TY  - JOUR
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Šenerović, Lidija
AU  - Krivokapic, Nevena
AU  - Mitrovic, Tatjana
AU  - Bozovic, Nikola
AU  - Vasic, Vesna
AU  - Nikodinović-Runić, Jasmina
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2373
AB  - It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((5'2)-4'-methoxy-5'-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5'H-2,2'-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Experimental and Toxicologic Pathology
T1  - Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro
VL  - 69
IS  - 1
SP  - 55
EP  - 62
DO  - 10.1016/j.etp.2016.11.003
ER  - 

@article{
author = "Lazovic, Sasa and Leskovac, Andreja and Petrović, Sandra and Šenerović, Lidija and Krivokapic, Nevena and Mitrovic, Tatjana and Bozovic, Nikola and Vasic, Vesna and Nikodinović-Runić, Jasmina",
year = "2017",
abstract = "It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((5'2)-4'-methoxy-5'-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5'H-2,2'-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Experimental and Toxicologic Pathology",
title = "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro",
volume = "69",
number = "1",
pages = "55-62",
doi = "10.1016/j.etp.2016.11.003"
}

Lazovic, S., Leskovac, A., Petrović, S., Šenerović, L., Krivokapic, N., Mitrovic, T., Bozovic, N., Vasic, V.,& Nikodinović-Runić, J.. (2017). Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 69(1), 55-62.
https://doi.org/10.1016/j.etp.2016.11.003

Lazovic S, Leskovac A, Petrović S, Šenerović L, Krivokapic N, Mitrovic T, Bozovic N, Vasic V, Nikodinović-Runić J. Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology. 2017;69(1):55-62.
doi:10.1016/j.etp.2016.11.003 .

Lazovic, Sasa, Leskovac, Andreja, Petrović, Sandra, Šenerović, Lidija, Krivokapic, Nevena, Mitrovic, Tatjana, Bozovic, Nikola, Vasic, Vesna, Nikodinović-Runić, Jasmina, "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro" in Experimental and Toxicologic Pathology, 69, no. 1 (2017):55-62,
https://doi.org/10.1016/j.etp.2016.11.003 . .

TY  - JOUR
AU  - Pasti, Tamara Lazarevic
AU  - Momic, Tatjana
AU  - Onjia, Antonije E.
AU  - Vujisić, Ljubodrag V.
AU  - Vasic, Vesna
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1106
AB  - In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
PB  - Springer Wien, Wien
T2  - Microchimica Acta
T1  - Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods
VL  - 170
IS  - 3-4
SP  - 289
EP  - 297
DO  - 10.1007/s00604-010-0324-2
ER  - 

@article{
author = "Pasti, Tamara Lazarevic and Momic, Tatjana and Onjia, Antonije E. and Vujisić, Ljubodrag V. and Vasic, Vesna",
year = "2010",
abstract = "In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.",
publisher = "Springer Wien, Wien",
journal = "Microchimica Acta",
title = "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods",
volume = "170",
number = "3-4",
pages = "289-297",
doi = "10.1007/s00604-010-0324-2"
}

Pasti, T. L., Momic, T., Onjia, A. E., Vujisić, L. V.,& Vasic, V.. (2010). Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta
Springer Wien, Wien., 170(3-4), 289-297.
https://doi.org/10.1007/s00604-010-0324-2

Pasti TL, Momic T, Onjia AE, Vujisić LV, Vasic V. Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta. 2010;170(3-4):289-297.
doi:10.1007/s00604-010-0324-2 .

Pasti, Tamara Lazarevic, Momic, Tatjana, Onjia, Antonije E., Vujisić, Ljubodrag V., Vasic, Vesna, "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods" in Microchimica Acta, 170, no. 3-4 (2010):289-297,
https://doi.org/10.1007/s00604-010-0324-2 . .

TY  - JOUR
AU  - Momic, Tatjana
AU  - Vujčić, Zoran
AU  - Vasic, Vesna
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/946
AB  - The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc.
PB  - Wiley-Blackwell, Malden
T2  - International Journal of Chemical Kinetics
T1  - Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin
VL  - 40
IS  - 7
SP  - 384
EP  - 394
DO  - 10.1002/kin.20319
ER  - 

@article{
author = "Momic, Tatjana and Vujčić, Zoran and Vasic, Vesna",
year = "2008",
abstract = "The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc.",
publisher = "Wiley-Blackwell, Malden",
journal = "International Journal of Chemical Kinetics",
title = "Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin",
volume = "40",
number = "7",
pages = "384-394",
doi = "10.1002/kin.20319"
}

Momic, T., Vujčić, Z.,& Vasic, V.. (2008). Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin. in International Journal of Chemical Kinetics
Wiley-Blackwell, Malden., 40(7), 384-394.
https://doi.org/10.1002/kin.20319

Momic T, Vujčić Z, Vasic V. Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin. in International Journal of Chemical Kinetics. 2008;40(7):384-394.
doi:10.1002/kin.20319 .

Momic, Tatjana, Vujčić, Zoran, Vasic, Vesna, "Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin" in International Journal of Chemical Kinetics, 40, no. 7 (2008):384-394,
https://doi.org/10.1002/kin.20319 . .

TY  - JOUR
AU  - Momic, T
AU  - Vujčić, Zoran
AU  - Vasic, V
AU  - Horvat, A
PY  - 2002
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/530
AB  - Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.
AB  - Delimično prečišćena Na,K-ATPaza sinaptičkih plazma–membrana (SPM) mozga pacova imobilizovana je adsorpcijom na nitrocelulozne (NC) poliviniliden-fluorid (PVDF) membrane i membrane od staklenih vlakana (SV). Aktivnost enzima delimično prečišćenog solubilizacijom SDS-om povećana je oko 40%. Najveći procenat aktivnosti (14%) enzim zadržava posle 30 minuta adsorpcije SPM na 20ºC, pod vakuumom, sa 25 μg proteina po nitroceluloznom disku. Na,K-ATPaza imobilizovana na nitroceluloznoj membrani stabilna je 72 sata na 20ºC. Adsorpcijom, osetljivost enzima na inhibiciju Hg2+ i Cd2+ se povećava. Rezultati pokazuju da se imobilizovana Na,K-ATPaza SPM može koristiti za detekciju toksičnih metalnih jona u različitim uzorcima.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes
T1  - Imobilizacija Na,K-ATPpaze izolovane iz sinaptičkih plazma-membrana mozga pacova
VL  - 67
IS  - 12
SP  - 809
EP  - 817
DO  - 10.2298/JSC0212809M
ER  - 

@article{
author = "Momic, T and Vujčić, Zoran and Vasic, V and Horvat, A",
year = "2002",
abstract = "Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples., Delimično prečišćena Na,K-ATPaza sinaptičkih plazma–membrana (SPM) mozga pacova imobilizovana je adsorpcijom na nitrocelulozne (NC) poliviniliden-fluorid (PVDF) membrane i membrane od staklenih vlakana (SV). Aktivnost enzima delimično prečišćenog solubilizacijom SDS-om povećana je oko 40%. Najveći procenat aktivnosti (14%) enzim zadržava posle 30 minuta adsorpcije SPM na 20ºC, pod vakuumom, sa 25 μg proteina po nitroceluloznom disku. Na,K-ATPaza imobilizovana na nitroceluloznoj membrani stabilna je 72 sata na 20ºC. Adsorpcijom, osetljivost enzima na inhibiciju Hg2+ i Cd2+ se povećava. Rezultati pokazuju da se imobilizovana Na,K-ATPaza SPM može koristiti za detekciju toksičnih metalnih jona u različitim uzorcima.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes, Imobilizacija Na,K-ATPpaze izolovane iz sinaptičkih plazma-membrana mozga pacova",
volume = "67",
number = "12",
pages = "809-817",
doi = "10.2298/JSC0212809M"
}

Momic, T., Vujčić, Z., Vasic, V.,& Horvat, A.. (2002). Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 67(12), 809-817.
https://doi.org/10.2298/JSC0212809M

Momic T, Vujčić Z, Vasic V, Horvat A. Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society. 2002;67(12):809-817.
doi:10.2298/JSC0212809M .

Momic, T, Vujčić, Zoran, Vasic, V, Horvat, A, "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes" in Journal of the Serbian Chemical Society, 67, no. 12 (2002):809-817,
https://doi.org/10.2298/JSC0212809M . .