TY  - JOUR
AU  - Mijatović, Aleksandar
AU  - Gligorijević, Nevenka
AU  - Ćoćić, Dušan
AU  - Spasić, Snežana
AU  - Lolić, Aleksandar
AU  - Aranđelović, Sandra
AU  - Nikolić, Milan
AU  - Baošić, Rada
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6248
AB  - The biological activity of six structurally similar tetradentate Schiff base copper(II) complexes, namely [Cu(ethylenediamine-bis-acetylacetonate)] (CuAA) and five derivatives where two methyl groups are replaced by phenyl, (CuPP), CF3 (CuTT) or by mixed groups CH3/CF3 (CuAT), Ph/CF3 (CuPT), and Ph/CH3 (CuAP) has been investigated. The set of antioxidant assays was performed, and the results were expressed as IC50 and EC50 values. The series of complexes showed interesting bioactivity and were investigated for the determination of antioxidant, antifungal, antimicrobial, and cytotoxic activity. A significant antioxidant behavior was exhibited by complex CuAA, greater than Trolox in the Oxygen Radical Absorbance Capacity (ORAC) assay. Antibacterial assay over Gram-positive and Gram-negative pathogenic bacterial strains and some fungal pathogens were studied. Antiproliferative activity of complexes in two human tumor cell lines, breast adenocarcinoma MCF-7, colon adenocarcinoma LS-174, and normal fibroblast cells-MRC-5, examined the effect on cell cycle progression. The significant cytotoxic potential, comparable to cisplatin cytotoxicity, was determined in human breast cancer cell line-MCF-7 with IC50 values being 17.53–31.40 μM and human colon cancer cell line-LS-174 with IC50 values being 15.22–23.92 μM. All tested compounds showed nearly twice more selectivity toward cancer cell lines than normal cells. The interactions of complexes with human serum albumin (HSA), the most prominent protein in plasma, were investigated using spectroscopic fluorescence techniques. The complexes bind to human serum albumin at multiple sites (n = 0.2–1.9), displaying a moderate binding constant Ka = 4.1–12.4 × 104 M−1. The molecular docking experiment effectively showed complex binding to HSA and DNA molecular fragments.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge
VL  - 244
SP  - 112224
DO  - 10.1016/j.jinorgbio.2023.112224
ER  - 

@article{
author = "Mijatović, Aleksandar and Gligorijević, Nevenka and Ćoćić, Dušan and Spasić, Snežana and Lolić, Aleksandar and Aranđelović, Sandra and Nikolić, Milan and Baošić, Rada",
year = "2023",
abstract = "The biological activity of six structurally similar tetradentate Schiff base copper(II) complexes, namely [Cu(ethylenediamine-bis-acetylacetonate)] (CuAA) and five derivatives where two methyl groups are replaced by phenyl, (CuPP), CF3 (CuTT) or by mixed groups CH3/CF3 (CuAT), Ph/CF3 (CuPT), and Ph/CH3 (CuAP) has been investigated. The set of antioxidant assays was performed, and the results were expressed as IC50 and EC50 values. The series of complexes showed interesting bioactivity and were investigated for the determination of antioxidant, antifungal, antimicrobial, and cytotoxic activity. A significant antioxidant behavior was exhibited by complex CuAA, greater than Trolox in the Oxygen Radical Absorbance Capacity (ORAC) assay. Antibacterial assay over Gram-positive and Gram-negative pathogenic bacterial strains and some fungal pathogens were studied. Antiproliferative activity of complexes in two human tumor cell lines, breast adenocarcinoma MCF-7, colon adenocarcinoma LS-174, and normal fibroblast cells-MRC-5, examined the effect on cell cycle progression. The significant cytotoxic potential, comparable to cisplatin cytotoxicity, was determined in human breast cancer cell line-MCF-7 with IC50 values being 17.53–31.40 μM and human colon cancer cell line-LS-174 with IC50 values being 15.22–23.92 μM. All tested compounds showed nearly twice more selectivity toward cancer cell lines than normal cells. The interactions of complexes with human serum albumin (HSA), the most prominent protein in plasma, were investigated using spectroscopic fluorescence techniques. The complexes bind to human serum albumin at multiple sites (n = 0.2–1.9), displaying a moderate binding constant Ka = 4.1–12.4 × 104 M−1. The molecular docking experiment effectively showed complex binding to HSA and DNA molecular fragments.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge",
volume = "244",
pages = "112224",
doi = "10.1016/j.jinorgbio.2023.112224"
}

Mijatović, A., Gligorijević, N., Ćoćić, D., Spasić, S., Lolić, A., Aranđelović, S., Nikolić, M.,& Baošić, R.. (2023). In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge. in Journal of Inorganic Biochemistry
Elsevier., 244, 112224.
https://doi.org/10.1016/j.jinorgbio.2023.112224

Mijatović A, Gligorijević N, Ćoćić D, Spasić S, Lolić A, Aranđelović S, Nikolić M, Baošić R. In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge. in Journal of Inorganic Biochemistry. 2023;244:112224.
doi:10.1016/j.jinorgbio.2023.112224 .

Mijatović, Aleksandar, Gligorijević, Nevenka, Ćoćić, Dušan, Spasić, Snežana, Lolić, Aleksandar, Aranđelović, Sandra, Nikolić, Milan, Baošić, Rada, "In vitro and in silico study of the biological activity of tetradentate Schiff base copper(II) complexes with ethylenediamine-bridge" in Journal of Inorganic Biochemistry, 244 (2023):112224,
https://doi.org/10.1016/j.jinorgbio.2023.112224 . .

TY  - JOUR
AU  - Mijatović, Aleksandar
AU  - Cakovic, Angelina
AU  - Lolić, Aleksandar
AU  - Sretenovic, Snezana
AU  - Zivanovic, Marko
AU  - Petrović, Biljana
AU  - Bogojeski, Jovana
AU  - Seklic, Dragana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6458
AB  - Three Schiff base Cu(II) complexes, (N,N’-bis(acetylacetone)­propyl­enediimine)copper(II) complex, [Cu(acac2pn)] (1), (N,N'-bis-(ben­zoyl­acet­one)propylenediimine)copper(II) complex, [Cu(phacac2pn)] (2) and (N,N’-bis-(trifluoroacetylacetone)propylenediimine)­copper(II) complex, [Cu(tfacac2pn)] (3), were used to investigate the interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) using the electronic absorption and spectro­scopic fluorescence methods. UV–Vis absorption studies showed that studied complexes interact with DNA molecule and exhibit moderate binding affinity. Fluorescence studies of complexes 1–3 also showed a possibility for DNA int­ercalation as well as a relatively high binding ability toward BSA. Among the tested complexes, the highest affinity for DNA and BSA molecules was shown by complex 1. Cytotoxic analyses, performed on human colorectal carcinoma HCT-116 and healthy lung fibroblast MRC-5 cell lines, showed that complex 2 exhibited activity on both cell lines, while complexes 1 and 3 did not show any activity.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - DNA/BSA interactions and cytotoxic studies of tetradentate N,N,O,O-Schiff base copper(II) complexes: Scientific paper
VL  - 88
IS  - 12
SP  - 1307
EP  - 1317
DO  - 10.2298/JSC230614063M
ER  - 

@article{
author = "Mijatović, Aleksandar and Cakovic, Angelina and Lolić, Aleksandar and Sretenovic, Snezana and Zivanovic, Marko and Petrović, Biljana and Bogojeski, Jovana and Seklic, Dragana",
year = "2023",
abstract = "Three Schiff base Cu(II) complexes, (N,N’-bis(acetylacetone)­propyl­enediimine)copper(II) complex, [Cu(acac2pn)] (1), (N,N'-bis-(ben­zoyl­acet­one)propylenediimine)copper(II) complex, [Cu(phacac2pn)] (2) and (N,N’-bis-(trifluoroacetylacetone)propylenediimine)­copper(II) complex, [Cu(tfacac2pn)] (3), were used to investigate the interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) using the electronic absorption and spectro­scopic fluorescence methods. UV–Vis absorption studies showed that studied complexes interact with DNA molecule and exhibit moderate binding affinity. Fluorescence studies of complexes 1–3 also showed a possibility for DNA int­ercalation as well as a relatively high binding ability toward BSA. Among the tested complexes, the highest affinity for DNA and BSA molecules was shown by complex 1. Cytotoxic analyses, performed on human colorectal carcinoma HCT-116 and healthy lung fibroblast MRC-5 cell lines, showed that complex 2 exhibited activity on both cell lines, while complexes 1 and 3 did not show any activity.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "DNA/BSA interactions and cytotoxic studies of tetradentate N,N,O,O-Schiff base copper(II) complexes: Scientific paper",
volume = "88",
number = "12",
pages = "1307-1317",
doi = "10.2298/JSC230614063M"
}

Mijatović, A., Cakovic, A., Lolić, A., Sretenovic, S., Zivanovic, M., Petrović, B., Bogojeski, J.,& Seklic, D.. (2023). DNA/BSA interactions and cytotoxic studies of tetradentate N,N,O,O-Schiff base copper(II) complexes: Scientific paper. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(12), 1307-1317.
https://doi.org/10.2298/JSC230614063M

Mijatović A, Cakovic A, Lolić A, Sretenovic S, Zivanovic M, Petrović B, Bogojeski J, Seklic D. DNA/BSA interactions and cytotoxic studies of tetradentate N,N,O,O-Schiff base copper(II) complexes: Scientific paper. in Journal of the Serbian Chemical Society. 2023;88(12):1307-1317.
doi:10.2298/JSC230614063M .

Mijatović, Aleksandar, Cakovic, Angelina, Lolić, Aleksandar, Sretenovic, Snezana, Zivanovic, Marko, Petrović, Biljana, Bogojeski, Jovana, Seklic, Dragana, "DNA/BSA interactions and cytotoxic studies of tetradentate N,N,O,O-Schiff base copper(II) complexes: Scientific paper" in Journal of the Serbian Chemical Society, 88, no. 12 (2023):1307-1317,
https://doi.org/10.2298/JSC230614063M . .

TY  - JOUR
AU  - Kulizić, Mihajlo N.
AU  - Stanković, Milan
AU  - Rašić, Marija
AU  - Mijatović, Aleksandar
AU  - Baošić, Rada
AU  - Lolić, Aleksandar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6431
AB  - In this paper, a simple, sensitive, and precise flow injection system was optimized and applied to ascorbic acid determination with a carbon paste electrode modified with the Schiff base copper complex. The modified sensor showed increased sensitivity toward ascorbic acid oxidation with a decrease of oxidation potential by 200 mV. Under optimized flow parameters, working potential, gasket thickness, sample and reaction coil volumes, and flow rate, the developed flow system gave a linear response from 5 to 75 μmol/L of ascorbic acid. The calculated limit of detection was 4.5 μmol/L, calculated as 3σ/s which corresponds to the absolute value of 40 ng for the 50 μL sample coil. The precision for six consecutive injections of 5 and 75 μmol/L ascorbic acid solutions was determined as a relative standard deviation of 5.8 % and 1.5 %, respectively. The flow injection system enabled up to 120 analyses per hour. An optimized flow system was applied to the determination of ascorbic acid in dietary supplements, after simple preparation.
PB  - Wiley
T1  - A Simple Flow Injection System for Amperometric Detection of Ascorbic Acid using Carbon Paste/Copper Schiff Base Composite Electrode
VL  - 8
IS  - 48
SP  - e202304631
DO  - 10.1002/slct.202304631
ER  - 

@article{
author = "Kulizić, Mihajlo N. and Stanković, Milan and Rašić, Marija and Mijatović, Aleksandar and Baošić, Rada and Lolić, Aleksandar",
year = "2023",
abstract = "In this paper, a simple, sensitive, and precise flow injection system was optimized and applied to ascorbic acid determination with a carbon paste electrode modified with the Schiff base copper complex. The modified sensor showed increased sensitivity toward ascorbic acid oxidation with a decrease of oxidation potential by 200 mV. Under optimized flow parameters, working potential, gasket thickness, sample and reaction coil volumes, and flow rate, the developed flow system gave a linear response from 5 to 75 μmol/L of ascorbic acid. The calculated limit of detection was 4.5 μmol/L, calculated as 3σ/s which corresponds to the absolute value of 40 ng for the 50 μL sample coil. The precision for six consecutive injections of 5 and 75 μmol/L ascorbic acid solutions was determined as a relative standard deviation of 5.8 % and 1.5 %, respectively. The flow injection system enabled up to 120 analyses per hour. An optimized flow system was applied to the determination of ascorbic acid in dietary supplements, after simple preparation.",
publisher = "Wiley",
title = "A Simple Flow Injection System for Amperometric Detection of Ascorbic Acid using Carbon Paste/Copper Schiff Base Composite Electrode",
volume = "8",
number = "48",
pages = "e202304631",
doi = "10.1002/slct.202304631"
}

Kulizić, M. N., Stanković, M., Rašić, M., Mijatović, A., Baošić, R.,& Lolić, A.. (2023). A Simple Flow Injection System for Amperometric Detection of Ascorbic Acid using Carbon Paste/Copper Schiff Base Composite Electrode. 
Wiley., 8(48), e202304631.
https://doi.org/10.1002/slct.202304631

Kulizić MN, Stanković M, Rašić M, Mijatović A, Baošić R, Lolić A. A Simple Flow Injection System for Amperometric Detection of Ascorbic Acid using Carbon Paste/Copper Schiff Base Composite Electrode. 2023;8(48):e202304631.
doi:10.1002/slct.202304631 .

Kulizić, Mihajlo N., Stanković, Milan, Rašić, Marija, Mijatović, Aleksandar, Baošić, Rada, Lolić, Aleksandar, "A Simple Flow Injection System for Amperometric Detection of Ascorbic Acid using Carbon Paste/Copper Schiff Base Composite Electrode", 8, no. 48 (2023):e202304631,
https://doi.org/10.1002/slct.202304631 . .

TY  - JOUR
AU  - Mijin, Nemanja
AU  - Milošević, Jelica
AU  - Stevanović, Sanja
AU  - Petrović, Predrag
AU  - Lolić, Aleksandar
AU  - Urbic, Tomaz
AU  - Polović, Natalija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5987
AB  - The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.
PB  - Elsevier B.V.
T2  - Food Hydrocolloids
T1  - Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin
VL  - 136
SP  - 108292
DO  - 10.1016/j.foodhyd.2022.108292
ER  - 

@article{
author = "Mijin, Nemanja and Milošević, Jelica and Stevanović, Sanja and Petrović, Predrag and Lolić, Aleksandar and Urbic, Tomaz and Polović, Natalija",
year = "2023",
abstract = "The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.",
publisher = "Elsevier B.V.",
journal = "Food Hydrocolloids",
title = "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin",
volume = "136",
pages = "108292",
doi = "10.1016/j.foodhyd.2022.108292"
}

Mijin, N., Milošević, J., Stevanović, S., Petrović, P., Lolić, A., Urbic, T.,& Polović, N.. (2023). Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids
Elsevier B.V.., 136, 108292.
https://doi.org/10.1016/j.foodhyd.2022.108292

Mijin N, Milošević J, Stevanović S, Petrović P, Lolić A, Urbic T, Polović N. Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids. 2023;136:108292.
doi:10.1016/j.foodhyd.2022.108292 .

Mijin, Nemanja, Milošević, Jelica, Stevanović, Sanja, Petrović, Predrag, Lolić, Aleksandar, Urbic, Tomaz, Polović, Natalija, "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin" in Food Hydrocolloids, 136 (2023):108292,
https://doi.org/10.1016/j.foodhyd.2022.108292 . .

TY  - JOUR
AU  - Antunović, Vesna
AU  - Blagojević, Dragana
AU  - Baošić, Rada
AU  - Relić, Dubravka
AU  - Lolić, Aleksandar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6252
AB  - This study assesses heavy metal content in soil, water, and plant material from sites located around the lignite mine and the power plant “Gacko”, Bosnia and Herzegovina. The samples were collected, prepared, and analyzed for heavy metals content using the flame atomic absorption spectrophotometer. Samples were analyzed for cadmium, lead, copper, zinc, manganese, and iron. To identify the relationship among the metals in samples and their possible sources, Pearson’s correlation and principal component analysis were performed. Health risk assessment was applied to establish potential health risks posed to humans caused by contaminants in different environmental compartments. The results of our analyses show that most soil samples contain copper, and one of those samples had a copper concentration of more than 70 µg/g, which is a critical upper value for agricultural use. In the soil samples that were analyzed, cadmium was also detected, and its concentration was greater than 2 µg/g. Lead, on the other hand, had a concentration that was higher than the maximum permissible for unpolluted soils in 40% of the soil samples that were analyzed. Lead and cadmium concentrations in surface waters mostly contribute to a non-carcinogenic risk in the scenario of recreational swimming exposure. The presence of Cd, a highly toxic element in water, may be explained by the leaching of artificial fertilizers used in the study area, whereas Pb’s origin may be geological. The results of this study recommend routine heavy metal monitoring in samples of soil, water, and plants from the examined area so that, if metal concentrations continue increasing, remedial action should be advised to prevent accumulation in the food chain.
PB  - Springer
T2  - Environmental Monitoring and Assessment
T1  - Health risk assessment of heavy metals in soil, plant, and water samples near “Gacko” power plant, in Bosnia and Herzegovina
VL  - 195
IS  - 5
SP  - 596
DO  - 10.1007/s10661-023-11232-7
ER  - 

@article{
author = "Antunović, Vesna and Blagojević, Dragana and Baošić, Rada and Relić, Dubravka and Lolić, Aleksandar",
year = "2023",
abstract = "This study assesses heavy metal content in soil, water, and plant material from sites located around the lignite mine and the power plant “Gacko”, Bosnia and Herzegovina. The samples were collected, prepared, and analyzed for heavy metals content using the flame atomic absorption spectrophotometer. Samples were analyzed for cadmium, lead, copper, zinc, manganese, and iron. To identify the relationship among the metals in samples and their possible sources, Pearson’s correlation and principal component analysis were performed. Health risk assessment was applied to establish potential health risks posed to humans caused by contaminants in different environmental compartments. The results of our analyses show that most soil samples contain copper, and one of those samples had a copper concentration of more than 70 µg/g, which is a critical upper value for agricultural use. In the soil samples that were analyzed, cadmium was also detected, and its concentration was greater than 2 µg/g. Lead, on the other hand, had a concentration that was higher than the maximum permissible for unpolluted soils in 40% of the soil samples that were analyzed. Lead and cadmium concentrations in surface waters mostly contribute to a non-carcinogenic risk in the scenario of recreational swimming exposure. The presence of Cd, a highly toxic element in water, may be explained by the leaching of artificial fertilizers used in the study area, whereas Pb’s origin may be geological. The results of this study recommend routine heavy metal monitoring in samples of soil, water, and plants from the examined area so that, if metal concentrations continue increasing, remedial action should be advised to prevent accumulation in the food chain.",
publisher = "Springer",
journal = "Environmental Monitoring and Assessment",
title = "Health risk assessment of heavy metals in soil, plant, and water samples near “Gacko” power plant, in Bosnia and Herzegovina",
volume = "195",
number = "5",
pages = "596",
doi = "10.1007/s10661-023-11232-7"
}

Antunović, V., Blagojević, D., Baošić, R., Relić, D.,& Lolić, A.. (2023). Health risk assessment of heavy metals in soil, plant, and water samples near “Gacko” power plant, in Bosnia and Herzegovina. in Environmental Monitoring and Assessment
Springer., 195(5), 596.
https://doi.org/10.1007/s10661-023-11232-7

Antunović V, Blagojević D, Baošić R, Relić D, Lolić A. Health risk assessment of heavy metals in soil, plant, and water samples near “Gacko” power plant, in Bosnia and Herzegovina. in Environmental Monitoring and Assessment. 2023;195(5):596.
doi:10.1007/s10661-023-11232-7 .

Antunović, Vesna, Blagojević, Dragana, Baošić, Rada, Relić, Dubravka, Lolić, Aleksandar, "Health risk assessment of heavy metals in soil, plant, and water samples near “Gacko” power plant, in Bosnia and Herzegovina" in Environmental Monitoring and Assessment, 195, no. 5 (2023):596,
https://doi.org/10.1007/s10661-023-11232-7 . .

TY  - JOUR
AU  - Matijašević, Igor
AU  - Kulizić, Mihajlo N.
AU  - Bacetić, Ljubica
AU  - Gavrilović, Damjan
AU  - Baošić, Rada
AU  - Lolić, Aleksandar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6268
AB  - A simple modification of bare glassy carbon electrode by electropolymerization of glycine by cyclic voltammetry was applied to the detection of ibuprofen. The polyglycine modified glassy carbon electrode was characterized by cyclic voltammetry and the electroactive surface area was determined. The study of ibuprofen in acetic acid buffer (0.25 M, pH 4.0) presented an electrocatalytic effect toward ibuprofen oxidation, increasing the current response and decrease of oxidation potential, at the modified electrode compared to the bare. Under the optimal conditions, the modified electrode gave a linear response in the ibuprofen concentration range of 10 to 200 μM, obtained correlation coefficient was 0.9993. the calculated limit of detection (3Sb/m) was 1.0 μM and the limit of quantification (10Sb/m) was 3.5 μM. the sensor was successfully used for the determination of ibuprofen in pharmaceutical preparations with satisfactory recoveries, 93–107 %. The new sensor showed good selectivity, repeatability, and reproducibility with satisfactory stability.
PB  - John Wiley and Sons Inc
T2  - ChemistrySelect
T1  - Polyglycine Modified Glassy Carbon Electrode for Ibuprofen Determination
VL  - 8
IS  - 24
SP  - e202300827
DO  - 10.1002/slct.202300827
ER  - 

@article{
author = "Matijašević, Igor and Kulizić, Mihajlo N. and Bacetić, Ljubica and Gavrilović, Damjan and Baošić, Rada and Lolić, Aleksandar",
year = "2023",
abstract = "A simple modification of bare glassy carbon electrode by electropolymerization of glycine by cyclic voltammetry was applied to the detection of ibuprofen. The polyglycine modified glassy carbon electrode was characterized by cyclic voltammetry and the electroactive surface area was determined. The study of ibuprofen in acetic acid buffer (0.25 M, pH 4.0) presented an electrocatalytic effect toward ibuprofen oxidation, increasing the current response and decrease of oxidation potential, at the modified electrode compared to the bare. Under the optimal conditions, the modified electrode gave a linear response in the ibuprofen concentration range of 10 to 200 μM, obtained correlation coefficient was 0.9993. the calculated limit of detection (3Sb/m) was 1.0 μM and the limit of quantification (10Sb/m) was 3.5 μM. the sensor was successfully used for the determination of ibuprofen in pharmaceutical preparations with satisfactory recoveries, 93–107 %. The new sensor showed good selectivity, repeatability, and reproducibility with satisfactory stability.",
publisher = "John Wiley and Sons Inc",
journal = "ChemistrySelect",
title = "Polyglycine Modified Glassy Carbon Electrode for Ibuprofen Determination",
volume = "8",
number = "24",
pages = "e202300827",
doi = "10.1002/slct.202300827"
}

Matijašević, I., Kulizić, M. N., Bacetić, L., Gavrilović, D., Baošić, R.,& Lolić, A.. (2023). Polyglycine Modified Glassy Carbon Electrode for Ibuprofen Determination. in ChemistrySelect
John Wiley and Sons Inc., 8(24), e202300827.
https://doi.org/10.1002/slct.202300827

Matijašević I, Kulizić MN, Bacetić L, Gavrilović D, Baošić R, Lolić A. Polyglycine Modified Glassy Carbon Electrode for Ibuprofen Determination. in ChemistrySelect. 2023;8(24):e202300827.
doi:10.1002/slct.202300827 .

Matijašević, Igor, Kulizić, Mihajlo N., Bacetić, Ljubica, Gavrilović, Damjan, Baošić, Rada, Lolić, Aleksandar, "Polyglycine Modified Glassy Carbon Electrode for Ibuprofen Determination" in ChemistrySelect, 8, no. 24 (2023):e202300827,
https://doi.org/10.1002/slct.202300827 . .

TY  - JOUR
AU  - Đogo-Mračević, Svetlana
AU  - Laketić, Tatjana
AU  - Stanković, Milan
AU  - Lolić, Aleksandar
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6322
AB  - The aim of this research was to determine the content of arsenic (As), cadmium (Cd), lead (Pb), and nickel (Ni) in selected lipsticks and face foundations of different price categories and the health risk assessment associated with these toxic elements via dermal and oral exposure. The samples (10 lipstick and 10 face foundations) were purchased from local markets in Belgrade, Serbia. The samples were prepared by acid digestion and analyzed by atomic absorption spectrometry (flame atomization for Cd, Pb, and Ni and electrothermal atomization for As). Arsenic was found in 50% of lipstick samples and in only two face foundation samples with the highest concentration of 0.28 mg/kg in the lipstick sample. Lead concentration was in the range of 1.15 to 5.12 mg/kg in ten samples. Nickel was found in five samples out of 20, with the highest concentration of 4.20 mg/kg. Cadmium was found in only one face foundation sample in the cheaper price range (1.11 mg/kg). The obtained results indicate that concentrations of As, Pb, Cd, and Ni were within the permissible limits according to Serbian legislation. Health risk associated with these elements was assessed as non-carcinogenic and carcinogenic risk. Obtained hazard quotients (HQs) and hazard indexes (HIs) were below 1, indicating the absence of significant non-carcinogenic health risk due to exposure to these toxic elements (TEs). The carcinogenic risk results show that investigated cosmetic products can be considered low-risky due to the possibility of causing malignant disease.
PB  - Springer Nature
T2  - Environmental Monitoring and Assessment
T1  - Toxic element determination in selected cosmetic products: health risk assessment
VL  - 195
IS  - 9
DO  - 10.1007/s10661-023-11664-1
ER  - 

@article{
author = "Đogo-Mračević, Svetlana and Laketić, Tatjana and Stanković, Milan and Lolić, Aleksandar",
year = "2023",
abstract = "The aim of this research was to determine the content of arsenic (As), cadmium (Cd), lead (Pb), and nickel (Ni) in selected lipsticks and face foundations of different price categories and the health risk assessment associated with these toxic elements via dermal and oral exposure. The samples (10 lipstick and 10 face foundations) were purchased from local markets in Belgrade, Serbia. The samples were prepared by acid digestion and analyzed by atomic absorption spectrometry (flame atomization for Cd, Pb, and Ni and electrothermal atomization for As). Arsenic was found in 50% of lipstick samples and in only two face foundation samples with the highest concentration of 0.28 mg/kg in the lipstick sample. Lead concentration was in the range of 1.15 to 5.12 mg/kg in ten samples. Nickel was found in five samples out of 20, with the highest concentration of 4.20 mg/kg. Cadmium was found in only one face foundation sample in the cheaper price range (1.11 mg/kg). The obtained results indicate that concentrations of As, Pb, Cd, and Ni were within the permissible limits according to Serbian legislation. Health risk associated with these elements was assessed as non-carcinogenic and carcinogenic risk. Obtained hazard quotients (HQs) and hazard indexes (HIs) were below 1, indicating the absence of significant non-carcinogenic health risk due to exposure to these toxic elements (TEs). The carcinogenic risk results show that investigated cosmetic products can be considered low-risky due to the possibility of causing malignant disease.",
publisher = "Springer Nature",
journal = "Environmental Monitoring and Assessment",
title = "Toxic element determination in selected cosmetic products: health risk assessment",
volume = "195",
number = "9",
doi = "10.1007/s10661-023-11664-1"
}

Đogo-Mračević, S., Laketić, T., Stanković, M.,& Lolić, A.. (2023). Toxic element determination in selected cosmetic products: health risk assessment. in Environmental Monitoring and Assessment
Springer Nature., 195(9).
https://doi.org/10.1007/s10661-023-11664-1

Đogo-Mračević S, Laketić T, Stanković M, Lolić A. Toxic element determination in selected cosmetic products: health risk assessment. in Environmental Monitoring and Assessment. 2023;195(9).
doi:10.1007/s10661-023-11664-1 .

Đogo-Mračević, Svetlana, Laketić, Tatjana, Stanković, Milan, Lolić, Aleksandar, "Toxic element determination in selected cosmetic products: health risk assessment" in Environmental Monitoring and Assessment, 195, no. 9 (2023),
https://doi.org/10.1007/s10661-023-11664-1 . .

TY  - JOUR
AU  - Stevanović, Nikola R.
AU  - Mijatović, Aleksandar
AU  - Lolić, Aleksandar
AU  - Zlatović, Mario
AU  - Baošić, Rada
PY  - 2022
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4707
AB  - The influences of the application of mono- and two-component organic modifiers on lipophilicity determination of 12 tetradentate Schiff bases by reversed-phase thin layer chromatography were investigated. The main goal is to estimate types of interaction between observed compounds and components of the applied chromatographic systems and establish some behaviour pattern in order to easier choose a combination of organic modifiers which will simulate interaction in biological systems based on the facts that the same basic intermolecular interactions are responsible for the behaviour of substances in both the biological and chromatographic system. The applied organic modifier shows the ability to modify the surface of the applied sorbent, which affects the manifestation of lipophilicity of the observed compounds. Mono-component organic modifiers from different groups of the Snyder triangle were used, as well as their two-component mixtures. In addition, we compared experimentally determined calculated parameters of lipophilicity.
PB  - Springer
T2  - Chemical Papers
T1  - Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases
VL  - 76
SP  - 585
EP  - 593
DO  - 10.1007/s11696-021-01884-5
ER  - 

@article{
author = "Stevanović, Nikola R. and Mijatović, Aleksandar and Lolić, Aleksandar and Zlatović, Mario and Baošić, Rada",
year = "2022",
abstract = "The influences of the application of mono- and two-component organic modifiers on lipophilicity determination of 12 tetradentate Schiff bases by reversed-phase thin layer chromatography were investigated. The main goal is to estimate types of interaction between observed compounds and components of the applied chromatographic systems and establish some behaviour pattern in order to easier choose a combination of organic modifiers which will simulate interaction in biological systems based on the facts that the same basic intermolecular interactions are responsible for the behaviour of substances in both the biological and chromatographic system. The applied organic modifier shows the ability to modify the surface of the applied sorbent, which affects the manifestation of lipophilicity of the observed compounds. Mono-component organic modifiers from different groups of the Snyder triangle were used, as well as their two-component mixtures. In addition, we compared experimentally determined calculated parameters of lipophilicity.",
publisher = "Springer",
journal = "Chemical Papers",
title = "Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases",
volume = "76",
pages = "585-593",
doi = "10.1007/s11696-021-01884-5"
}

Stevanović, N. R., Mijatović, A., Lolić, A., Zlatović, M.,& Baošić, R.. (2022). Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases. in Chemical Papers
Springer., 76, 585-593.
https://doi.org/10.1007/s11696-021-01884-5

Stevanović NR, Mijatović A, Lolić A, Zlatović M, Baošić R. Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases. in Chemical Papers. 2022;76:585-593.
doi:10.1007/s11696-021-01884-5 .

Stevanović, Nikola R., Mijatović, Aleksandar, Lolić, Aleksandar, Zlatović, Mario, Baošić, Rada, "Influence of mono- and two-component organic modifiers on determination of lipophilicity of tetradentate Schiff bases" in Chemical Papers, 76 (2022):585-593,
https://doi.org/10.1007/s11696-021-01884-5 . .

TY  - JOUR
AU  - Tripković, Tatjana
AU  - Vasić, Ružica
AU  - Lolić, Aleksandar
AU  - Baošić, Rada
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4948
AB  - Graphite furnace atomic absorption spectroscopy (GFAAS) was applied to identification of historical pigments. The method is adapted to determine different metals, usually found in pigments. Operating conditions were optimized, such as sample drying step, pyrolysis and atomization temperatures, modifiers, and volume of sample introduced into the atomizer, to reduce interferences due to a complex sample matrix. Using the optimized method pigments in samples from the painting Coronation of the Virgin from the Church of the Shroud of the Holy Virgin in Barič were identified. The samples were analyzed also by Raman microscopy to verify the identification achieved by GFAAS. Raman microscopy confirmed the assumptionsmade based on GFAAS results. Using the two techniques a variety of pigments have been identified, such as calcite, leadwhite, goethite, hematite, ultramarine, vermilion, minium, carbon black. Based on GFAAS results the presence of azuritewas observed in the blue paint sample, although this finding was not confirmed by Raman microscopy.
PB  - Springer
T2  - Chemical Papers
T1  - Determination of metals in artistic pigments using the optimized GFAAS method and Raman spectroscopy
VL  - 76
SP  - 3607
EP  - 3618
DO  - 10.1007/s11696-022-02110-6
ER  - 

@article{
author = "Tripković, Tatjana and Vasić, Ružica and Lolić, Aleksandar and Baošić, Rada",
year = "2022",
abstract = "Graphite furnace atomic absorption spectroscopy (GFAAS) was applied to identification of historical pigments. The method is adapted to determine different metals, usually found in pigments. Operating conditions were optimized, such as sample drying step, pyrolysis and atomization temperatures, modifiers, and volume of sample introduced into the atomizer, to reduce interferences due to a complex sample matrix. Using the optimized method pigments in samples from the painting Coronation of the Virgin from the Church of the Shroud of the Holy Virgin in Barič were identified. The samples were analyzed also by Raman microscopy to verify the identification achieved by GFAAS. Raman microscopy confirmed the assumptionsmade based on GFAAS results. Using the two techniques a variety of pigments have been identified, such as calcite, leadwhite, goethite, hematite, ultramarine, vermilion, minium, carbon black. Based on GFAAS results the presence of azuritewas observed in the blue paint sample, although this finding was not confirmed by Raman microscopy.",
publisher = "Springer",
journal = "Chemical Papers",
title = "Determination of metals in artistic pigments using the optimized GFAAS method and Raman spectroscopy",
volume = "76",
pages = "3607-3618",
doi = "10.1007/s11696-022-02110-6"
}

Tripković, T., Vasić, R., Lolić, A.,& Baošić, R.. (2022). Determination of metals in artistic pigments using the optimized GFAAS method and Raman spectroscopy. in Chemical Papers
Springer., 76, 3607-3618.
https://doi.org/10.1007/s11696-022-02110-6

Tripković T, Vasić R, Lolić A, Baošić R. Determination of metals in artistic pigments using the optimized GFAAS method and Raman spectroscopy. in Chemical Papers. 2022;76:3607-3618.
doi:10.1007/s11696-022-02110-6 .

Tripković, Tatjana, Vasić, Ružica, Lolić, Aleksandar, Baošić, Rada, "Determination of metals in artistic pigments using the optimized GFAAS method and Raman spectroscopy" in Chemical Papers, 76 (2022):3607-3618,
https://doi.org/10.1007/s11696-022-02110-6 . .

TY  - DATA
AU  - Tripković, Tatjana
AU  - Vasić, Ružica
AU  - Lolić, Aleksandar
AU  - Baošić, Rada
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4949
PB  - Springer
T2  - Chemical Papers
T1  - Supplementary data for the article: Tripković, T.; Vasić, R.; Lolić, A.; Baošić, R. Determination of Metals in Artistic Pigments Using the Optimized GFAAS Method and Raman Spectroscopy. Chemical Papers 2022, n/a. https://doi.org/10.1007/s11696-022-02110-6.
VL  - 76
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4949
ER  - 

@misc{
author = "Tripković, Tatjana and Vasić, Ružica and Lolić, Aleksandar and Baošić, Rada",
year = "2022",
publisher = "Springer",
journal = "Chemical Papers",
title = "Supplementary data for the article: Tripković, T.; Vasić, R.; Lolić, A.; Baošić, R. Determination of Metals in Artistic Pigments Using the Optimized GFAAS Method and Raman Spectroscopy. Chemical Papers 2022, n/a. https://doi.org/10.1007/s11696-022-02110-6.",
volume = "76",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4949"
}

Tripković, T., Vasić, R., Lolić, A.,& Baošić, R.. (2022). Supplementary data for the article: Tripković, T.; Vasić, R.; Lolić, A.; Baošić, R. Determination of Metals in Artistic Pigments Using the Optimized GFAAS Method and Raman Spectroscopy. Chemical Papers 2022, n/a. https://doi.org/10.1007/s11696-022-02110-6.. in Chemical Papers
Springer., 76.
https://hdl.handle.net/21.15107/rcub_cherry_4949

Tripković T, Vasić R, Lolić A, Baošić R. Supplementary data for the article: Tripković, T.; Vasić, R.; Lolić, A.; Baošić, R. Determination of Metals in Artistic Pigments Using the Optimized GFAAS Method and Raman Spectroscopy. Chemical Papers 2022, n/a. https://doi.org/10.1007/s11696-022-02110-6.. in Chemical Papers. 2022;76.
https://hdl.handle.net/21.15107/rcub_cherry_4949 .

Tripković, Tatjana, Vasić, Ružica, Lolić, Aleksandar, Baošić, Rada, "Supplementary data for the article: Tripković, T.; Vasić, R.; Lolić, A.; Baošić, R. Determination of Metals in Artistic Pigments Using the Optimized GFAAS Method and Raman Spectroscopy. Chemical Papers 2022, n/a. https://doi.org/10.1007/s11696-022-02110-6." in Chemical Papers, 76 (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4949 .

TY  - JOUR
AU  - Ognjanović, Miloš
AU  - Nikolić, Katarina
AU  - Radenković, Marina
AU  - Lolić, Aleksandar
AU  - Stanković, Dalibor
AU  - Živković, Sanja
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5675
AB  - Gallic acid (GA) is one of the most important and present natural phenolic compounds due to its well-known biological properties, and its detection and monitoring is of great importance. Silver nanoparticles (AgNPs) are one of the most studied metallic nanomaterials used in various fields, from biomedical applications to electrochemical sensing devices. In this work, we used the pulsed laser ablation technique in liquid for the one-step preparation of nanoparticles of silver from a pure silver plate base in N,N-dimethylformamide. Obtained nanomaterial was characterized using morphological and electrochemical methods and used for modification of screen-printed carbon electrodes (SPCE). Successful immobilization at the surface is confirmed using the surface profiling method. A newly obtained sensor was used for the detection of GA. After parameters optimization, a differential pulse voltammetric protocol was developed, using two approaches - concentration vs. current (GA determination) and peak area vs. current (estimation of the antioxidant capacity). For the first approach sensor linearity was found to be in the range from 0.50 µM to 10 µM, with the limit of detection (LOD) of 0.16 µM and limit of quantification (LOQ) of 0.50 µM. In the second system operating linear range was the same, while LOD and LOQ were 0.11 µM and 0.34 µM, respectively. Practical application of the method was tested using two approaches: direct measurement of gallic acid in biological fluids and estimation of the antioxidant capacity and food quality purpose.
PB  - Elsevier
T2  - Surfaces and Interfaces
T1  - Picosecond laser-assisted synthesis of silver nanoparticles with high practical application as electroanalytical sensor
VL  - 35
SP  - 102464
DO  - 10.1016/j.surfin.2022.102464
ER  - 

@article{
author = "Ognjanović, Miloš and Nikolić, Katarina and Radenković, Marina and Lolić, Aleksandar and Stanković, Dalibor and Živković, Sanja",
year = "2022",
abstract = "Gallic acid (GA) is one of the most important and present natural phenolic compounds due to its well-known biological properties, and its detection and monitoring is of great importance. Silver nanoparticles (AgNPs) are one of the most studied metallic nanomaterials used in various fields, from biomedical applications to electrochemical sensing devices. In this work, we used the pulsed laser ablation technique in liquid for the one-step preparation of nanoparticles of silver from a pure silver plate base in N,N-dimethylformamide. Obtained nanomaterial was characterized using morphological and electrochemical methods and used for modification of screen-printed carbon electrodes (SPCE). Successful immobilization at the surface is confirmed using the surface profiling method. A newly obtained sensor was used for the detection of GA. After parameters optimization, a differential pulse voltammetric protocol was developed, using two approaches - concentration vs. current (GA determination) and peak area vs. current (estimation of the antioxidant capacity). For the first approach sensor linearity was found to be in the range from 0.50 µM to 10 µM, with the limit of detection (LOD) of 0.16 µM and limit of quantification (LOQ) of 0.50 µM. In the second system operating linear range was the same, while LOD and LOQ were 0.11 µM and 0.34 µM, respectively. Practical application of the method was tested using two approaches: direct measurement of gallic acid in biological fluids and estimation of the antioxidant capacity and food quality purpose.",
publisher = "Elsevier",
journal = "Surfaces and Interfaces",
title = "Picosecond laser-assisted synthesis of silver nanoparticles with high practical application as electroanalytical sensor",
volume = "35",
pages = "102464",
doi = "10.1016/j.surfin.2022.102464"
}

Ognjanović, M., Nikolić, K., Radenković, M., Lolić, A., Stanković, D.,& Živković, S.. (2022). Picosecond laser-assisted synthesis of silver nanoparticles with high practical application as electroanalytical sensor. in Surfaces and Interfaces
Elsevier., 35, 102464.
https://doi.org/10.1016/j.surfin.2022.102464

Ognjanović M, Nikolić K, Radenković M, Lolić A, Stanković D, Živković S. Picosecond laser-assisted synthesis of silver nanoparticles with high practical application as electroanalytical sensor. in Surfaces and Interfaces. 2022;35:102464.
doi:10.1016/j.surfin.2022.102464 .

Ognjanović, Miloš, Nikolić, Katarina, Radenković, Marina, Lolić, Aleksandar, Stanković, Dalibor, Živković, Sanja, "Picosecond laser-assisted synthesis of silver nanoparticles with high practical application as electroanalytical sensor" in Surfaces and Interfaces, 35 (2022):102464,
https://doi.org/10.1016/j.surfin.2022.102464 . .

TY  - JOUR
AU  - Ilić, Marija
AU  - Haegel, Franz-Hubert
AU  - Lolić, Aleksandar
AU  - Nedić, Zoran
AU  - Tosti, Tomislav
AU  - Ignjatović, Ivana Sredović
AU  - Sredović Ignjatović, Ivana
AU  - Linden, Andreas
AU  - Jablonowski, Nicolai D.
AU  - Hartmann, Heinrich
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5679
AB  - The knowledge of the structural and chemical properties of biochars is decisive for their application as technical products. For this reason, methods for the characterization of biochars that are generally applicable and allow quality control are highly desired. Several methods that have shown potential in other studies were used to investigate two activated carbons and seven biochars from different processes and feedstock. The chars were chosen to cover a wide range of chemical composition and structural properties as a hardness test for the analytical methods used in this study. Specific problems connected with the pretreatment of samples and drawbacks of some methods for some types of chars could be identified in an integrated consideration of the results from different methods. None of the spectroscopic methods was found to be suitable for the quality control of all types of chars. The most valuable results were obtained by chemical analysis that, however, required the complete determination of the main elements, including that of oxygen, and of inorganic components for adequate results. The combination of X-ray photoelectron spectroscopy (XPS) and FT-IR spectroscopy allows a rough characterization of surface functional groups, but cannot discriminate aliphatic and aromatic OH groups. FT-IR might be a suitable method for the quality control of biochars made at lower temperature. The results of Raman spectroscopy did not well correlate with the amount of sp2 hybridized carbon determined by XPS. A better correlation of XPS data was found with the electrical polarization determined by the method of spectral induced polarization that was used for the first time in conjunction with extensive analytical characterization.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - Surface functional groups and degree of carbonization of selected chars from different processes and feedstock
VL  - 17
IS  - 11
SP  - e0277365
DO  - 10.1371/journal.pone.0277365
ER  - 

@article{
author = "Ilić, Marija and Haegel, Franz-Hubert and Lolić, Aleksandar and Nedić, Zoran and Tosti, Tomislav and Ignjatović, Ivana Sredović and Sredović Ignjatović, Ivana and Linden, Andreas and Jablonowski, Nicolai D. and Hartmann, Heinrich",
year = "2022",
abstract = "The knowledge of the structural and chemical properties of biochars is decisive for their application as technical products. For this reason, methods for the characterization of biochars that are generally applicable and allow quality control are highly desired. Several methods that have shown potential in other studies were used to investigate two activated carbons and seven biochars from different processes and feedstock. The chars were chosen to cover a wide range of chemical composition and structural properties as a hardness test for the analytical methods used in this study. Specific problems connected with the pretreatment of samples and drawbacks of some methods for some types of chars could be identified in an integrated consideration of the results from different methods. None of the spectroscopic methods was found to be suitable for the quality control of all types of chars. The most valuable results were obtained by chemical analysis that, however, required the complete determination of the main elements, including that of oxygen, and of inorganic components for adequate results. The combination of X-ray photoelectron spectroscopy (XPS) and FT-IR spectroscopy allows a rough characterization of surface functional groups, but cannot discriminate aliphatic and aromatic OH groups. FT-IR might be a suitable method for the quality control of biochars made at lower temperature. The results of Raman spectroscopy did not well correlate with the amount of sp2 hybridized carbon determined by XPS. A better correlation of XPS data was found with the electrical polarization determined by the method of spectral induced polarization that was used for the first time in conjunction with extensive analytical characterization.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "Surface functional groups and degree of carbonization of selected chars from different processes and feedstock",
volume = "17",
number = "11",
pages = "e0277365",
doi = "10.1371/journal.pone.0277365"
}

Ilić, M., Haegel, F., Lolić, A., Nedić, Z., Tosti, T., Ignjatović, I. S., Sredović Ignjatović, I., Linden, A., Jablonowski, N. D.,& Hartmann, H.. (2022). Surface functional groups and degree of carbonization of selected chars from different processes and feedstock. in PLoS ONE
Public Library of Science., 17(11), e0277365.
https://doi.org/10.1371/journal.pone.0277365

Ilić M, Haegel F, Lolić A, Nedić Z, Tosti T, Ignjatović IS, Sredović Ignjatović I, Linden A, Jablonowski ND, Hartmann H. Surface functional groups and degree of carbonization of selected chars from different processes and feedstock. in PLoS ONE. 2022;17(11):e0277365.
doi:10.1371/journal.pone.0277365 .

Ilić, Marija, Haegel, Franz-Hubert, Lolić, Aleksandar, Nedić, Zoran, Tosti, Tomislav, Ignjatović, Ivana Sredović, Sredović Ignjatović, Ivana, Linden, Andreas, Jablonowski, Nicolai D., Hartmann, Heinrich, "Surface functional groups and degree of carbonization of selected chars from different processes and feedstock" in PLoS ONE, 17, no. 11 (2022):e0277365,
https://doi.org/10.1371/journal.pone.0277365 . .

TY  - JOUR
AU  - Vrhovac, Lidija
AU  - Šelemetjev, Sonja A.
AU  - Vatić, Saša
AU  - Mitrović, Aleksandar
AU  - Milošević, Jelica
AU  - Lolić, Aleksandar
AU  - Beletić, Anđelo D.
AU  - Polović, Natalija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4295
AB  - Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778, whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum.
PB  - Elsevier
T2  - Talanta
T1  - Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors
VL  - 223
SP  - 121588
DO  - 10.1016/j.talanta.2020.121588
ER  - 

@article{
author = "Vrhovac, Lidija and Šelemetjev, Sonja A. and Vatić, Saša and Mitrović, Aleksandar and Milošević, Jelica and Lolić, Aleksandar and Beletić, Anđelo D. and Polović, Natalija",
year = "2021",
abstract = "Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778, whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum.",
publisher = "Elsevier",
journal = "Talanta",
title = "Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors",
volume = "223",
pages = "121588",
doi = "10.1016/j.talanta.2020.121588"
}

Vrhovac, L., Šelemetjev, S. A., Vatić, S., Mitrović, A., Milošević, J., Lolić, A., Beletić, A. D.,& Polović, N.. (2021). Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors. in Talanta
Elsevier., 223, 121588.
https://doi.org/10.1016/j.talanta.2020.121588

Vrhovac L, Šelemetjev SA, Vatić S, Mitrović A, Milošević J, Lolić A, Beletić AD, Polović N. Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors. in Talanta. 2021;223:121588.
doi:10.1016/j.talanta.2020.121588 .

Vrhovac, Lidija, Šelemetjev, Sonja A., Vatić, Saša, Mitrović, Aleksandar, Milošević, Jelica, Lolić, Aleksandar, Beletić, Anđelo D., Polović, Natalija, "Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors" in Talanta, 223 (2021):121588,
https://doi.org/10.1016/j.talanta.2020.121588 . .

TY  - JOUR
AU  - Stevanović, Nikola R.
AU  - Apostolović, Danijela
AU  - Milčić, Miloš K.
AU  - Lolić, Aleksandar
AU  - Hage, Marianne van
AU  - Ćirković-Veličković, Tanja
AU  - Baošić, Rada
PY  - 2021
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/nj/d1nj00040c
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4399
AB  - Different Schiff base complexes have biological activities that make them suitable for drug design. The biological properties of tetradentate Schiff base copper(II) complexed with N,N′-bis(acetylacetone)propylenediimine have been studied. The cytotoxic activity towards Caco-2 cells were determined by MTT, Anexin V and PI apoptosis assays. N,N′-bis(acetylacetone)propylenediimine-copper(II) showed the anti-cancer and anti-proliferative properties by inducing apoptosis in Caco-2 cells. A comparison of the cytotoxic activity of this compound with cisplatin shows that it is more effective on the colorectal cancer cell line Caco-2. The binding capacity and interaction of N,N′-bis(acetylacetone)propylenediimine-copper(II) with HSA were systemically investigated by in vitro fluorescence spectroscopy, CD spectroscopy, and in silico molecular docking study. Furthermore, in vitro and in silico interaction studies indicated that the complex binds to HSA through a static quenching mechanism without changes in protein conformation. The calculated number of binding sites was in line with molecular docking studies. The obtained Ka value suggests that the compound can be released from the protein in target cells. The tetradentate Schiff base copper(II) complex exhibited in vitro biological activities against cancer epithelial cells, which depend on the molecular structure of the complex, causing apoptosis, and the complex can bind to the protein drug carrier in circulation to the target tissue.
T2  - New Journal of Chemistry
T1  - Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2
VL  - 45
IS  - 14
SP  - 6231
EP  - 6237
DO  - 10.1039/D1NJ00040C
ER  - 

@article{
author = "Stevanović, Nikola R. and Apostolović, Danijela and Milčić, Miloš K. and Lolić, Aleksandar and Hage, Marianne van and Ćirković-Veličković, Tanja and Baošić, Rada",
year = "2021",
abstract = "Different Schiff base complexes have biological activities that make them suitable for drug design. The biological properties of tetradentate Schiff base copper(II) complexed with N,N′-bis(acetylacetone)propylenediimine have been studied. The cytotoxic activity towards Caco-2 cells were determined by MTT, Anexin V and PI apoptosis assays. N,N′-bis(acetylacetone)propylenediimine-copper(II) showed the anti-cancer and anti-proliferative properties by inducing apoptosis in Caco-2 cells. A comparison of the cytotoxic activity of this compound with cisplatin shows that it is more effective on the colorectal cancer cell line Caco-2. The binding capacity and interaction of N,N′-bis(acetylacetone)propylenediimine-copper(II) with HSA were systemically investigated by in vitro fluorescence spectroscopy, CD spectroscopy, and in silico molecular docking study. Furthermore, in vitro and in silico interaction studies indicated that the complex binds to HSA through a static quenching mechanism without changes in protein conformation. The calculated number of binding sites was in line with molecular docking studies. The obtained Ka value suggests that the compound can be released from the protein in target cells. The tetradentate Schiff base copper(II) complex exhibited in vitro biological activities against cancer epithelial cells, which depend on the molecular structure of the complex, causing apoptosis, and the complex can bind to the protein drug carrier in circulation to the target tissue.",
journal = "New Journal of Chemistry",
title = "Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2",
volume = "45",
number = "14",
pages = "6231-6237",
doi = "10.1039/D1NJ00040C"
}

Stevanović, N. R., Apostolović, D., Milčić, M. K., Lolić, A., Hage, M. v., Ćirković-Veličković, T.,& Baošić, R.. (2021). Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2. in New Journal of Chemistry, 45(14), 6231-6237.
https://doi.org/10.1039/D1NJ00040C

Stevanović NR, Apostolović D, Milčić MK, Lolić A, Hage MV, Ćirković-Veličković T, Baošić R. Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2. in New Journal of Chemistry. 2021;45(14):6231-6237.
doi:10.1039/D1NJ00040C .

Stevanović, Nikola R., Apostolović, Danijela, Milčić, Miloš K., Lolić, Aleksandar, Hage, Marianne van, Ćirković-Veličković, Tanja, Baošić, Rada, "Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2" in New Journal of Chemistry, 45, no. 14 (2021):6231-6237,
https://doi.org/10.1039/D1NJ00040C . .

TY  - DATA
AU  - Stevanović, Nikola R.
AU  - Apostolović, Danijela
AU  - Milčić, Miloš K.
AU  - Lolić, Aleksandar
AU  - Hage, Marianne van
AU  - Ćirković-Veličković, Tanja
AU  - Baošić, Rada
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4400
T2  - New Journal of Chemistry
T1  - Supplementary data for the article: Stevanović, N.; Apostolović, D.; Milčić, M.; Lolić, A.; Hage, M. van; Veličković, T. Ć.; Baošić, R. Interaction, Binding Capacity and Anticancer Properties of N,N′-Bis(Acetylacetone)-Propylenediimine-Copper(II) on Colorectal Cancer Cell Line Caco-2. New J. Chem. 2021, 45 (14), 6231–6237. https://doi.org/10.1039/D1NJ00040C.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4400
ER  - 

@misc{
author = "Stevanović, Nikola R. and Apostolović, Danijela and Milčić, Miloš K. and Lolić, Aleksandar and Hage, Marianne van and Ćirković-Veličković, Tanja and Baošić, Rada",
year = "2021",
journal = "New Journal of Chemistry",
title = "Supplementary data for the article: Stevanović, N.; Apostolović, D.; Milčić, M.; Lolić, A.; Hage, M. van; Veličković, T. Ć.; Baošić, R. Interaction, Binding Capacity and Anticancer Properties of N,N′-Bis(Acetylacetone)-Propylenediimine-Copper(II) on Colorectal Cancer Cell Line Caco-2. New J. Chem. 2021, 45 (14), 6231–6237. https://doi.org/10.1039/D1NJ00040C.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4400"
}

Stevanović, N. R., Apostolović, D., Milčić, M. K., Lolić, A., Hage, M. v., Ćirković-Veličković, T.,& Baošić, R.. (2021). Supplementary data for the article: Stevanović, N.; Apostolović, D.; Milčić, M.; Lolić, A.; Hage, M. van; Veličković, T. Ć.; Baošić, R. Interaction, Binding Capacity and Anticancer Properties of N,N′-Bis(Acetylacetone)-Propylenediimine-Copper(II) on Colorectal Cancer Cell Line Caco-2. New J. Chem. 2021, 45 (14), 6231–6237. https://doi.org/10.1039/D1NJ00040C.. in New Journal of Chemistry.
https://hdl.handle.net/21.15107/rcub_cherry_4400

Stevanović NR, Apostolović D, Milčić MK, Lolić A, Hage MV, Ćirković-Veličković T, Baošić R. Supplementary data for the article: Stevanović, N.; Apostolović, D.; Milčić, M.; Lolić, A.; Hage, M. van; Veličković, T. Ć.; Baošić, R. Interaction, Binding Capacity and Anticancer Properties of N,N′-Bis(Acetylacetone)-Propylenediimine-Copper(II) on Colorectal Cancer Cell Line Caco-2. New J. Chem. 2021, 45 (14), 6231–6237. https://doi.org/10.1039/D1NJ00040C.. in New Journal of Chemistry. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4400 .

Stevanović, Nikola R., Apostolović, Danijela, Milčić, Miloš K., Lolić, Aleksandar, Hage, Marianne van, Ćirković-Veličković, Tanja, Baošić, Rada, "Supplementary data for the article: Stevanović, N.; Apostolović, D.; Milčić, M.; Lolić, A.; Hage, M. van; Veličković, T. Ć.; Baošić, R. Interaction, Binding Capacity and Anticancer Properties of N,N′-Bis(Acetylacetone)-Propylenediimine-Copper(II) on Colorectal Cancer Cell Line Caco-2. New J. Chem. 2021, 45 (14), 6231–6237. https://doi.org/10.1039/D1NJ00040C." in New Journal of Chemistry (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4400 .

TY  - JOUR
AU  - Antunović, Vesna R.
AU  - Baošić, Rada
AU  - Lolić, Aleksandar
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4401
AB  - Background: Diazepam belongs to the group of 1,4-benzodiapines. It is used for the treatment of anxiety, convulsions and as a muscle relaxant. The presence of 4,5-azomethine group enables its electrochemical detection.

Introduction: A screen-printed electrode modified with antimony film was used for the determination of diazepam in pharmaceutical preparations

Methods: Electrode modification was done by ex-situ deposition of antimony on commercially available screen-printed electrode. Parameters affecting the electroanalytical response of the sensor, such as deposition potential, deposition time, and antimony concentration, were examined and optimized. The modified electrode showed enhanced electroactivity for diazepam reduction compared to unmodified electrode. Under optimal conditions, linear sweep voltammetry was used for the determination of analyte.

Results: The sensor showed linear dependence in the range from 0.5 to 10 μmol/L, the correlation coefficient was 0.9992. The limit of detection was 0.33 μmol/L, corresponding limit of quantification was 1.08 μmol/L. Modification enabled determination of diazepam in the presence of oxygen. 

Conclusion: The modified electrode was used for the determination of diazepam in tablets. Results confirmed the applicability of the electrochemical sensor.
PB  - Bentham Science
T2  - Current Pharmaceutical Analysis
T1  - Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations
VL  - 17
IS  - 7
SP  - 945
EP  - 950
DO  - 10.2174/1573412916999200730234925
ER  - 

@article{
author = "Antunović, Vesna R. and Baošić, Rada and Lolić, Aleksandar",
year = "2021",
abstract = "Background: Diazepam belongs to the group of 1,4-benzodiapines. It is used for the treatment of anxiety, convulsions and as a muscle relaxant. The presence of 4,5-azomethine group enables its electrochemical detection.

Introduction: A screen-printed electrode modified with antimony film was used for the determination of diazepam in pharmaceutical preparations

Methods: Electrode modification was done by ex-situ deposition of antimony on commercially available screen-printed electrode. Parameters affecting the electroanalytical response of the sensor, such as deposition potential, deposition time, and antimony concentration, were examined and optimized. The modified electrode showed enhanced electroactivity for diazepam reduction compared to unmodified electrode. Under optimal conditions, linear sweep voltammetry was used for the determination of analyte.

Results: The sensor showed linear dependence in the range from 0.5 to 10 μmol/L, the correlation coefficient was 0.9992. The limit of detection was 0.33 μmol/L, corresponding limit of quantification was 1.08 μmol/L. Modification enabled determination of diazepam in the presence of oxygen. 

Conclusion: The modified electrode was used for the determination of diazepam in tablets. Results confirmed the applicability of the electrochemical sensor.",
publisher = "Bentham Science",
journal = "Current Pharmaceutical Analysis",
title = "Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations",
volume = "17",
number = "7",
pages = "945-950",
doi = "10.2174/1573412916999200730234925"
}

Antunović, V. R., Baošić, R.,& Lolić, A.. (2021). Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations. in Current Pharmaceutical Analysis
Bentham Science., 17(7), 945-950.
https://doi.org/10.2174/1573412916999200730234925

Antunović VR, Baošić R, Lolić A. Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations. in Current Pharmaceutical Analysis. 2021;17(7):945-950.
doi:10.2174/1573412916999200730234925 .

Antunović, Vesna R., Baošić, Rada, Lolić, Aleksandar, "Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations" in Current Pharmaceutical Analysis, 17, no. 7 (2021):945-950,
https://doi.org/10.2174/1573412916999200730234925 . .

TY  - JOUR
AU  - Antunović, Vesna R.
AU  - Baošić, Rada
AU  - Lolić, Aleksandar
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4401
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4402
AB  - Background: Diazepam belongs to the group of 1,4-benzodiapines. It is used for the treatment of anxiety, convulsions and as a muscle relaxant. The presence of 4,5-azomethine group enables its electrochemical detection.Introduction: A screen-printed electrode modified with antimony film was used for the determination of diazepam in pharmaceutical preparationsMethods: Electrode modification was done by ex-situ deposition of antimony on commercially available screen-printed electrode. Parameters affecting the electroanalytical response of the sensor, such as deposition potential, deposition time, and antimony concentration, were examined and optimized. The modified electrode showed enhanced electroactivity for diazepam reduction compared to unmodified electrode. Under optimal conditions, linear sweep voltammetry was used for the determination of analyte.Results: The sensor showed linear dependence in the range from 0.5 to 10 μmol/L, the correlation coefficient was 0.9992. The limit of detection was 0.33 μmol/L, corresponding limit of quantification was 1.08 μmol/L. Modification enabled determination of diazepam in the presence of oxygen. Conclusion: The modified electrode was used for the determination of diazepam in tablets. Results confirmed the applicability of the electrochemical sensor.
PB  - Bentham Science
T2  - Current Pharmaceutical Analysis
T1  - Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations
VL  - 17
IS  - 7
SP  - 945
EP  - 950
DO  - 10.2174/1573412916999200730234925
ER  - 

@article{
author = "Antunović, Vesna R. and Baošić, Rada and Lolić, Aleksandar",
year = "2021",
abstract = "Background: Diazepam belongs to the group of 1,4-benzodiapines. It is used for the treatment of anxiety, convulsions and as a muscle relaxant. The presence of 4,5-azomethine group enables its electrochemical detection.Introduction: A screen-printed electrode modified with antimony film was used for the determination of diazepam in pharmaceutical preparationsMethods: Electrode modification was done by ex-situ deposition of antimony on commercially available screen-printed electrode. Parameters affecting the electroanalytical response of the sensor, such as deposition potential, deposition time, and antimony concentration, were examined and optimized. The modified electrode showed enhanced electroactivity for diazepam reduction compared to unmodified electrode. Under optimal conditions, linear sweep voltammetry was used for the determination of analyte.Results: The sensor showed linear dependence in the range from 0.5 to 10 μmol/L, the correlation coefficient was 0.9992. The limit of detection was 0.33 μmol/L, corresponding limit of quantification was 1.08 μmol/L. Modification enabled determination of diazepam in the presence of oxygen. Conclusion: The modified electrode was used for the determination of diazepam in tablets. Results confirmed the applicability of the electrochemical sensor.",
publisher = "Bentham Science",
journal = "Current Pharmaceutical Analysis",
title = "Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations",
volume = "17",
number = "7",
pages = "945-950",
doi = "10.2174/1573412916999200730234925"
}

Antunović, V. R., Baošić, R.,& Lolić, A.. (2021). Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations. in Current Pharmaceutical Analysis
Bentham Science., 17(7), 945-950.
https://doi.org/10.2174/1573412916999200730234925

Antunović VR, Baošić R, Lolić A. Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations. in Current Pharmaceutical Analysis. 2021;17(7):945-950.
doi:10.2174/1573412916999200730234925 .

Antunović, Vesna R., Baošić, Rada, Lolić, Aleksandar, "Voltammetric determination of diazepam on antimony film screen-printed electrode in pharmaceutical formulations" in Current Pharmaceutical Analysis, 17, no. 7 (2021):945-950,
https://doi.org/10.2174/1573412916999200730234925 . .

TY  - JOUR
AU  - Kecojević, Isidora
AU  - Đekić, Sanja
AU  - Lazović, Milana
AU  - Mrkajić, Danica
AU  - Baošić, Rada
AU  - Lolić, Aleksandar
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0956713520306095
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4433
AB  - A method using modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) without clean-up step based on the sample dilution approach followed by the liquid chromatography coupled with the tandem mass spectrometry (LC-MS/MS) has been established for determination of 179 pesticide residues in cabbage and rice samples. Two different LC columns were used and their sensitivity was investigated regarding the sample volume. Evaluation of the matrix effect in context of sample dilution and applied extraction solvents was investigated. The method was validated according to Document SANTE/12682/2019. For cabbage samples, recoveries were 70–120% for concentration level of 5 μg/kg; and 70–115% for concentration level of 50 μg/kg; precision RSDr <17%; limits of detection and quantification were in the ranges of 0.4–5.5 and 1.2–16.7 μg/kg, respectively. For rice samples, recoveries were 70–109% for 10 μg/kg concentration; 70–115% for 100 μg/kg concentration; precision RSDr <14%, while limits of detection and quantification were in the ranges of 0.5–4.2 and 1.5–12.7 μg/kg, respectively.
PB  - Elsevier
T2  - Food Control
T1  - Evaluation of LC-MS/MS methodology for determination of 179 multi-class pesticides in cabbage and rice by modified QuEChERS extraction
VL  - 123
SP  - 107693
DO  - 10.1016/j.foodcont.2020.107693
ER  - 

@article{
author = "Kecojević, Isidora and Đekić, Sanja and Lazović, Milana and Mrkajić, Danica and Baošić, Rada and Lolić, Aleksandar",
year = "2021",
abstract = "A method using modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) without clean-up step based on the sample dilution approach followed by the liquid chromatography coupled with the tandem mass spectrometry (LC-MS/MS) has been established for determination of 179 pesticide residues in cabbage and rice samples. Two different LC columns were used and their sensitivity was investigated regarding the sample volume. Evaluation of the matrix effect in context of sample dilution and applied extraction solvents was investigated. The method was validated according to Document SANTE/12682/2019. For cabbage samples, recoveries were 70–120% for concentration level of 5 μg/kg; and 70–115% for concentration level of 50 μg/kg; precision RSDr <17%; limits of detection and quantification were in the ranges of 0.4–5.5 and 1.2–16.7 μg/kg, respectively. For rice samples, recoveries were 70–109% for 10 μg/kg concentration; 70–115% for 100 μg/kg concentration; precision RSDr <14%, while limits of detection and quantification were in the ranges of 0.5–4.2 and 1.5–12.7 μg/kg, respectively.",
publisher = "Elsevier",
journal = "Food Control",
title = "Evaluation of LC-MS/MS methodology for determination of 179 multi-class pesticides in cabbage and rice by modified QuEChERS extraction",
volume = "123",
pages = "107693",
doi = "10.1016/j.foodcont.2020.107693"
}

Kecojević, I., Đekić, S., Lazović, M., Mrkajić, D., Baošić, R.,& Lolić, A.. (2021). Evaluation of LC-MS/MS methodology for determination of 179 multi-class pesticides in cabbage and rice by modified QuEChERS extraction. in Food Control
Elsevier., 123, 107693.
https://doi.org/10.1016/j.foodcont.2020.107693

Kecojević I, Đekić S, Lazović M, Mrkajić D, Baošić R, Lolić A. Evaluation of LC-MS/MS methodology for determination of 179 multi-class pesticides in cabbage and rice by modified QuEChERS extraction. in Food Control. 2021;123:107693.
doi:10.1016/j.foodcont.2020.107693 .

Kecojević, Isidora, Đekić, Sanja, Lazović, Milana, Mrkajić, Danica, Baošić, Rada, Lolić, Aleksandar, "Evaluation of LC-MS/MS methodology for determination of 179 multi-class pesticides in cabbage and rice by modified QuEChERS extraction" in Food Control, 123 (2021):107693,
https://doi.org/10.1016/j.foodcont.2020.107693 . .

TY  - DATA
AU  - Kecojević, Isidora
AU  - Đekić, Sanja
AU  - Lazović, Milana
AU  - Mrkajić, Danica
AU  - Baošić, Rada
AU  - Lolić, Aleksandar
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4434
PB  - Elsevier
T2  - Food Control
T1  - Supplementary data for the article: Kecojević, I.; Đekić, S.; Lazović, M.; Mrkajić, D.; Baošić, R.; Lolić, A. Evaluation of LC-MS/MS Methodology for Determination of 179 Multi-Class Pesticides in Cabbage and Rice by Modified QuEChERS Extraction. Food Control 2021, 123, 107693. https://doi.org/10.1016/j.foodcont.2020.107693.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4434
ER  - 

@misc{
author = "Kecojević, Isidora and Đekić, Sanja and Lazović, Milana and Mrkajić, Danica and Baošić, Rada and Lolić, Aleksandar",
year = "2021",
publisher = "Elsevier",
journal = "Food Control",
title = "Supplementary data for the article: Kecojević, I.; Đekić, S.; Lazović, M.; Mrkajić, D.; Baošić, R.; Lolić, A. Evaluation of LC-MS/MS Methodology for Determination of 179 Multi-Class Pesticides in Cabbage and Rice by Modified QuEChERS Extraction. Food Control 2021, 123, 107693. https://doi.org/10.1016/j.foodcont.2020.107693.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4434"
}

Kecojević, I., Đekić, S., Lazović, M., Mrkajić, D., Baošić, R.,& Lolić, A.. (2021). Supplementary data for the article: Kecojević, I.; Đekić, S.; Lazović, M.; Mrkajić, D.; Baošić, R.; Lolić, A. Evaluation of LC-MS/MS Methodology for Determination of 179 Multi-Class Pesticides in Cabbage and Rice by Modified QuEChERS Extraction. Food Control 2021, 123, 107693. https://doi.org/10.1016/j.foodcont.2020.107693.. in Food Control
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4434

Kecojević I, Đekić S, Lazović M, Mrkajić D, Baošić R, Lolić A. Supplementary data for the article: Kecojević, I.; Đekić, S.; Lazović, M.; Mrkajić, D.; Baošić, R.; Lolić, A. Evaluation of LC-MS/MS Methodology for Determination of 179 Multi-Class Pesticides in Cabbage and Rice by Modified QuEChERS Extraction. Food Control 2021, 123, 107693. https://doi.org/10.1016/j.foodcont.2020.107693.. in Food Control. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4434 .

Kecojević, Isidora, Đekić, Sanja, Lazović, Milana, Mrkajić, Danica, Baošić, Rada, Lolić, Aleksandar, "Supplementary data for the article: Kecojević, I.; Đekić, S.; Lazović, M.; Mrkajić, D.; Baošić, R.; Lolić, A. Evaluation of LC-MS/MS Methodology for Determination of 179 Multi-Class Pesticides in Cabbage and Rice by Modified QuEChERS Extraction. Food Control 2021, 123, 107693. https://doi.org/10.1016/j.foodcont.2020.107693." in Food Control (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4434 .

TY  - JOUR
AU  - Marković, Srđan
AU  - Milošević, Jelica
AU  - Đurić, Milica
AU  - Lolić, Aleksandar
AU  - Polović, Natalija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4479
AB  - Papain is a proteolytic enzyme of great commercial value. It is a cysteine protease highly expressed in Carica papaya fruit latex, but also present in papaya leaves. Purification procedures mostly deal with the latex and include a combination of precipitation and/or chromatographic techniques. Due to its solubility, structure and activity characteristics, the pH and salt content play significant roles in handling papain extracts. Here we report a simple, rapid and easily scalable procedure for papain purification from papaya leaves, which contain different contaminants as compared to papaya latex. Sodium chloride precipitation of contaminants at pH 5 followed by ammonium sulphate precipitation resulted in the removal of other leaf proteins and protein fragments from papain solution and about a 3-fold purification. The procedure also benefits from the suppression of autoproteolysis and preservation of the native structure, as confirmed by FTIR analysis, and the high recovery of activity of over 80%.
T2  - Archives of Biological Sciences
T1  - One-step purification and freeze stability of papain at acidic pH values
VL  - 73
IS  - 1
SP  - 57
EP  - 64
DO  - 10.2298/ABS201217001M
ER  - 

@article{
author = "Marković, Srđan and Milošević, Jelica and Đurić, Milica and Lolić, Aleksandar and Polović, Natalija",
year = "2021",
abstract = "Papain is a proteolytic enzyme of great commercial value. It is a cysteine protease highly expressed in Carica papaya fruit latex, but also present in papaya leaves. Purification procedures mostly deal with the latex and include a combination of precipitation and/or chromatographic techniques. Due to its solubility, structure and activity characteristics, the pH and salt content play significant roles in handling papain extracts. Here we report a simple, rapid and easily scalable procedure for papain purification from papaya leaves, which contain different contaminants as compared to papaya latex. Sodium chloride precipitation of contaminants at pH 5 followed by ammonium sulphate precipitation resulted in the removal of other leaf proteins and protein fragments from papain solution and about a 3-fold purification. The procedure also benefits from the suppression of autoproteolysis and preservation of the native structure, as confirmed by FTIR analysis, and the high recovery of activity of over 80%.",
journal = "Archives of Biological Sciences",
title = "One-step purification and freeze stability of papain at acidic pH values",
volume = "73",
number = "1",
pages = "57-64",
doi = "10.2298/ABS201217001M"
}

Marković, S., Milošević, J., Đurić, M., Lolić, A.,& Polović, N.. (2021). One-step purification and freeze stability of papain at acidic pH values. in Archives of Biological Sciences, 73(1), 57-64.
https://doi.org/10.2298/ABS201217001M

Marković S, Milošević J, Đurić M, Lolić A, Polović N. One-step purification and freeze stability of papain at acidic pH values. in Archives of Biological Sciences. 2021;73(1):57-64.
doi:10.2298/ABS201217001M .

Marković, Srđan, Milošević, Jelica, Đurić, Milica, Lolić, Aleksandar, Polović, Natalija, "One-step purification and freeze stability of papain at acidic pH values" in Archives of Biological Sciences, 73, no. 1 (2021):57-64,
https://doi.org/10.2298/ABS201217001M . .

TY  - JOUR
AU  - Stevanović, Nikola R.
AU  - Apostolović, Danijela
AU  - Milčić, Miloš K.
AU  - Lolić, Aleksandar
AU  - Hage, Marianne van
AU  - Ćirković-Veličković, Tanja
AU  - Baošić, Rada
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4856
AB  - Different Schiff base complexes have biological activities that make them suitable for drug design. The biological properties of tetradentate Schiff base copper(II) complexed with N,N′-bis(acetylacetone)propylenediimine have been studied. The cytotoxic activity towards Caco-2 cells were determined by MTT, Anexin V and PI apoptosis assays. N,N′-bis(acetylacetone)propylenediimine-copper(II) showed the anti-cancer and anti-proliferative properties by inducing apoptosis in Caco-2 cells. A comparison of the cytotoxic activity of this compound with cisplatin shows that it is more effective on the colorectal cancer cell line Caco-2. The binding capacity and interaction of N,N′-bis(acetylacetone)propylenediimine-copper(II) with HSA were systemically investigated by in vitro fluorescence spectroscopy, CD spectroscopy, and in silico molecular docking study. Furthermore, in vitro and in silico interaction studies indicated that the complex binds to HSA through a static quenching mechanism without changes in protein conformation. The calculated number of binding sites was in line with molecular docking studies. The obtained Ka value suggests that the compound can be released from the protein in target cells. The tetradentate Schiff base copper(II) complex exhibited in vitro biological activities against cancer epithelial cells, which depend on the molecular structure of the complex, causing apoptosis, and the complex can bind to the protein drug carrier in circulation to the target tissue.
T2  - New Journal of Chemistry
T1  - Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2
VL  - 45
IS  - 14
SP  - 6231
EP  - 6237
DO  - 10.1039/D1NJ00040C
ER  - 

@article{
author = "Stevanović, Nikola R. and Apostolović, Danijela and Milčić, Miloš K. and Lolić, Aleksandar and Hage, Marianne van and Ćirković-Veličković, Tanja and Baošić, Rada",
year = "2021",
abstract = "Different Schiff base complexes have biological activities that make them suitable for drug design. The biological properties of tetradentate Schiff base copper(II) complexed with N,N′-bis(acetylacetone)propylenediimine have been studied. The cytotoxic activity towards Caco-2 cells were determined by MTT, Anexin V and PI apoptosis assays. N,N′-bis(acetylacetone)propylenediimine-copper(II) showed the anti-cancer and anti-proliferative properties by inducing apoptosis in Caco-2 cells. A comparison of the cytotoxic activity of this compound with cisplatin shows that it is more effective on the colorectal cancer cell line Caco-2. The binding capacity and interaction of N,N′-bis(acetylacetone)propylenediimine-copper(II) with HSA were systemically investigated by in vitro fluorescence spectroscopy, CD spectroscopy, and in silico molecular docking study. Furthermore, in vitro and in silico interaction studies indicated that the complex binds to HSA through a static quenching mechanism without changes in protein conformation. The calculated number of binding sites was in line with molecular docking studies. The obtained Ka value suggests that the compound can be released from the protein in target cells. The tetradentate Schiff base copper(II) complex exhibited in vitro biological activities against cancer epithelial cells, which depend on the molecular structure of the complex, causing apoptosis, and the complex can bind to the protein drug carrier in circulation to the target tissue.",
journal = "New Journal of Chemistry",
title = "Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2",
volume = "45",
number = "14",
pages = "6231-6237",
doi = "10.1039/D1NJ00040C"
}

Stevanović, N. R., Apostolović, D., Milčić, M. K., Lolić, A., Hage, M. v., Ćirković-Veličković, T.,& Baošić, R.. (2021). Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2. in New Journal of Chemistry, 45(14), 6231-6237.
https://doi.org/10.1039/D1NJ00040C

Stevanović NR, Apostolović D, Milčić MK, Lolić A, Hage MV, Ćirković-Veličković T, Baošić R. Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2. in New Journal of Chemistry. 2021;45(14):6231-6237.
doi:10.1039/D1NJ00040C .

Stevanović, Nikola R., Apostolović, Danijela, Milčić, Miloš K., Lolić, Aleksandar, Hage, Marianne van, Ćirković-Veličković, Tanja, Baošić, Rada, "Interaction, binding capacity and anticancer properties of N,N′-bis(acetylacetone)-propylenediimine-copper(II) on colorectal cancer cell line Caco-2" in New Journal of Chemistry, 45, no. 14 (2021):6231-6237,
https://doi.org/10.1039/D1NJ00040C . .

TY  - JOUR
AU  - Antunović, Vesna R.
AU  - Tripković, Tatjana
AU  - Tomašević, Biljana
AU  - Baošić, Rada
AU  - Jelić, Dijana
AU  - Lolić, Aleksandar
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4985
AB  - This paper describes the determination of Pb and Cu with a Nafion-modified glassy carbon electrode and MnCo2O4 nanoparticles as working electrode for anodic stripping voltammetry. Pb and Cu were accumulated in HCl/KCl (0.1 mol dm-3) at a potential of -1.4 V (vs. Ag/AgCl electrode) for 480 s, followed by a linear sweep anodic stripping voltammetry (ASV) scan from -1.0 to +0.5 V. Under optimum conditions, the calibration curves were linear in the range of 0.01 - 8 and 0.01 - 5 mg dm-3 for Pb and Cu, respectively. Effect of sample dilution, accumulation time and potential were optimized. A study of interfering substances was performed. A significant increase in current was obtained at the modified electrode in comparison with the bare glassy carbon electrode. The modified electrode was successfully applied for determination of Pb and Cu in wine samples after a simple preparation procedure. Pb and Cu content in wine was used for estimation of the target hazard quotient (THQ) values for minimal and maximal levels of the metals.
PB  - Japan Society for Analytical Chemistry
T2  - Analytical Sciences
T1  - Voltammetric determination of lead and copper in wine by modified glassy carbon electrode
VL  - 37
IS  - 2
SP  - 353
DO  - 10.2116/analsci.20P302
ER  - 

@article{
author = "Antunović, Vesna R. and Tripković, Tatjana and Tomašević, Biljana and Baošić, Rada and Jelić, Dijana and Lolić, Aleksandar",
year = "2021",
abstract = "This paper describes the determination of Pb and Cu with a Nafion-modified glassy carbon electrode and MnCo2O4 nanoparticles as working electrode for anodic stripping voltammetry. Pb and Cu were accumulated in HCl/KCl (0.1 mol dm-3) at a potential of -1.4 V (vs. Ag/AgCl electrode) for 480 s, followed by a linear sweep anodic stripping voltammetry (ASV) scan from -1.0 to +0.5 V. Under optimum conditions, the calibration curves were linear in the range of 0.01 - 8 and 0.01 - 5 mg dm-3 for Pb and Cu, respectively. Effect of sample dilution, accumulation time and potential were optimized. A study of interfering substances was performed. A significant increase in current was obtained at the modified electrode in comparison with the bare glassy carbon electrode. The modified electrode was successfully applied for determination of Pb and Cu in wine samples after a simple preparation procedure. Pb and Cu content in wine was used for estimation of the target hazard quotient (THQ) values for minimal and maximal levels of the metals.",
publisher = "Japan Society for Analytical Chemistry",
journal = "Analytical Sciences",
title = "Voltammetric determination of lead and copper in wine by modified glassy carbon electrode",
volume = "37",
number = "2",
pages = "353",
doi = "10.2116/analsci.20P302"
}

Antunović, V. R., Tripković, T., Tomašević, B., Baošić, R., Jelić, D.,& Lolić, A.. (2021). Voltammetric determination of lead and copper in wine by modified glassy carbon electrode. in Analytical Sciences
Japan Society for Analytical Chemistry., 37(2), 353.
https://doi.org/10.2116/analsci.20P302

Antunović VR, Tripković T, Tomašević B, Baošić R, Jelić D, Lolić A. Voltammetric determination of lead and copper in wine by modified glassy carbon electrode. in Analytical Sciences. 2021;37(2):353.
doi:10.2116/analsci.20P302 .

Antunović, Vesna R., Tripković, Tatjana, Tomašević, Biljana, Baošić, Rada, Jelić, Dijana, Lolić, Aleksandar, "Voltammetric determination of lead and copper in wine by modified glassy carbon electrode" in Analytical Sciences, 37, no. 2 (2021):353,
https://doi.org/10.2116/analsci.20P302 . .

TY  - JOUR
AU  - Đogo Mračević, Svetlana
AU  - Krstić, Marko
AU  - Lolić, Aleksandar
AU  - Ražić, Slavica
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4986
AB  - A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,
phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemical
characteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeys
showed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. The
concentrations of 15 elements in honey samples were determined using inductively coupled plasma optical
emission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,
and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values were
lower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC and
cooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the most
abundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significant
variability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.
The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metals
according to European Commission Regulation. The antioxidant activity was evaluated by DPPH
(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria
(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radical
scavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity was
observed with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96
to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterial
growth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lower
than 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimental
results was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised method
was applied. According to the obtained outcome of applied algorithms, both botanical and geographical origin
have important role in the elemental composition, antioxidant and antimicrobial activities.
PB  - Elsevier
T2  - Microchemical Journal
T1  - Comparative study of the cheical composition and biological potential of honey from different regions of Serbia
VL  - 152
SP  - 104420
DO  - 10.1016/j.microc.2019.104420
ER  - 

@article{
author = "Đogo Mračević, Svetlana and Krstić, Marko and Lolić, Aleksandar and Ražić, Slavica",
year = "2020",
abstract = "A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,
phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemical
characteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeys
showed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. The
concentrations of 15 elements in honey samples were determined using inductively coupled plasma optical
emission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,
and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values were
lower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC and
cooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the most
abundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significant
variability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.
The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metals
according to European Commission Regulation. The antioxidant activity was evaluated by DPPH
(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria
(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radical
scavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity was
observed with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96
to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterial
growth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lower
than 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimental
results was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised method
was applied. According to the obtained outcome of applied algorithms, both botanical and geographical origin
have important role in the elemental composition, antioxidant and antimicrobial activities.",
publisher = "Elsevier",
journal = "Microchemical Journal",
title = "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia",
volume = "152",
pages = "104420",
doi = "10.1016/j.microc.2019.104420"
}

Đogo Mračević, S., Krstić, M., Lolić, A.,& Ražić, S.. (2020). Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal
Elsevier., 152, 104420.
https://doi.org/10.1016/j.microc.2019.104420

Đogo Mračević S, Krstić M, Lolić A, Ražić S. Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal. 2020;152:104420.
doi:10.1016/j.microc.2019.104420 .

Đogo Mračević, Svetlana, Krstić, Marko, Lolić, Aleksandar, Ražić, Slavica, "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia" in Microchemical Journal, 152 (2020):104420,
https://doi.org/10.1016/j.microc.2019.104420 . .

TY  - JOUR
AU  - Marković, Marijana
AU  - Petrović, Jana
AU  - Đogo Mračević, Svetlana
AU  - Lolić, Aleksandar
AU  - Krstić, Marko
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4984
AB  - Uvod: Kofein je prirodni alkaloid, izolovan iz više od 60 biljnih vrsta. Preko 80 % svetske populacije
svakodnevno unosi kofein u organizam kroz konzumaciju kafe, čajeva, čokolade i, u poslednje
vreme, energetskih pića. Ne treba zanemariti činjenicu da kofein, kao farmakološki aktivna
supstanca, ulazi u sastav mnogih lekova koji se na tržištu Republike Srbije izdaju bez lekarskog
recepta. Sem toga, kofein je psihoaktivni stimulans, blag je diuretik i pokazuje interakciju sa nekim
lekovima usled indukcije CYP1 i CYP2 izoenzima, zbog čega je potrebno voditi računa o količini
koja se unese u organizam. U skladu s tim, važno je i da sadržaj kofeina u energetskim pićima odgovara
podacima koje proizvođači navode na deklaraciji.
Cilj: Cilj ovog rada bio je spektrofotometrijsko određivanje sadržaja kofeina u uzorcima energetskih
pića i čajeva proizvođača dostupnih na tržištu R. Srbije.
Materijal i metode: Kofein je određivan metodom ultraljubičaste i vidljive (UV/VIS) spektrofotometrije
u devet vrsta energetskih pića i po četiri uzorka crnog i zelenog čaja.
Rezultati: U uzorcima energetskih pića određeni sadržaj kofeina je u opsegu od 26,81 do 35,44
mg/100 mL i u skladu je s deklarisanim sadržajem. Sadržaj kofeina u uzorcima čajeva varira u
odnosu na vrstu (11,27-22,61 mg/100 mL zeleni i 13,85-22,73 mg/100 mL crni), ali je vezan i za
poreklo, uslove gajenja i proces prerade čajeva.
Zaključak: Ultraljubičasta i vidljiva spektrofotometrija se pokazala kao brza, precizna i jednostavna
metoda za određivanje kofeina u energetskim pićima i čajevima. Imajući u vidu dobijene rezultate,
važno je istaći da unos kofeina kroz uobičajenu dnevnu konzumaciju čajeva i energetskih pića
ne bi trebalo da utiče negativno na zdravlje ljudi. Svakako treba imati u vidu da se ovaj alkaloid
dodatno može uneti i putem drugih izvora, što je posebno važno za određene grupe pacijenata.
AB  - Introduction: Caffeine is a natural alkaloid, isolated from more than 60 plant species. Over 80%  of  the  world’s  population  consumes  caffeine  on  daily  basis  through  the  consumption  of  coffee,  tea,  chocolates  and,  more  recently,  energy  drinks.  As  a  pharmacologically  potent  substance  caffeine  is  an  ingredient  of  many  medicaments  available  on  the  pharmaceutical  market of Republic of Serbia without a doctor’s prescription. Besides that, caffeine acts as a psychoactive  stimulant,  mild  diuretic,  and  displays  an  interaction  with  some  medicaments  due  to  the  induction  of  CYP1  and  CYP2  isoenzymes.  Keeping  in  mind  all  of  the  above,  it  is necessary to take into account the daily caffeine intake, as well as to control its content in energy drinks.Aim:  The  aim  of  this  study  was  spectrophotometric  determination  of  caffeine  content  in  energy drinks and tea, available in markets of the Republic of Serbia. Material  and  Methods:  Caffeine  was  determinated  by  UV/VIS  spectrophotometry  in  nine  types of energy drinks and eight samples of black and green tea. Results: The results showed that caffeine content in energy drink samples ranged from 26.81 to 35.44 mg/100mL and corresponds to declaration. The caffeine content in tea samples varies with the species (11.27-22.61 mg/100mL for green and 13.85-22.73 mg/100mL for black tea), but also with origin, growth conditions and tea processing.Conclusion: The obtained data suggest that UV/VIS spectrophotometry can be used for ro-utine determination and control of caffeine content in energy drinks and tea. It is important to  note  that  caffeine  intake  through  the  usual  daily  consumption  of  tea  and  energy  drinks  should not have a negative impact on human health, but this alkaloid could be additionally consumed through other sources, which is especially important for certain groups of patients.
PB  - Beograd : Medicinski fakultet
T2  - Medicinski podmladak
T1  - Spectrophotometric determination of caffeine in energy drinks and teas
T1  - Spektrofotometrijsko određivanje sadržaja kofeina  u energetskim pićima i čajevima
VL  - 71
IS  - 3
SP  - 8
DO  - 10.5937/mp71-26638
ER  - 

@article{
author = "Marković, Marijana and Petrović, Jana and Đogo Mračević, Svetlana and Lolić, Aleksandar and Krstić, Marko",
year = "2020",
abstract = "Uvod: Kofein je prirodni alkaloid, izolovan iz više od 60 biljnih vrsta. Preko 80 % svetske populacije
svakodnevno unosi kofein u organizam kroz konzumaciju kafe, čajeva, čokolade i, u poslednje
vreme, energetskih pića. Ne treba zanemariti činjenicu da kofein, kao farmakološki aktivna
supstanca, ulazi u sastav mnogih lekova koji se na tržištu Republike Srbije izdaju bez lekarskog
recepta. Sem toga, kofein je psihoaktivni stimulans, blag je diuretik i pokazuje interakciju sa nekim
lekovima usled indukcije CYP1 i CYP2 izoenzima, zbog čega je potrebno voditi računa o količini
koja se unese u organizam. U skladu s tim, važno je i da sadržaj kofeina u energetskim pićima odgovara
podacima koje proizvođači navode na deklaraciji.
Cilj: Cilj ovog rada bio je spektrofotometrijsko određivanje sadržaja kofeina u uzorcima energetskih
pića i čajeva proizvođača dostupnih na tržištu R. Srbije.
Materijal i metode: Kofein je određivan metodom ultraljubičaste i vidljive (UV/VIS) spektrofotometrije
u devet vrsta energetskih pića i po četiri uzorka crnog i zelenog čaja.
Rezultati: U uzorcima energetskih pića određeni sadržaj kofeina je u opsegu od 26,81 do 35,44
mg/100 mL i u skladu je s deklarisanim sadržajem. Sadržaj kofeina u uzorcima čajeva varira u
odnosu na vrstu (11,27-22,61 mg/100 mL zeleni i 13,85-22,73 mg/100 mL crni), ali je vezan i za
poreklo, uslove gajenja i proces prerade čajeva.
Zaključak: Ultraljubičasta i vidljiva spektrofotometrija se pokazala kao brza, precizna i jednostavna
metoda za određivanje kofeina u energetskim pićima i čajevima. Imajući u vidu dobijene rezultate,
važno je istaći da unos kofeina kroz uobičajenu dnevnu konzumaciju čajeva i energetskih pića
ne bi trebalo da utiče negativno na zdravlje ljudi. Svakako treba imati u vidu da se ovaj alkaloid
dodatno može uneti i putem drugih izvora, što je posebno važno za određene grupe pacijenata., Introduction: Caffeine is a natural alkaloid, isolated from more than 60 plant species. Over 80%  of  the  world’s  population  consumes  caffeine  on  daily  basis  through  the  consumption  of  coffee,  tea,  chocolates  and,  more  recently,  energy  drinks.  As  a  pharmacologically  potent  substance  caffeine  is  an  ingredient  of  many  medicaments  available  on  the  pharmaceutical  market of Republic of Serbia without a doctor’s prescription. Besides that, caffeine acts as a psychoactive  stimulant,  mild  diuretic,  and  displays  an  interaction  with  some  medicaments  due  to  the  induction  of  CYP1  and  CYP2  isoenzymes.  Keeping  in  mind  all  of  the  above,  it  is necessary to take into account the daily caffeine intake, as well as to control its content in energy drinks.Aim:  The  aim  of  this  study  was  spectrophotometric  determination  of  caffeine  content  in  energy drinks and tea, available in markets of the Republic of Serbia. Material  and  Methods:  Caffeine  was  determinated  by  UV/VIS  spectrophotometry  in  nine  types of energy drinks and eight samples of black and green tea. Results: The results showed that caffeine content in energy drink samples ranged from 26.81 to 35.44 mg/100mL and corresponds to declaration. The caffeine content in tea samples varies with the species (11.27-22.61 mg/100mL for green and 13.85-22.73 mg/100mL for black tea), but also with origin, growth conditions and tea processing.Conclusion: The obtained data suggest that UV/VIS spectrophotometry can be used for ro-utine determination and control of caffeine content in energy drinks and tea. It is important to  note  that  caffeine  intake  through  the  usual  daily  consumption  of  tea  and  energy  drinks  should not have a negative impact on human health, but this alkaloid could be additionally consumed through other sources, which is especially important for certain groups of patients.",
publisher = "Beograd : Medicinski fakultet",
journal = "Medicinski podmladak",
title = "Spectrophotometric determination of caffeine in energy drinks and teas, Spektrofotometrijsko određivanje sadržaja kofeina  u energetskim pićima i čajevima",
volume = "71",
number = "3",
pages = "8",
doi = "10.5937/mp71-26638"
}

Marković, M., Petrović, J., Đogo Mračević, S., Lolić, A.,& Krstić, M.. (2020). Spectrophotometric determination of caffeine in energy drinks and teas. in Medicinski podmladak
Beograd : Medicinski fakultet., 71(3), 8.
https://doi.org/10.5937/mp71-26638

Marković M, Petrović J, Đogo Mračević S, Lolić A, Krstić M. Spectrophotometric determination of caffeine in energy drinks and teas. in Medicinski podmladak. 2020;71(3):8.
doi:10.5937/mp71-26638 .

Marković, Marijana, Petrović, Jana, Đogo Mračević, Svetlana, Lolić, Aleksandar, Krstić, Marko, "Spectrophotometric determination of caffeine in energy drinks and teas" in Medicinski podmladak, 71, no. 3 (2020):8,
https://doi.org/10.5937/mp71-26638 . .

TY  - JOUR
AU  - Đogo Mračević, Svetlana
AU  - Krstić, Marko
AU  - Lolić, Aleksandar
AU  - Ražić, Slavica
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4995
AB  - A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemicalcharacteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeysshowed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. Theconcentrations of 15 elements in honey samples were determined using inductively coupled plasma opticalemission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values werelower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC andcooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the mostabundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significantvariability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metalsaccording to European Commission Regulation. The antioxidant activity was evaluated by DPPH(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radicalscavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity wasobserved with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterialgrowth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lowerthan 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimentalresults was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised methodwas applied. According to the obtained outcome of applied algorithms, both botanical and geographical originhave important role in the elemental composition, antioxidant and antimicrobial activities.
PB  - Elsevier
T2  - Microchemical Journal
T1  - Comparative study of the cheical composition and biological potential of honey from different regions of Serbia
VL  - 152
SP  - 104420
DO  - 10.1016/j.microc.2019.104420
ER  - 

@article{
author = "Đogo Mračević, Svetlana and Krstić, Marko and Lolić, Aleksandar and Ražić, Slavica",
year = "2020",
abstract = "A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemicalcharacteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeysshowed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. Theconcentrations of 15 elements in honey samples were determined using inductively coupled plasma opticalemission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values werelower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC andcooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the mostabundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significantvariability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metalsaccording to European Commission Regulation. The antioxidant activity was evaluated by DPPH(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radicalscavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity wasobserved with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterialgrowth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lowerthan 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimentalresults was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised methodwas applied. According to the obtained outcome of applied algorithms, both botanical and geographical originhave important role in the elemental composition, antioxidant and antimicrobial activities.",
publisher = "Elsevier",
journal = "Microchemical Journal",
title = "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia",
volume = "152",
pages = "104420",
doi = "10.1016/j.microc.2019.104420"
}

Đogo Mračević, S., Krstić, M., Lolić, A.,& Ražić, S.. (2020). Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal
Elsevier., 152, 104420.
https://doi.org/10.1016/j.microc.2019.104420

Đogo Mračević S, Krstić M, Lolić A, Ražić S. Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal. 2020;152:104420.
doi:10.1016/j.microc.2019.104420 .

Đogo Mračević, Svetlana, Krstić, Marko, Lolić, Aleksandar, Ražić, Slavica, "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia" in Microchemical Journal, 152 (2020):104420,
https://doi.org/10.1016/j.microc.2019.104420 . .