TY  - JOUR
AU  - Čavić, Milena
AU  - Grozdanović, Milica M.
AU  - Bajić, Aleksandar
AU  - Jankovic, Radmila
AU  - Andjus, Pavle R.
AU  - Gavrović-Jankulović, Marija
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1866
AB  - Actinidin, a kiwifruit cysteine protease, is a marker allergen for genuine sensitization to this food allergen source. Inhalatory cysteine proteases have the capacity for disruption of tight junctions (TJs) enhancing the permeability of the bronchial epithelium. No such properties have been reported for allergenic food proteases so far. The aim was to determine the effect of actinidin on the integrity of T84 monolayers by evaluating its action on the TJ protein occludin. Immunoblot and immunofluorescence were employed for the detection of occludin protein alterations. Gene expression was evaluated by RT-PCR. Breach of occludin network was assessed by measuring transepithelial resistance, blue dextran leakage and passage of allergens from the apical to basolateral compartment. Actinidin exerted direct proteolytic cleavage of occludin; no alteration of occludin gene expression was detected. There was a reduction of occludin staining upon actinidin treatment as a consequence of its degradation and dispersion within the membrane. There was an increase in permeability of the T84 monolayer resulting in reduced transepithelial resistance, blue dextran leakage and passage of allergens actinidin and thaumatin-like protein from the apical to basolateral compartment. Opening of TJs by actinidin may increase intestinal permeability and contribute to the process of sensitization in kiwifruit allergy. (C) 2014 Elsevier Ltd. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells
VL  - 72
SP  - 61
EP  - 68
DO  - 10.1016/j.fct.2014.07.012
ER  - 

@article{
author = "Čavić, Milena and Grozdanović, Milica M. and Bajić, Aleksandar and Jankovic, Radmila and Andjus, Pavle R. and Gavrović-Jankulović, Marija",
year = "2014",
abstract = "Actinidin, a kiwifruit cysteine protease, is a marker allergen for genuine sensitization to this food allergen source. Inhalatory cysteine proteases have the capacity for disruption of tight junctions (TJs) enhancing the permeability of the bronchial epithelium. No such properties have been reported for allergenic food proteases so far. The aim was to determine the effect of actinidin on the integrity of T84 monolayers by evaluating its action on the TJ protein occludin. Immunoblot and immunofluorescence were employed for the detection of occludin protein alterations. Gene expression was evaluated by RT-PCR. Breach of occludin network was assessed by measuring transepithelial resistance, blue dextran leakage and passage of allergens from the apical to basolateral compartment. Actinidin exerted direct proteolytic cleavage of occludin; no alteration of occludin gene expression was detected. There was a reduction of occludin staining upon actinidin treatment as a consequence of its degradation and dispersion within the membrane. There was an increase in permeability of the T84 monolayer resulting in reduced transepithelial resistance, blue dextran leakage and passage of allergens actinidin and thaumatin-like protein from the apical to basolateral compartment. Opening of TJs by actinidin may increase intestinal permeability and contribute to the process of sensitization in kiwifruit allergy. (C) 2014 Elsevier Ltd. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells",
volume = "72",
pages = "61-68",
doi = "10.1016/j.fct.2014.07.012"
}

Čavić, M., Grozdanović, M. M., Bajić, A., Jankovic, R., Andjus, P. R.,& Gavrović-Jankulović, M.. (2014). The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 72, 61-68.
https://doi.org/10.1016/j.fct.2014.07.012

Čavić M, Grozdanović MM, Bajić A, Jankovic R, Andjus PR, Gavrović-Jankulović M. The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells. in Food and Chemical Toxicology. 2014;72:61-68.
doi:10.1016/j.fct.2014.07.012 .

Čavić, Milena, Grozdanović, Milica M., Bajić, Aleksandar, Jankovic, Radmila, Andjus, Pavle R., Gavrović-Jankulović, Marija, "The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells" in Food and Chemical Toxicology, 72 (2014):61-68,
https://doi.org/10.1016/j.fct.2014.07.012 . .

TY  - JOUR
AU  - Čavić, Milena
AU  - Grozdanović, Milica
AU  - Bajić, Aleksandar
AU  - Srdić-Rajić, Tatjana
AU  - Andjus, Pavle R.
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1284
AB  - Actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin's action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the development of kiwifruit allergy and other intestinal disorders.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells
VL  - 77
SP  - 46
EP  - 52
DO  - 10.1016/j.phytochem.2011.12.014
ER  - 

@article{
author = "Čavić, Milena and Grozdanović, Milica and Bajić, Aleksandar and Srdić-Rajić, Tatjana and Andjus, Pavle R. and Gavrović-Jankulović, Marija",
year = "2012",
abstract = "Actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin's action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the development of kiwifruit allergy and other intestinal disorders.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells",
volume = "77",
pages = "46-52",
doi = "10.1016/j.phytochem.2011.12.014"
}

Čavić, M., Grozdanović, M., Bajić, A., Srdić-Rajić, T., Andjus, P. R.,& Gavrović-Jankulović, M.. (2012). Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells. in Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford., 77, 46-52.
https://doi.org/10.1016/j.phytochem.2011.12.014

Čavić M, Grozdanović M, Bajić A, Srdić-Rajić T, Andjus PR, Gavrović-Jankulović M. Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells. in Phytochemistry. 2012;77:46-52.
doi:10.1016/j.phytochem.2011.12.014 .

Čavić, Milena, Grozdanović, Milica, Bajić, Aleksandar, Srdić-Rajić, Tatjana, Andjus, Pavle R., Gavrović-Jankulović, Marija, "Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells" in Phytochemistry, 77 (2012):46-52,
https://doi.org/10.1016/j.phytochem.2011.12.014 . .