TY  - JOUR
AU  - Tosti, Tomislav
AU  - Natić, Maja
AU  - Smolinski, A.
AU  - Milić, Dragana
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1196
AB  - The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1: 9 (v/v). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal-and reversed-phase systems used, retention parameters were investigated by principal component analysis and cluster analysis.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Chromatographica
T1  - Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography
VL  - 23
IS  - 3
SP  - 429
EP  - 445
DO  - 10.1556/AChrom.23.2011.3.5
ER  - 

@article{
author = "Tosti, Tomislav and Natić, Maja and Smolinski, A. and Milić, Dragana and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj.",
year = "2011",
abstract = "The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1: 9 (v/v). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal-and reversed-phase systems used, retention parameters were investigated by principal component analysis and cluster analysis.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Chromatographica",
title = "Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography",
volume = "23",
number = "3",
pages = "429-445",
doi = "10.1556/AChrom.23.2011.3.5"
}

Tosti, T., Natić, M., Smolinski, A., Milić, D., Milojković-Opsenica, D.,& Tešić, Ž. Lj.. (2011). Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography. in Acta Chromatographica
Akademiai Kiado Rt, Budapest., 23(3), 429-445.
https://doi.org/10.1556/AChrom.23.2011.3.5

Tosti T, Natić M, Smolinski A, Milić D, Milojković-Opsenica D, Tešić ŽL. Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography. in Acta Chromatographica. 2011;23(3):429-445.
doi:10.1556/AChrom.23.2011.3.5 .

Tosti, Tomislav, Natić, Maja, Smolinski, A., Milić, Dragana, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., "Study of Retention of 31 Polyoxygenated Steroids by Normal- and Reversed-Phase Thin-Layer Chromatography" in Acta Chromatographica, 23, no. 3 (2011):429-445,
https://doi.org/10.1556/AChrom.23.2011.3.5 . .