@conference{
author = "Ivković, Đurđa and Lazović, Mila and Jankov, Milica and Ristivojević, Petar",
year = "2022",
abstract = "Propolis is a resinous substance collected by honeybees (Apis mellifera L.) from
different plant parts such as branches, plant buds, leaves, and exudates partially digested
by β-glycosidase from bees’ saliva, and after that mixed with beeswax. It is commonly
used in folk medicine to prevent and treat colds, ulcers, rheumatism, diabetes, and dental
caries. Commercially available products most often comprise ethanolic extracts of
propolis. However, high alcohol content and strong residual flavour make such products
unsuitable for patients intolerant to alcohol, especially children. Due to mentioned
drawbacks, propylene glycolic, aqueous, fructose, or olive oil propolis extracts are
commonly found on the market as safer alternatives. These solvents are more appealing
options but the extraction efficiency is up to 10 times lower compared to ethanol. Thus,
there are high demands for the development of effective and ethanol-free extractions1,2
.
The overall aim of the study is to determine total phenolic, flavonoid content and radical
scavenging activity of high-quality and natural-based propolis extract enriched with
bioactives using new generation of solvents as Natural Deep Eutectic Solvents
(NADES)1
. Thirty-eight different green NADES have been used for the extraction of
phenols from poplar types of propolis. The NADES were prepared by mixing different
molar ratios of polycarboxylic acids, amino acids, glycerol, urea, potassium acetate,
choline chloride and ethylene glycol. The highest values of TPC, TFC, and DPPH were
obtained using following NADES systems: lactic acid/glycerol=1/1; lactic
acid/glycine=3/1; glycerol/proline=1/1; choline chloride/succinic acid=2/1 and choline
chloride/ethylene glycol=1/4.
References
1. V. Bankova, B. Trusheva & Milena Popova, J. Apic. Res. 2021, 60, 734.
2. L. Kubiliene, V. Laugaliene, A. Pavilonis, A. Maruska, D. Majiene, K. Barcauskaite,
BMC Complement. Altern. Med. 2015, 15, 1.
Acknowledgment
This work was supported by the Ministry of Education, Science and Technological
Development of the Republic of Serbia, Contract numbers: 451-03-68/2022-14/200168
and 451-03-68/2022-14/200288.",
journal = "8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts",
title = "Total phenolic, flavonoid contents and radical scavenging activities of NADES- based propolis extracts",
number = "35",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6223"
}