TY  - JOUR
AU  - Bogdanović, Jelena
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
PY  - 2006
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/237
AB  - Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount.
AB  - Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.
T2  - Archives of biological sciences
T1  - Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]
T1  - Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]
VL  - 58
IS  - 4
SP  - 211
EP  - 214
DO  - 10.2298/ABS0604211B
ER  - 

@article{
author = "Bogdanović, Jelena and Prodanović, Radivoje and Milosavić, Nenad and Prodanović, Olivera and Radotić, Ksenija",
year = "2006",
abstract = "Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panč.) Purkyně, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount., Proučavana je aktivnost i izoenzimski sastav superoksid dismutaze u apoplastu i ekstraktu celih četina omorike [Picea omorika (Panč.) Purkyně]. Ukupna aktivnost superoksid dismutaze u solubilnoj frakciji ekstrakta četina je bila značajno veća od aktivnosti superoksid dismutaze u apoplastu. U ekstraktu celih četina nađene su nekoliko kiselih i dve sporo-migrirajuće bazne izoforme. Vanćelijska superoksid dismutaza sadrži samo jednu ekstremno kiselu izoformu. Koristeći specifične inhibitore, Cu/Zn- i Mn- superoksid dismutazne forme su identifikovane u ekstraktu celih četina, dok je u apoplastu nađena samo Mn- superoksid dismutaza. Feizoforma nije prisutna u merljivoj koncentraciji.",
journal = "Archives of biological sciences",
title = "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně], Različite izoforme superoksid dismutaze u apoplastu i ekstraktu četina omorike [Picea omorika (Panč.) Purkyně]",
volume = "58",
number = "4",
pages = "211-214",
doi = "10.2298/ABS0604211B"
}

Bogdanović, J., Prodanović, R., Milosavić, N., Prodanović, O.,& Radotić, K.. (2006). Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of biological sciences, 58(4), 211-214.
https://doi.org/10.2298/ABS0604211B

Bogdanović J, Prodanović R, Milosavić N, Prodanović O, Radotić K. Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]. in Archives of biological sciences. 2006;58(4):211-214.
doi:10.2298/ABS0604211B .

Bogdanović, Jelena, Prodanović, Radivoje, Milosavić, Nenad, Prodanović, Olivera, Radotić, Ksenija, "Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panč.) Purkyně]" in Archives of biological sciences, 58, no. 4 (2006):211-214,
https://doi.org/10.2298/ABS0604211B . .

TY  - JOUR
AU  - Bogdanović, Jelena
AU  - Milosavljević, Nenad
AU  - Vujčić, Zoran
AU  - Radotić, Ksenija
PY  - 2004
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/221
AB  - We studied the effect of nonionic detergent Tween 80 in extraction buffer on the activity and isoenzyme pattern of peroxidase from omorika needles. The activity of soluble peroxidases was studied using UV-VIS spectrophotometry and isoelectrofocusing. Soluble fraction obtained using extraction buffer with detergent had a higher activity and more isoenzymes in IEF pattern in comparison with the corresponding extract not including detergent in extraction medium.
AB  - Ispitivan je uticaj nejonskog detergenta Tween 80 u ekstrakcionom puferu na aktivnost i izoenzimski sastav peroksidaza u četinama omorike (Picea omorika (Pančić) Purkinye). Aktivnost unutarćelijske i vanćelijske slobodne peroksidaze ispitivana je korišćenjem UV-VIS spektrofotometrije i izoelektrofokusiranja. Ekstrakti dobijeni korišćenjem ekstrakcionog pufera sa detergentom Tween 80 imaju veću peroksidaznu aktivnost i veći broj izoenzima dobijenih izoelektrofokusiranjem u poređenju sa ekstraktima čiji ekstrakcioni pufer nije sadržao nejonski detergent.
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses
T1  - Priprema ekstrakta omorike za analizu aktivnosti peroksidaze i izoenzima
VL  - 40
IS  - 1-3
SP  - 159
EP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_cherry_221
ER  - 

@article{
author = "Bogdanović, Jelena and Milosavljević, Nenad and Vujčić, Zoran and Radotić, Ksenija",
year = "2004",
abstract = "We studied the effect of nonionic detergent Tween 80 in extraction buffer on the activity and isoenzyme pattern of peroxidase from omorika needles. The activity of soluble peroxidases was studied using UV-VIS spectrophotometry and isoelectrofocusing. Soluble fraction obtained using extraction buffer with detergent had a higher activity and more isoenzymes in IEF pattern in comparison with the corresponding extract not including detergent in extraction medium., Ispitivan je uticaj nejonskog detergenta Tween 80 u ekstrakcionom puferu na aktivnost i izoenzimski sastav peroksidaza u četinama omorike (Picea omorika (Pančić) Purkinye). Aktivnost unutarćelijske i vanćelijske slobodne peroksidaze ispitivana je korišćenjem UV-VIS spektrofotometrije i izoelektrofokusiranja. Ekstrakti dobijeni korišćenjem ekstrakcionog pufera sa detergentom Tween 80 imaju veću peroksidaznu aktivnost i veći broj izoenzima dobijenih izoelektrofokusiranjem u poređenju sa ekstraktima čiji ekstrakcioni pufer nije sadržao nejonski detergent.",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses, Priprema ekstrakta omorike za analizu aktivnosti peroksidaze i izoenzima",
volume = "40",
number = "1-3",
pages = "159-164",
url = "https://hdl.handle.net/21.15107/rcub_cherry_221"
}

Bogdanović, J., Milosavljević, N., Vujčić, Z.,& Radotić, K.. (2004). Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 40(1-3), 159-164.
https://hdl.handle.net/21.15107/rcub_cherry_221

Bogdanović J, Milosavljević N, Vujčić Z, Radotić K. Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2004;40(1-3):159-164.
https://hdl.handle.net/21.15107/rcub_cherry_221 .

Bogdanović, Jelena, Milosavljević, Nenad, Vujčić, Zoran, Radotić, Ksenija, "Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 40, no. 1-3 (2004):159-164,
https://hdl.handle.net/21.15107/rcub_cherry_221 .