TY  - JOUR
AU  - Ćirković-Veličković, Tanja
AU  - Thunberg, Sarah
AU  - Polović, Natalija
AU  - Neimert-Andersson, Theresa
AU  - Gronlund, Hans
AU  - van Hage, Marianne
AU  - Gafvelin, Guro
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/934
AB  - Background: Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to have both protective and exacerbating effects on the development and maintenance of allergic disease in humans and on markers of allergic inflammation in animal models of allergy. In this study, we investigated the effect of low concentrations of LPS on human peripheral blood mononuclear cells (PBMC) stimulated with the major cat allergen Fel d 1. Methods: Extensive purification of recombinant (r) Fel d 1 yielded essentially endotoxin-free rFel d 1 (0.2 ng LPS/mg protein). PBMCs prepared from 15 subjects having IgE to cat ( gt 0.7 kU(A)/l) and 8 subjects IgE negative to cat were stimulated with 2, 10 or 25 mu g/ml of rFel d 1 in the presence or absence of 50 pg/ml LPS. Proliferation was measured after 7 days of culture and supernatants were analyzed for IFN gamma, IL-5 and IL-10. Results: LPS (50 pg/ml) increased rFel d 1-stimulated proliferation of PBMCs both from subjects IgE-positive and subjects negative to cat allergens. PBMCs from 13 of the subjects did not proliferate in response to stimulation with 2 and 10 mu g/ml rFel d 1 alone but did so in the presence of LPS. Moreover, LPS increased the levels of rFel d 1-stimulated IFN gamma in cultures from cat-negative subjects, IL-5 from cat-positive subjects and IL-10 from both groups. Conclusion: Very low doses of LPS enhance proliferation and decrease the apparent threshold level for cell activation, prompting careful evaluation of allergen stimulated T cell activation in vitro. Copyright (C) 2007 S. Karger AG, Basel.
PB  - Karger, Basel
T2  - International Archives of Allergy and Immunology
T1  - Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells
VL  - 146
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1159/000112497
ER  - 

@article{
author = "Ćirković-Veličković, Tanja and Thunberg, Sarah and Polović, Natalija and Neimert-Andersson, Theresa and Gronlund, Hans and van Hage, Marianne and Gafvelin, Guro",
year = "2008",
abstract = "Background: Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to have both protective and exacerbating effects on the development and maintenance of allergic disease in humans and on markers of allergic inflammation in animal models of allergy. In this study, we investigated the effect of low concentrations of LPS on human peripheral blood mononuclear cells (PBMC) stimulated with the major cat allergen Fel d 1. Methods: Extensive purification of recombinant (r) Fel d 1 yielded essentially endotoxin-free rFel d 1 (0.2 ng LPS/mg protein). PBMCs prepared from 15 subjects having IgE to cat ( gt 0.7 kU(A)/l) and 8 subjects IgE negative to cat were stimulated with 2, 10 or 25 mu g/ml of rFel d 1 in the presence or absence of 50 pg/ml LPS. Proliferation was measured after 7 days of culture and supernatants were analyzed for IFN gamma, IL-5 and IL-10. Results: LPS (50 pg/ml) increased rFel d 1-stimulated proliferation of PBMCs both from subjects IgE-positive and subjects negative to cat allergens. PBMCs from 13 of the subjects did not proliferate in response to stimulation with 2 and 10 mu g/ml rFel d 1 alone but did so in the presence of LPS. Moreover, LPS increased the levels of rFel d 1-stimulated IFN gamma in cultures from cat-negative subjects, IL-5 from cat-positive subjects and IL-10 from both groups. Conclusion: Very low doses of LPS enhance proliferation and decrease the apparent threshold level for cell activation, prompting careful evaluation of allergen stimulated T cell activation in vitro. Copyright (C) 2007 S. Karger AG, Basel.",
publisher = "Karger, Basel",
journal = "International Archives of Allergy and Immunology",
title = "Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells",
volume = "146",
number = "1",
pages = "1-10",
doi = "10.1159/000112497"
}

Ćirković-Veličković, T., Thunberg, S., Polović, N., Neimert-Andersson, T., Gronlund, H., van Hage, M.,& Gafvelin, G.. (2008). Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells. in International Archives of Allergy and Immunology
Karger, Basel., 146(1), 1-10.
https://doi.org/10.1159/000112497

Ćirković-Veličković T, Thunberg S, Polović N, Neimert-Andersson T, Gronlund H, van Hage M, Gafvelin G. Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells. in International Archives of Allergy and Immunology. 2008;146(1):1-10.
doi:10.1159/000112497 .

Ćirković-Veličković, Tanja, Thunberg, Sarah, Polović, Natalija, Neimert-Andersson, Theresa, Gronlund, Hans, van Hage, Marianne, Gafvelin, Guro, "Low levels of endotoxin enhance allergen-stimulated proliferation and reduce the threshold for activation in human peripheral blood cells" in International Archives of Allergy and Immunology, 146, no. 1 (2008):1-10,
https://doi.org/10.1159/000112497 . .