TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5874
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
VL  - 24
IS  - 3
SP  - 1903
DO  - 10.3390/ijms24031903
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study",
volume = "24",
number = "3",
pages = "1903",
doi = "10.3390/ijms24031903"
}

Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences
MDPI., 24(3), 1903.
https://doi.org/10.3390/ijms24031903

Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3):1903.
doi:10.3390/ijms24031903 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023):1903,
https://doi.org/10.3390/ijms24031903 . .

TY  - DATA
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Prđun, Saša
AU  - Bubalo, Dragan
AU  - Lušić, Dražen
AU  - Vahčić, Nada
AU  - Kopjar, Nevenka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5875
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study
VL  - 24
IS  - 3
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5875
ER  - 

@misc{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Prđun, Saša and Bubalo, Dragan and Lušić, Dražen and Vahčić, Nada and Kopjar, Nevenka",
year = "2023",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap–Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study",
volume = "24",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5875"
}

Jurič, A., Brčić Karačonji, I., Gašić, U. M., Milojković-Opsenica, D., Prđun, S., Bubalo, D., Lušić, D., Vahčić, N.,& Kopjar, N.. (2023). Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences
MDPI., 24(3).
https://hdl.handle.net/21.15107/rcub_cherry_5875

Jurič A, Brčić Karačonji I, Gašić UM, Milojković-Opsenica D, Prđun S, Bubalo D, Lušić D, Vahčić N, Kopjar N. Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study. in International Journal of Molecular Sciences. 2023;24(3).
https://hdl.handle.net/21.15107/rcub_cherry_5875 .

Jurič, Andreja, Brčić Karačonji, Irena, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Prđun, Saša, Bubalo, Dragan, Lušić, Dražen, Vahčić, Nada, Kopjar, Nevenka, "Supplementary material for: Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes—An In Vitro Study" in International Journal of Molecular Sciences, 24, no. 3 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5875 .

TY  - CONF
AU  - Jakanovski, Mihajlo V.
AU  - Dramićanin, Aleksandra M.
AU  - Horvacki, Nikola
AU  - Trifković, Jelena
AU  - Rašić, Dubravka
AU  - Brčić Karačonji, Irena
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5933
AB  - Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products
SP  - 73
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5933
ER  - 

@conference{
author = "Jakanovski, Mihajlo V. and Dramićanin, Aleksandra M. and Horvacki, Nikola and Trifković, Jelena and Rašić, Dubravka and Brčić Karačonji, Irena and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products",
pages = "73-74",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5933"
}

Jakanovski, M. V., Dramićanin, A. M., Horvacki, N., Trifković, J., Rašić, D., Brčić Karačonji, I.,& Milojković-Opsenica, D.. (2023). Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933

Jakanovski MV, Dramićanin AM, Horvacki N, Trifković J, Rašić D, Brčić Karačonji I, Milojković-Opsenica D. Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

Jakanovski, Mihajlo V., Dramićanin, Aleksandra M., Horvacki, Nikola, Trifković, Jelena, Rašić, Dubravka, Brčić Karačonji, Irena, Milojković-Opsenica, Dušanka, "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):73-74,
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

TY  - JOUR
AU  - Brčić-Karačonji, Irena
AU  - Jurica, Karlo
AU  - Gašić, Uroš M.
AU  - Dramićanin, Aleksandra M.
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5014
AB  - The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean
shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial,
antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the
presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total
phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected
at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid
chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was
determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of
64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside
and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were
the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and
fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed
that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate
samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of
67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA
for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 µmol Trolox equivalents
(TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves.
Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to
A. unedo as a promising health-promoting plant and natural food preservative.
PB  - MDPI
T2  - Plants
T1  - Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
VL  - 11
IS  - 1
SP  - 25
DO  - 10.3390/plants11010025
ER  - 

@article{
author = "Brčić-Karačonji, Irena and Jurica, Karlo and Gašić, Uroš M. and Dramićanin, Aleksandra M. and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean
shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial,
antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the
presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total
phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected
at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid
chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was
determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of
64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside
and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were
the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and
fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed
that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate
samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of
67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA
for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 µmol Trolox equivalents
(TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves.
Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to
A. unedo as a promising health-promoting plant and natural food preservative.",
publisher = "MDPI",
journal = "Plants",
title = "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits",
volume = "11",
number = "1",
pages = "25",
doi = "10.3390/plants11010025"
}

Brčić-Karačonji, I., Jurica, K., Gašić, U. M., Dramićanin, A. M., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2022). Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits. in Plants
MDPI., 11(1), 25.
https://doi.org/10.3390/plants11010025

Brčić-Karačonji I, Jurica K, Gašić UM, Dramićanin AM, Tešić ŽL, Milojković-Opsenica D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits. in Plants. 2022;11(1):25.
doi:10.3390/plants11010025 .

Brčić-Karačonji, Irena, Jurica, Karlo, Gašić, Uroš M., Dramićanin, Aleksandra M., Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits" in Plants, 11, no. 1 (2022):25,
https://doi.org/10.3390/plants11010025 . .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Žunec, Suzana
AU  - Katić, Anja
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Kopjar, Nevenka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5199
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine
in the Mediterranean area, especially in the treatment of wounds, burns and infections. In
this study, we investigated the cyto-/genoprotective properties of STH at cell level following
short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes
(PBL) were selected as model cells. Phenolics in STH were determined using an ultrahigh-
performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid
mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a
gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with
STH before and after irradiation was assessed by alkaline comet assay, while their viability
was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive
substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied
as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted
the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress.
Post-radiation treatment with STH was not as beneficial. Protective effects could be associated
to the complex phytochemical profile of STH and the high content of HGA (306.8mg/
kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH
prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms,
including intracellular antioxidative defence and DNA repair systems that helped pretreated
cells to counteract harmful exposures more efficiently.
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
SP  - 1
EP  - 10
DO  - 10.1080/00218839.2022.2047421
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Žunec, Suzana and Katić, Anja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Kopjar, Nevenka",
year = "2022",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine
in the Mediterranean area, especially in the treatment of wounds, burns and infections. In
this study, we investigated the cyto-/genoprotective properties of STH at cell level following
short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes
(PBL) were selected as model cells. Phenolics in STH were determined using an ultrahigh-
performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid
mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a
gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with
STH before and after irradiation was assessed by alkaline comet assay, while their viability
was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive
substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied
as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted
the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress.
Post-radiation treatment with STH was not as beneficial. Protective effects could be associated
to the complex phytochemical profile of STH and the high content of HGA (306.8mg/
kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH
prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms,
including intracellular antioxidative defence and DNA repair systems that helped pretreated
cells to counteract harmful exposures more efficiently.",
publisher = "Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro",
pages = "1-10",
doi = "10.1080/00218839.2022.2047421"
}

Jurič, A., Brčić Karačonji, I., Žunec, S., Katić, A., Gašić, U. M., Milojković-Opsenica, D.,& Kopjar, N.. (2022). Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research
Taylor and Francis., 1-10.
https://doi.org/10.1080/00218839.2022.2047421

Jurič A, Brčić Karačonji I, Žunec S, Katić A, Gašić UM, Milojković-Opsenica D, Kopjar N. Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research. 2022;:1-10.
doi:10.1080/00218839.2022.2047421 .

Jurič, Andreja, Brčić Karačonji, Irena, Žunec, Suzana, Katić, Anja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Kopjar, Nevenka, "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro" in Journal of Apicultural Research (2022):1-10,
https://doi.org/10.1080/00218839.2022.2047421 . .

TY  - DATA
AU  - Brčić-Karačonji, Irena
AU  - Jurica, Karlo
AU  - Gašić, Uroš M.
AU  - Dramićanin, Aleksandra M.
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5017
PB  - MDPI
T2  - Plants
T1  - Supplementary data for the article: Brčić-Karačonji, I.; Jurica, K.; Gašić, U. M.; Dramićanin, A. M.; Tešić, Ž. L.; Milojković-Opsenica, D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus Unedo L.) Leaves and Fruits. Plants 2021, 11 (25). https://doi.org/10.3390/plants11010025.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5017
ER  - 

@misc{
author = "Brčić-Karačonji, Irena and Jurica, Karlo and Gašić, Uroš M. and Dramićanin, Aleksandra M. and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2021",
publisher = "MDPI",
journal = "Plants",
title = "Supplementary data for the article: Brčić-Karačonji, I.; Jurica, K.; Gašić, U. M.; Dramićanin, A. M.; Tešić, Ž. L.; Milojković-Opsenica, D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus Unedo L.) Leaves and Fruits. Plants 2021, 11 (25). https://doi.org/10.3390/plants11010025.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5017"
}

Brčić-Karačonji, I., Jurica, K., Gašić, U. M., Dramićanin, A. M., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2021). Supplementary data for the article: Brčić-Karačonji, I.; Jurica, K.; Gašić, U. M.; Dramićanin, A. M.; Tešić, Ž. L.; Milojković-Opsenica, D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus Unedo L.) Leaves and Fruits. Plants 2021, 11 (25). https://doi.org/10.3390/plants11010025.. in Plants
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_5017

Brčić-Karačonji I, Jurica K, Gašić UM, Dramićanin AM, Tešić ŽL, Milojković-Opsenica D. Supplementary data for the article: Brčić-Karačonji, I.; Jurica, K.; Gašić, U. M.; Dramićanin, A. M.; Tešić, Ž. L.; Milojković-Opsenica, D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus Unedo L.) Leaves and Fruits. Plants 2021, 11 (25). https://doi.org/10.3390/plants11010025.. in Plants. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_5017 .

Brčić-Karačonji, Irena, Jurica, Karlo, Gašić, Uroš M., Dramićanin, Aleksandra M., Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Supplementary data for the article: Brčić-Karačonji, I.; Jurica, K.; Gašić, U. M.; Dramićanin, A. M.; Tešić, Ž. L.; Milojković-Opsenica, D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus Unedo L.) Leaves and Fruits. Plants 2021, 11 (25). https://doi.org/10.3390/plants11010025." in Plants (2021),
https://hdl.handle.net/21.15107/rcub_cherry_5017 .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Gašić, Gašić
AU  - Brčić-Karačonji, Irena
AU  - Jurica, Karlo
AU  - Milojković-Opsenica, Dušanka
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3952
AB  - Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - The phenolic profile of strawberry tree (Arbutus unedo L.) honey
VL  - 85
IS  - 3
SP  - 1
EP  - 10
DO  - 10.2298/JSC191217018J
ER  - 

@article{
author = "Jurič, Andreja and Gašić, Gašić and Brčić-Karačonji, Irena and Jurica, Karlo and Milojković-Opsenica, Dušanka",
year = "2020",
abstract = "Despite of the many beneficial health effects of strawberry tree (Arbutus unedo L.) honey due to its strong antioxidant activity derived mostly from polyphenols, a detailed phenolic profile has not been previously studied. The aims of this study were to identify the phenolic compounds, determine the total phenolic content (TPC) and evaluate the radical scavenging activity (RSA) of strawberry tree honey from south Croatia. Fifty-two polyphenolics (twenty-seven phenolic acids and twenty-five flavonoids) were identified using ultra-high-performance liquid chromatograph (UHPLC) coupled to a hybrid mass spectrometer (LTQ Orbitrap MS). Our overall results point to higher TPC (1038 mg gallic acid equivalents per kg of honey) and stronger RSA (3.32 mmol Trolox equivalents per kg of honey) compared to other monofloral honeys. Due to the presence of large quantities of polyphenolic compounds, strawberry tree honey may have great potential as a health promoting food.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "The phenolic profile of strawberry tree (Arbutus unedo L.) honey",
volume = "85",
number = "3",
pages = "1-10",
doi = "10.2298/JSC191217018J"
}

Jurič, A., Gašić, G., Brčić-Karačonji, I., Jurica, K.,& Milojković-Opsenica, D.. (2020). The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 85(3), 1-10.
https://doi.org/10.2298/JSC191217018J

Jurič A, Gašić G, Brčić-Karačonji I, Jurica K, Milojković-Opsenica D. The phenolic profile of strawberry tree (Arbutus unedo L.) honey. in Journal of the Serbian Chemical Society. 2020;85(3):1-10.
doi:10.2298/JSC191217018J .

Jurič, Andreja, Gašić, Gašić, Brčić-Karačonji, Irena, Jurica, Karlo, Milojković-Opsenica, Dušanka, "The phenolic profile of strawberry tree (Arbutus unedo L.) honey" in Journal of the Serbian Chemical Society, 85, no. 3 (2020):1-10,
https://doi.org/10.2298/JSC191217018J . .

TY  - JOUR
AU  - Jurica, Karlo
AU  - Brčić-Karačonji, Irena
AU  - Mikolić, Anja
AU  - Milojković-Opsenica, Dušanka
AU  - Benković, Vesna
AU  - Kopjar, Nevenka
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2194
AB  - Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.
PB  - Springer, Dordrecht
T2  - Cytotechnology
T1  - In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes
VL  - 70
IS  - 4
SP  - 1261
EP  - 1278
DO  - 10.1007/s10616-018-0218-4
ER  - 

@article{
author = "Jurica, Karlo and Brčić-Karačonji, Irena and Mikolić, Anja and Milojković-Opsenica, Dušanka and Benković, Vesna and Kopjar, Nevenka",
year = "2018",
abstract = "Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.",
publisher = "Springer, Dordrecht",
journal = "Cytotechnology",
title = "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes",
volume = "70",
number = "4",
pages = "1261-1278",
doi = "10.1007/s10616-018-0218-4"
}

Jurica, K., Brčić-Karačonji, I., Mikolić, A., Milojković-Opsenica, D., Benković, V.,& Kopjar, N.. (2018). In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes. in Cytotechnology
Springer, Dordrecht., 70(4), 1261-1278.
https://doi.org/10.1007/s10616-018-0218-4

Jurica K, Brčić-Karačonji I, Mikolić A, Milojković-Opsenica D, Benković V, Kopjar N. In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes. in Cytotechnology. 2018;70(4):1261-1278.
doi:10.1007/s10616-018-0218-4 .

Jurica, Karlo, Brčić-Karačonji, Irena, Mikolić, Anja, Milojković-Opsenica, Dušanka, Benković, Vesna, Kopjar, Nevenka, "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes" in Cytotechnology, 70, no. 4 (2018):1261-1278,
https://doi.org/10.1007/s10616-018-0218-4 . .

TY  - JOUR
AU  - Jurica, Karlo
AU  - Brčić-Karačonji, Irena
AU  - Šegan, Sandra B.
AU  - Milojković-Opsenica, Dušanka
AU  - Kremer, Dario
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2009
AB  - The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2 gt 0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD lt 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry
VL  - 66
IS  - 3
SP  - 197
EP  - 202
DO  - 10.1515/aiht-2015-66-2696
ER  - 

@article{
author = "Jurica, Karlo and Brčić-Karačonji, Irena and Šegan, Sandra B. and Milojković-Opsenica, Dušanka and Kremer, Dario",
year = "2015",
abstract = "The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2 gt 0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD lt 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry",
volume = "66",
number = "3",
pages = "197-202",
doi = "10.1515/aiht-2015-66-2696"
}

Jurica, K., Brčić-Karačonji, I., Šegan, S. B., Milojković-Opsenica, D.,& Kremer, D.. (2015). Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv za higijenu rada i toksikologiju
Inst Medical Research & Occupational Health, Zagreb., 66(3), 197-202.
https://doi.org/10.1515/aiht-2015-66-2696

Jurica K, Brčić-Karačonji I, Šegan SB, Milojković-Opsenica D, Kremer D. Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv za higijenu rada i toksikologiju. 2015;66(3):197-202.
doi:10.1515/aiht-2015-66-2696 .

Jurica, Karlo, Brčić-Karačonji, Irena, Šegan, Sandra B., Milojković-Opsenica, Dušanka, Kremer, Dario, "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry" in Arhiv za higijenu rada i toksikologiju, 66, no. 3 (2015):197-202,
https://doi.org/10.1515/aiht-2015-66-2696 . .