TY  - JOUR
AU  - Gavrilović, Milan
AU  - Tešević, Vele
AU  - Đorđević, Ivana S.
AU  - Rajčević, Nemanja
AU  - Bakhia, A.
AU  - Jacas, N.G.
AU  - Susanna, A.
AU  - Marin, Petar D.
AU  - Janaćković, Peđa T.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2808
AB  - We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine.
T2  - Archives of Biological Sciences
T2  - Archives of Biological Sciences
T1  - Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia
VL  - 70
IS  - 4
SP  - 613
EP  - 620
DO  - 10.2298/ABS180309022G
ER  - 

@article{
author = "Gavrilović, Milan and Tešević, Vele and Đorđević, Ivana S. and Rajčević, Nemanja and Bakhia, A. and Jacas, N.G. and Susanna, A. and Marin, Petar D. and Janaćković, Peđa T.",
year = "2018",
abstract = "We examined for the first time the leaf micromorphology, phytochemistry and biological activity of the rare and stenoendemic Amphoricarpos elegans Albov (Compositae) from Georgia. Scanning electron microscopy (SEM) revealed the presence of glandular trichomes on the leaves, which appeared as glandular dots that are considered the main sites of biosynthesis and accumulation of sesquiterpene lactones. Using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identify and characterized 3-deoxyamphoricarpolide, a known sesquiterpene lactone for the genus Amphoricarpos Vis. Regarding chemotaxonomic significance, 3-deoxyamphoricarpolide represents a link between Balkan and Caucasian species of the genus. The antioxidative capacity of different leaf extracts, obtained using a Soxhlet extractor, was evaluated by two radical scavenging assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and the 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and ferric ion reducing antioxidant power (FRAP). The total phenolic and flavonoid contents were also determined. The highest antioxidative activity and the highest phenolic and flavonoid contents were detected in the methanol fraction, as a result of the contribution of not only phenols, but probably also lactones. The considerable antioxidative potential indicates possible applications in pharmacy and medicine.",
journal = "Archives of Biological Sciences, Archives of Biological Sciences",
title = "Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia",
volume = "70",
number = "4",
pages = "613-620",
doi = "10.2298/ABS180309022G"
}

Gavrilović, M., Tešević, V., Đorđević, I. S., Rajčević, N., Bakhia, A., Jacas, N.G., Susanna, A., Marin, P. D.,& Janaćković, P. T.. (2018). Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia. in Archives of Biological Sciences, 70(4), 613-620.
https://doi.org/10.2298/ABS180309022G

Gavrilović M, Tešević V, Đorđević IS, Rajčević N, Bakhia A, Jacas N, Susanna A, Marin PD, Janaćković PT. Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia. in Archives of Biological Sciences. 2018;70(4):613-620.
doi:10.2298/ABS180309022G .

Gavrilović, Milan, Tešević, Vele, Đorđević, Ivana S., Rajčević, Nemanja, Bakhia, A., Jacas, N.G., Susanna, A., Marin, Petar D., Janaćković, Peđa T., "Leaf micromorphology, antioxidative activity and a new record of 3-deoxyamphoricarpolide of relict and limestone endemic Amphoricarpos elegans Albov (Compositae) from Georgia" in Archives of Biological Sciences, 70, no. 4 (2018):613-620,
https://doi.org/10.2298/ABS180309022G . .