (Tm at 52 °C), while the pH stability study showed R-PC is stable in the pH range 4–8. The thermal treatment of R-PC at 60 °C has detrimental and irreversible effects on R-PC colour and antioxidant capacity (22 % of residual capacity). However, immobilisation of R-PC within calcium alginate beads completely preserves R-PC colour and mainly retains its antioxidant ability (78 % of residual capacity). Results give new insights into the stability of R-PC and preservation of its purple colour and bioactivity by encapsulation in calcium alginate beads.
PB  - Elsevier
T2  - Food Chemistry
T1  - Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant
VL  - 426
SP  - 136669
DO  - 10.1016/j.foodchem.2023.136669
ER  - 

@article{
author = "Veličković, Luka and Simović, Ana and Gligorijević, Nikola and Thureau, Aurélien and Obradović, Milica and Vasović, Tamara and Sotiroudis, Georgios and Zoumpanioti, Maria and Brûlet, Annie and Ćirković-Veličković, Tanja and Combet, Sophie and Nikolić, Milan and Minić, Simeon",
year = "2023",
abstract = "This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from the red algae Porphyra spp. (Nori). We purified R-PC from dried Nori flakes with a high purity ratio (A618/A280 ≥ 3.4) in native form (α-helix content 53%). SAXS measurements revealed that R-PC is trimeric ((αβ)3) in solution. The thermal denaturation of α-helix revealed one transition (Tm at 52 °C), while the pH stability study showed R-PC is stable in the pH range 4–8. The thermal treatment of R-PC at 60 °C has detrimental and irreversible effects on R-PC colour and antioxidant capacity (22 % of residual capacity). However, immobilisation of R-PC within calcium alginate beads completely preserves R-PC colour and mainly retains its antioxidant ability (78 % of residual capacity). Results give new insights into the stability of R-PC and preservation of its purple colour and bioactivity by encapsulation in calcium alginate beads.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant",
volume = "426",
pages = "136669",
doi = "10.1016/j.foodchem.2023.136669"
}

Veličković, L., Simović, A., Gligorijević, N., Thureau, A., Obradović, M., Vasović, T., Sotiroudis, G., Zoumpanioti, M., Brûlet, A., Ćirković-Veličković, T., Combet, S., Nikolić, M.,& Minić, S.. (2023). Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant. in Food Chemistry
Elsevier., 426, 136669.
https://doi.org/10.1016/j.foodchem.2023.136669

Veličković L, Simović A, Gligorijević N, Thureau A, Obradović M, Vasović T, Sotiroudis G, Zoumpanioti M, Brûlet A, Ćirković-Veličković T, Combet S, Nikolić M, Minić S. Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant. in Food Chemistry. 2023;426:136669.
doi:10.1016/j.foodchem.2023.136669 .

Veličković, Luka, Simović, Ana, Gligorijević, Nikola, Thureau, Aurélien, Obradović, Milica, Vasović, Tamara, Sotiroudis, Georgios, Zoumpanioti, Maria, Brûlet, Annie, Ćirković-Veličković, Tanja, Combet, Sophie, Nikolić, Milan, Minić, Simeon, "Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant" in Food Chemistry, 426 (2023):136669,
https://doi.org/10.1016/j.foodchem.2023.136669 . .

TY  - JOUR
AU  - Veličković, Luka
AU  - Simović, Ana
AU  - Gligorijević, Nikola
AU  - Thureau, Aurelien
AU  - Obradović, Milica
AU  - Vasović, Tamara
AU  - Sotiroudis, Georgios
AU  - Zoumpanioti, Maria
AU  - Brûlet, Annie
AU  - Ćirković-Veličković, Tanja
AU  - Combet, Sophie
AU  - Nikolić, Milan
AU  - Minić, Simeon
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6420
AB  - This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from thered algae Porphyra spp. (Nori). We purified R-PC from dried Nori flakes with a high purity ratio (A618/A280 ≥ 3.4)in native form (α-helix content 53%). SAXS measurements revealed that R-PC is trimeric ((αβ)3) in solution. Thethermal denaturation of α-helix revealed one transition (Tm at 52 ◦C), while the pH stability study showed R-PC isstable in the pH range 4–8. The thermal treatment of R-PC at 60 ◦C has detrimental and irreversible effects on RPCcolour and antioxidant capacity (22 % of residual capacity). However, immobilisation of R-PC within calciumalginate beads completely preserves R-PC colour and mainly retains its antioxidant ability (78 % of residualcapacity). Results give new insights into the stability of R-PC and preservation of its purple colour and bioactivityby encapsulation in calcium alginate beads.
PB  - Elsevier
T2  - Food Chemistry
T1  - Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant
VL  - 426
SP  - 136669
DO  - 10.1016/j.foodchem.2023.136669
ER  - 

@article{
author = "Veličković, Luka and Simović, Ana and Gligorijević, Nikola and Thureau, Aurelien and Obradović, Milica and Vasović, Tamara and Sotiroudis, Georgios and Zoumpanioti, Maria and Brûlet, Annie and Ćirković-Veličković, Tanja and Combet, Sophie and Nikolić, Milan and Minić, Simeon",
year = "2023",
abstract = "This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from thered algae Porphyra spp. (Nori). We purified R-PC from dried Nori flakes with a high purity ratio (A618/A280 ≥ 3.4)in native form (α-helix content 53%). SAXS measurements revealed that R-PC is trimeric ((αβ)3) in solution. Thethermal denaturation of α-helix revealed one transition (Tm at 52 ◦C), while the pH stability study showed R-PC isstable in the pH range 4–8. The thermal treatment of R-PC at 60 ◦C has detrimental and irreversible effects on RPCcolour and antioxidant capacity (22 % of residual capacity). However, immobilisation of R-PC within calciumalginate beads completely preserves R-PC colour and mainly retains its antioxidant ability (78 % of residualcapacity). Results give new insights into the stability of R-PC and preservation of its purple colour and bioactivityby encapsulation in calcium alginate beads.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant",
volume = "426",
pages = "136669",
doi = "10.1016/j.foodchem.2023.136669"
}

Veličković, L., Simović, A., Gligorijević, N., Thureau, A., Obradović, M., Vasović, T., Sotiroudis, G., Zoumpanioti, M., Brûlet, A., Ćirković-Veličković, T., Combet, S., Nikolić, M.,& Minić, S.. (2023). Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant. in Food Chemistry
Elsevier., 426, 136669.
https://doi.org/10.1016/j.foodchem.2023.136669

Veličković L, Simović A, Gligorijević N, Thureau A, Obradović M, Vasović T, Sotiroudis G, Zoumpanioti M, Brûlet A, Ćirković-Veličković T, Combet S, Nikolić M, Minić S. Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant. in Food Chemistry. 2023;426:136669.
doi:10.1016/j.foodchem.2023.136669 .

Veličković, Luka, Simović, Ana, Gligorijević, Nikola, Thureau, Aurelien, Obradović, Milica, Vasović, Tamara, Sotiroudis, Georgios, Zoumpanioti, Maria, Brûlet, Annie, Ćirković-Veličković, Tanja, Combet, Sophie, Nikolić, Milan, Minić, Simeon, "Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant" in Food Chemistry, 426 (2023):136669,
https://doi.org/10.1016/j.foodchem.2023.136669 . .

TY  - CONF
AU  - Veličković, Luka
AU  - Simović, Ana
AU  - Gligorijević, Nikola
AU  - Obradović, Milica
AU  - Sotiroudis, Georgios
AU  - Zoumpanioti, Maria
AU  - Minić, Simeon
AU  - Nikolić, Milan
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6315
AB  - Purple R-phycocyanin is a protein from red algae with the potential for application in the food industry (colorant) and wastewater treatment (binding of heavy metals). Analytical grade R-phycocyanin was purified from the buffered extract of dried Nori flakes (Porphyra spp.) by combining ammonium sulfate precipitation, hydroxyapatite, and DEAE-Sepharose column chromatography. The multimeric protein had absorption maxima characteristic for phycoerythrobilin (at 580 nm) and phycocyanobilin (at 640 nm) chromophores, high α- helical content, and melting temperature of 52°C. The secondary R-PC structure was stable under a wide range of pH values (3–9). R-phycocyanin immobilized in calcium alginate beads showed increased thermal stability and preserved antioxidant activity.
AB  - Ljubičasti R-fikocijanin je protein crvenih algi sa mogućnostima primene u industriji hrane (kolorant) i za tretman otpadnih voda (vezuje teške metale). R-fikocijanin analitičke čistoće je izolovan iz puferisanog ekstrakta osušenih Nori algi (Porphyra spp.), kombinacijom taloženja amonijum-sulfatom, hidroksiapatitne i hromatografije na DEAE-Sepharose koloni. Multimerni protein imao je apsorpcione maksimume karakteristične za fikoeritrobilinsku (na 580 nm) i fikocijanobilinsku (na 640 nm) hromoforu, visok sadržaj α-zavojnica i temperaturu topljenja od 52°C. Sekundarna struktura proteina bila je stabilna u širokom rasponu pH vrednosti (3–9). R-fikocijanin imobilisan u kuglice kalcijum-alginata pokazao je povećanu toplotnu stabilnost i očuvana antioksidativna svojstva.
PB  - Belgrade : Serbian Chemical Society
C3  - 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia
T1  - Purification and structural characterization of R-phycocyanin
T1  - Prečišćavanje i strukturna karakterizacija R-fikocijanina
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6315
ER  - 

@conference{
author = "Veličković, Luka and Simović, Ana and Gligorijević, Nikola and Obradović, Milica and Sotiroudis, Georgios and Zoumpanioti, Maria and Minić, Simeon and Nikolić, Milan",
year = "2023",
abstract = "Purple R-phycocyanin is a protein from red algae with the potential for application in the food industry (colorant) and wastewater treatment (binding of heavy metals). Analytical grade R-phycocyanin was purified from the buffered extract of dried Nori flakes (Porphyra spp.) by combining ammonium sulfate precipitation, hydroxyapatite, and DEAE-Sepharose column chromatography. The multimeric protein had absorption maxima characteristic for phycoerythrobilin (at 580 nm) and phycocyanobilin (at 640 nm) chromophores, high α- helical content, and melting temperature of 52°C. The secondary R-PC structure was stable under a wide range of pH values (3–9). R-phycocyanin immobilized in calcium alginate beads showed increased thermal stability and preserved antioxidant activity., Ljubičasti R-fikocijanin je protein crvenih algi sa mogućnostima primene u industriji hrane (kolorant) i za tretman otpadnih voda (vezuje teške metale). R-fikocijanin analitičke čistoće je izolovan iz puferisanog ekstrakta osušenih Nori algi (Porphyra spp.), kombinacijom taloženja amonijum-sulfatom, hidroksiapatitne i hromatografije na DEAE-Sepharose koloni. Multimerni protein imao je apsorpcione maksimume karakteristične za fikoeritrobilinsku (na 580 nm) i fikocijanobilinsku (na 640 nm) hromoforu, visok sadržaj α-zavojnica i temperaturu topljenja od 52°C. Sekundarna struktura proteina bila je stabilna u širokom rasponu pH vrednosti (3–9). R-fikocijanin imobilisan u kuglice kalcijum-alginata pokazao je povećanu toplotnu stabilnost i očuvana antioksidativna svojstva.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia",
title = "Purification and structural characterization of R-phycocyanin, Prečišćavanje i strukturna karakterizacija R-fikocijanina",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6315"
}

Veličković, L., Simović, A., Gligorijević, N., Obradović, M., Sotiroudis, G., Zoumpanioti, M., Minić, S.,& Nikolić, M.. (2023). Purification and structural characterization of R-phycocyanin. in 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia
Belgrade : Serbian Chemical Society., 53-53.
https://hdl.handle.net/21.15107/rcub_cherry_6315

Veličković L, Simović A, Gligorijević N, Obradović M, Sotiroudis G, Zoumpanioti M, Minić S, Nikolić M. Purification and structural characterization of R-phycocyanin. in 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia. 2023;:53-53.
https://hdl.handle.net/21.15107/rcub_cherry_6315 .

Veličković, Luka, Simović, Ana, Gligorijević, Nikola, Obradović, Milica, Sotiroudis, Georgios, Zoumpanioti, Maria, Minić, Simeon, Nikolić, Milan, "Purification and structural characterization of R-phycocyanin" in 59th Meeting of the Serbian Chemical Society, Book of Abstracts, June 1-2, 2023, Novi Sad, Serbia (2023):53-53,
https://hdl.handle.net/21.15107/rcub_cherry_6315 .

TY  - CONF
AU  - Obradović, Milica
AU  - Nikolić, Milan
AU  - Minić, Simeon L.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5787
AB  - Phycobiliproteins are major photosynthetic accessory pigments in cyanobacteria and red
algae. Their vivid colours arise from covalently attached tetrapyrrole chromophores. The
exciting characteristic of tetrapyrrole chromophores is the ability to bind metal ions. Heavy
metals are among the most abundant and most dangerous environmental pollutants, and
their removal from the environment is a crucial challenge. Therefore, utilizing PBPs-metal
binding properties could be helpful in heavy metal detection and/or removal. The main aim
of this study is to characterize the binding of selected heavy metal ions (Hg2+, Pb2+, Cd2+)
to R-phycocyanin (R-PC) isolated and purified from red algae Porphyra spp.The protein
fluorescence quenching approach revealed the strong binding affinity of R-PC to Hg2+
(Kd~0.1 μM), while protein binding to Pb2+ and Cd2+ is lower (Kd~3 μM) but still in the
high to moderate range. Circular dichroism spectroscopy demonstrated the ability of Hg2+,
Pb2+ and Cd2+ to slightly -helical content) in
R-PC. Our results indicatethat R-PC could beexploited as a potential biosensor for heavy
metal ions detection (especially Hg2+) in aquatic systems as well as in their removal from
the environment (e.g. waste-water management).
PB  - Belgrade : Faculty of Chemistry
C3  - Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
T1  - R-Phycocyanin from red algae Porphyra spp: Binding of selected heavy metal ions
SP  - 113
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5787
ER  - 

@conference{
author = "Obradović, Milica and Nikolić, Milan and Minić, Simeon L.",
year = "2022",
abstract = "Phycobiliproteins are major photosynthetic accessory pigments in cyanobacteria and red
algae. Their vivid colours arise from covalently attached tetrapyrrole chromophores. The
exciting characteristic of tetrapyrrole chromophores is the ability to bind metal ions. Heavy
metals are among the most abundant and most dangerous environmental pollutants, and
their removal from the environment is a crucial challenge. Therefore, utilizing PBPs-metal
binding properties could be helpful in heavy metal detection and/or removal. The main aim
of this study is to characterize the binding of selected heavy metal ions (Hg2+, Pb2+, Cd2+)
to R-phycocyanin (R-PC) isolated and purified from red algae Porphyra spp.The protein
fluorescence quenching approach revealed the strong binding affinity of R-PC to Hg2+
(Kd~0.1 μM), while protein binding to Pb2+ and Cd2+ is lower (Kd~3 μM) but still in the
high to moderate range. Circular dichroism spectroscopy demonstrated the ability of Hg2+,
Pb2+ and Cd2+ to slightly -helical content) in
R-PC. Our results indicatethat R-PC could beexploited as a potential biosensor for heavy
metal ions detection (especially Hg2+) in aquatic systems as well as in their removal from
the environment (e.g. waste-water management).",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia",
title = "R-Phycocyanin from red algae Porphyra spp: Binding of selected heavy metal ions",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5787"
}

Obradović, M., Nikolić, M.,& Minić, S. L.. (2022). R-Phycocyanin from red algae Porphyra spp: Binding of selected heavy metal ions. in Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
Belgrade : Faculty of Chemistry., 113-113.
https://hdl.handle.net/21.15107/rcub_cherry_5787

Obradović M, Nikolić M, Minić SL. R-Phycocyanin from red algae Porphyra spp: Binding of selected heavy metal ions. in Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. 2022;:113-113.
https://hdl.handle.net/21.15107/rcub_cherry_5787 .

Obradović, Milica, Nikolić, Milan, Minić, Simeon L., "R-Phycocyanin from red algae Porphyra spp: Binding of selected heavy metal ions" in Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia (2022):113-113,
https://hdl.handle.net/21.15107/rcub_cherry_5787 .

TY  - THES
AU  - Obradović, Milica
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5597
AB  - R-fikocijanin (R-PC) je pomoćni fotosintetski pigment crvenih algi. Ubraja se u grupu fikobilinskih proteina, u koju spadaju i R-fikoeritrin i alofikocijanin, koji zajedno sa R-fikocijaninom grade ćelijske strukture koje za zadatak imaju apsorpciju i usmeravanje svetlosti u procesu fotosinteze. Značajna strukturna karakteristika fikobilinskih proteina je prisustvo kovalentno vezanih tetrapirolnih hromofora. Interesantno, navedene hromofore imaju sposobnost vezivanja jona metala. Teški metali su među najzastupljenijim i najopasnijim zagađivačima životne sredine, a njihovo uklanjanje iz životne sredine predstavlja važan naučni izazov. Shodno tome, sposobnost fikobilinskih proteina da vezuju jone metala može biti iskorišćena za detekciju i uklanjanje jona teških metala. Glavni cilj ovog rada bio je karakterizacija vezivanje odabranih jona teških metala (Hg2+, Pb2+, Cd2+) za R-fikocijanin (R-PC) izolovan i prečišćen iz crvene alge Porphyra spp. R-fikocijanin je izolovan i prečišćen uz pomoć standardizovanih procedura: hromatografije na hidroksiapatitu i jonoizmenjivačke hromatografije (DEAE-Sepharose). Potom je okarakterisano vezivanje navedenih teških metala za R-fikocijanin, kao i njihov uticaj na stabilnost ovog proteina, pomoću UV-VIS spektrofotometrije, spektrofluorimetrije i cirkularnog dihroizma. Dobijeni rezultati ukazuju da se navedeni joni metala vezuju za R-PC i menjaju konformaciju njegovih hromofora, kao i sadržaj sekundarnih struktura, pri čemu su najveći afinitet vezivanja pokazali joni žive. Ova studija ukazuje na značajan potencijal R-PC u industrijske svrhe, najpre kao biosenzora za detekciju jona teških metala, a zatim i za njihovo specifično uklanjanje iz životne sredine. Shodno tome, kompleksiranje jona metala od strane R-PC može doprineti očuvanju ekosistema od štetnog uticaja teških metala.
T1  - Vezivanje odabranih jona teških metala za R-fikocijanin iz crvene alge Porphyra spp.
SP  - 1
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5597
ER  - 

@mastersthesis{
author = "Obradović, Milica",
year = "2022",
abstract = "R-fikocijanin (R-PC) je pomoćni fotosintetski pigment crvenih algi. Ubraja se u grupu fikobilinskih proteina, u koju spadaju i R-fikoeritrin i alofikocijanin, koji zajedno sa R-fikocijaninom grade ćelijske strukture koje za zadatak imaju apsorpciju i usmeravanje svetlosti u procesu fotosinteze. Značajna strukturna karakteristika fikobilinskih proteina je prisustvo kovalentno vezanih tetrapirolnih hromofora. Interesantno, navedene hromofore imaju sposobnost vezivanja jona metala. Teški metali su među najzastupljenijim i najopasnijim zagađivačima životne sredine, a njihovo uklanjanje iz životne sredine predstavlja važan naučni izazov. Shodno tome, sposobnost fikobilinskih proteina da vezuju jone metala može biti iskorišćena za detekciju i uklanjanje jona teških metala. Glavni cilj ovog rada bio je karakterizacija vezivanje odabranih jona teških metala (Hg2+, Pb2+, Cd2+) za R-fikocijanin (R-PC) izolovan i prečišćen iz crvene alge Porphyra spp. R-fikocijanin je izolovan i prečišćen uz pomoć standardizovanih procedura: hromatografije na hidroksiapatitu i jonoizmenjivačke hromatografije (DEAE-Sepharose). Potom je okarakterisano vezivanje navedenih teških metala za R-fikocijanin, kao i njihov uticaj na stabilnost ovog proteina, pomoću UV-VIS spektrofotometrije, spektrofluorimetrije i cirkularnog dihroizma. Dobijeni rezultati ukazuju da se navedeni joni metala vezuju za R-PC i menjaju konformaciju njegovih hromofora, kao i sadržaj sekundarnih struktura, pri čemu su najveći afinitet vezivanja pokazali joni žive. Ova studija ukazuje na značajan potencijal R-PC u industrijske svrhe, najpre kao biosenzora za detekciju jona teških metala, a zatim i za njihovo specifično uklanjanje iz životne sredine. Shodno tome, kompleksiranje jona metala od strane R-PC može doprineti očuvanju ekosistema od štetnog uticaja teških metala.",
title = "Vezivanje odabranih jona teških metala za R-fikocijanin iz crvene alge Porphyra spp.",
pages = "1-30",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5597"
}

Obradović, M.. (2022). Vezivanje odabranih jona teških metala za R-fikocijanin iz crvene alge Porphyra spp.. , 1-30.
https://hdl.handle.net/21.15107/rcub_cherry_5597

Obradović M. Vezivanje odabranih jona teških metala za R-fikocijanin iz crvene alge Porphyra spp.. 2022;:1-30.
https://hdl.handle.net/21.15107/rcub_cherry_5597 .

Obradović, Milica, "Vezivanje odabranih jona teških metala za R-fikocijanin iz crvene alge Porphyra spp." (2022):1-30,
https://hdl.handle.net/21.15107/rcub_cherry_5597 .

TY  - THES
AU  - Obradović, Milica
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4712
AB  - R-fikocijanin je obojeni pomoćni fotosintetski pigment crvenih algi. Ubraja se u grupu fikobilinskih proteina, u koju spadaju i R-fikoeritrin i alofikocijanin, koji zajedno sa R-fikocijaninom grade ćelijske strukture koje za zadatak imaju apsorociju i usmeravanje svetlosti u procesu fotosinteze. Kroz ovaj rad će R-fikocijanin biti izolovan i prečišćen uz pomoć standardizovanih procedura, hromatografije na hidroksiapatitu i jonoizmenjivačke hromatografije na DEAE-Sepharose. Zatim će biti okarakterisano vezivanje odabranih mikroelemenata (Cu2+, Zn2+, Fe2+, Fe3+) za R-fikocijanin pomoću UV-VIS spektrofotometrije i spektrofluorimetrije
Ova studija je izvođena u cilju ispitivanja potencijalnih benefita primene nori algi, roda Porphyra, u ishrani ljudi, a ovi benefiti su najviše povezani sa značajnim antioksidativnim aktivnostima fikobiliproteina iz crvenih algi. Dobijeni rezultati ukazuju da se navedeni joni metala mogu vezivati za R-fikocijanin i menjati konformaciju njegovih hromofora, pri čemu su najveći afinitet vezivanja pokazali joni bakra. Shodno tome, kompleksiranje jona metala od strane R-fikocijanina može značajno poboljšati njihovu biodostupnst, ali i sprečiti metal-indukovano generisanje reaktivnih kiseoničnih vrsta.
T1  - Metal - vezujuća svojstva R-fikocijanina iz crvene alge Porphyra spp.
SP  - 2
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4712
ER  - 

@misc{
author = "Obradović, Milica",
year = "2021",
abstract = "R-fikocijanin je obojeni pomoćni fotosintetski pigment crvenih algi. Ubraja se u grupu fikobilinskih proteina, u koju spadaju i R-fikoeritrin i alofikocijanin, koji zajedno sa R-fikocijaninom grade ćelijske strukture koje za zadatak imaju apsorociju i usmeravanje svetlosti u procesu fotosinteze. Kroz ovaj rad će R-fikocijanin biti izolovan i prečišćen uz pomoć standardizovanih procedura, hromatografije na hidroksiapatitu i jonoizmenjivačke hromatografije na DEAE-Sepharose. Zatim će biti okarakterisano vezivanje odabranih mikroelemenata (Cu2+, Zn2+, Fe2+, Fe3+) za R-fikocijanin pomoću UV-VIS spektrofotometrije i spektrofluorimetrije
Ova studija je izvođena u cilju ispitivanja potencijalnih benefita primene nori algi, roda Porphyra, u ishrani ljudi, a ovi benefiti su najviše povezani sa značajnim antioksidativnim aktivnostima fikobiliproteina iz crvenih algi. Dobijeni rezultati ukazuju da se navedeni joni metala mogu vezivati za R-fikocijanin i menjati konformaciju njegovih hromofora, pri čemu su najveći afinitet vezivanja pokazali joni bakra. Shodno tome, kompleksiranje jona metala od strane R-fikocijanina može značajno poboljšati njihovu biodostupnst, ali i sprečiti metal-indukovano generisanje reaktivnih kiseoničnih vrsta.",
title = "Metal - vezujuća svojstva R-fikocijanina iz crvene alge Porphyra spp.",
pages = "2-30",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4712"
}

Obradović, M.. (2021). Metal - vezujuća svojstva R-fikocijanina iz crvene alge Porphyra spp.. , 2-30.
https://hdl.handle.net/21.15107/rcub_cherry_4712

Obradović M. Metal - vezujuća svojstva R-fikocijanina iz crvene alge Porphyra spp.. 2021;:2-30.
https://hdl.handle.net/21.15107/rcub_cherry_4712 .

Obradović, Milica, "Metal - vezujuća svojstva R-fikocijanina iz crvene alge Porphyra spp." (2021):2-30,
https://hdl.handle.net/21.15107/rcub_cherry_4712 .