TY  - JOUR
AU  - Zelenović, Nevena D.
AU  - Kojadinović, Milica I.
AU  - Filipović, Lidija
AU  - Vučić, Vesna M.
AU  - Milčić, Miloš K.
AU  - Arsic, Aleksndra
AU  - Popović, Milica M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6257
AB  - Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.
PB  - SAGE Publications
T2  - Natural Product Communications
T1  - Interactions of Different Urolithins With Bovine Serum Albumin
VL  - 18
IS  - 5
DO  - 10.1177/1934578X231169366
ER  - 

@article{
author = "Zelenović, Nevena D. and Kojadinović, Milica I. and Filipović, Lidija and Vučić, Vesna M. and Milčić, Miloš K. and Arsic, Aleksndra and Popović, Milica M.",
year = "2023",
abstract = "Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.",
publisher = "SAGE Publications",
journal = "Natural Product Communications",
title = "Interactions of Different Urolithins With Bovine Serum Albumin",
volume = "18",
number = "5",
doi = "10.1177/1934578X231169366"
}

Zelenović, N. D., Kojadinović, M. I., Filipović, L., Vučić, V. M., Milčić, M. K., Arsic, A.,& Popović, M. M.. (2023). Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications
SAGE Publications., 18(5).
https://doi.org/10.1177/1934578X231169366

Zelenović ND, Kojadinović MI, Filipović L, Vučić VM, Milčić MK, Arsic A, Popović MM. Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications. 2023;18(5).
doi:10.1177/1934578X231169366 .

Zelenović, Nevena D., Kojadinović, Milica I., Filipović, Lidija, Vučić, Vesna M., Milčić, Miloš K., Arsic, Aleksndra, Popović, Milica M., "Interactions of Different Urolithins With Bovine Serum Albumin" in Natural Product Communications, 18, no. 5 (2023),
https://doi.org/10.1177/1934578X231169366 . .

TY  - JOUR
AU  - FIlipović, Lidija
AU  - Kojadinović, Milica I.
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4989
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?
VL  - 68
SP  - 103057
DO  - 10.1016/j.jddst.2021.103057
ER  - 

@article{
author = "FIlipović, Lidija and Kojadinović, Milica I. and Popović, Milica M.",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?",
volume = "68",
pages = "103057",
doi = "10.1016/j.jddst.2021.103057"
}

FIlipović, L., Kojadinović, M. I.,& Popović, M. M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057

FIlipović L, Kojadinović MI, Popović MM. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .

FIlipović, Lidija, Kojadinović, Milica I., Popović, Milica M., "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

TY  - JOUR
AU  - FIlipović, Lidija
AU  - Kojadinović, Milica I.
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4997
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?
VL  - 68
SP  - 103057
DO  - 10.1016/j.jddst.2021.103057
ER  - 

@article{
author = "FIlipović, Lidija and Kojadinović, Milica I. and Popović, Milica M.",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?",
volume = "68",
pages = "103057",
doi = "10.1016/j.jddst.2021.103057"
}

FIlipović, L., Kojadinović, M. I.,& Popović, M. M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057

FIlipović L, Kojadinović MI, Popović MM. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .

FIlipović, Lidija, Kojadinović, Milica I., Popović, Milica M., "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

TY  - JOUR
AU  - Kojadinović, Milica I.
AU  - Glibetić, Marija D.
AU  - Vučić, Vesna M.
AU  - Popović, Milica M.
AU  - Vidović, Nevena Đ.
AU  - Debeljak-Martačić, Jasmina D.
AU  - Arsić, Aleksandra Č.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4791
AB  - Pomegranate juice (PJ) has potential positive effects in patients with metabolic disturbances due to a high content of polyphenols. The objective of this study was to evaluate effects of a 2-week consumption of dietary doses of PJ on blood pressure, lipid metabolism, and oxidative stress markers in overweight patients with dyslipidemia. Twenty-four patients, 8 males and 16 females, 40-60 years of age, with established overweight and dyslipidemia were randomly assigned into intervention group, who consumed 300 mL of PJ daily for 2 weeks, or control group. After 2 weeks of juice intake, intervention group had significantly lower diastolic blood pressure, low-density lipoprotein cholesterol, aminotransferase, and activity of glutathione peroxidase. Furthermore, patients who consumed PJ had reduced percentage of docosahexaenoic acid (22:6n-3, DHA) in plasma phospholipids and increased estimated activity of stearoyl-CoA desaturase. In erythrocytes, we found a significant increase in the levels of dihomo-gamma- linolenic acid (20:3n-6, DGLA) and DHA, as well as in estimated activity of Delta 6 desaturase, and a decrease in estimated activity of Delta 5 desaturase. These results show that even a short-term consumption of dietary doses of PJ exerts beneficial effects and affects lipid metabolism in overweight patients with dyslipidemia.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia
VL  - 24
IS  - 9
DO  - 10.1089/jmf.2020.0122
ER  - 

@article{
author = "Kojadinović, Milica I. and Glibetić, Marija D. and Vučić, Vesna M. and Popović, Milica M. and Vidović, Nevena Đ. and Debeljak-Martačić, Jasmina D. and Arsić, Aleksandra Č.",
year = "2021",
abstract = "Pomegranate juice (PJ) has potential positive effects in patients with metabolic disturbances due to a high content of polyphenols. The objective of this study was to evaluate effects of a 2-week consumption of dietary doses of PJ on blood pressure, lipid metabolism, and oxidative stress markers in overweight patients with dyslipidemia. Twenty-four patients, 8 males and 16 females, 40-60 years of age, with established overweight and dyslipidemia were randomly assigned into intervention group, who consumed 300 mL of PJ daily for 2 weeks, or control group. After 2 weeks of juice intake, intervention group had significantly lower diastolic blood pressure, low-density lipoprotein cholesterol, aminotransferase, and activity of glutathione peroxidase. Furthermore, patients who consumed PJ had reduced percentage of docosahexaenoic acid (22:6n-3, DHA) in plasma phospholipids and increased estimated activity of stearoyl-CoA desaturase. In erythrocytes, we found a significant increase in the levels of dihomo-gamma- linolenic acid (20:3n-6, DGLA) and DHA, as well as in estimated activity of Delta 6 desaturase, and a decrease in estimated activity of Delta 5 desaturase. These results show that even a short-term consumption of dietary doses of PJ exerts beneficial effects and affects lipid metabolism in overweight patients with dyslipidemia.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia",
volume = "24",
number = "9",
doi = "10.1089/jmf.2020.0122"
}

Kojadinović, M. I., Glibetić, M. D., Vučić, V. M., Popović, M. M., Vidović, N. Đ., Debeljak-Martačić, J. D.,& Arsić, A. Č.. (2021). Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 24(9).
https://doi.org/10.1089/jmf.2020.0122

Kojadinović MI, Glibetić MD, Vučić VM, Popović MM, Vidović NĐ, Debeljak-Martačić JD, Arsić AČ. Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia. in Journal of Medicinal Food. 2021;24(9).
doi:10.1089/jmf.2020.0122 .

Kojadinović, Milica I., Glibetić, Marija D., Vučić, Vesna M., Popović, Milica M., Vidović, Nevena Đ., Debeljak-Martačić, Jasmina D., Arsić, Aleksandra Č., "Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia" in Journal of Medicinal Food, 24, no. 9 (2021),
https://doi.org/10.1089/jmf.2020.0122 . .

TY  - JOUR
AU  - Kojadinović, Milica I.
AU  - Arsic, Aleksandra
AU  - Petovic-Oggiano, Gordana
AU  - Gavrović-Jankulović, Marija
AU  - Glibetic, Marija
AU  - Popović, Milica M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2551
AB  - Urolithins (UROs) are metabolites derived from ellagic acid (EA) and ellagitannins (ETs) by gut microbiota after consumption of different ETs. The health effects attributed to UROs are numerous and diverse, ranging from antimalarial properties to anticancer activities and regulation of gene expression. The aim of this work was at assessing the effect of URO-A; -B; -C; -D on the oxidative status of colon epithelium using as a model colorectal adenocarcinoma cell line (Caco-2). No significant cytotoxic effects of UROs was noted, with the applied treatments. Supplementation of cell growth medium with a mixture of UROs decreased the level of intracellular reactive oxygen species both after short- and long-term exposure. UROs also affected the activity of antioxidative enzymes within the cell, especially catalase.Conclusions: At concentrations reached in the lumen of the gut, UROs can exert beneficial effects on the cells by decreasing oxidative stress thus preventing the damage caused by reactive oxygen species.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Food Sciences and Nutrition
T1  - Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2
VL  - 68
IS  - 8
SP  - 952
EP  - 959
DO  - 10.1080/09637486.2017.1328665
ER  - 

@article{
author = "Kojadinović, Milica I. and Arsic, Aleksandra and Petovic-Oggiano, Gordana and Gavrović-Jankulović, Marija and Glibetic, Marija and Popović, Milica M.",
year = "2017",
abstract = "Urolithins (UROs) are metabolites derived from ellagic acid (EA) and ellagitannins (ETs) by gut microbiota after consumption of different ETs. The health effects attributed to UROs are numerous and diverse, ranging from antimalarial properties to anticancer activities and regulation of gene expression. The aim of this work was at assessing the effect of URO-A; -B; -C; -D on the oxidative status of colon epithelium using as a model colorectal adenocarcinoma cell line (Caco-2). No significant cytotoxic effects of UROs was noted, with the applied treatments. Supplementation of cell growth medium with a mixture of UROs decreased the level of intracellular reactive oxygen species both after short- and long-term exposure. UROs also affected the activity of antioxidative enzymes within the cell, especially catalase.Conclusions: At concentrations reached in the lumen of the gut, UROs can exert beneficial effects on the cells by decreasing oxidative stress thus preventing the damage caused by reactive oxygen species.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Food Sciences and Nutrition",
title = "Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2",
volume = "68",
number = "8",
pages = "952-959",
doi = "10.1080/09637486.2017.1328665"
}

Kojadinović, M. I., Arsic, A., Petovic-Oggiano, G., Gavrović-Jankulović, M., Glibetic, M.,& Popović, M. M.. (2017). Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2. in International Journal of Food Sciences and Nutrition
Taylor & Francis Ltd, Abingdon., 68(8), 952-959.
https://doi.org/10.1080/09637486.2017.1328665

Kojadinović MI, Arsic A, Petovic-Oggiano G, Gavrović-Jankulović M, Glibetic M, Popović MM. Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2. in International Journal of Food Sciences and Nutrition. 2017;68(8):952-959.
doi:10.1080/09637486.2017.1328665 .

Kojadinović, Milica I., Arsic, Aleksandra, Petovic-Oggiano, Gordana, Gavrović-Jankulović, Marija, Glibetic, Marija, Popović, Milica M., "Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2" in International Journal of Food Sciences and Nutrition, 68, no. 8 (2017):952-959,
https://doi.org/10.1080/09637486.2017.1328665 . .