TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag V.
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6270
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
SP  - 1148309
DO  - 10.3389/fevo.2023.1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag V. and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
pages = "1148309",
doi = "10.3389/fevo.2023.1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L. V., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić LV, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag V., Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Bukvicki, Danka
AU  - Giweli, Abdulhmid
AU  - Stojković, Dejan
AU  - Vujisić, Ljubodrag V.
AU  - Tešević, Vele
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Marin, Petar D.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2140
AB  - The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4 degrees C with 25 mu L of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).
PB  - Elsevier Science Inc, New York
T2  - Journal of Dairy Science
T1  - Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative
VL  - 101
IS  - 5
SP  - 3859
EP  - 3865
DO  - 10.3168/jds.2017-13714
ER  - 

@article{
author = "Bukvicki, Danka and Giweli, Abdulhmid and Stojković, Dejan and Vujisić, Ljubodrag V. and Tešević, Vele and Nikolić, Miloš and Soković, Marina and Marin, Petar D.",
year = "2018",
abstract = "The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4 degrees C with 25 mu L of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Dairy Science",
title = "Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative",
volume = "101",
number = "5",
pages = "3859-3865",
doi = "10.3168/jds.2017-13714"
}

Bukvicki, D., Giweli, A., Stojković, D., Vujisić, L. V., Tešević, V., Nikolić, M., Soković, M.,& Marin, P. D.. (2018). Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative. in Journal of Dairy Science
Elsevier Science Inc, New York., 101(5), 3859-3865.
https://doi.org/10.3168/jds.2017-13714

Bukvicki D, Giweli A, Stojković D, Vujisić LV, Tešević V, Nikolić M, Soković M, Marin PD. Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative. in Journal of Dairy Science. 2018;101(5):3859-3865.
doi:10.3168/jds.2017-13714 .

Bukvicki, Danka, Giweli, Abdulhmid, Stojković, Dejan, Vujisić, Ljubodrag V., Tešević, Vele, Nikolić, Miloš, Soković, Marina, Marin, Petar D., "Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative" in Journal of Dairy Science, 101, no. 5 (2018):3859-3865,
https://doi.org/10.3168/jds.2017-13714 . .