TY  - JOUR
AU  - Mandić, Boris
AU  - Vlajić, Marina D.
AU  - Trifunović, Snežana S.
AU  - Simić, Milena R.
AU  - Vujisić, Ljubodrag V.
AU  - Vučković, Ivan M.
AU  - Novaković, Miroslav M.
AU  - Nikolić-Mandić, Snežana D.
AU  - Tešević, Vele
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1692
AB  - Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge
VL  - 29
IS  - 9
SP  - 887
EP  - 890
DO  - 10.1080/14786419.2014.991929
ER  - 

@article{
author = "Mandić, Boris and Vlajić, Marina D. and Trifunović, Snežana S. and Simić, Milena R. and Vujisić, Ljubodrag V. and Vučković, Ivan M. and Novaković, Miroslav M. and Nikolić-Mandić, Snežana D. and Tešević, Vele and Vajs, Vlatka and Milosavljević, Slobodan M.",
year = "2015",
abstract = "Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge",
volume = "29",
number = "9",
pages = "887-890",
doi = "10.1080/14786419.2014.991929"
}

Mandić, B., Vlajić, M. D., Trifunović, S. S., Simić, M. R., Vujisić, L. V., Vučković, I. M., Novaković, M. M., Nikolić-Mandić, S. D., Tešević, V., Vajs, V.,& Milosavljević, S. M.. (2015). Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 29(9), 887-890.
https://doi.org/10.1080/14786419.2014.991929

Mandić B, Vlajić MD, Trifunović SS, Simić MR, Vujisić LV, Vučković IM, Novaković MM, Nikolić-Mandić SD, Tešević V, Vajs V, Milosavljević SM. Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge. in Natural Product Research. 2015;29(9):887-890.
doi:10.1080/14786419.2014.991929 .

Mandić, Boris, Vlajić, Marina D., Trifunović, Snežana S., Simić, Milena R., Vujisić, Ljubodrag V., Vučković, Ivan M., Novaković, Miroslav M., Nikolić-Mandić, Snežana D., Tešević, Vele, Vajs, Vlatka, Milosavljević, Slobodan M., "Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge" in Natural Product Research, 29, no. 9 (2015):887-890,
https://doi.org/10.1080/14786419.2014.991929 . .

TY  - DATA
AU  - Mandić, Boris
AU  - Vlajić, Marina D.
AU  - Trifunović, Snežana S.
AU  - Simić, Milena R.
AU  - Vujisić, Ljubodrag V.
AU  - Vučković, Ivan M.
AU  - Novaković, Miroslav M.
AU  - Nikolić-Mandić, Snežana D.
AU  - Tešević, Vele
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3388
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Supplementary data for article: Mandić, B. M.; Vlajić, M. D.; Trifunović, S. S.; Simić, M. R.; Vujisić, L. V.; Vučković, I. M.; Novaković, M. M.; Nikolić-Mandić, S. D.; Tešević, V. V.; Vajs, V. V.; et al. Optimisation of Isolation Procedure for Pyrrolizidine Alkaloids from Rindera Umbellata Bunge. Natural Product Research 2015, 29 (9), 887–890. https://doi.org/10.1080/14786419.2014.991929
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3388
ER  - 

@misc{
author = "Mandić, Boris and Vlajić, Marina D. and Trifunović, Snežana S. and Simić, Milena R. and Vujisić, Ljubodrag V. and Vučković, Ivan M. and Novaković, Miroslav M. and Nikolić-Mandić, Snežana D. and Tešević, Vele and Vajs, Vlatka and Milosavljević, Slobodan M.",
year = "2015",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Supplementary data for article: Mandić, B. M.; Vlajić, M. D.; Trifunović, S. S.; Simić, M. R.; Vujisić, L. V.; Vučković, I. M.; Novaković, M. M.; Nikolić-Mandić, S. D.; Tešević, V. V.; Vajs, V. V.; et al. Optimisation of Isolation Procedure for Pyrrolizidine Alkaloids from Rindera Umbellata Bunge. Natural Product Research 2015, 29 (9), 887–890. https://doi.org/10.1080/14786419.2014.991929",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3388"
}

Mandić, B., Vlajić, M. D., Trifunović, S. S., Simić, M. R., Vujisić, L. V., Vučković, I. M., Novaković, M. M., Nikolić-Mandić, S. D., Tešević, V., Vajs, V.,& Milosavljević, S. M.. (2015). Supplementary data for article: Mandić, B. M.; Vlajić, M. D.; Trifunović, S. S.; Simić, M. R.; Vujisić, L. V.; Vučković, I. M.; Novaković, M. M.; Nikolić-Mandić, S. D.; Tešević, V. V.; Vajs, V. V.; et al. Optimisation of Isolation Procedure for Pyrrolizidine Alkaloids from Rindera Umbellata Bunge. Natural Product Research 2015, 29 (9), 887–890. https://doi.org/10.1080/14786419.2014.991929. in Natural Product Research
Taylor & Francis Ltd, Abingdon..
https://hdl.handle.net/21.15107/rcub_cherry_3388

Mandić B, Vlajić MD, Trifunović SS, Simić MR, Vujisić LV, Vučković IM, Novaković MM, Nikolić-Mandić SD, Tešević V, Vajs V, Milosavljević SM. Supplementary data for article: Mandić, B. M.; Vlajić, M. D.; Trifunović, S. S.; Simić, M. R.; Vujisić, L. V.; Vučković, I. M.; Novaković, M. M.; Nikolić-Mandić, S. D.; Tešević, V. V.; Vajs, V. V.; et al. Optimisation of Isolation Procedure for Pyrrolizidine Alkaloids from Rindera Umbellata Bunge. Natural Product Research 2015, 29 (9), 887–890. https://doi.org/10.1080/14786419.2014.991929. in Natural Product Research. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3388 .

Mandić, Boris, Vlajić, Marina D., Trifunović, Snežana S., Simić, Milena R., Vujisić, Ljubodrag V., Vučković, Ivan M., Novaković, Miroslav M., Nikolić-Mandić, Snežana D., Tešević, Vele, Vajs, Vlatka, Milosavljević, Slobodan M., "Supplementary data for article: Mandić, B. M.; Vlajić, M. D.; Trifunović, S. S.; Simić, M. R.; Vujisić, L. V.; Vučković, I. M.; Novaković, M. M.; Nikolić-Mandić, S. D.; Tešević, V. V.; Vajs, V. V.; et al. Optimisation of Isolation Procedure for Pyrrolizidine Alkaloids from Rindera Umbellata Bunge. Natural Product Research 2015, 29 (9), 887–890. https://doi.org/10.1080/14786419.2014.991929" in Natural Product Research (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3388 .