Mosić, Mirjana D.

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  • Mosić, Mirjana D. (6)
  • Mosić, Mirjana (2)

Author's Bibliography

Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics

Mosić, Mirjana D.; Trifković, Jelena; Ristivojević, Petar; Milojković-Opsenica, Dušanka

(Wiley, 2023)

TY  - JOUR
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5876
AB  - The aim of this study is to develop a rapid, effect-directed screening method for quality assessment of bee pollen-honey mixtures. The comparative antioxidant potential and phenolic content of honey, bee pollen, and the bee pollen-honey mixtures, was performed using spectrophotometry. The total phenolic content and antioxidative activity of bee pollen-honey mixtures with 20 % bee pollen share were in the range 3.03–3.11 mg GAE/g, and 6.02–6.96 mmol TE/kg, respectively, while mixtures with 30 % bee pollen share contained 3.92–4.18 mg GAE/g, and 9.69–10.11 mmol TE/kg. Chromatographic fingerprint of bee pollen-honey mixtures was performed by high-performance thin-layer chromatography with conditions developed by authors and reported for the first time. Fingerprint analysis hyphenated with chemometrics enabled authenticity assessments of honey in mixtures. Results indicate that bee pollen-honey mixtures represent a food with highly, both, nutritious characteristics and health-promoting effect.
PB  - Wiley
T2  - Chemistry & Biodiversity
T2  - Chemistry & Biodiversity
T1  - Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics
VL  - 20
IS  - 3
SP  - e202201141
DO  - 10.1002/cbdv.202201141
ER  - 
@article{
author = "Mosić, Mirjana D. and Trifković, Jelena and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "The aim of this study is to develop a rapid, effect-directed screening method for quality assessment of bee pollen-honey mixtures. The comparative antioxidant potential and phenolic content of honey, bee pollen, and the bee pollen-honey mixtures, was performed using spectrophotometry. The total phenolic content and antioxidative activity of bee pollen-honey mixtures with 20 % bee pollen share were in the range 3.03–3.11 mg GAE/g, and 6.02–6.96 mmol TE/kg, respectively, while mixtures with 30 % bee pollen share contained 3.92–4.18 mg GAE/g, and 9.69–10.11 mmol TE/kg. Chromatographic fingerprint of bee pollen-honey mixtures was performed by high-performance thin-layer chromatography with conditions developed by authors and reported for the first time. Fingerprint analysis hyphenated with chemometrics enabled authenticity assessments of honey in mixtures. Results indicate that bee pollen-honey mixtures represent a food with highly, both, nutritious characteristics and health-promoting effect.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity, Chemistry & Biodiversity",
title = "Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics",
volume = "20",
number = "3",
pages = "e202201141",
doi = "10.1002/cbdv.202201141"
}
Mosić, M. D., Trifković, J., Ristivojević, P.,& Milojković-Opsenica, D.. (2023). Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics. in Chemistry & Biodiversity
Wiley., 20(3), e202201141.
https://doi.org/10.1002/cbdv.202201141
Mosić MD, Trifković J, Ristivojević P, Milojković-Opsenica D. Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics. in Chemistry & Biodiversity. 2023;20(3):e202201141.
doi:10.1002/cbdv.202201141 .
Mosić, Mirjana D., Trifković, Jelena, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Quality Assessment of Bee Pollen-Honey Mixtures Using Thin-Layer Chromatography in Combination with Chemometrics" in Chemistry & Biodiversity, 20, no. 3 (2023):e202201141,
https://doi.org/10.1002/cbdv.202201141 . .
1

The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process

Momirović, Nevena; Nikolić, Dragan; Jakanovski, Mihajlo V.; Drami, Aleksandra; Mosić, Mirjana; Momirović, Nebojša; Milojković-Opsenica, Dušanka

(Beograd : Srpsko hemijsko društvo, 2023)

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Drami, Aleksandra
AU  - Mosić, Mirjana
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5974
AB  - Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).

Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.

Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.

References:
[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).
[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process
SP  - 175
EP  - 175
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5974
ER  - 
@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Drami, Aleksandra and Mosić, Mirjana and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).

Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.

Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.

References:
[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).
[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process",
pages = "175-175",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5974"
}
Momirović, N., Nikolić, D., Jakanovski, M. V., Drami, A., Mosić, M., Momirović, N.,& Milojković-Opsenica, D.. (2023). The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 175-175.
https://hdl.handle.net/21.15107/rcub_cherry_5974
Momirović N, Nikolić D, Jakanovski MV, Drami A, Mosić M, Momirović N, Milojković-Opsenica D. The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:175-175.
https://hdl.handle.net/21.15107/rcub_cherry_5974 .
Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Drami, Aleksandra, Mosić, Mirjana, Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):175-175,
https://hdl.handle.net/21.15107/rcub_cherry_5974 .

The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process

Momirović, Nevena; Nikolić, Dragan; Jakanovski, Mihajlo V.; Drami, Aleksandra; Mosić, Mirjana; Momirović, Nebojša; Milojković-Opsenica, Dušanka

(Beograd : Srpsko hemijsko društvo, 2023)

TY  - CONF
AU  - Momirović, Nevena
AU  - Nikolić, Dragan
AU  - Jakanovski, Mihajlo V.
AU  - Drami, Aleksandra
AU  - Mosić, Mirjana
AU  - Momirović, Nebojša
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5975
AB  - Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.References:[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5975
ER  - 
@conference{
author = "Momirović, Nevena and Nikolić, Dragan and Jakanovski, Mihajlo V. and Drami, Aleksandra and Mosić, Mirjana and Momirović, Nebojša and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Besides being one of the most commercially grown berry fruits, strawberries (Fragaria x annanassa Duch.) are known for their nutritional value, richness in polyphenols, and antioxidant capacity. New directions of strawberry breeding and selection are set towards premium fruit quality, high sugars content, and desirable sugars/total acids ratio (sweet index, SI), which represent some of the main prerequisites for selecting new breeding materials [1]. With this intention, a set of 24 strawberry genotypes was cultivated, including 12 parental varieties and 12 of their descendants - perspective candidates obtained by crossing the parental varieties mentioned above. A total of eleven sugars were quantified using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Results implied that glucose, sorbitol, sucrose, and melibiose content were the most important sugars in separating the fruits of parental varieties and their progeny [2]. Results showed that old, parental varieties had a significantly higher content of glucose and sucrose, while new perspective genotypes had higher sorbitol and melibiose content (Fig.2). PCA analysis confirmed that parental (samples 1-12) and descendant genotypes (samples 13-24), could be discriminated according to sugars profile i.e. that these four sugars have an effect on their differentiation (Fig.1). This was in accordance with Mann-Whitney U test results (Fig.2).Fig.1. Principal Component Analysis - The difference between parental and descendant genotypes; score plot (A) – parental samples (PS) 1-12, descendant (DS) samples 13-24, and loading plot (B) – sugar components.Acknowledgments: This work has been supported by the Ministry of Education, Science, Technological Development and Innovation of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288., as well as company “Zeleni hit d.o.o.” from Belgrade, Serbia and “BerryLab” breeding consortium from Italy.References:[1]	L. Mazzoni, L. di Vittori, F. Balducci, T.Y. Forbes-Hernandez, F. Giamperi, M. Battino, B. Mezzeti, F. Capocasa, Scientia Horticulturae, 261: 108945 (2019).[2]	M. Fotirić Akšić, T. Tosti, M. Sredojević, J. Milivojević, M. Meland, M. Natić, Plants, 8: 205 (2019).",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5975"
}
Momirović, N., Nikolić, D., Jakanovski, M. V., Drami, A., Mosić, M., Momirović, N.,& Milojković-Opsenica, D.. (2023). The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_cherry_5975
Momirović N, Nikolić D, Jakanovski MV, Drami A, Mosić M, Momirović N, Milojković-Opsenica D. The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_5975 .
Momirović, Nevena, Nikolić, Dragan, Jakanovski, Mihajlo V., Drami, Aleksandra, Mosić, Mirjana, Momirović, Nebojša, Milojković-Opsenica, Dušanka, "The sugars content of parental and new perspective descendant strawberry genotypes potential approach for the future selection process" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5975 .

Standard methods for pollen research

Campos, Maria G.; Anjos, Ofélia; Chica, Manuel; Campoy, Pascual; Nozkova, Janka; Almaraz-Abarca, Norma; Barreto, Lidia M. R. C.; Nordi, João Carlos; Estevinho, Leticia M.; Pascoal, Ananias; Paula, Vanessa Branco; Chopina, Altino; Dias, Luis G.; Tešić, Živoslav Lj.; Mosić, Mirjana D.; Kostić, Aleksandar Ž.; Pešić, Mirjana B.; Milojković-Opsenica, Dušanka; Sickel, Wiebke; Ankenbrand, Markus J.; Grimmer, Gudrun; Steffan-Dewenter, Ingolf; Keller, Alexander; Förster, Frank; Tananaki, Chrysoula H.; Liolios, Vasilios; Kanelis, Dimitrios; Rodopoulou, Maria-Anna; Thrasyvoulou, Andreas; Paulo, Luísa; Kast, Christina; Lucchetti, Matteo A.; Glauser, Gaëtan; Lokutova, Olena; de Almeida-Muradian, Ligia Bicudo; Szczęsna, Teresa; Carreck, Norman L.

(Taylor & Francis, 2021)

TY  - JOUR
AU  - Campos, Maria G.
AU  - Anjos, Ofélia
AU  - Chica, Manuel
AU  - Campoy, Pascual
AU  - Nozkova, Janka
AU  - Almaraz-Abarca, Norma
AU  - Barreto, Lidia M. R. C.
AU  - Nordi, João Carlos
AU  - Estevinho, Leticia M.
AU  - Pascoal, Ananias
AU  - Paula, Vanessa Branco
AU  - Chopina, Altino
AU  - Dias, Luis G.
AU  - Tešić, Živoslav Lj.
AU  - Mosić, Mirjana D.
AU  - Kostić, Aleksandar Ž.
AU  - Pešić, Mirjana B.
AU  - Milojković-Opsenica, Dušanka
AU  - Sickel, Wiebke
AU  - Ankenbrand, Markus J.
AU  - Grimmer, Gudrun
AU  - Steffan-Dewenter, Ingolf
AU  - Keller, Alexander
AU  - Förster, Frank
AU  - Tananaki, Chrysoula H.
AU  - Liolios, Vasilios
AU  - Kanelis, Dimitrios
AU  - Rodopoulou, Maria-Anna
AU  - Thrasyvoulou, Andreas
AU  - Paulo, Luísa
AU  - Kast, Christina
AU  - Lucchetti, Matteo A.
AU  - Glauser, Gaëtan
AU  - Lokutova, Olena
AU  - de Almeida-Muradian, Ligia Bicudo
AU  - Szczęsna, Teresa
AU  - Carreck, Norman L.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4683
AB  - “Bee pollen” is pollen collected from flowers by honey bees. It is used by the bees to nourish themselves, mainly by providing royal jelly and brood food, but it is also used for human nutrition. For the latter purpose, it is collected at the hive entrance as pellets that the bees bring to the hive. Bee pollen has diverse bioactivities, and thus has been used as a health food, and even as medication in some countries. In this paper, we provide standard methods for carrying out research on bee pollen. First, we introduce a method for the production and storage of bee pollen which assures quality of the product. Routine methods are then provided for the identification of the pollen’s floral sources, and determination of the more important quality criteria such as water content and content of proteins, carbohydrates, fatty acids, vitamins, alkaloids, phenolic and polyphenolic compounds. Finally, methods are described for the determination of some important bioactivities of bee pollen such as its antioxidant, anti-inflammatory, antimicrobial and antimutagenic properties. Métodos estándar Para la investigación del polenEl "polen de abeja" es el polen recogido de las flores por las abejas melíferas. El polen de abeja es utilizado para nutrir a las propias abejas, principalmente para proporcionar jalea real y alimento para las crías, pero también se utiliza para la nutrición humana. Para este último fin, se recoge en la entrada de la colmena en forma de gránulos que las abejas llevan a la colmena. El polen de abeja tiene diversas bioactividades, por lo que se hautilizado como alimento para la salud, e incluso como medicamento en algunos países. En este artículo, proporcionamos métodos estándar para llevar a cabo investigaciones sobre el polen de abeja. En primer lugar, presentamos un método de producción y almacenamiento de polen de abeja que garantiza la calidad del producto. A continuación, se ofrecen métodos de rutina para la identificación de las fuentes florales del polen y la determinación de los criterios de calidad más importantes, como el contenido de agua y de proteínas, carbohidratos, ácidos grasos, vitaminas, alcaloides y compuestos fenólicos y polifenólicos. Por último, se describen métodos para la determinación de algunas bioactividades importantes del polen de abeja, como sus propiedades antioxidantes, antiinflamatorias, antimicrobianas y antimutagénicas.
PB  - Taylor & Francis
T2  - Journal of Apicultural Research
T1  - Standard methods for pollen research
VL  - 60
IS  - 4
SP  - 1
EP  - 109
DO  - 10.1080/00218839.2021.1948240
ER  - 
@article{
author = "Campos, Maria G. and Anjos, Ofélia and Chica, Manuel and Campoy, Pascual and Nozkova, Janka and Almaraz-Abarca, Norma and Barreto, Lidia M. R. C. and Nordi, João Carlos and Estevinho, Leticia M. and Pascoal, Ananias and Paula, Vanessa Branco and Chopina, Altino and Dias, Luis G. and Tešić, Živoslav Lj. and Mosić, Mirjana D. and Kostić, Aleksandar Ž. and Pešić, Mirjana B. and Milojković-Opsenica, Dušanka and Sickel, Wiebke and Ankenbrand, Markus J. and Grimmer, Gudrun and Steffan-Dewenter, Ingolf and Keller, Alexander and Förster, Frank and Tananaki, Chrysoula H. and Liolios, Vasilios and Kanelis, Dimitrios and Rodopoulou, Maria-Anna and Thrasyvoulou, Andreas and Paulo, Luísa and Kast, Christina and Lucchetti, Matteo A. and Glauser, Gaëtan and Lokutova, Olena and de Almeida-Muradian, Ligia Bicudo and Szczęsna, Teresa and Carreck, Norman L.",
year = "2021",
abstract = "“Bee pollen” is pollen collected from flowers by honey bees. It is used by the bees to nourish themselves, mainly by providing royal jelly and brood food, but it is also used for human nutrition. For the latter purpose, it is collected at the hive entrance as pellets that the bees bring to the hive. Bee pollen has diverse bioactivities, and thus has been used as a health food, and even as medication in some countries. In this paper, we provide standard methods for carrying out research on bee pollen. First, we introduce a method for the production and storage of bee pollen which assures quality of the product. Routine methods are then provided for the identification of the pollen’s floral sources, and determination of the more important quality criteria such as water content and content of proteins, carbohydrates, fatty acids, vitamins, alkaloids, phenolic and polyphenolic compounds. Finally, methods are described for the determination of some important bioactivities of bee pollen such as its antioxidant, anti-inflammatory, antimicrobial and antimutagenic properties. Métodos estándar Para la investigación del polenEl "polen de abeja" es el polen recogido de las flores por las abejas melíferas. El polen de abeja es utilizado para nutrir a las propias abejas, principalmente para proporcionar jalea real y alimento para las crías, pero también se utiliza para la nutrición humana. Para este último fin, se recoge en la entrada de la colmena en forma de gránulos que las abejas llevan a la colmena. El polen de abeja tiene diversas bioactividades, por lo que se hautilizado como alimento para la salud, e incluso como medicamento en algunos países. En este artículo, proporcionamos métodos estándar para llevar a cabo investigaciones sobre el polen de abeja. En primer lugar, presentamos un método de producción y almacenamiento de polen de abeja que garantiza la calidad del producto. A continuación, se ofrecen métodos de rutina para la identificación de las fuentes florales del polen y la determinación de los criterios de calidad más importantes, como el contenido de agua y de proteínas, carbohidratos, ácidos grasos, vitaminas, alcaloides y compuestos fenólicos y polifenólicos. Por último, se describen métodos para la determinación de algunas bioactividades importantes del polen de abeja, como sus propiedades antioxidantes, antiinflamatorias, antimicrobianas y antimutagénicas.",
publisher = "Taylor & Francis",
journal = "Journal of Apicultural Research",
title = "Standard methods for pollen research",
volume = "60",
number = "4",
pages = "1-109",
doi = "10.1080/00218839.2021.1948240"
}
Campos, M. G., Anjos, O., Chica, M., Campoy, P., Nozkova, J., Almaraz-Abarca, N., Barreto, L. M. R. C., Nordi, J. C., Estevinho, L. M., Pascoal, A., Paula, V. B., Chopina, A., Dias, L. G., Tešić, Ž. Lj., Mosić, M. D., Kostić, A. Ž., Pešić, M. B., Milojković-Opsenica, D., Sickel, W., Ankenbrand, M. J., Grimmer, G., Steffan-Dewenter, I., Keller, A., Förster, F., Tananaki, C. H., Liolios, V., Kanelis, D., Rodopoulou, M., Thrasyvoulou, A., Paulo, L., Kast, C., Lucchetti, M. A., Glauser, G., Lokutova, O., de Almeida-Muradian, L. B., Szczęsna, T.,& Carreck, N. L.. (2021). Standard methods for pollen research. in Journal of Apicultural Research
Taylor & Francis., 60(4), 1-109.
https://doi.org/10.1080/00218839.2021.1948240
Campos MG, Anjos O, Chica M, Campoy P, Nozkova J, Almaraz-Abarca N, Barreto LMRC, Nordi JC, Estevinho LM, Pascoal A, Paula VB, Chopina A, Dias LG, Tešić ŽL, Mosić MD, Kostić AŽ, Pešić MB, Milojković-Opsenica D, Sickel W, Ankenbrand MJ, Grimmer G, Steffan-Dewenter I, Keller A, Förster F, Tananaki CH, Liolios V, Kanelis D, Rodopoulou M, Thrasyvoulou A, Paulo L, Kast C, Lucchetti MA, Glauser G, Lokutova O, de Almeida-Muradian LB, Szczęsna T, Carreck NL. Standard methods for pollen research. in Journal of Apicultural Research. 2021;60(4):1-109.
doi:10.1080/00218839.2021.1948240 .
Campos, Maria G., Anjos, Ofélia, Chica, Manuel, Campoy, Pascual, Nozkova, Janka, Almaraz-Abarca, Norma, Barreto, Lidia M. R. C., Nordi, João Carlos, Estevinho, Leticia M., Pascoal, Ananias, Paula, Vanessa Branco, Chopina, Altino, Dias, Luis G., Tešić, Živoslav Lj., Mosić, Mirjana D., Kostić, Aleksandar Ž., Pešić, Mirjana B., Milojković-Opsenica, Dušanka, Sickel, Wiebke, Ankenbrand, Markus J., Grimmer, Gudrun, Steffan-Dewenter, Ingolf, Keller, Alexander, Förster, Frank, Tananaki, Chrysoula H., Liolios, Vasilios, Kanelis, Dimitrios, Rodopoulou, Maria-Anna, Thrasyvoulou, Andreas, Paulo, Luísa, Kast, Christina, Lucchetti, Matteo A., Glauser, Gaëtan, Lokutova, Olena, de Almeida-Muradian, Ligia Bicudo, Szczęsna, Teresa, Carreck, Norman L., "Standard methods for pollen research" in Journal of Apicultural Research, 60, no. 4 (2021):1-109,
https://doi.org/10.1080/00218839.2021.1948240 . .
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Extraction as a Critical Step in Phytochemical Analysis

Mosić, Mirjana D.; Dramićanin, Aleksandra M.; Ristivojević, Petar; Milojković-Opsenica, Dušanka

(Oxford University Press, 2020)

TY  - JOUR
AU  - Mosić, Mirjana D.
AU  - Dramićanin, Aleksandra M.
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3915
AB  - Phytochemical analysis, the chemical investigation of plants, is a rapidly developing chemical discipline with various goals such as the determination of the substance classes and the isolation and qualitative and/or quantitative analyses of bioactive compounds or their structural analysis. The analysis of plants begins with optimizing extraction procedures because they play a key role in extraction outcomes, i.e., the isolation of bioactive compounds from natural products and the selection of the subsequent tests used in the analyses. The extraction is a critical step because it is necessary to extract the desired components of the complex natural matrix without destroying them. A wide range of techniques for the extraction of bioactive compounds from natural matrixes are currently available. This review aims to describe and compare the most commonly used methods, traditional/conventional and innovative/green. It will focus on the principles behind each method and on their strengths and limitations in order to help evaluate their suitability and economic feasibility, highlighting the advantages of new innovative techniques over conventional extraction procedures. Finally, the recent application of deep eutectic solvents as a new, eco-friendly method for the extraction of bioactive compounds from natural matrixes is also discussed.
PB  - Oxford University Press
T2  - Journal of AOAC International
T1  - Extraction as a Critical Step in Phytochemical Analysis
VL  - 103
DO  - 10.5740/jaoacint.19-0251
ER  - 
@article{
author = "Mosić, Mirjana D. and Dramićanin, Aleksandra M. and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2020",
abstract = "Phytochemical analysis, the chemical investigation of plants, is a rapidly developing chemical discipline with various goals such as the determination of the substance classes and the isolation and qualitative and/or quantitative analyses of bioactive compounds or their structural analysis. The analysis of plants begins with optimizing extraction procedures because they play a key role in extraction outcomes, i.e., the isolation of bioactive compounds from natural products and the selection of the subsequent tests used in the analyses. The extraction is a critical step because it is necessary to extract the desired components of the complex natural matrix without destroying them. A wide range of techniques for the extraction of bioactive compounds from natural matrixes are currently available. This review aims to describe and compare the most commonly used methods, traditional/conventional and innovative/green. It will focus on the principles behind each method and on their strengths and limitations in order to help evaluate their suitability and economic feasibility, highlighting the advantages of new innovative techniques over conventional extraction procedures. Finally, the recent application of deep eutectic solvents as a new, eco-friendly method for the extraction of bioactive compounds from natural matrixes is also discussed.",
publisher = "Oxford University Press",
journal = "Journal of AOAC International",
title = "Extraction as a Critical Step in Phytochemical Analysis",
volume = "103",
doi = "10.5740/jaoacint.19-0251"
}
Mosić, M. D., Dramićanin, A. M., Ristivojević, P.,& Milojković-Opsenica, D.. (2020). Extraction as a Critical Step in Phytochemical Analysis. in Journal of AOAC International
Oxford University Press., 103.
https://doi.org/10.5740/jaoacint.19-0251
Mosić MD, Dramićanin AM, Ristivojević P, Milojković-Opsenica D. Extraction as a Critical Step in Phytochemical Analysis. in Journal of AOAC International. 2020;103.
doi:10.5740/jaoacint.19-0251 .
Mosić, Mirjana D., Dramićanin, Aleksandra M., Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Extraction as a Critical Step in Phytochemical Analysis" in Journal of AOAC International, 103 (2020),
https://doi.org/10.5740/jaoacint.19-0251 . .
7
13
1
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8

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Mosić, Mirjana D.; Trifković, Jelena; Vovk, Irena; Gašić, Uroš M.; Tešić, Živoslav Lj.; Šikoparija, Branko; Milojković-Opsenica, Dušanka

(MDPI AG, 2019)

TY  - JOUR
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3774
PB  - MDPI AG
T2  - Biomolecules
T1  - Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen
VL  - 9
IS  - 12
DO  - 10.3390/biom9120783
ER  - 
@article{
author = "Mosić, Mirjana D. and Trifković, Jelena and Vovk, Irena and Gašić, Uroš M. and Tešić, Živoslav Lj. and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen",
volume = "9",
number = "12",
doi = "10.3390/biom9120783"
}
Mosić, M. D., Trifković, J., Vovk, I., Gašić, U. M., Tešić, Ž. Lj., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen. in Biomolecules
MDPI AG., 9(12).
https://doi.org/10.3390/biom9120783
Mosić MD, Trifković J, Vovk I, Gašić UM, Tešić ŽL, Šikoparija B, Milojković-Opsenica D. Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen. in Biomolecules. 2019;9(12).
doi:10.3390/biom9120783 .
Mosić, Mirjana D., Trifković, Jelena, Vovk, Irena, Gašić, Uroš M., Tešić, Živoslav Lj., Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen" in Biomolecules, 9, no. 12 (2019),
https://doi.org/10.3390/biom9120783 . .
1
36
14
37
25

Supplementary data for article: Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783

Mosić, Mirjana D.; Trifković, Jelena; Vovk, Irena; Gašić, Uroš M.; Tešić, Živoslav Lj.; Šikoparija, Branko; Milojković-Opsenica, Dušanka

(MDPI AG, 2019)

TY  - DATA
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3775
PB  - MDPI AG
T2  - Biomolecules
T1  - Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3775
ER  - 
@misc{
author = "Mosić, Mirjana D. and Trifković, Jelena and Vovk, Irena and Gašić, Uroš M. and Tešić, Živoslav Lj. and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3775"
}
Mosić, M. D., Trifković, J., Vovk, I., Gašić, U. M., Tešić, Ž. Lj., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783. in Biomolecules
MDPI AG..
https://hdl.handle.net/21.15107/rcub_cherry_3775
Mosić MD, Trifković J, Vovk I, Gašić UM, Tešić ŽL, Šikoparija B, Milojković-Opsenica D. Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783. in Biomolecules. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3775 .
Mosić, Mirjana D., Trifković, Jelena, Vovk, Irena, Gašić, Uroš M., Tešić, Živoslav Lj., Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783" in Biomolecules (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3775 .

Mineral content of bee pollen from Serbia

Kostić, Aleksandar Ž.; Pešić, Mirjana B.; Mosić, Mirjana D.; Dojčinović, Biljana P.; Natić, Maja; Trifković, Jelena

(Inst Medical Research & Occupational Health, Zagreb, 2015)

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Pešić, Mirjana B.
AU  - Mosić, Mirjana D.
AU  - Dojčinović, Biljana P.
AU  - Natić, Maja
AU  - Trifković, Jelena
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2051
AB  - In this study we analysed mineral composition of bee pollen of different plant origin collected across Serbia using inductively coupled plasma - optical emission spectrometry. The most abundant elements were potassium, calcium, and magnesium. The samples were also exceptionally rich in iron and zinc, which are very important as nutrients. Judging by our findings, mineral composition of bee pollen much more depends on the type of pollen-producing plant than on its geographical origin.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Mineral content of bee pollen from Serbia
VL  - 66
IS  - 4
SP  - 251
EP  - 258
DO  - 10.1515/aiht-2015-66-2630
ER  - 
@article{
author = "Kostić, Aleksandar Ž. and Pešić, Mirjana B. and Mosić, Mirjana D. and Dojčinović, Biljana P. and Natić, Maja and Trifković, Jelena",
year = "2015",
abstract = "In this study we analysed mineral composition of bee pollen of different plant origin collected across Serbia using inductively coupled plasma - optical emission spectrometry. The most abundant elements were potassium, calcium, and magnesium. The samples were also exceptionally rich in iron and zinc, which are very important as nutrients. Judging by our findings, mineral composition of bee pollen much more depends on the type of pollen-producing plant than on its geographical origin.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Mineral content of bee pollen from Serbia",
volume = "66",
number = "4",
pages = "251-258",
doi = "10.1515/aiht-2015-66-2630"
}
Kostić, A. Ž., Pešić, M. B., Mosić, M. D., Dojčinović, B. P., Natić, M.,& Trifković, J.. (2015). Mineral content of bee pollen from Serbia. in Arhiv za higijenu rada i toksikologiju
Inst Medical Research & Occupational Health, Zagreb., 66(4), 251-258.
https://doi.org/10.1515/aiht-2015-66-2630
Kostić AŽ, Pešić MB, Mosić MD, Dojčinović BP, Natić M, Trifković J. Mineral content of bee pollen from Serbia. in Arhiv za higijenu rada i toksikologiju. 2015;66(4):251-258.
doi:10.1515/aiht-2015-66-2630 .
Kostić, Aleksandar Ž., Pešić, Mirjana B., Mosić, Mirjana D., Dojčinović, Biljana P., Natić, Maja, Trifković, Jelena, "Mineral content of bee pollen from Serbia" in Arhiv za higijenu rada i toksikologiju, 66, no. 4 (2015):251-258,
https://doi.org/10.1515/aiht-2015-66-2630 . .
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