TY  - DATA
AU  - Spasić, Jelena
AU  - Mandić, Mina
AU  - Đokić, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3263
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Supplementary data for the article: Spasic, J.; Mandic, M.; Djokic, L.; Nikodinovic-Runic, J. Streptomyces Spp. in the Biocatalysis Toolbox. Appl. Microbiol. Biotechnol. 2018, 102 (8), 3513–3536. https://doi.org/10.1007/s00253-018-8884-x
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3263
ER  - 

@misc{
author = "Spasić, Jelena and Mandić, Mina and Đokić, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Supplementary data for the article: Spasic, J.; Mandic, M.; Djokic, L.; Nikodinovic-Runic, J. Streptomyces Spp. in the Biocatalysis Toolbox. Appl. Microbiol. Biotechnol. 2018, 102 (8), 3513–3536. https://doi.org/10.1007/s00253-018-8884-x",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3263"
}

Spasić, J., Mandić, M., Đokić, L.,& Nikodinović-Runić, J.. (2018). Supplementary data for the article: Spasic, J.; Mandic, M.; Djokic, L.; Nikodinovic-Runic, J. Streptomyces Spp. in the Biocatalysis Toolbox. Appl. Microbiol. Biotechnol. 2018, 102 (8), 3513–3536. https://doi.org/10.1007/s00253-018-8884-x. in Applied Microbiology and Biotechnology
Springer, New York..
https://hdl.handle.net/21.15107/rcub_cherry_3263

Spasić J, Mandić M, Đokić L, Nikodinović-Runić J. Supplementary data for the article: Spasic, J.; Mandic, M.; Djokic, L.; Nikodinovic-Runic, J. Streptomyces Spp. in the Biocatalysis Toolbox. Appl. Microbiol. Biotechnol. 2018, 102 (8), 3513–3536. https://doi.org/10.1007/s00253-018-8884-x. in Applied Microbiology and Biotechnology. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3263 .

Spasić, Jelena, Mandić, Mina, Đokić, Lidija, Nikodinović-Runić, Jasmina, "Supplementary data for the article: Spasic, J.; Mandic, M.; Djokic, L.; Nikodinovic-Runic, J. Streptomyces Spp. in the Biocatalysis Toolbox. Appl. Microbiol. Biotechnol. 2018, 102 (8), 3513–3536. https://doi.org/10.1007/s00253-018-8884-x" in Applied Microbiology and Biotechnology (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3263 .

TY  - JOUR
AU  - Spasić, Jelena
AU  - Mandić, Mina
AU  - Đokić, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2115
AB  - About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Streptomyces spp. in the biocatalysis toolbox
VL  - 102
IS  - 8
SP  - 3513
EP  - 3536
DO  - 10.1007/s00253-018-8884-x
ER  - 

@article{
author = "Spasić, Jelena and Mandić, Mina and Đokić, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
abstract = "About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Streptomyces spp. in the biocatalysis toolbox",
volume = "102",
number = "8",
pages = "3513-3536",
doi = "10.1007/s00253-018-8884-x"
}

Spasić, J., Mandić, M., Đokić, L.,& Nikodinović-Runić, J.. (2018). Streptomyces spp. in the biocatalysis toolbox. in Applied Microbiology and Biotechnology
Springer, New York., 102(8), 3513-3536.
https://doi.org/10.1007/s00253-018-8884-x

Spasić J, Mandić M, Đokić L, Nikodinović-Runić J. Streptomyces spp. in the biocatalysis toolbox. in Applied Microbiology and Biotechnology. 2018;102(8):3513-3536.
doi:10.1007/s00253-018-8884-x .

Spasić, Jelena, Mandić, Mina, Đokić, Lidija, Nikodinović-Runić, Jasmina, "Streptomyces spp. in the biocatalysis toolbox" in Applied Microbiology and Biotechnology, 102, no. 8 (2018):3513-3536,
https://doi.org/10.1007/s00253-018-8884-x . .

TY  - JOUR
AU  - Lazić, Jelena O.
AU  - Spasić, Jelena
AU  - Francuski, Đorđe
AU  - Tokić-Vujošević, Zorana
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, Veselin
AU  - Đokić, Lidija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2324
AB  - Michael addition of aldehydes to nitro-olefins is an effective method to obtain useful chiral gamma-nitroaldehydes. gamma-Nitroaldehydes are precursors for chiral gamma-aminobutyric acid analogues, which have numerous pharmacological activities and are used for the treatment of neurological disorders. A whole-cell system based on recombinantly expressed 4-oxalocrotonate tautomerase (4-OT) was developed and shown to be an effective biocatalyst for the Michael addition of branched aldehydes to beta-nitrostyrenes. The aim of this study was to investigate the influence of the substitution of the N-terminal proline with lysine and arginine, both containing a reactive epsilon-amino group, on the Michael addition catalyzed by 4-OT. First, the effects of these mutations were examined by in silico analysis, followed by the generation of three terminal lysine mutants. The generated mutants, 4-OT_K, 4-OT_PK and 4-OT_KK were tested for their ability to utilise beta-nitrostyrene (1), (E)-1-nitro-2-(2-thienyl)ethene (2) and trans-p-chloro-beta-nitrostyrene (3) as Michael acceptors with isobutanal (2-methylpropanal) as the donor. For comparison, the lithium salt of lysine was used in the same organocatalytic reactions. In general, the introduction of lysine had a negative effect on Michael additions based on overall product yields. However, additional lysine residues at the N-terminus of the protein resulted in structural changes that enhanced the activity towards 2 and 3. Therefore, the N-terminal proline is important for 4-OT-catalysed Michael-additions, but it is not essential.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Importance of N-terminal proline for the promiscuous activity of 4-oxalocrotonate tautomerase (4-OT)
VL  - 81
IS  - 8
SP  - 871
EP  - 881
DO  - 10.2298/JSC160222053L
ER  - 

@article{
author = "Lazić, Jelena O. and Spasić, Jelena and Francuski, Đorđe and Tokić-Vujošević, Zorana and Nikodinović-Runić, Jasmina and Maslak, Veselin and Đokić, Lidija",
year = "2016",
abstract = "Michael addition of aldehydes to nitro-olefins is an effective method to obtain useful chiral gamma-nitroaldehydes. gamma-Nitroaldehydes are precursors for chiral gamma-aminobutyric acid analogues, which have numerous pharmacological activities and are used for the treatment of neurological disorders. A whole-cell system based on recombinantly expressed 4-oxalocrotonate tautomerase (4-OT) was developed and shown to be an effective biocatalyst for the Michael addition of branched aldehydes to beta-nitrostyrenes. The aim of this study was to investigate the influence of the substitution of the N-terminal proline with lysine and arginine, both containing a reactive epsilon-amino group, on the Michael addition catalyzed by 4-OT. First, the effects of these mutations were examined by in silico analysis, followed by the generation of three terminal lysine mutants. The generated mutants, 4-OT_K, 4-OT_PK and 4-OT_KK were tested for their ability to utilise beta-nitrostyrene (1), (E)-1-nitro-2-(2-thienyl)ethene (2) and trans-p-chloro-beta-nitrostyrene (3) as Michael acceptors with isobutanal (2-methylpropanal) as the donor. For comparison, the lithium salt of lysine was used in the same organocatalytic reactions. In general, the introduction of lysine had a negative effect on Michael additions based on overall product yields. However, additional lysine residues at the N-terminus of the protein resulted in structural changes that enhanced the activity towards 2 and 3. Therefore, the N-terminal proline is important for 4-OT-catalysed Michael-additions, but it is not essential.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Importance of N-terminal proline for the promiscuous activity of 4-oxalocrotonate tautomerase (4-OT)",
volume = "81",
number = "8",
pages = "871-881",
doi = "10.2298/JSC160222053L"
}

Lazić, J. O., Spasić, J., Francuski, Đ., Tokić-Vujošević, Z., Nikodinović-Runić, J., Maslak, V.,& Đokić, L.. (2016). Importance of N-terminal proline for the promiscuous activity of 4-oxalocrotonate tautomerase (4-OT). in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 81(8), 871-881.
https://doi.org/10.2298/JSC160222053L

Lazić JO, Spasić J, Francuski Đ, Tokić-Vujošević Z, Nikodinović-Runić J, Maslak V, Đokić L. Importance of N-terminal proline for the promiscuous activity of 4-oxalocrotonate tautomerase (4-OT). in Journal of the Serbian Chemical Society. 2016;81(8):871-881.
doi:10.2298/JSC160222053L .

Lazić, Jelena O., Spasić, Jelena, Francuski, Đorđe, Tokić-Vujošević, Zorana, Nikodinović-Runić, Jasmina, Maslak, Veselin, Đokić, Lidija, "Importance of N-terminal proline for the promiscuous activity of 4-oxalocrotonate tautomerase (4-OT)" in Journal of the Serbian Chemical Society, 81, no. 8 (2016):871-881,
https://doi.org/10.2298/JSC160222053L . .

TY  - DATA
AU  - Đokić, Lidija
AU  - Spasić, Jelena
AU  - Jeremić, Sanja
AU  - Vasiljević, Branka
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
AU  - Nikodinović-Runić, Jasmina
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3377
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3377
ER  - 

@misc{
author = "Đokić, Lidija and Spasić, Jelena and Jeremić, Sanja and Vasiljević, Branka and Prodanović, Olivera and Prodanović, Radivoje and Nikodinović-Runić, Jasmina",
year = "2015",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3377"
}

Đokić, L., Spasić, J., Jeremić, S., Vasiljević, B., Prodanović, O., Prodanović, R.,& Nikodinović-Runić, J.. (2015). Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8. in Bioprocess and Biosystems Engineering
Springer, New York..
https://hdl.handle.net/21.15107/rcub_cherry_3377

Đokić L, Spasić J, Jeremić S, Vasiljević B, Prodanović O, Prodanović R, Nikodinović-Runić J. Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8. in Bioprocess and Biosystems Engineering. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3377 .

Đokić, Lidija, Spasić, Jelena, Jeremić, Sanja, Vasiljević, Branka, Prodanović, Olivera, Prodanović, Radivoje, Nikodinović-Runić, Jasmina, "Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8" in Bioprocess and Biosystems Engineering (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3377 .

TY  - JOUR
AU  - Đokić, Lidija
AU  - Spasić, Jelena
AU  - Jeremić, Sanja
AU  - Vasiljević, Branka
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
AU  - Nikodinović-Runić, Jasmina
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1990
AB  - The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to beta-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from beta-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene
VL  - 38
IS  - 12
SP  - 2389
EP  - 2395
DO  - 10.1007/s00449-015-1474-8
ER  - 

@article{
author = "Đokić, Lidija and Spasić, Jelena and Jeremić, Sanja and Vasiljević, Branka and Prodanović, Olivera and Prodanović, Radivoje and Nikodinović-Runić, Jasmina",
year = "2015",
abstract = "The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to beta-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from beta-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene",
volume = "38",
number = "12",
pages = "2389-2395",
doi = "10.1007/s00449-015-1474-8"
}

Đokić, L., Spasić, J., Jeremić, S., Vasiljević, B., Prodanović, O., Prodanović, R.,& Nikodinović-Runić, J.. (2015). Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene. in Bioprocess and Biosystems Engineering
Springer, New York., 38(12), 2389-2395.
https://doi.org/10.1007/s00449-015-1474-8

Đokić L, Spasić J, Jeremić S, Vasiljević B, Prodanović O, Prodanović R, Nikodinović-Runić J. Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene. in Bioprocess and Biosystems Engineering. 2015;38(12):2389-2395.
doi:10.1007/s00449-015-1474-8 .

Đokić, Lidija, Spasić, Jelena, Jeremić, Sanja, Vasiljević, Branka, Prodanović, Olivera, Prodanović, Radivoje, Nikodinović-Runić, Jasmina, "Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene" in Bioprocess and Biosystems Engineering, 38, no. 12 (2015):2389-2395,
https://doi.org/10.1007/s00449-015-1474-8 . .

TY  - JOUR
AU  - Đinović-Stojanović, Jasna
AU  - Spasić, Jelena
AU  - Popović, Aleksandar R.
AU  - Vranić, Danijela
AU  - Nikčević, Miroslav
AU  - Hegediš, Aleksandar
AU  - Nikolić, Dragica
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/183
AB  - The distribution and contents of organochlorine pesticides (OCPs) and congeners of polychlorinated biphenyls (PCBs) were analyzed in Bream (Blicca bjoerkna) and Barbel (Barbus barbus) from the Danube River, near Batajnica. The analysed compounds were 16 OCPs (α-Hexachlorocyclohexane (α-HCH), β-Hexachlorocyclohexane (β-HCH), Hexachlorobenzene (HCB), γ-Hexachlorocyclohexane (γ-HCH), δ-Hexachlorocyclohexane (δ-HCH), Heptachlor, Aldrin, cis-Heptachloroepoxide (cis-HCE), trans-Heptachloroepoxide (trans-HCE), γ-Chlordane, p,p'-Dichlorodiphenyldichloroethylene (p,p'-DDE), α-Chlordane, Dieldrin, Endrin, p,p'-Dichlorodiphenyldichloroethane (p,p'-DDD), p,p'-Dichlorodiphenyltri-chloroethane (p,p'-DDT)) and 7 PCB congeners (IUPAC numbers 28, 52, 101, 138, 153, 180, 118). Determination and quantification of OCPs and PCBs were performed by a GC-ECD method. The proximate composition of fish fillets was determined by applying standard SRPS ISO methods. The statistically significant difference (p  lt  0.05) was found between the OCPs content in Bream and Barbel, as well as between PCBs content in the analyzed fish filets. Among all analyzed pesticides, both fish contained the highest amounts of ΣDDT (expressed as sum of p,p'-DDT + p,p'-DDE + p,p'-DDD), (13.8 ng/g fish - Bream, 2.6 ng/g fish - Barbel). The sum of PCB congeners in Bream filets (40.8 ng/g fish) was significantly higher than in Barbel filets (7.2 ng/g fish). Results of Student's t-test (p = 0.05) showed the differences in the content of lipids, moisture, ash and calculated energy value between the analyzed fish filets. Four times higher fat content in Bream (4.25%) than in Barbel (1.07%) is one of the main reasons for higher content of OCPs and PCBs in Bream compared to Barbel, which were taken from the same location (The Danube River, Batajnica).
AB  - U ovom radu prikazana je distribucija i sadržaj organohlornih pesticida (organochlorine pesticides, OCP) i polihlorovanih bifenila (polychlorinated biphenyls, PCB) u ribama krupatici (Blicca bjoerkna) i mreni (Barbus barbus) iz Dunava u blizini Batajnice. Ispitano je 16 organohlornih pesticida i 7 kongenera polihlorovanih bifenila. Anlizirana su sledeća jedinjenja: α-heksahlorocikloheksan (α-HCH), β-heksahlorocikloheksan (β-HCH), heksahlorobenzen (HCB), γ-heksahlorocikloheksan (γ-HCH), δ-heksahlorocikloheksan (δ-HCH), heptahlor, aldrin, cis-heptahloroepoksid (cis-HCE), trans-heptahloroepoksid (trans-HCE), γ-hlordan, α-hlordan, p,p'-dihlorodifenildihloroetilen (p,p'-DDE), dieldrin, endrin, p,p'-dihlorodifenildihloroetan (p,p'-DDD) i p,p'- dihlorodifeniltrihloroetan (p,p'-DDT) i PCB kongeneri označeni IUPAC brojevima 28, 52, 101, 138, 153, 180 i 118. Kvalitativna i kvanitativna ispitivanja ovih jedinjenja rađena su GC-ECD metodom. Sadržaj proteina, lipida, vlage i pepela u filetima riba određen je korišćenjem standardnih SRPS ISO metoda. Utvrđena je statistički značajna razlika (p  lt  0,05) između sadržaja organohlornih pesticida u krupatici i mreni, kao i između sadržaja polihlorovanih bifenila u ispitanim ribama. Najveći sadržaj među ispitivanim organohlornim pesticidima, u obe vrste ribe utvrđen je za ΣDDT (pp'-DDT + pp'-DDE + pp'-DDD), (13,8 ng/g ribe - krupatica, 2,6 ng/g ribe - mrena). Ukupan sadržaj PCB jedinjenja u filetima krupatice (40,8 ng/g ribe) bio je značajno veći nego u filetima mrene (7,2 ng/g ribe). Na osnovu rezultata Studentovog t-testa (p = 0,05) utvrđeno je da postoji statistički značajna razlika u sadržaju masti, vlage, pepela i izračunate energetske vrednosti između fileta ispitanih riba. Četiri puta veći sadržaj masti u filetima krupatice (4,25%) u odnosu na filete mrene (1,07%) jedan je od glavih razloga većeg sadržaja organohlornih pesticida i polihlorovanih bifenila u krupatici nego u mreni, koje su uzete sa istog lokaliteta (Dunav, Batajnica).
T2  - Tehnologija mesa
T1  - Distribution of organochlorine pesticides and polychlorinated biphenyls in two spices of fish from Danube
T1  - Distribucija organohlornih pesticida i polihlorovanih bifenila u dve vrste riba iz Dunava
VL  - 54
IS  - 1
SP  - 69
EP  - 78
DO  - 10.5937/tehmesa1301069D
ER  - 

@article{
author = "Đinović-Stojanović, Jasna and Spasić, Jelena and Popović, Aleksandar R. and Vranić, Danijela and Nikčević, Miroslav and Hegediš, Aleksandar and Nikolić, Dragica",
year = "2013",
abstract = "The distribution and contents of organochlorine pesticides (OCPs) and congeners of polychlorinated biphenyls (PCBs) were analyzed in Bream (Blicca bjoerkna) and Barbel (Barbus barbus) from the Danube River, near Batajnica. The analysed compounds were 16 OCPs (α-Hexachlorocyclohexane (α-HCH), β-Hexachlorocyclohexane (β-HCH), Hexachlorobenzene (HCB), γ-Hexachlorocyclohexane (γ-HCH), δ-Hexachlorocyclohexane (δ-HCH), Heptachlor, Aldrin, cis-Heptachloroepoxide (cis-HCE), trans-Heptachloroepoxide (trans-HCE), γ-Chlordane, p,p'-Dichlorodiphenyldichloroethylene (p,p'-DDE), α-Chlordane, Dieldrin, Endrin, p,p'-Dichlorodiphenyldichloroethane (p,p'-DDD), p,p'-Dichlorodiphenyltri-chloroethane (p,p'-DDT)) and 7 PCB congeners (IUPAC numbers 28, 52, 101, 138, 153, 180, 118). Determination and quantification of OCPs and PCBs were performed by a GC-ECD method. The proximate composition of fish fillets was determined by applying standard SRPS ISO methods. The statistically significant difference (p  lt  0.05) was found between the OCPs content in Bream and Barbel, as well as between PCBs content in the analyzed fish filets. Among all analyzed pesticides, both fish contained the highest amounts of ΣDDT (expressed as sum of p,p'-DDT + p,p'-DDE + p,p'-DDD), (13.8 ng/g fish - Bream, 2.6 ng/g fish - Barbel). The sum of PCB congeners in Bream filets (40.8 ng/g fish) was significantly higher than in Barbel filets (7.2 ng/g fish). Results of Student's t-test (p = 0.05) showed the differences in the content of lipids, moisture, ash and calculated energy value between the analyzed fish filets. Four times higher fat content in Bream (4.25%) than in Barbel (1.07%) is one of the main reasons for higher content of OCPs and PCBs in Bream compared to Barbel, which were taken from the same location (The Danube River, Batajnica)., U ovom radu prikazana je distribucija i sadržaj organohlornih pesticida (organochlorine pesticides, OCP) i polihlorovanih bifenila (polychlorinated biphenyls, PCB) u ribama krupatici (Blicca bjoerkna) i mreni (Barbus barbus) iz Dunava u blizini Batajnice. Ispitano je 16 organohlornih pesticida i 7 kongenera polihlorovanih bifenila. Anlizirana su sledeća jedinjenja: α-heksahlorocikloheksan (α-HCH), β-heksahlorocikloheksan (β-HCH), heksahlorobenzen (HCB), γ-heksahlorocikloheksan (γ-HCH), δ-heksahlorocikloheksan (δ-HCH), heptahlor, aldrin, cis-heptahloroepoksid (cis-HCE), trans-heptahloroepoksid (trans-HCE), γ-hlordan, α-hlordan, p,p'-dihlorodifenildihloroetilen (p,p'-DDE), dieldrin, endrin, p,p'-dihlorodifenildihloroetan (p,p'-DDD) i p,p'- dihlorodifeniltrihloroetan (p,p'-DDT) i PCB kongeneri označeni IUPAC brojevima 28, 52, 101, 138, 153, 180 i 118. Kvalitativna i kvanitativna ispitivanja ovih jedinjenja rađena su GC-ECD metodom. Sadržaj proteina, lipida, vlage i pepela u filetima riba određen je korišćenjem standardnih SRPS ISO metoda. Utvrđena je statistički značajna razlika (p  lt  0,05) između sadržaja organohlornih pesticida u krupatici i mreni, kao i između sadržaja polihlorovanih bifenila u ispitanim ribama. Najveći sadržaj među ispitivanim organohlornim pesticidima, u obe vrste ribe utvrđen je za ΣDDT (pp'-DDT + pp'-DDE + pp'-DDD), (13,8 ng/g ribe - krupatica, 2,6 ng/g ribe - mrena). Ukupan sadržaj PCB jedinjenja u filetima krupatice (40,8 ng/g ribe) bio je značajno veći nego u filetima mrene (7,2 ng/g ribe). Na osnovu rezultata Studentovog t-testa (p = 0,05) utvrđeno je da postoji statistički značajna razlika u sadržaju masti, vlage, pepela i izračunate energetske vrednosti između fileta ispitanih riba. Četiri puta veći sadržaj masti u filetima krupatice (4,25%) u odnosu na filete mrene (1,07%) jedan je od glavih razloga većeg sadržaja organohlornih pesticida i polihlorovanih bifenila u krupatici nego u mreni, koje su uzete sa istog lokaliteta (Dunav, Batajnica).",
journal = "Tehnologija mesa",
title = "Distribution of organochlorine pesticides and polychlorinated biphenyls in two spices of fish from Danube, Distribucija organohlornih pesticida i polihlorovanih bifenila u dve vrste riba iz Dunava",
volume = "54",
number = "1",
pages = "69-78",
doi = "10.5937/tehmesa1301069D"
}

Đinović-Stojanović, J., Spasić, J., Popović, A. R., Vranić, D., Nikčević, M., Hegediš, A.,& Nikolić, D.. (2013). Distribution of organochlorine pesticides and polychlorinated biphenyls in two spices of fish from Danube. in Tehnologija mesa, 54(1), 69-78.
https://doi.org/10.5937/tehmesa1301069D

Đinović-Stojanović J, Spasić J, Popović AR, Vranić D, Nikčević M, Hegediš A, Nikolić D. Distribution of organochlorine pesticides and polychlorinated biphenyls in two spices of fish from Danube. in Tehnologija mesa. 2013;54(1):69-78.
doi:10.5937/tehmesa1301069D .

Đinović-Stojanović, Jasna, Spasić, Jelena, Popović, Aleksandar R., Vranić, Danijela, Nikčević, Miroslav, Hegediš, Aleksandar, Nikolić, Dragica, "Distribution of organochlorine pesticides and polychlorinated biphenyls in two spices of fish from Danube" in Tehnologija mesa, 54, no. 1 (2013):69-78,
https://doi.org/10.5937/tehmesa1301069D . .