TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Mišić, Milan
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5796
AB  - Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.
T2  - Fuel
T1  - Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch
VL  - 338
SP  - 127363
DO  - 10.1016/j.fuel.2022.127363
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Mišić, Milan and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.",
journal = "Fuel",
title = "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch",
volume = "338",
pages = "127363",
doi = "10.1016/j.fuel.2022.127363"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363.
https://doi.org/10.1016/j.fuel.2022.127363

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Mišić M, Božić N, Vujčić Z. Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel. 2023;338:127363.
doi:10.1016/j.fuel.2022.127363 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Mišić, Milan, Božić, Nataša, Vujčić, Zoran, "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch" in Fuel, 338 (2023):127363,
https://doi.org/10.1016/j.fuel.2022.127363 . .

TY  - DATA
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Mišić, Milan
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5836
AB  - Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.
T2  - Fuel
T1  - Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363
VL  - 338
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5836
ER  - 

@misc{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Mišić, Milan and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.",
journal = "Fuel",
title = "Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363",
volume = "338",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5836"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363. in Fuel, 338.
https://hdl.handle.net/21.15107/rcub_cherry_5836

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Mišić M, Božić N, Vujčić Z. Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363. in Fuel. 2023;338.
https://hdl.handle.net/21.15107/rcub_cherry_5836 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Mišić, Milan, Božić, Nataša, Vujčić, Zoran, "Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363" in Fuel, 338 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5836 .

TY  - THES
AU  - Mišić, Milan
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4139
AB  - Mnogi aktivni sastojci savremenih medicinskih sredstava su u originalu pronađeni, ili izolovani iz biljaka. Milenijumima su lekovite biljke bile korišćene u kulinarstvu kao začini, za dekoraciju, tretman mnogih bolesti, kao i u svrhu konzervisanja namirnica. Razvojem savremene biohemije, medicine i farmaceutske tehnologije, upotreba biljaka u lečenju se zasniva na upotrebi
pojedinih izolovanih komponenti iz biljaka. Upotreba biljaka, njihovih ekstrakata, čajeva i tinktura obuhvata tradicionalnu medicinu ili u novije vreme alternativnu medicinu. Iz lekovitih biljaka komponente koje se koriste i izoluju su etarska ulja (i pojedinačne komponente iz njih), alkaloidi, polifenoli i druge komponente koje se nalaze u različitim oblicima. Polifenoli su sekundarni metaboliti biljaka, čija je verovatna uloga zaštitna od predatora i patogena. Komponente ovih biljaka se nalaze u prirodi i ne znači automatski da su uvek zdrave i korisne. Naprotiv, nekada komponente biljaka mogu biti toksične ili količina komponenti koje nisu
toksične može preći bezbedne količine u korišćenim preparatima. Ovo treba imati u vidu, pogotovu sa današnjom težnjom da se sintetičke komponente, u farmaceutskoj industriji, zamene prirodnim. Zbog ovoga je važno ispitivanje karakteristika pojedinačnih komponenti, izolovanih iz određenih biljaka.
Tema ovog rada su polifenoli biljaka i njihove osobine, antioksidativnost i ispitivanje interakcija polifenola i proteina. Polifenoli su ekstrahovani iz nane, rtanjskog čaja, žalfije i kamilice. Ispitane su antioksidativna aktivnost, interakcije sa albuminom iz goveđeg seruma i proteinima surutke. Mnogo radova je fokusirano na interakcije prirodnih polifenola sa proteinima u ustima i njihov uticaj na ukus ili na uticaj čistih hemijski sintetisanih polifenola na proteine. Zbog toga su u ovom radu ispitane interakcije svežih ekstrakata biljaka (čajeva i koncentrovanijih ekstrakata) na proteine.
Cilj ovog rada je bio ispitivanje antioksidativnosti rtanjskog čaja, nane, žalfije i kamilice i ispitivanje interakcija polifenola iz ekstrakata ovih biljaka sa albuminom iz goveđeg seruma i proteinima iz surutke.
T1  - Ispitivanje antioksidativnog potencijala i interakcija domaćih čajeva i albumina spektrometrijskim i elektroforetskim metodama
SP  - 1
EP  - 59
EP  - 
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4139
ER  - 

@mastersthesis{
author = "Mišić, Milan",
year = "2020",
abstract = "Mnogi aktivni sastojci savremenih medicinskih sredstava su u originalu pronađeni, ili izolovani iz biljaka. Milenijumima su lekovite biljke bile korišćene u kulinarstvu kao začini, za dekoraciju, tretman mnogih bolesti, kao i u svrhu konzervisanja namirnica. Razvojem savremene biohemije, medicine i farmaceutske tehnologije, upotreba biljaka u lečenju se zasniva na upotrebi
pojedinih izolovanih komponenti iz biljaka. Upotreba biljaka, njihovih ekstrakata, čajeva i tinktura obuhvata tradicionalnu medicinu ili u novije vreme alternativnu medicinu. Iz lekovitih biljaka komponente koje se koriste i izoluju su etarska ulja (i pojedinačne komponente iz njih), alkaloidi, polifenoli i druge komponente koje se nalaze u različitim oblicima. Polifenoli su sekundarni metaboliti biljaka, čija je verovatna uloga zaštitna od predatora i patogena. Komponente ovih biljaka se nalaze u prirodi i ne znači automatski da su uvek zdrave i korisne. Naprotiv, nekada komponente biljaka mogu biti toksične ili količina komponenti koje nisu
toksične može preći bezbedne količine u korišćenim preparatima. Ovo treba imati u vidu, pogotovu sa današnjom težnjom da se sintetičke komponente, u farmaceutskoj industriji, zamene prirodnim. Zbog ovoga je važno ispitivanje karakteristika pojedinačnih komponenti, izolovanih iz određenih biljaka.
Tema ovog rada su polifenoli biljaka i njihove osobine, antioksidativnost i ispitivanje interakcija polifenola i proteina. Polifenoli su ekstrahovani iz nane, rtanjskog čaja, žalfije i kamilice. Ispitane su antioksidativna aktivnost, interakcije sa albuminom iz goveđeg seruma i proteinima surutke. Mnogo radova je fokusirano na interakcije prirodnih polifenola sa proteinima u ustima i njihov uticaj na ukus ili na uticaj čistih hemijski sintetisanih polifenola na proteine. Zbog toga su u ovom radu ispitane interakcije svežih ekstrakata biljaka (čajeva i koncentrovanijih ekstrakata) na proteine.
Cilj ovog rada je bio ispitivanje antioksidativnosti rtanjskog čaja, nane, žalfije i kamilice i ispitivanje interakcija polifenola iz ekstrakata ovih biljaka sa albuminom iz goveđeg seruma i proteinima iz surutke.",
title = "Ispitivanje antioksidativnog potencijala i interakcija domaćih čajeva i albumina spektrometrijskim i elektroforetskim metodama",
pages = "1-59-",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4139"
}

Mišić, M.. (2020). Ispitivanje antioksidativnog potencijala i interakcija domaćih čajeva i albumina spektrometrijskim i elektroforetskim metodama. , 1-59.
https://hdl.handle.net/21.15107/rcub_cherry_4139

Mišić M. Ispitivanje antioksidativnog potencijala i interakcija domaćih čajeva i albumina spektrometrijskim i elektroforetskim metodama. 2020;:1-59.
https://hdl.handle.net/21.15107/rcub_cherry_4139 .

Mišić, Milan, "Ispitivanje antioksidativnog potencijala i interakcija domaćih čajeva i albumina spektrometrijskim i elektroforetskim metodama" (2020):1-59,
https://hdl.handle.net/21.15107/rcub_cherry_4139 .