TY  - CONF
AU  - Krishna de Guzman, M.
AU  - Jovanović, V.
AU  - Jung, J.
AU  - Kim, J.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4909
AB  - The high bioavailability of microplastics (MP) to marine biota has led to the contamination of seafood products. The safety of shellfish as food has become questionable due to the potential health risks associated with MP. As such, microplastics ingestion by commonly consumed shellfish is being investigated. In this study, market samples of clams (Ruditapes philippinarum, n=101) from South Korea were segregated into two different sizes (small and large) and analysed for microplastics contamination. Using alkaline digestion, MP were extracted and subsequently quantified using two of the most commonly used techniques in the field- Nile red staining (NRS) and μFTIR imaging [1], [2]. With NRS, the small (n= 51) and large (n= 50) clams were analysed individually. On the other hand, 8 composite subsamples (30 small and 31 large clams) were subjected to μFTIR imaging. The average MP concentration based on NRS was 4.3 ± 5.2 MP/ g ww (wet weight of soft tissue) considering both small and large clams. In the subsamples, 3.2 ± 1.6 MP/ g ww and 3.8 ± 1.7 MP/g ww were detected based on μFTIR and NRS, respectively. NRS showed 18-75% higher MP quantity compared to μFTIR. This was considered to be a consequence of the co-staining of remnants of undigested biological which was confirmed by additional staining using DAPI. Due to this overestimation, only μFTIR data was utilized for the comparative analysis of the differently sized clams. The MP abundance in both groups was comparable, with the small and large clams having 2.7 ± 1.7 MP/g ww and 3.6 ± 1.6 MP/g ww, respectively. Similarly, there was no significant difference observed in the concentration of MP fibers and MP non-fibers. In the small clams, only PS (polystyrene) and PET (polyethylene terephthalate) were identified from the fibers. In contrast, PP (polypropylene), PS (polystyrene), PE, and PET were found from the fibers that came from the large clams. The same 4 synthetic polymers were detected from the non-fibers obtained from both groups. Overall, PS was the most prominent type, accounting for 35- 49% of total detected MP. In terms of MP size, 20-50μm was the most abundant followed by 50-100μm. Relative to the weight of the samples, the quantity of MP, classified according to size and polymer type, was similar in both groups.  In summary, the size of the clams had no effect on the MP content as suggested by the analogous concentrations of MP with respect to shape, size, and polymer identity. In addition, despite the simplicity, low-cost, and growing popularity of NRS, this method should be used with caution due to its propensity to overestimate MP quantity.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - Comparative Profiling of Microplastics in Differently sized Manila Clams from South Korea by Nile Red Staining and μFTIR
SP  - 57
EP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4909
ER  - 

@conference{
author = "Krishna de Guzman, M. and Jovanović, V. and Jung, J. and Kim, J. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "The high bioavailability of microplastics (MP) to marine biota has led to the contamination of seafood products. The safety of shellfish as food has become questionable due to the potential health risks associated with MP. As such, microplastics ingestion by commonly consumed shellfish is being investigated. In this study, market samples of clams (Ruditapes philippinarum, n=101) from South Korea were segregated into two different sizes (small and large) and analysed for microplastics contamination. Using alkaline digestion, MP were extracted and subsequently quantified using two of the most commonly used techniques in the field- Nile red staining (NRS) and μFTIR imaging [1], [2]. With NRS, the small (n= 51) and large (n= 50) clams were analysed individually. On the other hand, 8 composite subsamples (30 small and 31 large clams) were subjected to μFTIR imaging. The average MP concentration based on NRS was 4.3 ± 5.2 MP/ g ww (wet weight of soft tissue) considering both small and large clams. In the subsamples, 3.2 ± 1.6 MP/ g ww and 3.8 ± 1.7 MP/g ww were detected based on μFTIR and NRS, respectively. NRS showed 18-75% higher MP quantity compared to μFTIR. This was considered to be a consequence of the co-staining of remnants of undigested biological which was confirmed by additional staining using DAPI. Due to this overestimation, only μFTIR data was utilized for the comparative analysis of the differently sized clams. The MP abundance in both groups was comparable, with the small and large clams having 2.7 ± 1.7 MP/g ww and 3.6 ± 1.6 MP/g ww, respectively. Similarly, there was no significant difference observed in the concentration of MP fibers and MP non-fibers. In the small clams, only PS (polystyrene) and PET (polyethylene terephthalate) were identified from the fibers. In contrast, PP (polypropylene), PS (polystyrene), PE, and PET were found from the fibers that came from the large clams. The same 4 synthetic polymers were detected from the non-fibers obtained from both groups. Overall, PS was the most prominent type, accounting for 35- 49% of total detected MP. In terms of MP size, 20-50μm was the most abundant followed by 50-100μm. Relative to the weight of the samples, the quantity of MP, classified according to size and polymer type, was similar in both groups.  In summary, the size of the clams had no effect on the MP content as suggested by the analogous concentrations of MP with respect to shape, size, and polymer identity. In addition, despite the simplicity, low-cost, and growing popularity of NRS, this method should be used with caution due to its propensity to overestimate MP quantity.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "Comparative Profiling of Microplastics in Differently sized Manila Clams from South Korea by Nile Red Staining and μFTIR",
pages = "57-57",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4909"
}

Krishna de Guzman, M., Jovanović, V., Jung, J., Kim, J.,& Ćirković-Veličković, T.. (2021). Comparative Profiling of Microplastics in Differently sized Manila Clams from South Korea by Nile Red Staining and μFTIR. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 57-57.
https://hdl.handle.net/21.15107/rcub_cherry_4909

Krishna de Guzman M, Jovanović V, Jung J, Kim J, Ćirković-Veličković T. Comparative Profiling of Microplastics in Differently sized Manila Clams from South Korea by Nile Red Staining and μFTIR. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:57-57.
https://hdl.handle.net/21.15107/rcub_cherry_4909 .

Krishna de Guzman, M., Jovanović, V., Jung, J., Kim, J., Ćirković-Veličković, Tanja, "Comparative Profiling of Microplastics in Differently sized Manila Clams from South Korea by Nile Red Staining and μFTIR" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):57-57,
https://hdl.handle.net/21.15107/rcub_cherry_4909 .

TY  - DATA
AU  - Petković, Branka B.
AU  - Milčić, Miloš K.
AU  - Stanković, Dalibor
AU  - Stambolić, I.
AU  - Manojlović, Dragan D.
AU  - Jovanović, V. M.
AU  - Sovilj, Sofija P.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3473
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Electrochimica Acta
T1  - Supplementary data for article: Petković, B. B.; Milčić, M. K.; Stanković, D.; Stambolic, I.; Manojlović, D. D.; Jovanovic, V. M.; Sovilj, S. P. Complexation Ability of Octaazamacrocyclic Ligand toward Co2+, Ni2+, Cu2+ and Zn2+ Metal Cations: Experimental and Theoretical Study. Electrochimica Acta 2013, 89, 680–687. https://doi.org/10.1016/j.electacta.2012.11.100
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3473
ER  - 

@misc{
author = "Petković, Branka B. and Milčić, Miloš K. and Stanković, Dalibor and Stambolić, I. and Manojlović, Dragan D. and Jovanović, V. M. and Sovilj, Sofija P.",
year = "2013",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Electrochimica Acta",
title = "Supplementary data for article: Petković, B. B.; Milčić, M. K.; Stanković, D.; Stambolic, I.; Manojlović, D. D.; Jovanovic, V. M.; Sovilj, S. P. Complexation Ability of Octaazamacrocyclic Ligand toward Co2+, Ni2+, Cu2+ and Zn2+ Metal Cations: Experimental and Theoretical Study. Electrochimica Acta 2013, 89, 680–687. https://doi.org/10.1016/j.electacta.2012.11.100",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3473"
}

Petković, B. B., Milčić, M. K., Stanković, D., Stambolić, I., Manojlović, D. D., Jovanović, V. M.,& Sovilj, S. P.. (2013). Supplementary data for article: Petković, B. B.; Milčić, M. K.; Stanković, D.; Stambolic, I.; Manojlović, D. D.; Jovanovic, V. M.; Sovilj, S. P. Complexation Ability of Octaazamacrocyclic Ligand toward Co2+, Ni2+, Cu2+ and Zn2+ Metal Cations: Experimental and Theoretical Study. Electrochimica Acta 2013, 89, 680–687. https://doi.org/10.1016/j.electacta.2012.11.100. in Electrochimica Acta
Pergamon-Elsevier Science Ltd, Oxford..
https://hdl.handle.net/21.15107/rcub_cherry_3473

Petković BB, Milčić MK, Stanković D, Stambolić I, Manojlović DD, Jovanović VM, Sovilj SP. Supplementary data for article: Petković, B. B.; Milčić, M. K.; Stanković, D.; Stambolic, I.; Manojlović, D. D.; Jovanovic, V. M.; Sovilj, S. P. Complexation Ability of Octaazamacrocyclic Ligand toward Co2+, Ni2+, Cu2+ and Zn2+ Metal Cations: Experimental and Theoretical Study. Electrochimica Acta 2013, 89, 680–687. https://doi.org/10.1016/j.electacta.2012.11.100. in Electrochimica Acta. 2013;.
https://hdl.handle.net/21.15107/rcub_cherry_3473 .

Petković, Branka B., Milčić, Miloš K., Stanković, Dalibor, Stambolić, I., Manojlović, Dragan D., Jovanović, V. M., Sovilj, Sofija P., "Supplementary data for article: Petković, B. B.; Milčić, M. K.; Stanković, D.; Stambolic, I.; Manojlović, D. D.; Jovanovic, V. M.; Sovilj, S. P. Complexation Ability of Octaazamacrocyclic Ligand toward Co2+, Ni2+, Cu2+ and Zn2+ Metal Cations: Experimental and Theoretical Study. Electrochimica Acta 2013, 89, 680–687. https://doi.org/10.1016/j.electacta.2012.11.100" in Electrochimica Acta (2013),
https://hdl.handle.net/21.15107/rcub_cherry_3473 .

TY  - JOUR
AU  - Petković, Branka B.
AU  - Milčić, Miloš K.
AU  - Stanković, Dalibor
AU  - Stambolić, I.
AU  - Manojlović, Dragan D.
AU  - Jovanović, V. M.
AU  - Sovilj, Sofija P.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1607
AB  - The complexation reactions between octaazamacrocyclic ligand N,N',N '',N'"-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc) and Co2+, Ni2+, Zn2+ and Cu2+ metal cations have been studied conductometrically in acetonitrile and aqueous solutions. The conductance data exhibit different stoichiometry of the complexes in two studied solutions. In most cases 1:1 (ML) complexes were formed while in some cases 2:1 (M2L) complexes are evident. The stability constants of the resulting 1:1 complexes were determined by computer fitting of the conductance/mole ratio data. Theoretical DFT calculations were used to explain metal binding capability of tpmc ligand and the binding sequences were found to follow the order: Cu2+  gt  gt  Ni2+  gt  Co2+  gt  Zn2+. The combination of results obtained by this theoretical approach and conductometric study successfully explains sensitivity and selectivity of recently developed tpmc based ISE and could play a guiding role in planning experiments with large macrocyclic ligands.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Electrochimica Acta
T1  - Complexation ability of octaazamacrocyclic ligand toward Co2+, Ni2+, Cu2+ and Zn2+ metal cations: Experimental and theoretical study
VL  - 89
SP  - 680
EP  - 687
DO  - 10.1016/j.electacta.2012.11.100
ER  - 

@article{
author = "Petković, Branka B. and Milčić, Miloš K. and Stanković, Dalibor and Stambolić, I. and Manojlović, Dragan D. and Jovanović, V. M. and Sovilj, Sofija P.",
year = "2013",
abstract = "The complexation reactions between octaazamacrocyclic ligand N,N',N '',N'"-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc) and Co2+, Ni2+, Zn2+ and Cu2+ metal cations have been studied conductometrically in acetonitrile and aqueous solutions. The conductance data exhibit different stoichiometry of the complexes in two studied solutions. In most cases 1:1 (ML) complexes were formed while in some cases 2:1 (M2L) complexes are evident. The stability constants of the resulting 1:1 complexes were determined by computer fitting of the conductance/mole ratio data. Theoretical DFT calculations were used to explain metal binding capability of tpmc ligand and the binding sequences were found to follow the order: Cu2+  gt  gt  Ni2+  gt  Co2+  gt  Zn2+. The combination of results obtained by this theoretical approach and conductometric study successfully explains sensitivity and selectivity of recently developed tpmc based ISE and could play a guiding role in planning experiments with large macrocyclic ligands.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Electrochimica Acta",
title = "Complexation ability of octaazamacrocyclic ligand toward Co2+, Ni2+, Cu2+ and Zn2+ metal cations: Experimental and theoretical study",
volume = "89",
pages = "680-687",
doi = "10.1016/j.electacta.2012.11.100"
}

Petković, B. B., Milčić, M. K., Stanković, D., Stambolić, I., Manojlović, D. D., Jovanović, V. M.,& Sovilj, S. P.. (2013). Complexation ability of octaazamacrocyclic ligand toward Co2+, Ni2+, Cu2+ and Zn2+ metal cations: Experimental and theoretical study. in Electrochimica Acta
Pergamon-Elsevier Science Ltd, Oxford., 89, 680-687.
https://doi.org/10.1016/j.electacta.2012.11.100

Petković BB, Milčić MK, Stanković D, Stambolić I, Manojlović DD, Jovanović VM, Sovilj SP. Complexation ability of octaazamacrocyclic ligand toward Co2+, Ni2+, Cu2+ and Zn2+ metal cations: Experimental and theoretical study. in Electrochimica Acta. 2013;89:680-687.
doi:10.1016/j.electacta.2012.11.100 .

Petković, Branka B., Milčić, Miloš K., Stanković, Dalibor, Stambolić, I., Manojlović, Dragan D., Jovanović, V. M., Sovilj, Sofija P., "Complexation ability of octaazamacrocyclic ligand toward Co2+, Ni2+, Cu2+ and Zn2+ metal cations: Experimental and theoretical study" in Electrochimica Acta, 89 (2013):680-687,
https://doi.org/10.1016/j.electacta.2012.11.100 . .