TY  - JOUR
AU  - Gojgić-Cvijović, Gordana D.
AU  - Milic, J. S.
AU  - Šolević-Knudsen, Tatjana
AU  - Beškoski, Vladimir
AU  - Ilić, Mila V.
AU  - Đokić, Lidija
AU  - Narancic, T. M.
AU  - Vrvić, Miroslav M.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1063
AB  - This article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. A bacterial consortium, consisting of strains from the genera Pseudomonas, Achromobacter, Bacillus and Micromonospora, was isolated from a petroleum sludge sample and characterized. The addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory conditions. The extent of biodegradation was monitored by the gravimetric method and analysis of the residual oil by gas chromatography. Over a 12-week experiment, the achieved degree of TPH (total petroleum hydrocarbon) degradation amounted to 82-88% in the petroleum sludge and 86-91% in the polluted soil. Gas chromatography-mass spectrometry was utilized to determine the biodegradability and degradation rates of n-alkanes, isoprenoids, steranes, diasteranes and terpanes. Complete degradation of the n-alkanes and isoprenoids fractions occurred in both the samples. In addition, the intensities of the peaks corresponding to tricyclic terpenes and homohopanes were decreased, while significant changes were also observed in the distribution of diasteranes and steranes.
PB  - Springer, New York
T2  - Biodegradation
T1  - Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study
VL  - 23
IS  - 1
SP  - 1
EP  - 14
DO  - 10.1007/s10532-011-9481-1
ER  - 

@article{
author = "Gojgić-Cvijović, Gordana D. and Milic, J. S. and Šolević-Knudsen, Tatjana and Beškoski, Vladimir and Ilić, Mila V. and Đokić, Lidija and Narancic, T. M. and Vrvić, Miroslav M.",
year = "2012",
abstract = "This article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. A bacterial consortium, consisting of strains from the genera Pseudomonas, Achromobacter, Bacillus and Micromonospora, was isolated from a petroleum sludge sample and characterized. The addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory conditions. The extent of biodegradation was monitored by the gravimetric method and analysis of the residual oil by gas chromatography. Over a 12-week experiment, the achieved degree of TPH (total petroleum hydrocarbon) degradation amounted to 82-88% in the petroleum sludge and 86-91% in the polluted soil. Gas chromatography-mass spectrometry was utilized to determine the biodegradability and degradation rates of n-alkanes, isoprenoids, steranes, diasteranes and terpanes. Complete degradation of the n-alkanes and isoprenoids fractions occurred in both the samples. In addition, the intensities of the peaks corresponding to tricyclic terpenes and homohopanes were decreased, while significant changes were also observed in the distribution of diasteranes and steranes.",
publisher = "Springer, New York",
journal = "Biodegradation",
title = "Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study",
volume = "23",
number = "1",
pages = "1-14",
doi = "10.1007/s10532-011-9481-1"
}

Gojgić-Cvijović, G. D., Milic, J. S., Šolević-Knudsen, T., Beškoski, V., Ilić, M. V., Đokić, L., Narancic, T. M.,& Vrvić, M. M.. (2012). Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. in Biodegradation
Springer, New York., 23(1), 1-14.
https://doi.org/10.1007/s10532-011-9481-1

Gojgić-Cvijović GD, Milic JS, Šolević-Knudsen T, Beškoski V, Ilić MV, Đokić L, Narancic TM, Vrvić MM. Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. in Biodegradation. 2012;23(1):1-14.
doi:10.1007/s10532-011-9481-1 .

Gojgić-Cvijović, Gordana D., Milic, J. S., Šolević-Knudsen, Tatjana, Beškoski, Vladimir, Ilić, Mila V., Đokić, Lidija, Narancic, T. M., Vrvić, Miroslav M., "Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study" in Biodegradation, 23, no. 1 (2012):1-14,
https://doi.org/10.1007/s10532-011-9481-1 . .

TY  - JOUR
AU  - Narancic, T.
AU  - Kenny, S. T.
AU  - Đokić, Lidija
AU  - Vasiljević, Branka
AU  - O'Connor, K. E.
AU  - Nikodinović-Runić, Jasmina
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1327
AB  - Aims The aim of this study was to convert numerous polyaromatic and monoaromatic hydrocarbons into biodegradable polymer medium-chain-length polyhydroxyalkanoate (mcl-PHA). Methods and Results Using naphthalene enrichment cultivation method, we have isolated seven bacterial strains from the river sediment exposed to petrochemical industry effluents. In addition to naphthalene, all seven strains could utilize between 12 and 17 different aromatic substrates, including toluene, benzene and biphenyl. Only one isolate that was identified as Pseudomonas sp. TN301 could accumulate mcl-PHA from naphthalene to 23% of cell dry weight. Owing to poor solubility, a method of supplying highly hydrophobic polyaromatic hydrocarbons to a culture medium was developed. The best biomass and mcl-PHA yields were achieved with the addition of synthetic surfactant Tween 80 (0.5 g l-1). We have shown that Pseudomonas sp. TN301 can accumulate mcl-PHA from a wide range of polyaromatic and monoaromatic hydrocarbons, and mixtures thereof, while it could also accumulate polyphosphates and was tolerant to the presence of heavy metal (100 mmol l-1 cadmium and 20 mmol l-1 nickel). Conclusions A new Pseudomonas strain was isolated and identified with the ability to accumulate mcl-PHA from a variety of aromatic hydrocarbons. Significance and Impact of the Study This study is the first report on the ability of a bacterial strain to convert a range of polyaromatic hydrocarbon compounds to the biodegradable polymer (mcl-PHA). Mcl-PHA is gaining importance as a promising biodegradable thermoelastomer, and therefore, isolation of new producing strains is highly significant. Furthermore, this strain has the ability to utilize a range of hydrocarbons, which often occur as mixtures and could potentially be employed in the recently described efforts to convert waste materials to PHA.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Applied Microbiology
T1  - Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons
VL  - 113
IS  - 3
SP  - 508
EP  - 520
DO  - 10.1111/j.1365-2672.2012.05353.x
ER  - 

@article{
author = "Narancic, T. and Kenny, S. T. and Đokić, Lidija and Vasiljević, Branka and O'Connor, K. E. and Nikodinović-Runić, Jasmina",
year = "2012",
abstract = "Aims The aim of this study was to convert numerous polyaromatic and monoaromatic hydrocarbons into biodegradable polymer medium-chain-length polyhydroxyalkanoate (mcl-PHA). Methods and Results Using naphthalene enrichment cultivation method, we have isolated seven bacterial strains from the river sediment exposed to petrochemical industry effluents. In addition to naphthalene, all seven strains could utilize between 12 and 17 different aromatic substrates, including toluene, benzene and biphenyl. Only one isolate that was identified as Pseudomonas sp. TN301 could accumulate mcl-PHA from naphthalene to 23% of cell dry weight. Owing to poor solubility, a method of supplying highly hydrophobic polyaromatic hydrocarbons to a culture medium was developed. The best biomass and mcl-PHA yields were achieved with the addition of synthetic surfactant Tween 80 (0.5 g l-1). We have shown that Pseudomonas sp. TN301 can accumulate mcl-PHA from a wide range of polyaromatic and monoaromatic hydrocarbons, and mixtures thereof, while it could also accumulate polyphosphates and was tolerant to the presence of heavy metal (100 mmol l-1 cadmium and 20 mmol l-1 nickel). Conclusions A new Pseudomonas strain was isolated and identified with the ability to accumulate mcl-PHA from a variety of aromatic hydrocarbons. Significance and Impact of the Study This study is the first report on the ability of a bacterial strain to convert a range of polyaromatic hydrocarbon compounds to the biodegradable polymer (mcl-PHA). Mcl-PHA is gaining importance as a promising biodegradable thermoelastomer, and therefore, isolation of new producing strains is highly significant. Furthermore, this strain has the ability to utilize a range of hydrocarbons, which often occur as mixtures and could potentially be employed in the recently described efforts to convert waste materials to PHA.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons",
volume = "113",
number = "3",
pages = "508-520",
doi = "10.1111/j.1365-2672.2012.05353.x"
}

Narancic, T., Kenny, S. T., Đokić, L., Vasiljević, B., O'Connor, K. E.,& Nikodinović-Runić, J.. (2012). Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons. in Journal of Applied Microbiology
Wiley-Blackwell, Hoboken., 113(3), 508-520.
https://doi.org/10.1111/j.1365-2672.2012.05353.x

Narancic T, Kenny ST, Đokić L, Vasiljević B, O'Connor KE, Nikodinović-Runić J. Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons. in Journal of Applied Microbiology. 2012;113(3):508-520.
doi:10.1111/j.1365-2672.2012.05353.x .

Narancic, T., Kenny, S. T., Đokić, Lidija, Vasiljević, Branka, O'Connor, K. E., Nikodinović-Runić, Jasmina, "Medium-chain-length polyhydroxyalkanoate production by newly isolated Pseudomonas sp TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons" in Journal of Applied Microbiology, 113, no. 3 (2012):508-520,
https://doi.org/10.1111/j.1365-2672.2012.05353.x . .