TY  - JOUR
AU  - Vlajkovic, Jelena
AU  - Andrić, Filip
AU  - Ristivojević, Petar
AU  - Radoičić, Aleksandra
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1374
AB  - A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance liquid chromatography (RP-HPLC) method. In the case of TLC method horizontal developing technique was employed using octadecyl-modified silica as a stationary phase and 0.5mol dm(-3) ammonium sulfate in 30% of ethanol-water solution as a mobile phase. HPLC analysis was carried out using XDB-C18 column and gradient of mixture of aqueous ammonium acetate solution (0.1moldm(-3), pH=6.5) and MeOH/ACN (1:1, v/v). Colorants were detected at 450, 500, and 625nm wavelengths. The TLC method proved to be suitable for quantitative determination in relatively wide range of concentrations (20-300ng/band). Satisfactory values of limits of detection and quantitation were established for both techniques, although somewhat higher in the case of TLC, ranging from 2-10ng/band, that is, 3-20ng/band. Both methods can be applied with satisfactory precision and accuracy, with clear advantage of TLC for simultaneous analysis of six samples per hour.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography and Related Technologies
T1  - Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes
VL  - 36
IS  - 17
SP  - 2476
EP  - 2488
DO  - 10.1080/10826076.2013.790771
ER  - 

@article{
author = "Vlajkovic, Jelena and Andrić, Filip and Ristivojević, Petar and Radoičić, Aleksandra and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2013",
abstract = "A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance liquid chromatography (RP-HPLC) method. In the case of TLC method horizontal developing technique was employed using octadecyl-modified silica as a stationary phase and 0.5mol dm(-3) ammonium sulfate in 30% of ethanol-water solution as a mobile phase. HPLC analysis was carried out using XDB-C18 column and gradient of mixture of aqueous ammonium acetate solution (0.1moldm(-3), pH=6.5) and MeOH/ACN (1:1, v/v). Colorants were detected at 450, 500, and 625nm wavelengths. The TLC method proved to be suitable for quantitative determination in relatively wide range of concentrations (20-300ng/band). Satisfactory values of limits of detection and quantitation were established for both techniques, although somewhat higher in the case of TLC, ranging from 2-10ng/band, that is, 3-20ng/band. Both methods can be applied with satisfactory precision and accuracy, with clear advantage of TLC for simultaneous analysis of six samples per hour.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography and Related Technologies",
title = "Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes",
volume = "36",
number = "17",
pages = "2476-2488",
doi = "10.1080/10826076.2013.790771"
}

Vlajkovic, J., Andrić, F., Ristivojević, P., Radoičić, A., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2013). Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes. in Journal of Liquid Chromatography and Related Technologies
Taylor & Francis Inc, Philadelphia., 36(17), 2476-2488.
https://doi.org/10.1080/10826076.2013.790771

Vlajkovic J, Andrić F, Ristivojević P, Radoičić A, Tešić ŽL, Milojković-Opsenica D. Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes. in Journal of Liquid Chromatography and Related Technologies. 2013;36(17):2476-2488.
doi:10.1080/10826076.2013.790771 .

Vlajkovic, Jelena, Andrić, Filip, Ristivojević, Petar, Radoičić, Aleksandra, Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Development and Validation of a Tlc Method for the Analysis of Synthetic Food-Stuff Dyes" in Journal of Liquid Chromatography and Related Technologies, 36, no. 17 (2013):2476-2488,
https://doi.org/10.1080/10826076.2013.790771 . .