TY  - JOUR
AU  - Lazović, Mila
AU  - Ivković, Đurđa
AU  - Jankov, Milica
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6444
AB  - Due to the promising applications of propolis as a natural food preservative, functional ingredient or supplement, on one side, and the inherent toxicity and high volatility of organic solvents used for extraction of phenolics from propolis, a range of natural eutectic solvents (NESs) was evaluated for the extraction of phenolic compounds from poplar type propolis to create “green-labelled” food products. The phenolic profile of novel green propolis extracts was evaluated using high-performance thin-layer chromatography, while 16 phenolic compounds were quantified by ultra-high-performance liquid chromatography hyphenated with mass spectrometry. Pinocembrin and chrysin were the most dominant phenolics with an average concentration of 3111 and 3039 mg/kg in lactic acid-based NES, respectively. Biological activity, such as antioxidative and antimicrobial activities, were determined using spectrophotometric and well diffusion methods. Considering all spectrophotometric assays lactic acid-based presented as the best extraction medium for phenolic extraction, with TPC, TFC and RSA values 150.0 mg GAE/g, 190.0 mg RUE/g, and 132.8 mg TE/g on average, respectively. From 38 studied NESs, choline-chloride:tartaric acid (1:1 n/n), glycerol:lactic acid (1:1 n/n) and glycine: lactic acid (1:3 n/n) were the most promising extraction medium for obtaining the propolis extracts enriched with bioactive compounds. Additionally, proposed green solvents were proved to be the designer solvents and showed potential to be tuned to target specific phenolic compounds. Improved, or at least maintained, biological activity of NES propolis extracts support its application in agriculture industry.
PB  - Elsevier
T2  - Food Bioscience
T1  - Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals
VL  - 57
SP  - 103467
DO  - 10.1016/j.fbio.2023.103467
ER  - 

@article{
author = "Lazović, Mila and Ivković, Đurđa and Jankov, Milica and Dimkić, Ivica and Janakiev, Tamara and Trifković, Jelena and Milojković-Opsenica, Dušanka and Ristivojević, Petar",
year = "2024",
abstract = "Due to the promising applications of propolis as a natural food preservative, functional ingredient or supplement, on one side, and the inherent toxicity and high volatility of organic solvents used for extraction of phenolics from propolis, a range of natural eutectic solvents (NESs) was evaluated for the extraction of phenolic compounds from poplar type propolis to create “green-labelled” food products. The phenolic profile of novel green propolis extracts was evaluated using high-performance thin-layer chromatography, while 16 phenolic compounds were quantified by ultra-high-performance liquid chromatography hyphenated with mass spectrometry. Pinocembrin and chrysin were the most dominant phenolics with an average concentration of 3111 and 3039 mg/kg in lactic acid-based NES, respectively. Biological activity, such as antioxidative and antimicrobial activities, were determined using spectrophotometric and well diffusion methods. Considering all spectrophotometric assays lactic acid-based presented as the best extraction medium for phenolic extraction, with TPC, TFC and RSA values 150.0 mg GAE/g, 190.0 mg RUE/g, and 132.8 mg TE/g on average, respectively. From 38 studied NESs, choline-chloride:tartaric acid (1:1 n/n), glycerol:lactic acid (1:1 n/n) and glycine: lactic acid (1:3 n/n) were the most promising extraction medium for obtaining the propolis extracts enriched with bioactive compounds. Additionally, proposed green solvents were proved to be the designer solvents and showed potential to be tuned to target specific phenolic compounds. Improved, or at least maintained, biological activity of NES propolis extracts support its application in agriculture industry.",
publisher = "Elsevier",
journal = "Food Bioscience",
title = "Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals",
volume = "57",
pages = "103467",
doi = "10.1016/j.fbio.2023.103467"
}

Lazović, M., Ivković, Đ., Jankov, M., Dimkić, I., Janakiev, T., Trifković, J., Milojković-Opsenica, D.,& Ristivojević, P.. (2024). Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals. in Food Bioscience
Elsevier., 57, 103467.
https://doi.org/10.1016/j.fbio.2023.103467

Lazović M, Ivković Đ, Jankov M, Dimkić I, Janakiev T, Trifković J, Milojković-Opsenica D, Ristivojević P. Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals. in Food Bioscience. 2024;57:103467.
doi:10.1016/j.fbio.2023.103467 .

Lazović, Mila, Ivković, Đurđa, Jankov, Milica, Dimkić, Ivica, Janakiev, Tamara, Trifković, Jelena, Milojković-Opsenica, Dušanka, Ristivojević, Petar, "Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals" in Food Bioscience, 57 (2024):103467,
https://doi.org/10.1016/j.fbio.2023.103467 . .

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić-Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
SP  - 134184
DO  - 10.1016/j.foodchem.2022.134184
ER  - 

@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić-Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
pages = "134184",
doi = "10.1016/j.foodchem.2022.134184"
}

Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić-Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Elsevier., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184

Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić-Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .

Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić-Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

TY  - DATA
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić-Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184
VL  - 402
SP  - 134184
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5633
ER  - 

@misc{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić-Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184",
volume = "402",
pages = "134184",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5633"
}

Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić-Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry
Elsevier., 402, 134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633

Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić-Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry. 2023;402:134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633 .

Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić-Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184" in Food Chemistry, 402 (2023):134184,
https://hdl.handle.net/21.15107/rcub_cherry_5633 .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5874
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
VL  - 24
IS  - 3
SP  - 1903
DO  - 10.3390/ijms24031903
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study",
volume = "24",
number = "3",
pages = "1903",
doi = "10.3390/ijms24031903"
}

Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences
MDPI., 24(3), 1903.
https://doi.org/10.3390/ijms24031903

Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3):1903.
doi:10.3390/ijms24031903 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023):1903,
https://doi.org/10.3390/ijms24031903 . .

TY  - DATA
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5875
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
VL  - 24
IS  - 3
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5875
ER  - 

@misc{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study",
volume = "24",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5875"
}

Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences
MDPI., 24(3).
https://hdl.handle.net/21.15107/rcub_cherry_5875

Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3).
https://hdl.handle.net/21.15107/rcub_cherry_5875 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5875 .

TY  - JOUR
AU  - Šenk, Milena
AU  - Simić, Milena
AU  - Milojković-Opsenica, Dušanka
AU  - Brankov, Milan
AU  - Tolimir, Miodrag
AU  - Kodranov, Igor D.
AU  - Dragičević, Vesna
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6421
AB  - Climate changes are one of the biggest threats to food security. Sustainable agriculture, focused on eco-friendly practices for highly efficient food production, enables greater resilience and safety. This study experimented on intercropping and bio-fertilizer application as convenient ecological solutions for crop yield stability and quality. The experiment was conducted during 2018 and 2020 with soybean and common millet sown in three sowing patterns: alternating rows, alternating strips 1 (2 rows of soybean + 2 rows of millet), and alternating strips 2 (2 rows of soybean + 4 rows of millet), as well as sole crops (control), with or without a bio-fertilizer Coveron. Grain yield and nutrient grain yield response were calculated through land equivalent ratio (LER) and element-LER (E-LER), while quality was estimated based on the concentration of antioxidants (phytate phosphorus, total phenolic compounds, and yellow pigment) and elements in grains, including potential bio-availability of essential elements. Results revealed LER values to be >1 for all sowing patterns, with the highest one achieved in alternating strips 1 (1.38) together with a greater level of all antioxidants in millet grain. Intercropping significantly enhanced Fe and Mn accumulation in both crops and simultaneously decreased the concentration of potentially toxic elements (Al, Cr) in millet grain. Potential bio-availability of essential elements, expressed through the ratio between phytic acid and Ca, Mg, Fe, and Zn revealed smaller values in intercropped soybean and millet with the bio-fertilizer. The bio-fertilizer also increased the concentration of some micro-elements in millet grain, classifying it as a highly dependent plant to microbial inoculation. Interaction of intercropping and bio-fertilizer was most pronounced for LER, E-LER, and accumulation of Fe and Mn in grains. These results highlighted the benefits of soybean–common millet intercropping, especially in combination with the bio-fertilizer, in light of enhanced land utilization and nutrient absorption, thus increasing the resilience of soybean and millet under dry land conditions and low-input systems toward stability and food security.
T2  - Frontiers in Nutrition
T2  - Frontiers in Nutrition
T1  - Common millet and soybean intercropping with bio-fertilizer as sustainable practice for managing grain yield and quality
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6421
ER  - 

@article{
author = "Šenk, Milena and Simić, Milena and Milojković-Opsenica, Dušanka and Brankov, Milan and Tolimir, Miodrag and Kodranov, Igor D. and Dragičević, Vesna",
year = "2023",
abstract = "Climate changes are one of the biggest threats to food security. Sustainable agriculture, focused on eco-friendly practices for highly efficient food production, enables greater resilience and safety. This study experimented on intercropping and bio-fertilizer application as convenient ecological solutions for crop yield stability and quality. The experiment was conducted during 2018 and 2020 with soybean and common millet sown in three sowing patterns: alternating rows, alternating strips 1 (2 rows of soybean + 2 rows of millet), and alternating strips 2 (2 rows of soybean + 4 rows of millet), as well as sole crops (control), with or without a bio-fertilizer Coveron. Grain yield and nutrient grain yield response were calculated through land equivalent ratio (LER) and element-LER (E-LER), while quality was estimated based on the concentration of antioxidants (phytate phosphorus, total phenolic compounds, and yellow pigment) and elements in grains, including potential bio-availability of essential elements. Results revealed LER values to be >1 for all sowing patterns, with the highest one achieved in alternating strips 1 (1.38) together with a greater level of all antioxidants in millet grain. Intercropping significantly enhanced Fe and Mn accumulation in both crops and simultaneously decreased the concentration of potentially toxic elements (Al, Cr) in millet grain. Potential bio-availability of essential elements, expressed through the ratio between phytic acid and Ca, Mg, Fe, and Zn revealed smaller values in intercropped soybean and millet with the bio-fertilizer. The bio-fertilizer also increased the concentration of some micro-elements in millet grain, classifying it as a highly dependent plant to microbial inoculation. Interaction of intercropping and bio-fertilizer was most pronounced for LER, E-LER, and accumulation of Fe and Mn in grains. These results highlighted the benefits of soybean–common millet intercropping, especially in combination with the bio-fertilizer, in light of enhanced land utilization and nutrient absorption, thus increasing the resilience of soybean and millet under dry land conditions and low-input systems toward stability and food security.",
journal = "Frontiers in Nutrition, Frontiers in Nutrition",
title = "Common millet and soybean intercropping with bio-fertilizer as sustainable practice for managing grain yield and quality",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6421"
}

Šenk, M., Simić, M., Milojković-Opsenica, D., Brankov, M., Tolimir, M., Kodranov, I. D.,& Dragičević, V.. (2023). Common millet and soybean intercropping with bio-fertilizer as sustainable practice for managing grain yield and quality. in Frontiers in Nutrition, 10.
https://hdl.handle.net/21.15107/rcub_cherry_6421

Šenk M, Simić M, Milojković-Opsenica D, Brankov M, Tolimir M, Kodranov ID, Dragičević V. Common millet and soybean intercropping with bio-fertilizer as sustainable practice for managing grain yield and quality. in Frontiers in Nutrition. 2023;10.
https://hdl.handle.net/21.15107/rcub_cherry_6421 .

Šenk, Milena, Simić, Milena, Milojković-Opsenica, Dušanka, Brankov, Milan, Tolimir, Miodrag, Kodranov, Igor D., Dragičević, Vesna, "Common millet and soybean intercropping with bio-fertilizer as sustainable practice for managing grain yield and quality" in Frontiers in Nutrition, 10 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6421 .

TY  - CONF
AU  - Jakanovski, Mihajlo V.
AU  - Horvacki, Nikola M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6226
AB  - Honey is very complex natural substance consisted of sugars, water, and other compounds in smaller quantities such as proteins (enzymes), organic acids, vitamins, minerals, phenolics, and a large variety of volatile compounds. Discriminating the honeys according to botanical and/or geographical origin may be possible according to their characteristic constituents including organic acids profile. These acids are related to the colour and flavour of honey and its chemical properties such as acidity, pH and electrical conductivity. Honey is deceptively acidic, as the high sugar content tends to mask the acidity in the taste. Citric acid among other acids, is present in honey in moderate amount but it's presence could be potentially used as marker for authenticity control [1].

Fraudulent addition of compounds in honey sets a necesity to establish the natural level of the specific substances, and subsequently determine when this level is exceeded, indicating adulteration. Citric acid can be added to decrease pH in adulterated honey where normal pH levels have been exceeded. Also increased citrate concentration in honeys could be a result of addition of invert syrup to honey. Therefore there is a need for a method to determine citrate content in honey quickly and efficiently1. In current work a method for determination of organic acids profile in honey using ion chromatography with conductometric detection (IC-CD) without prior separation of compounds and chemical derivatization was developed. This approach for citric acid determination is characterized by LOD and LOQ values of 0.05 and 0.20 mg/L, respectively. Low RSD Sy/x value and r2 level of 0.9999 indicates a good regression model. Trueness of method was confirmed by good recoveries (108%, 104% and 95% for spiked levels 75%, 100% and 125%, respectively), while precision was confirmed by HorRat value (0.30) less than 2 [2].
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Validation and optimization of ion chromatography based method for citric acid determination in Robinia pseudoacacia honey
SP  - 19
EP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6226
ER  - 

@conference{
author = "Jakanovski, Mihajlo V. and Horvacki, Nikola M. and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Honey is very complex natural substance consisted of sugars, water, and other compounds in smaller quantities such as proteins (enzymes), organic acids, vitamins, minerals, phenolics, and a large variety of volatile compounds. Discriminating the honeys according to botanical and/or geographical origin may be possible according to their characteristic constituents including organic acids profile. These acids are related to the colour and flavour of honey and its chemical properties such as acidity, pH and electrical conductivity. Honey is deceptively acidic, as the high sugar content tends to mask the acidity in the taste. Citric acid among other acids, is present in honey in moderate amount but it's presence could be potentially used as marker for authenticity control [1].

Fraudulent addition of compounds in honey sets a necesity to establish the natural level of the specific substances, and subsequently determine when this level is exceeded, indicating adulteration. Citric acid can be added to decrease pH in adulterated honey where normal pH levels have been exceeded. Also increased citrate concentration in honeys could be a result of addition of invert syrup to honey. Therefore there is a need for a method to determine citrate content in honey quickly and efficiently1. In current work a method for determination of organic acids profile in honey using ion chromatography with conductometric detection (IC-CD) without prior separation of compounds and chemical derivatization was developed. This approach for citric acid determination is characterized by LOD and LOQ values of 0.05 and 0.20 mg/L, respectively. Low RSD Sy/x value and r2 level of 0.9999 indicates a good regression model. Trueness of method was confirmed by good recoveries (108%, 104% and 95% for spiked levels 75%, 100% and 125%, respectively), while precision was confirmed by HorRat value (0.30) less than 2 [2].",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Validation and optimization of ion chromatography based method for citric acid determination in Robinia pseudoacacia honey",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6226"
}

Jakanovski, M. V., Horvacki, N. M.,& Milojković-Opsenica, D.. (2023). Validation and optimization of ion chromatography based method for citric acid determination in Robinia pseudoacacia honey. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
Belgrade : Serbian Chemical Society., 19-19.
https://hdl.handle.net/21.15107/rcub_cherry_6226

Jakanovski MV, Horvacki NM, Milojković-Opsenica D. Validation and optimization of ion chromatography based method for citric acid determination in Robinia pseudoacacia honey. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;:19-19.
https://hdl.handle.net/21.15107/rcub_cherry_6226 .

Jakanovski, Mihajlo V., Horvacki, Nikola M., Milojković-Opsenica, Dušanka, "Validation and optimization of ion chromatography based method for citric acid determination in Robinia pseudoacacia honey" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023):19-19,
https://hdl.handle.net/21.15107/rcub_cherry_6226 .

TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Đokić, Ivana
AU  - Tosti, Tomislav
AU  - Popović, Slađana
AU  - Milojković-Opsenica, Dušanka
AU  - Vukojević, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6385
AB  - The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main challenges in fungal pretreatment of lignocellulosic feedstock.
PB  - Cellulose Chemistry and Technology
T2  - Cellulose Chemistry and Technology
T1  - WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS
VL  - 57
IS  - 7-8
SP  - 815
EP  - 828
DO  - 10.35812/CelluloseChemTechnol.2023.57.72
ER  - 

@article{
author = "Knežević, Aleksandar and Đokić, Ivana and Tosti, Tomislav and Popović, Slađana and Milojković-Opsenica, Dušanka and Vukojević, Jelena",
year = "2023",
abstract = "The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main challenges in fungal pretreatment of lignocellulosic feedstock.",
publisher = "Cellulose Chemistry and Technology",
journal = "Cellulose Chemistry and Technology",
title = "WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS",
volume = "57",
number = "7-8",
pages = "815-828",
doi = "10.35812/CelluloseChemTechnol.2023.57.72"
}

Knežević, A., Đokić, I., Tosti, T., Popović, S., Milojković-Opsenica, D.,& Vukojević, J.. (2023). WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS. in Cellulose Chemistry and Technology
Cellulose Chemistry and Technology., 57(7-8), 815-828.
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72

Knežević A, Đokić I, Tosti T, Popović S, Milojković-Opsenica D, Vukojević J. WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS. in Cellulose Chemistry and Technology. 2023;57(7-8):815-828.
doi:10.35812/CelluloseChemTechnol.2023.57.72 .

Knežević, Aleksandar, Đokić, Ivana, Tosti, Tomislav, Popović, Slađana, Milojković-Opsenica, Dušanka, Vukojević, Jelena, "WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS" in Cellulose Chemistry and Technology, 57, no. 7-8 (2023):815-828,
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72 . .

TY  - JOUR
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5876
AB  - The aim of this study is to develop a rapid, effect-directed screening method for quality assessment of bee pollen-honey mixtures. The comparative antioxidant potential and phenolic content of honey, bee pollen, and the bee pollen-honey mixtures, was performed using spectrophotometry. The total phenolic content and antioxidative activity of bee pollen-honey mixtures with 20 % bee pollen share were in the range 3.03–3.11 mg GAE/g, and 6.02–6.96 mmol TE/kg, respectively, while mixtures with 30 % bee pollen share contained 3.92–4.18 mg GAE/g, and 9.69–10.11 mmol TE/kg. Chromatographic fingerprint of bee pollen-honey mixtures was performed by high-performance thin-layer chromatography with conditions developed by authors and reported for the first time. Fingerprint analysis hyphenated with chemometrics enabled authenticity assessments of honey in mixtures. Results indicate that bee pollen-honey mixtures represent a food with highly, both, nutritious characteristics and health-promoting effect.
PB  - Wiley
T2  - Chemistry & Biodiversity
T2  - Chemistry & Biodiversity
T1  - Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics
VL  - 20
IS  - 3
SP  - e202201141
DO  - 10.1002/cbdv.202201141
ER  - 

@article{
author = "Mosić, Mirjana D. and Trifković, Jelena and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "The aim of this study is to develop a rapid, effect-directed screening method for quality assessment of bee pollen-honey mixtures. The comparative antioxidant potential and phenolic content of honey, bee pollen, and the bee pollen-honey mixtures, was performed using spectrophotometry. The total phenolic content and antioxidative activity of bee pollen-honey mixtures with 20 % bee pollen share were in the range 3.03–3.11 mg GAE/g, and 6.02–6.96 mmol TE/kg, respectively, while mixtures with 30 % bee pollen share contained 3.92–4.18 mg GAE/g, and 9.69–10.11 mmol TE/kg. Chromatographic fingerprint of bee pollen-honey mixtures was performed by high-performance thin-layer chromatography with conditions developed by authors and reported for the first time. Fingerprint analysis hyphenated with chemometrics enabled authenticity assessments of honey in mixtures. Results indicate that bee pollen-honey mixtures represent a food with highly, both, nutritious characteristics and health-promoting effect.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity, Chemistry & Biodiversity",
title = "Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics",
volume = "20",
number = "3",
pages = "e202201141",
doi = "10.1002/cbdv.202201141"
}

Mosić, M. D., Trifković, J., Ristivojević, P.,& Milojković-Opsenica, D.. (2023). Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics. in Chemistry & Biodiversity
Wiley., 20(3), e202201141.
https://doi.org/10.1002/cbdv.202201141

Mosić MD, Trifković J, Ristivojević P, Milojković-Opsenica D. Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics. in Chemistry & Biodiversity. 2023;20(3):e202201141.
doi:10.1002/cbdv.202201141 .

Mosić, Mirjana D., Trifković, Jelena, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics" in Chemistry & Biodiversity, 20, no. 3 (2023):e202201141,
https://doi.org/10.1002/cbdv.202201141 . .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Nešić, Jelena
AU  - Andrić, Filip
AU  - Nedić, Nebojša
AU  - Stanisavljević, Ljubiša
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5895
AB  - Propolis is a resinous natural substance collected by honeybees from different plant sources. The element content of propolis is influenced by the content of elements in the soil, climatic factors, and the degree of pollution. The aim of the study was to characterize element composition and content of toxic elements in 51 propolis collected from different locations in Serbia and to evaluate the differences among them. Determination of the 21 elements were performed using Inductively coupled plasma - optical emission spectrometry. K was the most abundant element, followed by Ca, Mg, Si, Fe, Zn, and Al. The microelements B, Ba, Mn, Na, Pb, and Ti were also present in propolis, while Co, Cr, Cu, Mo, Ni, Sr, and V were found in trace. Toxic elements such as Pb, As, and Cd were found in the trace. Kruskal-Wallis tests differentiate groups of samples by geographical origin. The presence of B, Fe, Sr, Ti, Zn, and As demonstrated statistical significance between six regions of Serbia.
PB  - Wiley
T2  - Chemistry & Biodiversity
T2  - Chemistry & Biodiversity
T1  - Elemental Profile of Propolis from Different Areas of Serbia
VL  - 20
IS  - 3
SP  - e202201140
DO  - 10.1002/cbdv.202201140
ER  - 

@article{
author = "Ristivojević, Petar and Nešić, Jelena and Andrić, Filip and Nedić, Nebojša and Stanisavljević, Ljubiša and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Propolis is a resinous natural substance collected by honeybees from different plant sources. The element content of propolis is influenced by the content of elements in the soil, climatic factors, and the degree of pollution. The aim of the study was to characterize element composition and content of toxic elements in 51 propolis collected from different locations in Serbia and to evaluate the differences among them. Determination of the 21 elements were performed using Inductively coupled plasma - optical emission spectrometry. K was the most abundant element, followed by Ca, Mg, Si, Fe, Zn, and Al. The microelements B, Ba, Mn, Na, Pb, and Ti were also present in propolis, while Co, Cr, Cu, Mo, Ni, Sr, and V were found in trace. Toxic elements such as Pb, As, and Cd were found in the trace. Kruskal-Wallis tests differentiate groups of samples by geographical origin. The presence of B, Fe, Sr, Ti, Zn, and As demonstrated statistical significance between six regions of Serbia.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity, Chemistry & Biodiversity",
title = "Elemental Profile of Propolis from Different Areas of Serbia",
volume = "20",
number = "3",
pages = "e202201140",
doi = "10.1002/cbdv.202201140"
}

Ristivojević, P., Nešić, J., Andrić, F., Nedić, N., Stanisavljević, L., Milojković-Opsenica, D.,& Trifković, J.. (2023). Elemental Profile of Propolis from Different Areas of Serbia. in Chemistry & Biodiversity
Wiley., 20(3), e202201140.
https://doi.org/10.1002/cbdv.202201140

Ristivojević P, Nešić J, Andrić F, Nedić N, Stanisavljević L, Milojković-Opsenica D, Trifković J. Elemental Profile of Propolis from Different Areas of Serbia. in Chemistry & Biodiversity. 2023;20(3):e202201140.
doi:10.1002/cbdv.202201140 .

Ristivojević, Petar, Nešić, Jelena, Andrić, Filip, Nedić, Nebojša, Stanisavljević, Ljubiša, Milojković-Opsenica, Dušanka, Trifković, Jelena, "Elemental Profile of Propolis from Different Areas of Serbia" in Chemistry & Biodiversity, 20, no. 3 (2023):e202201140,
https://doi.org/10.1002/cbdv.202201140 . .

TY  - JOUR
AU  - Jankov, Milica
AU  - Ristivojević, Petar
AU  - Cvijetić, Ilija
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6232
AB  - High-Performance Thin-Layer Chromatography (HPTLC)-radical scavenging capacity (RSC) assays are stan- dard techniques for the separation and identification of antioxidants from complex mixtures. HPTLC coupled with DPPH · visualization of chromatograms allows for the detection of individual antioxidants. However, other HPTLC-RSC assays that recognize compounds exhibiting different mechanisms of radical- scavenging activity are rarely reported. In this study, we developed an integrated approach that combines five HPTLC-RSC assays, principal component analysis (PCA) and quantum chemical calculations to assess the antioxidant capacity of Sempervivum tectorum L. leaf extracts. Two HPTLC assays - potassium hexa- cyanoferrate(III) total reducing power assay (TRP) and total antioxidant capacity by phosphomolybdenum method (TAC) - were developed for the first time. The method supports a more in-depth study of the RSC of natural products, as it compares the radical scavenging fingerprints of S. tectorum leaf extracts and recognizes differences in their individual bioactive constituents. Kaempferol, kaempferol 3- O -glucoside, quercetin 3- O -glucoside, caffeic acid, and gallic acid were identified as the compounds that discriminate HPTLC-RSC assays according to their mechanism of action and capture the similarities between 20 S. tec- torum samples. Additionally, DFT calculations on M06–2X/6–31 + G (d,p) level were applied to map ther- modynamic feasibility of hydrogen atom transfer (HAT) and single electron transfer (SET) mechanisms of the identified compounds. Based on experimental and theoretical results, a combination of HPTLC-ABTS and HPTLC-TAC assays were proposed as the optimal method for mapping the antioxidants from S. tec- torum . This study represents a step forward in identifying and quantifying individual antioxidants from complex food and natural product matrices in a more rational manner.
PB  - Elsevier
T2  - Journal of Chromatography A
T1  - Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach
VL  - 1703
SP  - 464082
DO  - 10.1016/j.chroma.2023.464082
ER  - 

@article{
author = "Jankov, Milica and Ristivojević, Petar and Cvijetić, Ilija and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "High-Performance Thin-Layer Chromatography (HPTLC)-radical scavenging capacity (RSC) assays are stan- dard techniques for the separation and identification of antioxidants from complex mixtures. HPTLC coupled with DPPH · visualization of chromatograms allows for the detection of individual antioxidants. However, other HPTLC-RSC assays that recognize compounds exhibiting different mechanisms of radical- scavenging activity are rarely reported. In this study, we developed an integrated approach that combines five HPTLC-RSC assays, principal component analysis (PCA) and quantum chemical calculations to assess the antioxidant capacity of Sempervivum tectorum L. leaf extracts. Two HPTLC assays - potassium hexa- cyanoferrate(III) total reducing power assay (TRP) and total antioxidant capacity by phosphomolybdenum method (TAC) - were developed for the first time. The method supports a more in-depth study of the RSC of natural products, as it compares the radical scavenging fingerprints of S. tectorum leaf extracts and recognizes differences in their individual bioactive constituents. Kaempferol, kaempferol 3- O -glucoside, quercetin 3- O -glucoside, caffeic acid, and gallic acid were identified as the compounds that discriminate HPTLC-RSC assays according to their mechanism of action and capture the similarities between 20 S. tec- torum samples. Additionally, DFT calculations on M06–2X/6–31 + G (d,p) level were applied to map ther- modynamic feasibility of hydrogen atom transfer (HAT) and single electron transfer (SET) mechanisms of the identified compounds. Based on experimental and theoretical results, a combination of HPTLC-ABTS and HPTLC-TAC assays were proposed as the optimal method for mapping the antioxidants from S. tec- torum . This study represents a step forward in identifying and quantifying individual antioxidants from complex food and natural product matrices in a more rational manner.",
publisher = "Elsevier",
journal = "Journal of Chromatography A",
title = "Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach",
volume = "1703",
pages = "464082",
doi = "10.1016/j.chroma.2023.464082"
}

Jankov, M., Ristivojević, P., Cvijetić, I.,& Milojković-Opsenica, D.. (2023). Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach. in Journal of Chromatography A
Elsevier., 1703, 464082.
https://doi.org/10.1016/j.chroma.2023.464082

Jankov M, Ristivojević P, Cvijetić I, Milojković-Opsenica D. Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach. in Journal of Chromatography A. 2023;1703:464082.
doi:10.1016/j.chroma.2023.464082 .

Jankov, Milica, Ristivojević, Petar, Cvijetić, Ilija, Milojković-Opsenica, Dušanka, "Assessing radical scavenging capacity of Sempervivum tectorum L. leaf extracts: An integrated high-performance thin-layer chromatography/ in silico /chemometrics approach" in Journal of Chromatography A, 1703 (2023):464082,
https://doi.org/10.1016/j.chroma.2023.464082 . .

TY  - CONF
AU  - Jankov, Milica
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6209
AB  - Sempervivum tectorum L. is a perennial plant with succulent leaves arranged in a rosette. Despite its wide use in traditional medicine for the treatment of various changes on the skin, nervousness, anxiety, menstrual ailments, etc., it still does not have a biography in the European Pharmacopoeia¹. The antibacterial effect of juice squeezed from the leaves and tea made from dried leaves was tested against bacteria that cause inflammation of the ear and bladder². Direct bioautography on HPTLC plates for the investigation of antibacterial activity of the houseleek leaves extracts was done for the first time. Four HPTLC bioassays were developed for gram-positive bacteria (B. subtilis, M. lysodeikticus, MRSA, and S. aureus), and two for gramnegative bacteria (E. coli and K. pneumoniae). Antibacterial activity of grampositive bacteria comes from active compounds at RF 0.57 and 0.92. HPTLC bioassays show higher sensitivity for gram-negative bacteria, as numerous active bands were observed at RF 0.03, 0.38, 0.42, 0.56, 0.57, 0.86 and 0.92. By applying chemometrics and principal components analysis, the HPTLC bioassays of grampositive bacteria were separated into two groups on the scores plot graphic along PC1 axis. The bioassays for B. subtilis and M. lysodeikticus form a less uniform group in the upper left quadrant, while the bioassays for MRSA and S. aureus form a homogeneous group in the lower right quadrant. Presence or absence of the active bands at RF 0.57 and 0.92 affect these separations. The bioassays for the two gramnegative bacteria are separated along both axes on the scores plot graphic from each other but also from the other bioassays for the gram-positive bacteria. This separation is influenced by the presence of numerous active bands. For further investigation it is necessary to identify compounds responsible for the antibacterial activity of the houseleek leaves extracts.
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6209
ER  - 

@conference{
author = "Jankov, Milica and Milojković-Opsenica, Dušanka and Ristivojević, Petar",
year = "2023",
abstract = "Sempervivum tectorum L. is a perennial plant with succulent leaves arranged in a rosette. Despite its wide use in traditional medicine for the treatment of various changes on the skin, nervousness, anxiety, menstrual ailments, etc., it still does not have a biography in the European Pharmacopoeia¹. The antibacterial effect of juice squeezed from the leaves and tea made from dried leaves was tested against bacteria that cause inflammation of the ear and bladder². Direct bioautography on HPTLC plates for the investigation of antibacterial activity of the houseleek leaves extracts was done for the first time. Four HPTLC bioassays were developed for gram-positive bacteria (B. subtilis, M. lysodeikticus, MRSA, and S. aureus), and two for gramnegative bacteria (E. coli and K. pneumoniae). Antibacterial activity of grampositive bacteria comes from active compounds at RF 0.57 and 0.92. HPTLC bioassays show higher sensitivity for gram-negative bacteria, as numerous active bands were observed at RF 0.03, 0.38, 0.42, 0.56, 0.57, 0.86 and 0.92. By applying chemometrics and principal components analysis, the HPTLC bioassays of grampositive bacteria were separated into two groups on the scores plot graphic along PC1 axis. The bioassays for B. subtilis and M. lysodeikticus form a less uniform group in the upper left quadrant, while the bioassays for MRSA and S. aureus form a homogeneous group in the lower right quadrant. Presence or absence of the active bands at RF 0.57 and 0.92 affect these separations. The bioassays for the two gramnegative bacteria are separated along both axes on the scores plot graphic from each other but also from the other bioassays for the gram-positive bacteria. This separation is influenced by the presence of numerous active bands. For further investigation it is necessary to identify compounds responsible for the antibacterial activity of the houseleek leaves extracts.",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6209"
}

Jankov, M., Milojković-Opsenica, D.,& Ristivojević, P.. (2023). Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science.
https://hdl.handle.net/21.15107/rcub_cherry_6209

Jankov M, Milojković-Opsenica D, Ristivojević P. Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6209 .

Jankov, Milica, Milojković-Opsenica, Dušanka, Ristivojević, Petar, "Assessment of antibacterial activity of Sempervivum tectorum L. leaves extracts using HPTLC bioassays and chemometrics" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6209 .

TY  - JOUR
AU  - Lazović, Mila
AU  - Cvijetić, Ilija
AU  - Jankov, Milica
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6230
AB  - Natural Eutectic Solvents (NES) are a promising class of environmentally friendly liquids that offer an alternative to conventional organic solvents. To investigate their potential for extracting phenolic compounds from plant materials, we employed the COSMO-RS (COnductor like Screening MOdel for Real Solvents) computational method. Nineteen NES solvents were prepared to determine the most selective and effective solvent for extracting phenolic compounds from Teucrium chamaedrys using both theoretical and experimental evaluation. Screening of extraction efficiency was performed by quantification of individual compounds using ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS), as well as spectrophotometric assays (total phenolic content, total flavonoid content, and radical scavenging activity). In this paper, we propose a new approach to model NES properties using COSMO-RS. While the majority of previous studies have modeled these solvents as individual or pseudo-component complexes between hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA), we have developed a ternary HBD:HBA:water supramolecular complex model to represent NES solution structures more accurately. This model provides insights into the intermolecular interactions driving the extraction process and predicts extraction efficiencies that agree with experimental data. Two eutectic mixtures (choline chloride:succinic acid in molar ratio 1:1 and 20% of water (w/w) and choline chloride: glycerol in molar ratio 1:1 and 20% of water (w/w)) showed strong affinity towards phenolic compounds. Overall, our findings suggest that the ternary complex model is a more appropriate approach for modeling NES properties.
PB  - Elsevier
T2  - Journal of Molecular Liquids
T1  - COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys
VL  - 387
SP  - 122649
DO  - 10.1016/j.molliq.2023.122649
ER  - 

@article{
author = "Lazović, Mila and Cvijetić, Ilija and Jankov, Milica and Milojković-Opsenica, Dušanka and Trifković, Jelena and Ristivojević, Petar",
year = "2023",
abstract = "Natural Eutectic Solvents (NES) are a promising class of environmentally friendly liquids that offer an alternative to conventional organic solvents. To investigate their potential for extracting phenolic compounds from plant materials, we employed the COSMO-RS (COnductor like Screening MOdel for Real Solvents) computational method. Nineteen NES solvents were prepared to determine the most selective and effective solvent for extracting phenolic compounds from Teucrium chamaedrys using both theoretical and experimental evaluation. Screening of extraction efficiency was performed by quantification of individual compounds using ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS), as well as spectrophotometric assays (total phenolic content, total flavonoid content, and radical scavenging activity). In this paper, we propose a new approach to model NES properties using COSMO-RS. While the majority of previous studies have modeled these solvents as individual or pseudo-component complexes between hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA), we have developed a ternary HBD:HBA:water supramolecular complex model to represent NES solution structures more accurately. This model provides insights into the intermolecular interactions driving the extraction process and predicts extraction efficiencies that agree with experimental data. Two eutectic mixtures (choline chloride:succinic acid in molar ratio 1:1 and 20% of water (w/w) and choline chloride: glycerol in molar ratio 1:1 and 20% of water (w/w)) showed strong affinity towards phenolic compounds. Overall, our findings suggest that the ternary complex model is a more appropriate approach for modeling NES properties.",
publisher = "Elsevier",
journal = "Journal of Molecular Liquids",
title = "COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys",
volume = "387",
pages = "122649",
doi = "10.1016/j.molliq.2023.122649"
}

Lazović, M., Cvijetić, I., Jankov, M., Milojković-Opsenica, D., Trifković, J.,& Ristivojević, P.. (2023). COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys. in Journal of Molecular Liquids
Elsevier., 387, 122649.
https://doi.org/10.1016/j.molliq.2023.122649

Lazović M, Cvijetić I, Jankov M, Milojković-Opsenica D, Trifković J, Ristivojević P. COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys. in Journal of Molecular Liquids. 2023;387:122649.
doi:10.1016/j.molliq.2023.122649 .

Lazović, Mila, Cvijetić, Ilija, Jankov, Milica, Milojković-Opsenica, Dušanka, Trifković, Jelena, Ristivojević, Petar, "COSMO-RS in prescreening of Natural Eutectic Solvents for phenolic extraction from Teucrium chamaedrys" in Journal of Molecular Liquids, 387 (2023):122649,
https://doi.org/10.1016/j.molliq.2023.122649 . .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Jović, Marko D.
AU  - Dimkić, Ivica Z.
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
AU  - Trifković, Jelena 
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6220
AB  - Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.
C3  - XXII Congress EuroFoodChem, Book of abstracts
T1  - Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6220
ER  - 

@conference{
author = "Lazović, Mila Č. and Jović, Marko D. and Dimkić, Ivica Z. and Milojković-Opsenica, Dušanka and Ristivojević, Petar and Trifković, Jelena ",
year = "2023",
abstract = "Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.",
journal = "XXII Congress EuroFoodChem, Book of abstracts",
title = "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6220"
}

Lazović, M. Č., Jović, M. D., Dimkić, I. Z., Milojković-Opsenica, D., Ristivojević, P.,& Trifković, J.. (2023). Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts.
https://hdl.handle.net/21.15107/rcub_cherry_6220

Lazović MČ, Jović MD, Dimkić IZ, Milojković-Opsenica D, Ristivojević P, Trifković J. Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

Lazović, Mila Č., Jović, Marko D., Dimkić, Ivica Z., Milojković-Opsenica, Dušanka, Ristivojević, Petar, Trifković, Jelena , "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries" in XXII Congress EuroFoodChem, Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

TY  - CONF
AU  - Jakanovski, Mihajlo V.
AU  - Dramićanin, Aleksandra M.
AU  - Horvacki, Nikola
AU  - Trifković, Jelena
AU  - Rašić, Dubravka
AU  - Brčić Karačonji, Irena
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5933
AB  - Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products
SP  - 73
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5933
ER  - 

@conference{
author = "Jakanovski, Mihajlo V. and Dramićanin, Aleksandra M. and Horvacki, Nikola and Trifković, Jelena and Rašić, Dubravka and Brčić Karačonji, Irena and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products",
pages = "73-74",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5933"
}

Jakanovski, M. V., Dramićanin, A. M., Horvacki, N., Trifković, J., Rašić, D., Brčić Karačonji, I.,& Milojković-Opsenica, D.. (2023). Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933

Jakanovski MV, Dramićanin AM, Horvacki N, Trifković J, Rašić D, Brčić Karačonji I, Milojković-Opsenica D. Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

Jakanovski, Mihajlo V., Dramićanin, Aleksandra M., Horvacki, Nikola, Trifković, Jelena, Rašić, Dubravka, Brčić Karačonji, Irena, Milojković-Opsenica, Dušanka, "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):73-74,
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

TY  - CONF
AU  - Horvacki, Nikola M.
AU  - Jakanovski, Mihajlo V.
AU  - Milojković-Opsenica, Dušanka
AU  - Fotirić Akšić, Milica
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6227
AB  - Soluble sugars and organic acids have a strong impact on the overall sensory quality of fruits. Organic acids also facilitate in stabilization of anthocyanins, and they extend the shelf-life of fresh fruits and their processed products. The production of new apple-based products with health beneficial properties is leading to agronomical and chemical re-evaluation of old apple cultivars.
In this study the mesocarp samples of five conventional, five resistant and fifteen autochthonous apple cultivars from a single growing season were deliberated. Assessment was based on contents of four sugars, one sugar alcohol and five organic acids that were determined by the usage of HPAEC-PAD and HPAEC-CD systems. Fructose was detected as dominant sugar in all cases (50.4 – 77.9 mg/kg FW) and malic acid as dominant acid (1.1 – 4.5 mg/kg FW). Regarding the average values, xylose and sorbitol were in higher amount in autochthonous cultivars compared to others. In resistant cultivars shikimate and malate were found in lower concentrations compared to other cultivar types. Among the obtained data, highest correlation values were found between citrate and isocitrate, and also between citrate and malate (0.905 and 0.839, respectively).
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Comparative assessment of preeminent sugars and organic acids in fruits of several apple cultivars
SP  - 20
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6227
ER  - 

@conference{
author = "Horvacki, Nikola M. and Jakanovski, Mihajlo V. and Milojković-Opsenica, Dušanka and Fotirić Akšić, Milica",
year = "2023",
abstract = "Soluble sugars and organic acids have a strong impact on the overall sensory quality of fruits. Organic acids also facilitate in stabilization of anthocyanins, and they extend the shelf-life of fresh fruits and their processed products. The production of new apple-based products with health beneficial properties is leading to agronomical and chemical re-evaluation of old apple cultivars.
In this study the mesocarp samples of five conventional, five resistant and fifteen autochthonous apple cultivars from a single growing season were deliberated. Assessment was based on contents of four sugars, one sugar alcohol and five organic acids that were determined by the usage of HPAEC-PAD and HPAEC-CD systems. Fructose was detected as dominant sugar in all cases (50.4 – 77.9 mg/kg FW) and malic acid as dominant acid (1.1 – 4.5 mg/kg FW). Regarding the average values, xylose and sorbitol were in higher amount in autochthonous cultivars compared to others. In resistant cultivars shikimate and malate were found in lower concentrations compared to other cultivar types. Among the obtained data, highest correlation values were found between citrate and isocitrate, and also between citrate and malate (0.905 and 0.839, respectively).",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Comparative assessment of preeminent sugars and organic acids in fruits of several apple cultivars",
pages = "20-20",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6227"
}

Horvacki, N. M., Jakanovski, M. V., Milojković-Opsenica, D.,& Fotirić Akšić, M.. (2023). Comparative assessment of preeminent sugars and organic acids in fruits of several apple cultivars. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
Belgrade : Serbian Chemical Society., 20-20.
https://hdl.handle.net/21.15107/rcub_cherry_6227

Horvacki NM, Jakanovski MV, Milojković-Opsenica D, Fotirić Akšić M. Comparative assessment of preeminent sugars and organic acids in fruits of several apple cultivars. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;:20-20.
https://hdl.handle.net/21.15107/rcub_cherry_6227 .

Horvacki, Nikola M., Jakanovski, Mihajlo V., Milojković-Opsenica, Dušanka, Fotirić Akšić, Milica, "Comparative assessment of preeminent sugars and organic acids in fruits of several apple cultivars" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023):20-20,
https://hdl.handle.net/21.15107/rcub_cherry_6227 .

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6229
AB  - Strawberry (Fragaria x ananassa Duch.) is widely known for its nutritional value, richness in polyphenols and antioxidant capacity. In abundance of numerous ongoing strawberry breeding programs worldwide, some of the main parameters for breeding and selection are fruit quality, flavour and aroma which are directly affecting commercial acceptance [1]. These could be easily identified with some of objective measurements such as soluble solids concentration (°Brix), acid contents (titratable acidity, TA) and Brix/acid ratio, which could be strong predictors of quality in terms of consumer acceptability [1,2]. In this trial, we compared 24 strawberry genotypes, including 12 parental varieties and 12 perspective candidates obtained by crossing these particular parental varieties. Results of °Brix, titratable acidity and °Brix/acid ratio measurements indicated that new perspective candidates have had higher °Brix/acid ratio than the parental varieties, leading to conclusion that those genotypes had better flavour. Therefore, this ratio could potentially be used as indicator for distinguishing consumer acceptance.
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6229
ER  - 

@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Strawberry (Fragaria x ananassa Duch.) is widely known for its nutritional value, richness in polyphenols and antioxidant capacity. In abundance of numerous ongoing strawberry breeding programs worldwide, some of the main parameters for breeding and selection are fruit quality, flavour and aroma which are directly affecting commercial acceptance [1]. These could be easily identified with some of objective measurements such as soluble solids concentration (°Brix), acid contents (titratable acidity, TA) and Brix/acid ratio, which could be strong predictors of quality in terms of consumer acceptability [1,2]. In this trial, we compared 24 strawberry genotypes, including 12 parental varieties and 12 perspective candidates obtained by crossing these particular parental varieties. Results of °Brix, titratable acidity and °Brix/acid ratio measurements indicated that new perspective candidates have had higher °Brix/acid ratio than the parental varieties, leading to conclusion that those genotypes had better flavour. Therefore, this ratio could potentially be used as indicator for distinguishing consumer acceptance.",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6229"
}

Momirović, N., Nikolić, D., Jakanovski, M. V.,& Milojković-Opsenica, D.. (2023). Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
Belgrade : Serbian Chemical Society..
https://hdl.handle.net/21.15107/rcub_cherry_6229

Momirović N, Nikolić D, Jakanovski MV, Milojković-Opsenica D. Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6229 .

Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Milojković-Opsenica, Dušanka, "Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6229 .

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6228
AB  - Strawberry (Fragaria x ananassa Duch.) is widely known for its nutritional value, richness in polyphenols and antioxidant capacity. In abundance of numerous ongoing strawberry breeding programs worldwide, some of the main parameters for breeding and selection are fruit quality, flavour and aroma which are directly affecting commercial acceptance [1]. These could be easily identified with some of objective measurements such as soluble solids concentration (°Brix), acid contents (titratable acidity, TA) and Brix/acid ratio, which could be strong predictors of quality in terms of consumer acceptability [1,2]. In this trial, we compared 24 strawberry genotypes, including 12 parental varieties and 12 perspective candidates obtained by crossing these particular parental varieties. Results of °Brix, titratable acidity and °Brix/acid ratio measurements indicated that new perspective candidates have had higher °Brix/acid ratio than the parental varieties, leading to conclusion that those genotypes had better flavour. Therefore, this ratio could potentially be used as indicator for distinguishing consumer acceptance.
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio
SP  - 126
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6228
ER  - 

@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Strawberry (Fragaria x ananassa Duch.) is widely known for its nutritional value, richness in polyphenols and antioxidant capacity. In abundance of numerous ongoing strawberry breeding programs worldwide, some of the main parameters for breeding and selection are fruit quality, flavour and aroma which are directly affecting commercial acceptance [1]. These could be easily identified with some of objective measurements such as soluble solids concentration (°Brix), acid contents (titratable acidity, TA) and Brix/acid ratio, which could be strong predictors of quality in terms of consumer acceptability [1,2]. In this trial, we compared 24 strawberry genotypes, including 12 parental varieties and 12 perspective candidates obtained by crossing these particular parental varieties. Results of °Brix, titratable acidity and °Brix/acid ratio measurements indicated that new perspective candidates have had higher °Brix/acid ratio than the parental varieties, leading to conclusion that those genotypes had better flavour. Therefore, this ratio could potentially be used as indicator for distinguishing consumer acceptance.",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6228"
}

Momirović, N., Nikolić, D., Jakanovski, M. V.,& Milojković-Opsenica, D.. (2023). Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
Belgrade : Serbian Chemical Society., 126-126.
https://hdl.handle.net/21.15107/rcub_cherry_6228

Momirović N, Nikolić D, Jakanovski MV, Milojković-Opsenica D. Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;:126-126.
https://hdl.handle.net/21.15107/rcub_cherry_6228 .

Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Milojković-Opsenica, Dušanka, "Robust predictor for consumer’s acceptance of newly bred strawberry varieties based on Brix/acidity ratio" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023):126-126,
https://hdl.handle.net/21.15107/rcub_cherry_6228 .

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Drami, Aleksandra
AU  - Mosić, Mirjana
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5974
AB  - Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).

Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.

Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.

References:
[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).
[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process
SP  - 175
EP  - 175
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5974
ER  - 

@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Drami, Aleksandra and Mosić, Mirjana and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).

Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.

Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.

References:
[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).
[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process",
pages = "175-175",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5974"
}

Momirović, N., Nikolić, D., Jakanovski, M. V., Drami, A., Mosić, M., Momirović, N.,& Milojković-Opsenica, D.. (2023). The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 175-175.
https://hdl.handle.net/21.15107/rcub_cherry_5974

Momirović N, Nikolić D, Jakanovski MV, Drami A, Mosić M, Momirović N, Milojković-Opsenica D. The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:175-175.
https://hdl.handle.net/21.15107/rcub_cherry_5974 .

Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Drami, Aleksandra, Mosić, Mirjana, Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):175-175,
https://hdl.handle.net/21.15107/rcub_cherry_5974 .

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Drami, Aleksandra
AU  - Mosić, Mirjana
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5975
AB  - Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.References:[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5975
ER  - 

@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Drami, Aleksandra and Mosić, Mirjana and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.References:[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5975"
}

Momirović, N., Nikolić, D., Jakanovski, M. V., Drami, A., Mosić, M., Momirović, N.,& Milojković-Opsenica, D.. (2023). The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_cherry_5975

Momirović N, Nikolić D, Jakanovski MV, Drami A, Mosić M, Momirović N, Milojković-Opsenica D. The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_5975 .

Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Drami, Aleksandra, Mosić, Mirjana, Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5975 .

TY  - CONF
AU  - Cvijanović, Vojin
AU  - Dramićanin, Aleksandra
AU  - Sarić, Beka
AU  - Jakanovski, Mihajlo V.
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5976
AB  - Interest in production of protected crops has grown significantly over the past decade. Different cultivation systems (conventional, integral, and organic) affect the biological activity, which is ultimately reflected in the high quality of the fruits of different vegetable crops. In accordance with the requirements for health-safe products without residues of harmful substances in fruits, there is an increasing need for the production of tomatoes in integral and organic production systems. One of the most important features of these vegetables is their high quality and health safety, high nutritional and biological value. Agricultural production systems and growing practices are critical factors in determining the nutritional quality of tomato fruits [1]. In tomato, the sugar content is one of the important factors and one of the most significant parameters from the aspect of food quality [1]. Therefore, the aim of this work is to monitor fluctuations in sugars content, as parameters
that determine the nutritional value in tomato cultivars, induced by growing under integral and organic conditions. For this purpose, a set of sixteen samples of four types of tomatoes - beef, grapolo, mini and midi plum, and cherry - was analyzed. Each type of tomato included two varieties, grown in two agricultural systems - integral and organic. The sugars profile was obtained using High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAECPAD). The content of eleven sugar components was determined. Fructose and glucose were the major sugar compounds [2], while the sugar microcomponents were trehalose, arabinose, melibiose, sucrose, isomaltose, gentiobiose, raffinose, maltose, and panose. The results showed differences between samples produced in integral and organic growing systems, primarily in microsugar components. Higher content of trehalose and melibiose was found in samples obtained from organic production.

Fig.1. Principal Component Analysis (A)- The difference between integral (I) and organic (O) type of production; score plot (1a) – tomato samples: integral samples (I) 9-14, organic samples (O) 1-8, and loading plot (1b), and results of the Mann-Whitney U test (B) - sugar markers of type of production.

Acknowledgments: The authors would like to thank the Zeleni hit d.o.o. from Belgrade, Serbia, for field trials and support. This work has been supported by the Ministry of Science, Technological 
Development and Innovation of Republic of Serbia, Contract number: 451-03- 47/2023-01/200168, 451-03-47/2023-01/200288 and 451-03-47/2023-01/ 200045.

References:
[1] E. Rosa-Martínez, M. D. García-Martínez, A. M. Adalid-Martínez, L. Pereira-Dias, C. Casanova, E. Soler, M. Rosario Figas, M. Dolores
Raigon, M. Plazas, S. Soler, J. Prohens, Food Research International, 147 (2021) 110531.
[2] C. Agius, S. von Tucher, B. Poppenberger, W. Rozhon, MethodsX, 5 (2018). 537-550.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023
T1  - The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars
SP  - 87
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5976
ER  - 

@conference{
author = "Cvijanović, Vojin and Dramićanin, Aleksandra and Sarić, Beka and Jakanovski, Mihajlo V. and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Interest in production of protected crops has grown significantly over the past decade. Different cultivation systems (conventional, integral, and organic) affect the biological activity, which is ultimately reflected in the high quality of the fruits of different vegetable crops. In accordance with the requirements for health-safe products without residues of harmful substances in fruits, there is an increasing need for the production of tomatoes in integral and organic production systems. One of the most important features of these vegetables is their high quality and health safety, high nutritional and biological value. Agricultural production systems and growing practices are critical factors in determining the nutritional quality of tomato fruits [1]. In tomato, the sugar content is one of the important factors and one of the most significant parameters from the aspect of food quality [1]. Therefore, the aim of this work is to monitor fluctuations in sugars content, as parameters
that determine the nutritional value in tomato cultivars, induced by growing under integral and organic conditions. For this purpose, a set of sixteen samples of four types of tomatoes - beef, grapolo, mini and midi plum, and cherry - was analyzed. Each type of tomato included two varieties, grown in two agricultural systems - integral and organic. The sugars profile was obtained using High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAECPAD). The content of eleven sugar components was determined. Fructose and glucose were the major sugar compounds [2], while the sugar microcomponents were trehalose, arabinose, melibiose, sucrose, isomaltose, gentiobiose, raffinose, maltose, and panose. The results showed differences between samples produced in integral and organic growing systems, primarily in microsugar components. Higher content of trehalose and melibiose was found in samples obtained from organic production.

Fig.1. Principal Component Analysis (A)- The difference between integral (I) and organic (O) type of production; score plot (1a) – tomato samples: integral samples (I) 9-14, organic samples (O) 1-8, and loading plot (1b), and results of the Mann-Whitney U test (B) - sugar markers of type of production.

Acknowledgments: The authors would like to thank the Zeleni hit d.o.o. from Belgrade, Serbia, for field trials and support. This work has been supported by the Ministry of Science, Technological 
Development and Innovation of Republic of Serbia, Contract number: 451-03- 47/2023-01/200168, 451-03-47/2023-01/200288 and 451-03-47/2023-01/ 200045.

References:
[1] E. Rosa-Martínez, M. D. García-Martínez, A. M. Adalid-Martínez, L. Pereira-Dias, C. Casanova, E. Soler, M. Rosario Figas, M. Dolores
Raigon, M. Plazas, S. Soler, J. Prohens, Food Research International, 147 (2021) 110531.
[2] C. Agius, S. von Tucher, B. Poppenberger, W. Rozhon, MethodsX, 5 (2018). 537-550.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023",
title = "The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5976"
}

Cvijanović, V., Dramićanin, A., Sarić, B., Jakanovski, M. V., Momirović, N.,& Milojković-Opsenica, D.. (2023). The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023
Beograd : Srpsko hemijsko društvo., 87-87.
https://hdl.handle.net/21.15107/rcub_cherry_5976

Cvijanović V, Dramićanin A, Sarić B, Jakanovski MV, Momirović N, Milojković-Opsenica D. The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_cherry_5976 .

Cvijanović, Vojin, Dramićanin, Aleksandra, Sarić, Beka, Jakanovski, Mihajlo V., Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The influence of integral and organic growing systems on sugar content in selected tomato types and cultivars" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023 (2023):87-87,
https://hdl.handle.net/21.15107/rcub_cherry_5976 .

TY  - JOUR
AU  - Šegan, Sandra B.
AU  - Jevtić, Ivana I.
AU  - Tosti, Tomislav
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Kostić-Rajačić, Slađana
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5649
AB  - Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-
layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of the
piperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-
reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constant
of fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-
ophilicity, expressed as retention indices RM
0 , b, and C0, as well as PC1, was determined and correlated with in
silico values. Ionization constants were determined on the basis of the relationships between analyte’s retention
expressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, were
subjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to provide
basic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeation
was investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-
lecular weight. In general, results of the present research corroborate well with previously determined anti-
nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, another
set of important parameters should be taken into account when designing new derivatives of C-3 substituted
fentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLC
can be considered as a valuable asset in the ligand-based drug design
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography
VL  - 1211
SP  - 123481
DO  - 10.1016/j.jchromb.2022.123481
ER  - 

@article{
author = "Šegan, Sandra B. and Jevtić, Ivana I. and Tosti, Tomislav and Penjišević, Jelena and Šukalović, Vladimir and Kostić-Rajačić, Slađana and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-
layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of the
piperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-
reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constant
of fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-
ophilicity, expressed as retention indices RM
0 , b, and C0, as well as PC1, was determined and correlated with in
silico values. Ionization constants were determined on the basis of the relationships between analyte’s retention
expressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, were
subjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to provide
basic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeation
was investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-
lecular weight. In general, results of the present research corroborate well with previously determined anti-
nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, another
set of important parameters should be taken into account when designing new derivatives of C-3 substituted
fentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLC
can be considered as a valuable asset in the ligand-based drug design",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography",
volume = "1211",
pages = "123481",
doi = "10.1016/j.jchromb.2022.123481"
}

Šegan, S. B., Jevtić, I. I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B
Elsevier., 1211, 123481.
https://doi.org/10.1016/j.jchromb.2022.123481

Šegan SB, Jevtić II, Tosti T, Penjišević J, Šukalović V, Kostić-Rajačić S, Milojković-Opsenica D. Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B. 2022;1211:123481.
doi:10.1016/j.jchromb.2022.123481 .

Šegan, Sandra B., Jevtić, Ivana I., Tosti, Tomislav, Penjišević, Jelena, Šukalović, Vladimir, Kostić-Rajačić, Slađana, Milojković-Opsenica, Dušanka, "Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography" in Journal of Chromatography B, 1211 (2022):123481,
https://doi.org/10.1016/j.jchromb.2022.123481 . .

TY  - DATA
AU  - Šegan, Sandra B.
AU  - Jevtić, Ivana I.
AU  - Tosti, Tomislav
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Kostić-Rajačić, Slađana
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5650
AB  - Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of thepiperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constantof fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-ophilicity, expressed as retention indices RM0 , b, and C0, as well as PC1, was determined and correlated with insilico values. Ionization constants were determined on the basis of the relationships between analyte’s retentionexpressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, weresubjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to providebasic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeationwas investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-lecular weight. In general, results of the present research corroborate well with previously determined anti-nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, anotherset of important parameters should be taken into account when designing new derivatives of C-3 substitutedfentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLCcan be considered as a valuable asset in the ligand-based drug design
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481
VL  - 1211
SP  - 123481
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5650
ER  - 

@misc{
author = "Šegan, Sandra B. and Jevtić, Ivana I. and Tosti, Tomislav and Penjišević, Jelena and Šukalović, Vladimir and Kostić-Rajačić, Slađana and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of thepiperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constantof fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-ophilicity, expressed as retention indices RM0 , b, and C0, as well as PC1, was determined and correlated with insilico values. Ionization constants were determined on the basis of the relationships between analyte’s retentionexpressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, weresubjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to providebasic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeationwas investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-lecular weight. In general, results of the present research corroborate well with previously determined anti-nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, anotherset of important parameters should be taken into account when designing new derivatives of C-3 substitutedfentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLCcan be considered as a valuable asset in the ligand-based drug design",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481",
volume = "1211",
pages = "123481",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5650"
}

Šegan, S. B., Jevtić, I. I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481. in Journal of Chromatography B
Elsevier., 1211, 123481.
https://hdl.handle.net/21.15107/rcub_cherry_5650

Šegan SB, Jevtić II, Tosti T, Penjišević J, Šukalović V, Kostić-Rajačić S, Milojković-Opsenica D. Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481. in Journal of Chromatography B. 2022;1211:123481.
https://hdl.handle.net/21.15107/rcub_cherry_5650 .

Šegan, Sandra B., Jevtić, Ivana I., Tosti, Tomislav, Penjišević, Jelena, Šukalović, Vladimir, Kostić-Rajačić, Slađana, Milojković-Opsenica, Dušanka, "Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481" in Journal of Chromatography B, 1211 (2022):123481,
https://hdl.handle.net/21.15107/rcub_cherry_5650 .

TY  - JOUR
AU  - Brčić-Karačonji, Irena
AU  - Jurica, Karlo
AU  - Gašić, Uroš M.
AU  - Dramićanin, Aleksandra M.
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5014
AB  - The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean
shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial,
antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the
presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total
phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected
at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid
chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was
determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of
64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside
and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were
the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and
fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed
that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate
samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of
67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA
for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 µmol Trolox equivalents
(TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves.
Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to
A. unedo as a promising health-promoting plant and natural food preservative.
PB  - MDPI
T2  - Plants
T1  - Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
VL  - 11
IS  - 1
SP  - 25
DO  - 10.3390/plants11010025
ER  - 

@article{
author = "Brčić-Karačonji, Irena and Jurica, Karlo and Gašić, Uroš M. and Dramićanin, Aleksandra M. and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean
shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial,
antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the
presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total
phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected
at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid
chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was
determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of
64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside
and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were
the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and
fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed
that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate
samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of
67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA
for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 µmol Trolox equivalents
(TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves.
Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to
A. unedo as a promising health-promoting plant and natural food preservative.",
publisher = "MDPI",
journal = "Plants",
title = "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits",
volume = "11",
number = "1",
pages = "25",
doi = "10.3390/plants11010025"
}

Brčić-Karačonji, I., Jurica, K., Gašić, U. M., Dramićanin, A. M., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2022). Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits. in Plants
MDPI., 11(1), 25.
https://doi.org/10.3390/plants11010025

Brčić-Karačonji I, Jurica K, Gašić UM, Dramićanin AM, Tešić ŽL, Milojković-Opsenica D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits. in Plants. 2022;11(1):25.
doi:10.3390/plants11010025 .

Brčić-Karačonji, Irena, Jurica, Karlo, Gašić, Uroš M., Dramićanin, Aleksandra M., Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits" in Plants, 11, no. 1 (2022):25,
https://doi.org/10.3390/plants11010025 . .

TY  - JOUR
AU  - Ljoljić Bilić, Vanja
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Rimac, Hrvoje
AU  - Vuković Rodriguez, Jadranka
AU  - Vlainić, Josipa
AU  - Brlek‐Gorski, Diana
AU  - Kosalec, Ivan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5197
AB  - Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation
PB  - MDPI
T2  - Аntibiotics
T1  - Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus
VL  - 11
SP  - 492
DO  - 10.3390/antibiotics11040492
ER  - 

@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek‐Gorski, Diana and Kosalec, Ivan",
year = "2022",
abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation",
publisher = "MDPI",
journal = "Аntibiotics",
title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus",
volume = "11",
pages = "492",
doi = "10.3390/antibiotics11040492"
}

Ljoljić Bilić, V., Gašić, U. M., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek‐Gorski, D.,& Kosalec, I.. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics
MDPI., 11, 492.
https://doi.org/10.3390/antibiotics11040492

Ljoljić Bilić V, Gašić UM, Milojković-Opsenica D, Rimac H, Vuković Rodriguez J, Vlainić J, Brlek‐Gorski D, Kosalec I. Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics. 2022;11:492.
doi:10.3390/antibiotics11040492 .

Ljoljić Bilić, Vanja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Rimac, Hrvoje, Vuković Rodriguez, Jadranka, Vlainić, Josipa, Brlek‐Gorski, Diana, Kosalec, Ivan, "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus" in Аntibiotics, 11 (2022):492,
https://doi.org/10.3390/antibiotics11040492 . .