TY  - JOUR
AU  - Polović, Natalija
AU  - Obradovic, A.
AU  - Spasic, M.
AU  - Plecas-Solarovic, B.
AU  - Gavrović-Jankulović, Marija
AU  - Ćirković-Veličković, Tanja
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/108
AB  - Food allergens must exhibit sufficient gastrointestinal stability to reach the intestinal mucosa where absorption and sensitization can occur. Therefore, investigation of protein stability within the gastrointestinal tract may provide a prospective test for the allergenic potential of novel proteins. The aim of this work was to examine the effect of the fruit matrix and purified pectin on the digestion in vivo of kiwifruit allergens in the rat gastrointestinal system. The major kiwi allergen, Act d 2, was quantified in several compartments of the gastrointestinal tract by a monoclonal antibody-based ELISA. Protein intactness was demonstrated by immunoblot. Under conditions of complex food digestion in vivo, a pepsin-labile protein survived passage from the stomach to the caecum during a 3-h period. Decay of Act d 2 in the rat gut exhibited an exponential pattern. Ingestion of kiwifruit was followed by a decrease in both total and specific pepsin activity. When purified, Act d 2 allergen was consumed together with pure apple pectin; both gastric acidity as well as specific and total pepsin activity declined and thus protected 23% of the ingested allergen from digestion for 90 min. In conclusion, ingestion of pectin-rich fruits and particularly pectin supplements may have a protective action on pepsin-labile allergens and prolong their survival in both gastric and duodenal juices, enabling efficient uptake and presentation to the immune system. © Springer Science+Business Media, LLC 2010.
T2  - Food Digestion
T1  - In vivo digestion of a thaumatin-like kiwifruit protein in rats
VL  - 1
IS  - 1-2
SP  - 5
EP  - 13
DO  - 10.1007/s13228-010-0001-2
ER  - 

@article{
author = "Polović, Natalija and Obradovic, A. and Spasic, M. and Plecas-Solarovic, B. and Gavrović-Jankulović, Marija and Ćirković-Veličković, Tanja",
year = "2010",
abstract = "Food allergens must exhibit sufficient gastrointestinal stability to reach the intestinal mucosa where absorption and sensitization can occur. Therefore, investigation of protein stability within the gastrointestinal tract may provide a prospective test for the allergenic potential of novel proteins. The aim of this work was to examine the effect of the fruit matrix and purified pectin on the digestion in vivo of kiwifruit allergens in the rat gastrointestinal system. The major kiwi allergen, Act d 2, was quantified in several compartments of the gastrointestinal tract by a monoclonal antibody-based ELISA. Protein intactness was demonstrated by immunoblot. Under conditions of complex food digestion in vivo, a pepsin-labile protein survived passage from the stomach to the caecum during a 3-h period. Decay of Act d 2 in the rat gut exhibited an exponential pattern. Ingestion of kiwifruit was followed by a decrease in both total and specific pepsin activity. When purified, Act d 2 allergen was consumed together with pure apple pectin; both gastric acidity as well as specific and total pepsin activity declined and thus protected 23% of the ingested allergen from digestion for 90 min. In conclusion, ingestion of pectin-rich fruits and particularly pectin supplements may have a protective action on pepsin-labile allergens and prolong their survival in both gastric and duodenal juices, enabling efficient uptake and presentation to the immune system. © Springer Science+Business Media, LLC 2010.",
journal = "Food Digestion",
title = "In vivo digestion of a thaumatin-like kiwifruit protein in rats",
volume = "1",
number = "1-2",
pages = "5-13",
doi = "10.1007/s13228-010-0001-2"
}

Polović, N., Obradovic, A., Spasic, M., Plecas-Solarovic, B., Gavrović-Jankulović, M.,& Ćirković-Veličković, T.. (2010). In vivo digestion of a thaumatin-like kiwifruit protein in rats. in Food Digestion, 1(1-2), 5-13.
https://doi.org/10.1007/s13228-010-0001-2

Polović N, Obradovic A, Spasic M, Plecas-Solarovic B, Gavrović-Jankulović M, Ćirković-Veličković T. In vivo digestion of a thaumatin-like kiwifruit protein in rats. in Food Digestion. 2010;1(1-2):5-13.
doi:10.1007/s13228-010-0001-2 .

Polović, Natalija, Obradovic, A., Spasic, M., Plecas-Solarovic, B., Gavrović-Jankulović, Marija, Ćirković-Veličković, Tanja, "In vivo digestion of a thaumatin-like kiwifruit protein in rats" in Food Digestion, 1, no. 1-2 (2010):5-13,
https://doi.org/10.1007/s13228-010-0001-2 . .

TY  - CONF
AU  - Polović, Natalija
AU  - Obradovic, A.
AU  - Spasic, M.
AU  - Plecas, B.
AU  - Burazer, Lidija M.
AU  - Gavrović-Jankulović, Marija
AU  - Jankov, Ratko M.
AU  - Ćirković-Veličković, Tanja
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/938
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - Transit times of kiwi fruit proteins through rat's gastrointestinal
VL  - 63
SP  - 428
EP  - 429
UR  - https://hdl.handle.net/21.15107/rcub_cherry_938
ER  - 

@conference{
author = "Polović, Natalija and Obradovic, A. and Spasic, M. and Plecas, B. and Burazer, Lidija M. and Gavrović-Jankulović, Marija and Jankov, Ratko M. and Ćirković-Veličković, Tanja",
year = "2008",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "Transit times of kiwi fruit proteins through rat's gastrointestinal",
volume = "63",
pages = "428-429",
url = "https://hdl.handle.net/21.15107/rcub_cherry_938"
}

Polović, N., Obradovic, A., Spasic, M., Plecas, B., Burazer, L. M., Gavrović-Jankulović, M., Jankov, R. M.,& Ćirković-Veličković, T.. (2008). Transit times of kiwi fruit proteins through rat's gastrointestinal. in Allergy
Blackwell Publishing, Oxford., 63, 428-429.
https://hdl.handle.net/21.15107/rcub_cherry_938

Polović N, Obradovic A, Spasic M, Plecas B, Burazer LM, Gavrović-Jankulović M, Jankov RM, Ćirković-Veličković T. Transit times of kiwi fruit proteins through rat's gastrointestinal. in Allergy. 2008;63:428-429.
https://hdl.handle.net/21.15107/rcub_cherry_938 .

Polović, Natalija, Obradovic, A., Spasic, M., Plecas, B., Burazer, Lidija M., Gavrović-Jankulović, Marija, Jankov, Ratko M., Ćirković-Veličković, Tanja, "Transit times of kiwi fruit proteins through rat's gastrointestinal" in Allergy, 63 (2008):428-429,
https://hdl.handle.net/21.15107/rcub_cherry_938 .

TY  - CONF
AU  - Gavrović-Jankulović, Marija
AU  - Spasic, M.
AU  - Ćirković-Veličković, Tanja
AU  - Atanasković-Marković, Marina
AU  - Lindner, B.
AU  - Petersen, A.
AU  - Becker, W.
AU  - Jankov, Ratko M.
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/833
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - Production of a recombinant thaumatin-like kiwi allergen act c 2
VL  - 62
SP  - 341
EP  - 342
UR  - https://hdl.handle.net/21.15107/rcub_cherry_833
ER  - 

@conference{
author = "Gavrović-Jankulović, Marija and Spasic, M. and Ćirković-Veličković, Tanja and Atanasković-Marković, Marina and Lindner, B. and Petersen, A. and Becker, W. and Jankov, Ratko M.",
year = "2007",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "Production of a recombinant thaumatin-like kiwi allergen act c 2",
volume = "62",
pages = "341-342",
url = "https://hdl.handle.net/21.15107/rcub_cherry_833"
}

Gavrović-Jankulović, M., Spasic, M., Ćirković-Veličković, T., Atanasković-Marković, M., Lindner, B., Petersen, A., Becker, W.,& Jankov, R. M.. (2007). Production of a recombinant thaumatin-like kiwi allergen act c 2. in Allergy
Blackwell Publishing, Oxford., 62, 341-342.
https://hdl.handle.net/21.15107/rcub_cherry_833

Gavrović-Jankulović M, Spasic M, Ćirković-Veličković T, Atanasković-Marković M, Lindner B, Petersen A, Becker W, Jankov RM. Production of a recombinant thaumatin-like kiwi allergen act c 2. in Allergy. 2007;62:341-342.
https://hdl.handle.net/21.15107/rcub_cherry_833 .

Gavrović-Jankulović, Marija, Spasic, M., Ćirković-Veličković, Tanja, Atanasković-Marković, Marina, Lindner, B., Petersen, A., Becker, W., Jankov, Ratko M., "Production of a recombinant thaumatin-like kiwi allergen act c 2" in Allergy, 62 (2007):341-342,
https://hdl.handle.net/21.15107/rcub_cherry_833 .

TY  - CONF
AU  - Spasic, M.
AU  - Gavrović-Jankulović, Marija
AU  - Becker, W.
AU  - Petersen, A.
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/766
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - The development of an enzyme test for detection of Phl p 13 polygalacturonase activity
VL  - 62
SP  - 182
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_cherry_766
ER  - 

@conference{
author = "Spasic, M. and Gavrović-Jankulović, Marija and Becker, W. and Petersen, A.",
year = "2007",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "The development of an enzyme test for detection of Phl p 13 polygalacturonase activity",
volume = "62",
pages = "182-183",
url = "https://hdl.handle.net/21.15107/rcub_cherry_766"
}

Spasic, M., Gavrović-Jankulović, M., Becker, W.,& Petersen, A.. (2007). The development of an enzyme test for detection of Phl p 13 polygalacturonase activity. in Allergy
Blackwell Publishing, Oxford., 62, 182-183.
https://hdl.handle.net/21.15107/rcub_cherry_766

Spasic M, Gavrović-Jankulović M, Becker W, Petersen A. The development of an enzyme test for detection of Phl p 13 polygalacturonase activity. in Allergy. 2007;62:182-183.
https://hdl.handle.net/21.15107/rcub_cherry_766 .

Spasic, M., Gavrović-Jankulović, Marija, Becker, W., Petersen, A., "The development of an enzyme test for detection of Phl p 13 polygalacturonase activity" in Allergy, 62 (2007):182-183,
https://hdl.handle.net/21.15107/rcub_cherry_766 .