Živković, Suzana

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orcid::0000-0003-0280-5884
  • Živković, Suzana (6)
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Author's Bibliography

Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening

Milutinović, Milica; Nakarada, Đura; Božunović, Jelena; Todorović, Miloš; Gašić, Uroš M.; Živković, Suzana; Skorić, Marijana; Ivković, Đurđa; Savić, Jelena; Devrnja, Nina; Aničić, Neda; Banjanac, Tijana; Mojović, Miloš; Mišić, Danijela

(MDPI, 2023)

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš M.
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5896
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - MDPI
T2  - Antioxidants
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
VL  - 12
IS  - 2
SP  - 346
DO  - 10.3390/antiox12020346
ER  - 
@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš M. and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "MDPI",
journal = "Antioxidants, Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
volume = "12",
number = "2",
pages = "346",
doi = "10.3390/antiox12020346"
}
Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U. M., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346
Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić UM, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .
Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš M., Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .
4
3
1

Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge

Todorović, Slađana; Perić, Marija; Nikolić, Biljana; Mandić, Boris; Cvetković, Stefana; Bogdanović, Milica; Živković, Suzana

(MDPI, 2023)

TY  - JOUR
AU  - Todorović, Slađana
AU  - Perić, Marija
AU  - Nikolić, Biljana
AU  - Mandić, Boris
AU  - Cvetković, Stefana
AU  - Bogdanović, Milica
AU  - Živković, Suzana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6233
AB  - The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.
PB  - MDPI
T2  - Horticulturae
T1  - Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge
VL  - 9
IS  - 3
SP  - 381
DO  - 10.3390/horticulturae9030381
ER  - 
@article{
author = "Todorović, Slađana and Perić, Marija and Nikolić, Biljana and Mandić, Boris and Cvetković, Stefana and Bogdanović, Milica and Živković, Suzana",
year = "2023",
abstract = "The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge",
volume = "9",
number = "3",
pages = "381",
doi = "10.3390/horticulturae9030381"
}
Todorović, S., Perić, M., Nikolić, B., Mandić, B., Cvetković, S., Bogdanović, M.,& Živković, S.. (2023). Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae
MDPI., 9(3), 381.
https://doi.org/10.3390/horticulturae9030381
Todorović S, Perić M, Nikolić B, Mandić B, Cvetković S, Bogdanović M, Živković S. Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae. 2023;9(3):381.
doi:10.3390/horticulturae9030381 .
Todorović, Slađana, Perić, Marija, Nikolić, Biljana, Mandić, Boris, Cvetković, Stefana, Bogdanović, Milica, Živković, Suzana, "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge" in Horticulturae, 9, no. 3 (2023):381,
https://doi.org/10.3390/horticulturae9030381 . .

In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity

Božunović, Jelena; Živković, Suzana; Gašić, Uroš M.; Glamočlija, Jasmina; Ćirić, Ana D.; Matekalo, Dragana; Šiler, Branislav; Soković, Marina; Tešić, Živoslav Lj.; Mišić, Danijela

(Elsevier Science Bv, Amsterdam, 2018)

TY  - JOUR
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Gašić, Uroš M.
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana D.
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Mišić, Danijela
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2579
AB  - The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC-MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in beta-Dglycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/ + HESI - qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity
VL  - 111
SP  - 705
EP  - 721
DO  - 10.1016/j.indcrop.2017.11.040
ER  - 
@article{
author = "Božunović, Jelena and Živković, Suzana and Gašić, Uroš M. and Glamočlija, Jasmina and Ćirić, Ana D. and Matekalo, Dragana and Šiler, Branislav and Soković, Marina and Tešić, Živoslav Lj. and Mišić, Danijela",
year = "2018",
abstract = "The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC-MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in beta-Dglycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/ + HESI - qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity",
volume = "111",
pages = "705-721",
doi = "10.1016/j.indcrop.2017.11.040"
}
Božunović, J., Živković, S., Gašić, U. M., Glamočlija, J., Ćirić, A. D., Matekalo, D., Šiler, B., Soković, M., Tešić, Ž. Lj.,& Mišić, D.. (2018). In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 111, 705-721.
https://doi.org/10.1016/j.indcrop.2017.11.040
Božunović J, Živković S, Gašić UM, Glamočlija J, Ćirić AD, Matekalo D, Šiler B, Soković M, Tešić ŽL, Mišić D. In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity. in Industrial Crops and Products. 2018;111:705-721.
doi:10.1016/j.indcrop.2017.11.040 .
Božunović, Jelena, Živković, Suzana, Gašić, Uroš M., Glamočlija, Jasmina, Ćirić, Ana D., Matekalo, Dragana, Šiler, Branislav, Soković, Marina, Tešić, Živoslav Lj., Mišić, Danijela, "In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity" in Industrial Crops and Products, 111 (2018):705-721,
https://doi.org/10.1016/j.indcrop.2017.11.040 . .
25
15
25

Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040

Božunović, Jelena; Živković, Suzana; Gašić, Uroš M.; Glamočlija, Jasmina; Ćirić, Ana D.; Matekalo, Dragana; Šiler, Branislav; Soković, Marina; Tešić, Živoslav Lj.; Mišić, Danijela

(Elsevier Science Bv, Amsterdam, 2018)

TY  - DATA
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Gašić, Uroš M.
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana D.
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Mišić, Danijela
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3208
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3208
ER  - 
@misc{
author = "Božunović, Jelena and Živković, Suzana and Gašić, Uroš M. and Glamočlija, Jasmina and Ćirić, Ana D. and Matekalo, Dragana and Šiler, Branislav and Soković, Marina and Tešić, Živoslav Lj. and Mišić, Danijela",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3208"
}
Božunović, J., Živković, S., Gašić, U. M., Glamočlija, J., Ćirić, A. D., Matekalo, D., Šiler, B., Soković, M., Tešić, Ž. Lj.,& Mišić, D.. (2018). Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3208
Božunović J, Živković S, Gašić UM, Glamočlija J, Ćirić AD, Matekalo D, Šiler B, Soković M, Tešić ŽL, Mišić D. Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040. in Industrial Crops and Products. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3208 .
Božunović, Jelena, Živković, Suzana, Gašić, Uroš M., Glamočlija, Jasmina, Ćirić, Ana D., Matekalo, Dragana, Šiler, Branislav, Soković, Marina, Tešić, Živoslav Lj., Mišić, Danijela, "Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040" in Industrial Crops and Products (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3208 .

Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies

Mišić, Danijela; Šiler, Branislav; Gašić, Uroš M.; Avramov, Stevan N.; Živković, Suzana; Nestorović Živković, Jasmina; Milutinovic, Milica; Tešić, Živoslav Lj.

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Avramov, Stevan N.
AU  - Živković, Suzana
AU  - Nestorović Živković, Jasmina
AU  - Milutinovic, Milica
AU  - Tešić, Živoslav Lj.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1663
AB  - Introduction - Nepeta species contain a variety of secondary metabolites, including iridoid monoterpenes - nepetalactones and phenolic acids - that are considered the main bioactive constituents. This work represents the first attempt to comparatively explore variations in these two major groups of secondary metabolites within the genus. Objective - To develop an efficient analytical methodology for simultaneous analysis of nepetalactones and phenolic acids in methanol extracts of selected Nepeta species, and to evaluate its potential application in chemotaxonomic studies. Material and methods - A UHPLC combined with linear-trap quadrupole (LTQ) orbitrap MS method was used to characterise chemical diversity and complexity of phenolics among 12 selected Nepeta species. A targeted metabolomic approach using UHPLC coupled to a diode array detector (DAD) and combined with (+/-) heated electrospray ionisation (HESI) MS/MS was developed and validated for quantitative analysis of six hydroxycinnamic acid derivatives and four nepetalactones. Results - Phenolic profiling provided a valuable database of bioactive compounds in the plant group studied, including phenolic acids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavones, flavonols and flavanones). Principal component analysis and cluster analysis suggested the applicability of 10 targeted compounds as chemomarkers for chemotaxonomic studies. Pearson's correlation analysis revealed significant positive correlations between metabolites involved in different biosynthetic pathways (phenylpropanoid or monoterpenoid). Conclusion - The described targeted metabolomic approach proved to be highly beneficial in designing a phytochemical overview of the genus Nepeta, and might have applications in further clarification of phylogenetic relations. Furthermore, it has the potential to be implemented in a routine quality control of plant material and herbal preparations.
PB  - Wiley-Blackwell, Hoboken
T2  - Phytochemical Analysis
T1  - Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies
VL  - 26
IS  - 1
SP  - 72
EP  - 85
DO  - 10.1002/pca.2538
ER  - 
@article{
author = "Mišić, Danijela and Šiler, Branislav and Gašić, Uroš M. and Avramov, Stevan N. and Živković, Suzana and Nestorović Živković, Jasmina and Milutinovic, Milica and Tešić, Živoslav Lj.",
year = "2015",
abstract = "Introduction - Nepeta species contain a variety of secondary metabolites, including iridoid monoterpenes - nepetalactones and phenolic acids - that are considered the main bioactive constituents. This work represents the first attempt to comparatively explore variations in these two major groups of secondary metabolites within the genus. Objective - To develop an efficient analytical methodology for simultaneous analysis of nepetalactones and phenolic acids in methanol extracts of selected Nepeta species, and to evaluate its potential application in chemotaxonomic studies. Material and methods - A UHPLC combined with linear-trap quadrupole (LTQ) orbitrap MS method was used to characterise chemical diversity and complexity of phenolics among 12 selected Nepeta species. A targeted metabolomic approach using UHPLC coupled to a diode array detector (DAD) and combined with (+/-) heated electrospray ionisation (HESI) MS/MS was developed and validated for quantitative analysis of six hydroxycinnamic acid derivatives and four nepetalactones. Results - Phenolic profiling provided a valuable database of bioactive compounds in the plant group studied, including phenolic acids (hydroxybenzoic and hydroxycinnamic acids) and flavonoids (flavones, flavonols and flavanones). Principal component analysis and cluster analysis suggested the applicability of 10 targeted compounds as chemomarkers for chemotaxonomic studies. Pearson's correlation analysis revealed significant positive correlations between metabolites involved in different biosynthetic pathways (phenylpropanoid or monoterpenoid). Conclusion - The described targeted metabolomic approach proved to be highly beneficial in designing a phytochemical overview of the genus Nepeta, and might have applications in further clarification of phylogenetic relations. Furthermore, it has the potential to be implemented in a routine quality control of plant material and herbal preparations.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytochemical Analysis",
title = "Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies",
volume = "26",
number = "1",
pages = "72-85",
doi = "10.1002/pca.2538"
}
Mišić, D., Šiler, B., Gašić, U. M., Avramov, S. N., Živković, S., Nestorović Živković, J., Milutinovic, M.,& Tešić, Ž. Lj.. (2015). Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. in Phytochemical Analysis
Wiley-Blackwell, Hoboken., 26(1), 72-85.
https://doi.org/10.1002/pca.2538
Mišić D, Šiler B, Gašić UM, Avramov SN, Živković S, Nestorović Živković J, Milutinovic M, Tešić ŽL. Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. in Phytochemical Analysis. 2015;26(1):72-85.
doi:10.1002/pca.2538 .
Mišić, Danijela, Šiler, Branislav, Gašić, Uroš M., Avramov, Stevan N., Živković, Suzana, Nestorović Živković, Jasmina, Milutinovic, Milica, Tešić, Živoslav Lj., "Simultaneous UHPLC/DAD/( plus /-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies" in Phytochemical Analysis, 26, no. 1 (2015):72-85,
https://doi.org/10.1002/pca.2538 . .
74
51
76

Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538

Mišić, Danijela; Šiler, Branislav; Gašić, Uroš M.; Avramov, Stevan N.; Živković, Suzana; Nestorović Živković, Jasmina; Milutinovic, Milica; Tešić, Živoslav Lj.

(Wiley-Blackwell, Hoboken, 2015)

TY  - DATA
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Avramov, Stevan N.
AU  - Živković, Suzana
AU  - Nestorović Živković, Jasmina
AU  - Milutinovic, Milica
AU  - Tešić, Živoslav Lj.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3420
PB  - Wiley-Blackwell, Hoboken
T2  - Phytochemical Analysis
T1  - Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3420
ER  - 
@misc{
author = "Mišić, Danijela and Šiler, Branislav and Gašić, Uroš M. and Avramov, Stevan N. and Živković, Suzana and Nestorović Živković, Jasmina and Milutinovic, Milica and Tešić, Živoslav Lj.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytochemical Analysis",
title = "Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3420"
}
Mišić, D., Šiler, B., Gašić, U. M., Avramov, S. N., Živković, S., Nestorović Živković, J., Milutinovic, M.,& Tešić, Ž. Lj.. (2015). Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538. in Phytochemical Analysis
Wiley-Blackwell, Hoboken..
https://hdl.handle.net/21.15107/rcub_cherry_3420
Mišić D, Šiler B, Gašić UM, Avramov SN, Živković S, Nestorović Živković J, Milutinovic M, Tešić ŽL. Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538. in Phytochemical Analysis. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3420 .
Mišić, Danijela, Šiler, Branislav, Gašić, Uroš M., Avramov, Stevan N., Živković, Suzana, Nestorović Živković, Jasmina, Milutinovic, Milica, Tešić, Živoslav Lj., "Supplementary data for article: Mišić, D.; Šiler, B.; Gašić, U.; Avramov, S.; Živković, S.; Živković, J. N.; Milutinović, M.; Tešić, Ž. Simultaneous UHPLC/DAD/(+/-)HESI-MS/MS Analysis of Phenolic Acids and Nepetalactones in Methanol Extracts of Nepeta Species: A Possible Application in Chemotaxonomic Studies. Phytochemical Analysis 2015, 26 (1), 72–85. https://doi.org/10.1002/pca.2538" in Phytochemical Analysis (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3420 .