TY  - JOUR
AU  - Marić, Nenad
AU  - Štrbački, Jana
AU  - Polk, Jason
AU  - Slavković-Beškoski, Latinka
AU  - Avdalović, Jelena
AU  - Lješević, Marija
AU  - Joksimović, Kristina
AU  - Žerađanin, Aleksandra
AU  - Beškoski, Vladimir
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5250
AB  - The characterization and cleanup of groundwater contamination in hazardous-waste sites are of significant engineering and scientific importance. This study analyzes biodegradation fingerprints in groundwater, 25 years after the accidental leakage of kerosene (jet fuel) at the site in central Serbia. The long-term existence of hydrocarbon contamination and the presence of indigenous microbiological populations capable of biodegradation have been confirmed by the results of GCxGC-MS chromatography and microbiological characterization. Total petroleum hydrocarbons (TPH), geochemical indicators, and other parameters were spatially analyzed to provide insight into biodegradation occurrence. The lowest concentrations of electron acceptors (O2, NO3-, and SO42-) and the highest concentrations of microbial metabolic products (Mn and Fe) overlap in the piezometers closest to the source of contamination, due to the occurrence of different biodegradation mechanisms. Based on the analysis of redox-sensitive compounds, the mixed oxic-anoxic processes in groundwater also correspond to the aforementioned zone, as confirmed by the redox potential (Eh) measurements. The results of cross-study analysis reveal further reduction of hydrocarbon contamination, the occurrence of less anoxic redox processes, and the increase of oxic conditions in groundwater. Overall, the results provide clear evidence of the biodegradation effects in groundwater and insight into their evolution under natural attenuation processes.
PB  - England: Taylor and Francis
T2  - Chemistry and Ecology
T1  - Spatial–temporal assessment of hydrocarbon biodegradation mechanisms at a contaminated groundwater site in Serbia
VL  - 38
IS  - 2
SP  - 95
EP  - 107
DO  - 10.1080/02757540.2021.2017903
ER  - 

@article{
author = "Marić, Nenad and Štrbački, Jana and Polk, Jason and Slavković-Beškoski, Latinka and Avdalović, Jelena and Lješević, Marija and Joksimović, Kristina and Žerađanin, Aleksandra and Beškoski, Vladimir",
year = "2022",
abstract = "The characterization and cleanup of groundwater contamination in hazardous-waste sites are of significant engineering and scientific importance. This study analyzes biodegradation fingerprints in groundwater, 25 years after the accidental leakage of kerosene (jet fuel) at the site in central Serbia. The long-term existence of hydrocarbon contamination and the presence of indigenous microbiological populations capable of biodegradation have been confirmed by the results of GCxGC-MS chromatography and microbiological characterization. Total petroleum hydrocarbons (TPH), geochemical indicators, and other parameters were spatially analyzed to provide insight into biodegradation occurrence. The lowest concentrations of electron acceptors (O2, NO3-, and SO42-) and the highest concentrations of microbial metabolic products (Mn and Fe) overlap in the piezometers closest to the source of contamination, due to the occurrence of different biodegradation mechanisms. Based on the analysis of redox-sensitive compounds, the mixed oxic-anoxic processes in groundwater also correspond to the aforementioned zone, as confirmed by the redox potential (Eh) measurements. The results of cross-study analysis reveal further reduction of hydrocarbon contamination, the occurrence of less anoxic redox processes, and the increase of oxic conditions in groundwater. Overall, the results provide clear evidence of the biodegradation effects in groundwater and insight into their evolution under natural attenuation processes.",
publisher = "England: Taylor and Francis",
journal = "Chemistry and Ecology",
title = "Spatial–temporal assessment of hydrocarbon biodegradation mechanisms at a contaminated groundwater site in Serbia",
volume = "38",
number = "2",
pages = "95-107",
doi = "10.1080/02757540.2021.2017903"
}

Marić, N., Štrbački, J., Polk, J., Slavković-Beškoski, L., Avdalović, J., Lješević, M., Joksimović, K., Žerađanin, A.,& Beškoski, V.. (2022). Spatial–temporal assessment of hydrocarbon biodegradation mechanisms at a contaminated groundwater site in Serbia. in Chemistry and Ecology
England: Taylor and Francis., 38(2), 95-107.
https://doi.org/10.1080/02757540.2021.2017903

Marić N, Štrbački J, Polk J, Slavković-Beškoski L, Avdalović J, Lješević M, Joksimović K, Žerađanin A, Beškoski V. Spatial–temporal assessment of hydrocarbon biodegradation mechanisms at a contaminated groundwater site in Serbia. in Chemistry and Ecology. 2022;38(2):95-107.
doi:10.1080/02757540.2021.2017903 .

Marić, Nenad, Štrbački, Jana, Polk, Jason, Slavković-Beškoski, Latinka, Avdalović, Jelena, Lješević, Marija, Joksimović, Kristina, Žerađanin, Aleksandra, Beškoski, Vladimir, "Spatial–temporal assessment of hydrocarbon biodegradation mechanisms at a contaminated groundwater site in Serbia" in Chemistry and Ecology, 38, no. 2 (2022):95-107,
https://doi.org/10.1080/02757540.2021.2017903 . .

TY  - JOUR
AU  - Bulatović, Sandra
AU  - Marić, Nenad
AU  - Šolević-Knudsen, Tatjana
AU  - Avdalović, Jelena
AU  - Ilić, Mila V.
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4142
AB  - Due to their extensive use, petroleum hydrocarbons are among the most common groundwater contaminants. Compared to the traditional methods of physical pumping of contamination from the aquifer and subsequent treatment (i.e., pump and treat), bioremediation is an economically cost-effective technology. The aim of this remediation approach is to transform biologically contaminants, most often by microbiological activity, into non-toxic compounds. More precisely, it is an active remediation process that involves biostimulation (increase of aquifer oxygenation, addition of nutrients) and/or bioaugmentation (injection of a concentrated and specialized population of microorganisms). Using both biostimulation and bioaugmentation, enhanced in situ groundwater bioremediation was applied at a hydrocarbon-contaminated site in Belgrade. The bioremediation treatment, applied over twelve months, was highly efficient in reducing the concentrations of total petroleum hydrocarbon (TPH) to acceptable levels. The concentration of TPH in the piezometer P-5 was reduced by 98.55 %, in the piezometer P-6 by 98.30 % and in the piezometer P-7 by 98.09 %. These results provided strong evidence on the potential of this remediation approach to overcome site-limiting factors and enhance microbiological activity in order to reduce groundwater contamination. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 43004]
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)
VL  - 85
IS  - 8
SP  - 1067
EP  - 1081
DO  - 10.2298/JSC191023003B
ER  - 

@article{
author = "Bulatović, Sandra and Marić, Nenad and Šolević-Knudsen, Tatjana and Avdalović, Jelena and Ilić, Mila V. and Jovančićević, Branimir and Vrvić, Miroslav M.",
year = "2020",
abstract = "Due to their extensive use, petroleum hydrocarbons are among the most common groundwater contaminants. Compared to the traditional methods of physical pumping of contamination from the aquifer and subsequent treatment (i.e., pump and treat), bioremediation is an economically cost-effective technology. The aim of this remediation approach is to transform biologically contaminants, most often by microbiological activity, into non-toxic compounds. More precisely, it is an active remediation process that involves biostimulation (increase of aquifer oxygenation, addition of nutrients) and/or bioaugmentation (injection of a concentrated and specialized population of microorganisms). Using both biostimulation and bioaugmentation, enhanced in situ groundwater bioremediation was applied at a hydrocarbon-contaminated site in Belgrade. The bioremediation treatment, applied over twelve months, was highly efficient in reducing the concentrations of total petroleum hydrocarbon (TPH) to acceptable levels. The concentration of TPH in the piezometer P-5 was reduced by 98.55 %, in the piezometer P-6 by 98.30 % and in the piezometer P-7 by 98.09 %. These results provided strong evidence on the potential of this remediation approach to overcome site-limiting factors and enhance microbiological activity in order to reduce groundwater contamination. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 43004]",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)",
volume = "85",
number = "8",
pages = "1067-1081",
doi = "10.2298/JSC191023003B"
}

Bulatović, S., Marić, N., Šolević-Knudsen, T., Avdalović, J., Ilić, M. V., Jovančićević, B.,& Vrvić, M. M.. (2020). Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia). in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 85(8), 1067-1081.
https://doi.org/10.2298/JSC191023003B

Bulatović S, Marić N, Šolević-Knudsen T, Avdalović J, Ilić MV, Jovančićević B, Vrvić MM. Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia). in Journal of the Serbian Chemical Society. 2020;85(8):1067-1081.
doi:10.2298/JSC191023003B .

Bulatović, Sandra, Marić, Nenad, Šolević-Knudsen, Tatjana, Avdalović, Jelena, Ilić, Mila V., Jovančićević, Branimir, Vrvić, Miroslav M., "Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)" in Journal of the Serbian Chemical Society, 85, no. 8 (2020):1067-1081,
https://doi.org/10.2298/JSC191023003B . .

TY  - JOUR
AU  - Marić, Nenad
AU  - Štrbački, Jana
AU  - Mrazovac Kurilić, Sanja
AU  - Beškoski, Vladimir
AU  - Nikić, Zoran
AU  - Ignjatović, Snežana
AU  - Malbašić, Jovana
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4012
AB  - Aquifer contamination by petroleum hydrocarbons leads to measurable changes of groundwater hydrochemistry, primarily due to the microbiological activity. This study analyzes this phenomenon at an historical kerosene-contaminated site in Vitanovac (central Serbia). Due to the long-term hydrocarbon contamination and exposure to aerobic and anaerobic biodegradation mechanisms, the lowest concentrations of O2, NO3−, and SO42− (electron acceptors) and the highest concentrations of Mn and Fe (products of microbial metabolic activity) overlap. The terminal redox-accepting processes in groundwater ranged from oxygen reduction to sulfate reduction. The most anoxic processes were registered in piezometers closest to the source of contamination, as was also confirmed by the redox potential (Eh) measurements. High electrical conductivity values and the highest TOC, SiO2, and Al concentrations also overlap in the contaminated zone close to the source of contamination. Scanning electron microscopy study of quartz grains from the zone undergoing the impact of biodegradation confirmed the occurrence of weathering microscale processes on mineral surfaces. Taking all these factors together, it seems reasonable to assume that microbiological activity has caused the enhanced weathering of silicate minerals.
PB  - Springer
T2  - Environmental Geochemistry and Health
T1  - Hydrochemistry of groundwater contaminated by petroleum hydrocarbons: the impact of biodegradation (Vitanovac, Serbia)
VL  - 42
IS  - 7
SP  - 1921
EP  - 1935
DO  - 10.1007/s10653-019-00462-9
ER  - 

@article{
author = "Marić, Nenad and Štrbački, Jana and Mrazovac Kurilić, Sanja and Beškoski, Vladimir and Nikić, Zoran and Ignjatović, Snežana and Malbašić, Jovana",
year = "2020",
abstract = "Aquifer contamination by petroleum hydrocarbons leads to measurable changes of groundwater hydrochemistry, primarily due to the microbiological activity. This study analyzes this phenomenon at an historical kerosene-contaminated site in Vitanovac (central Serbia). Due to the long-term hydrocarbon contamination and exposure to aerobic and anaerobic biodegradation mechanisms, the lowest concentrations of O2, NO3−, and SO42− (electron acceptors) and the highest concentrations of Mn and Fe (products of microbial metabolic activity) overlap. The terminal redox-accepting processes in groundwater ranged from oxygen reduction to sulfate reduction. The most anoxic processes were registered in piezometers closest to the source of contamination, as was also confirmed by the redox potential (Eh) measurements. High electrical conductivity values and the highest TOC, SiO2, and Al concentrations also overlap in the contaminated zone close to the source of contamination. Scanning electron microscopy study of quartz grains from the zone undergoing the impact of biodegradation confirmed the occurrence of weathering microscale processes on mineral surfaces. Taking all these factors together, it seems reasonable to assume that microbiological activity has caused the enhanced weathering of silicate minerals.",
publisher = "Springer",
journal = "Environmental Geochemistry and Health",
title = "Hydrochemistry of groundwater contaminated by petroleum hydrocarbons: the impact of biodegradation (Vitanovac, Serbia)",
volume = "42",
number = "7",
pages = "1921-1935",
doi = "10.1007/s10653-019-00462-9"
}

Marić, N., Štrbački, J., Mrazovac Kurilić, S., Beškoski, V., Nikić, Z., Ignjatović, S.,& Malbašić, J.. (2020). Hydrochemistry of groundwater contaminated by petroleum hydrocarbons: the impact of biodegradation (Vitanovac, Serbia). in Environmental Geochemistry and Health
Springer., 42(7), 1921-1935.
https://doi.org/10.1007/s10653-019-00462-9

Marić N, Štrbački J, Mrazovac Kurilić S, Beškoski V, Nikić Z, Ignjatović S, Malbašić J. Hydrochemistry of groundwater contaminated by petroleum hydrocarbons: the impact of biodegradation (Vitanovac, Serbia). in Environmental Geochemistry and Health. 2020;42(7):1921-1935.
doi:10.1007/s10653-019-00462-9 .

Marić, Nenad, Štrbački, Jana, Mrazovac Kurilić, Sanja, Beškoski, Vladimir, Nikić, Zoran, Ignjatović, Snežana, Malbašić, Jovana, "Hydrochemistry of groundwater contaminated by petroleum hydrocarbons: the impact of biodegradation (Vitanovac, Serbia)" in Environmental Geochemistry and Health, 42, no. 7 (2020):1921-1935,
https://doi.org/10.1007/s10653-019-00462-9 . .

TY  - JOUR
AU  - Marić, Nenad
AU  - Matić, Ivan
AU  - Papic, Petar
AU  - Beškoski, Vladimir
AU  - Ilić, Mila V.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Miletić, Srđan B.
AU  - Nikić, Zoran
AU  - Vrvić, Miroslav M.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2079
AB  - The role of natural attenuation processes in groundwater contamination by petroleum hydrocarbons is of intense scientific and practical interest. This study provides insight into the biodegradation effects in groundwater at a site contaminated by kerosene (jet fuel) in 1993 (Vitanovac, Serbia). Total petroleum hydrocarbons (TPH), hydrochemical indicators (O-2 , NO3-, Mn, Fe, SO42-, HCO3- ), delta C-13 of dissolved inorganic carbon (DIC), and other parameters were measured to demonstrate biodegradation effects in groundwater at the contaminated site. Due to different biodegradation mechanisms, the zone of the lowest concentrations of electron acceptors and the zone of the highest concentrations of metabolic products of biodegradation overlap. Based on the analysis of redox-sensitive compounds in groundwater samples, redox processes ranged from strictly anoxic (methanogenesis) to oxic (oxygen reduction) within a short distance. The dependence of groundwater redox conditions on the distance from the source of contamination was observed. delta C-13 values of DIC ranged from -15.83 to -2.75 parts per thousand, and the most positive values correspond to the zone under anaerobic and methanogenic conditions. Overall, results obtained provide clear evidence on the effects of natural attenuation processes-the activity of biodegradation mechanisms in field conditions.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia)
VL  - 190
IS  - 2
DO  - 10.1007/s10661-018-6462-4
ER  - 

@article{
author = "Marić, Nenad and Matić, Ivan and Papic, Petar and Beškoski, Vladimir and Ilić, Mila V. and Gojgić-Cvijović, Gordana D. and Miletić, Srđan B. and Nikić, Zoran and Vrvić, Miroslav M.",
year = "2018",
abstract = "The role of natural attenuation processes in groundwater contamination by petroleum hydrocarbons is of intense scientific and practical interest. This study provides insight into the biodegradation effects in groundwater at a site contaminated by kerosene (jet fuel) in 1993 (Vitanovac, Serbia). Total petroleum hydrocarbons (TPH), hydrochemical indicators (O-2 , NO3-, Mn, Fe, SO42-, HCO3- ), delta C-13 of dissolved inorganic carbon (DIC), and other parameters were measured to demonstrate biodegradation effects in groundwater at the contaminated site. Due to different biodegradation mechanisms, the zone of the lowest concentrations of electron acceptors and the zone of the highest concentrations of metabolic products of biodegradation overlap. Based on the analysis of redox-sensitive compounds in groundwater samples, redox processes ranged from strictly anoxic (methanogenesis) to oxic (oxygen reduction) within a short distance. The dependence of groundwater redox conditions on the distance from the source of contamination was observed. delta C-13 values of DIC ranged from -15.83 to -2.75 parts per thousand, and the most positive values correspond to the zone under anaerobic and methanogenic conditions. Overall, results obtained provide clear evidence on the effects of natural attenuation processes-the activity of biodegradation mechanisms in field conditions.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia)",
volume = "190",
number = "2",
doi = "10.1007/s10661-018-6462-4"
}

Marić, N., Matić, I., Papic, P., Beškoski, V., Ilić, M. V., Gojgić-Cvijović, G. D., Miletić, S. B., Nikić, Z.,& Vrvić, M. M.. (2018). Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia). in Environmental Monitoring and Assessment
Springer, Dordrecht., 190(2).
https://doi.org/10.1007/s10661-018-6462-4

Marić N, Matić I, Papic P, Beškoski V, Ilić MV, Gojgić-Cvijović GD, Miletić SB, Nikić Z, Vrvić MM. Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia). in Environmental Monitoring and Assessment. 2018;190(2).
doi:10.1007/s10661-018-6462-4 .

Marić, Nenad, Matić, Ivan, Papic, Petar, Beškoski, Vladimir, Ilić, Mila V., Gojgić-Cvijović, Gordana D., Miletić, Srđan B., Nikić, Zoran, Vrvić, Miroslav M., "Natural attenuation of petroleum hydrocarbons-a study of biodegradation effects in groundwater (Vitanovac, Serbia)" in Environmental Monitoring and Assessment, 190, no. 2 (2018),
https://doi.org/10.1007/s10661-018-6462-4 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Miletić, Srđan B.
AU  - Ilić, Mila V.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Papić, Petar
AU  - Marić, Nenad
AU  - Šolević-Knudsen, T.
AU  - Jovančićević, Branimir
AU  - Nakano, Takeshi
AU  - Vrvić, Miroslav M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3045
AB  - The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.
PB  - Wiley, Hoboken
T2  - Clean - Soil, Air, Water
T1  - Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater
VL  - 45
IS  - 2
DO  - 10.1002/clen.201600023
ER  - 

@article{
author = "Beškoski, Vladimir and Miletić, Srđan B. and Ilić, Mila V. and Gojgić-Cvijović, Gordana D. and Papić, Petar and Marić, Nenad and Šolević-Knudsen, T. and Jovančićević, Branimir and Nakano, Takeshi and Vrvić, Miroslav M.",
year = "2017",
abstract = "The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.",
publisher = "Wiley, Hoboken",
journal = "Clean - Soil, Air, Water",
title = "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater",
volume = "45",
number = "2",
doi = "10.1002/clen.201600023"
}

Beškoski, V., Miletić, S. B., Ilić, M. V., Gojgić-Cvijović, G. D., Papić, P., Marić, N., Šolević-Knudsen, T., Jovančićević, B., Nakano, T.,& Vrvić, M. M.. (2017). Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in Clean - Soil, Air, Water
Wiley, Hoboken., 45(2).
https://doi.org/10.1002/clen.201600023

Beškoski V, Miletić SB, Ilić MV, Gojgić-Cvijović GD, Papić P, Marić N, Šolević-Knudsen T, Jovančićević B, Nakano T, Vrvić MM. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in Clean - Soil, Air, Water. 2017;45(2).
doi:10.1002/clen.201600023 .

Beškoski, Vladimir, Miletić, Srđan B., Ilić, Mila V., Gojgić-Cvijović, Gordana D., Papić, Petar, Marić, Nenad, Šolević-Knudsen, T., Jovančićević, Branimir, Nakano, Takeshi, Vrvić, Miroslav M., "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater" in Clean - Soil, Air, Water, 45, no. 2 (2017),
https://doi.org/10.1002/clen.201600023 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Miletić, Srđan B.
AU  - Ilić, Mila V.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Papić, Petar
AU  - Marić, Nenad
AU  - Šolević-Knudsen, Tatjana
AU  - Jovančićević, Branimir
AU  - Nakano, Takeshi
AU  - Vrvić, Miroslav M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2436
AB  - The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.
PB  - Wiley, Hoboken
T2  - CLEAN - Soil Air Water
T1  - Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater
VL  - 45
IS  - 2
DO  - 10.1002/clen.201600023
ER  - 

@article{
author = "Beškoski, Vladimir and Miletić, Srđan B. and Ilić, Mila V. and Gojgić-Cvijović, Gordana D. and Papić, Petar and Marić, Nenad and Šolević-Knudsen, Tatjana and Jovančićević, Branimir and Nakano, Takeshi and Vrvić, Miroslav M.",
year = "2017",
abstract = "The objective of this study was to stimulate microbial biodegradation of petroleum pollution in groundwater and to analyze changes in the abundance and distribution of organic compounds detectable in petroleum. Bioremediation was conducted in a closed bipolar system, by bioaugmentation with consortia of hydrocarbon degrading microorganisms (HD) and biostimulation with nutrients. Comprehensive twodimensional gas chromatography-mass spectrometry (GC X GC-MS) was used to visualize all fractions simultaneously. During the study, the content of total petroleum hydrocarbon (TPH) in groundwater decreased by 92.7% of the initial level, and the average rate of biodegradation was 0.1 mg/L per day. Increased numbers of HD were observed and the dominant genera were Pseudomonas, Rhodococcus, Achromobacter, Bacillus, and Micromonospora. In the first 30 days of bioremediation, there was no significant biodegradation of n-alkanes and petroleum biomarkers - isoprenoids such as pristane and phytane, and polycyclic-saturated hydrocarbons such as terpanes and steranes. However, after 60 days of bioremediation, more than 95% of n-alkanes, terpanes and steranes were biodegraded. Phenanthrene and its methyl-,dimethyl-, and trimethyl-isomers were biodegraded and reduced by more than 99% of their initial levels. However, their decomposition had clearly commenced after just 30 days. This is a somewhat surprising result since it follows that the phenanthrenes were more susceptible to biodegradation than the n-alkanes and isoprenoids. Depending on the microbial community used for bioaugmentation, biodegradation of phenanthrene can precede biodegradation of saturated hydrocarbons.",
publisher = "Wiley, Hoboken",
journal = "CLEAN - Soil Air Water",
title = "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater",
volume = "45",
number = "2",
doi = "10.1002/clen.201600023"
}

Beškoski, V., Miletić, S. B., Ilić, M. V., Gojgić-Cvijović, G. D., Papić, P., Marić, N., Šolević-Knudsen, T., Jovančićević, B., Nakano, T.,& Vrvić, M. M.. (2017). Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in CLEAN - Soil Air Water
Wiley, Hoboken., 45(2).
https://doi.org/10.1002/clen.201600023

Beškoski V, Miletić SB, Ilić MV, Gojgić-Cvijović GD, Papić P, Marić N, Šolević-Knudsen T, Jovančićević B, Nakano T, Vrvić MM. Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater. in CLEAN - Soil Air Water. 2017;45(2).
doi:10.1002/clen.201600023 .

Beškoski, Vladimir, Miletić, Srđan B., Ilić, Mila V., Gojgić-Cvijović, Gordana D., Papić, Petar, Marić, Nenad, Šolević-Knudsen, Tatjana, Jovančićević, Branimir, Nakano, Takeshi, Vrvić, Miroslav M., "Biodegradation of Isoprenoids, Steranes, Terpanes, and Phenanthrenes During In Situ Bioremediation of Petroleum-Contaminated Groundwater" in CLEAN - Soil Air Water, 45, no. 2 (2017),
https://doi.org/10.1002/clen.201600023 . .

TY  - JOUR
AU  - Marić, Nenad
AU  - Petrović, Rastko
AU  - Nikić, Zoran
AU  - Beškoski, Vladimir
AU  - Papić, Petar
AU  - Matić, Ivan
AU  - Vrvić, Miroslav M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/169
AB  - Natural attenuation includes a number of processes that cause the reduction of groundwater contamination by petroleum hydrocarbons. Among these processes biodegradation often plays a major role, since microorganisms use petroleum hydrocarbons as the source of energy and carbon. Application of natural attenuation as a remediation approach requires detailed hydrogeological characterization of the contaminated site and monitoring of attenuation processes. This paper presents the results of monitored natural attenuation at the site of historical contamination by kerosene near Kraljevo. In order to demonstrate effects of biodegradation, following parameters in groundwater were measured: total petroleum hydrocarbons (TPH), electron acceptors (O2, NO3, SO4 2-), metabolic products of biodegradation (Mn, Fe), and redox potential (Eh). Obtained results and methodological approach can be considered useful in conceptualizing future studies of this type.
AB  - Prirodno prečišćavanje objedinjuje niz procesa koji dovode do smanjenja zagađenja podzemnih voda naftnim ugljovodonicima. Biodegradacija često ima glavnu ulogu među ovim procesima, pošto mikroorgranizmi koriste naftne ugljovodonike kao izvor energije i ugljenika. Primena prirodnog prečišćavanja kao remedijacionog tretmana podrazumeva detaljnu hidrogeološku karakterizaciju zagađene lokacije i osmatranje procesa kojim dolazi do smanjenja zagađenja. U radu su prikazani rezultati osmatranja prirodnog prečišćavanja na lokaciji istorijskog zagađenja kerozinom kod Kraljeva. U cilju osmatranja efekata biodegradacije, u podzemnim vodama su vršena merenja sledećih parametara: ukupnih naftnih ugljovodonika (TPH), elektron akceptora (O2, NO3 -, SO4 2-), metaboličkih produkata biodegradacije (Mn, Fe) i redoks potencijala (Eh). Dobijeni rezultati i metodološki pristup mogu se smatrati korisnim kod koncipiranja budućih istraživanja ovog tipa.
T2  - Zaštita materijala
T1  - Natural attenuation of groundwater contaminated by petroleum hydrocarbons: Mechanism, research concept, practical application
T1  - Prirodno prečišćavanje podzemnih voda zagađenih naftnim ugljovodonicima - mehanizam, koncepcija istraživanja, primena u praksi
VL  - 58
IS  - 4
SP  - 445
EP  - 454
DO  - 10.5937/ZasMat1704445M
ER  - 

@article{
author = "Marić, Nenad and Petrović, Rastko and Nikić, Zoran and Beškoski, Vladimir and Papić, Petar and Matić, Ivan and Vrvić, Miroslav M.",
year = "2017",
abstract = "Natural attenuation includes a number of processes that cause the reduction of groundwater contamination by petroleum hydrocarbons. Among these processes biodegradation often plays a major role, since microorganisms use petroleum hydrocarbons as the source of energy and carbon. Application of natural attenuation as a remediation approach requires detailed hydrogeological characterization of the contaminated site and monitoring of attenuation processes. This paper presents the results of monitored natural attenuation at the site of historical contamination by kerosene near Kraljevo. In order to demonstrate effects of biodegradation, following parameters in groundwater were measured: total petroleum hydrocarbons (TPH), electron acceptors (O2, NO3, SO4 2-), metabolic products of biodegradation (Mn, Fe), and redox potential (Eh). Obtained results and methodological approach can be considered useful in conceptualizing future studies of this type., Prirodno prečišćavanje objedinjuje niz procesa koji dovode do smanjenja zagađenja podzemnih voda naftnim ugljovodonicima. Biodegradacija često ima glavnu ulogu među ovim procesima, pošto mikroorgranizmi koriste naftne ugljovodonike kao izvor energije i ugljenika. Primena prirodnog prečišćavanja kao remedijacionog tretmana podrazumeva detaljnu hidrogeološku karakterizaciju zagađene lokacije i osmatranje procesa kojim dolazi do smanjenja zagađenja. U radu su prikazani rezultati osmatranja prirodnog prečišćavanja na lokaciji istorijskog zagađenja kerozinom kod Kraljeva. U cilju osmatranja efekata biodegradacije, u podzemnim vodama su vršena merenja sledećih parametara: ukupnih naftnih ugljovodonika (TPH), elektron akceptora (O2, NO3 -, SO4 2-), metaboličkih produkata biodegradacije (Mn, Fe) i redoks potencijala (Eh). Dobijeni rezultati i metodološki pristup mogu se smatrati korisnim kod koncipiranja budućih istraživanja ovog tipa.",
journal = "Zaštita materijala",
title = "Natural attenuation of groundwater contaminated by petroleum hydrocarbons: Mechanism, research concept, practical application, Prirodno prečišćavanje podzemnih voda zagađenih naftnim ugljovodonicima - mehanizam, koncepcija istraživanja, primena u praksi",
volume = "58",
number = "4",
pages = "445-454",
doi = "10.5937/ZasMat1704445M"
}

Marić, N., Petrović, R., Nikić, Z., Beškoski, V., Papić, P., Matić, I.,& Vrvić, M. M.. (2017). Natural attenuation of groundwater contaminated by petroleum hydrocarbons: Mechanism, research concept, practical application. in Zaštita materijala, 58(4), 445-454.
https://doi.org/10.5937/ZasMat1704445M

Marić N, Petrović R, Nikić Z, Beškoski V, Papić P, Matić I, Vrvić MM. Natural attenuation of groundwater contaminated by petroleum hydrocarbons: Mechanism, research concept, practical application. in Zaštita materijala. 2017;58(4):445-454.
doi:10.5937/ZasMat1704445M .

Marić, Nenad, Petrović, Rastko, Nikić, Zoran, Beškoski, Vladimir, Papić, Petar, Matić, Ivan, Vrvić, Miroslav M., "Natural attenuation of groundwater contaminated by petroleum hydrocarbons: Mechanism, research concept, practical application" in Zaštita materijala, 58, no. 4 (2017):445-454,
https://doi.org/10.5937/ZasMat1704445M . .

TY  - JOUR
AU  - Marić, Nenad
AU  - Ilić, Mila V.
AU  - Miletić, Srđan B.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
AU  - Papic, Petar
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1958
AB  - This study provides insight into the enhanced in situ bioremediation applied for remediation of groundwater contaminated by petroleum hydrocarbons. Activities prior to the application of this remediation approach included removal of the source of groundwater contamination-an underground storage tank and contaminated sediments-from the unsaturated zone. The hydraulic feasibility of this remediation approach was proved by hydraulic conductivity characterization of the site. Enhanced in situ bioremediation was performed by a combination of biostimulation and bioaugmentation within the closed bipolar system (one extraction and two injection wells). Biostimulation was conducted by addition of nutrients, and stimulation of oxidation processes by injection of H2O2, into the aquifer. Bioaugmentation/re-inoculation was achieved by injection of a zymogenous consortium of hydrocarbon degrading microorganisms isolated from the contaminated groundwater. The average extraction capacity was 0.5 l/s, with an average injection capacity of 0.25 l/s per well. The efficiency of the remediation approach was measured by changes in the content of total petroleum hydrocarbon (TPH), total chemoorganoheterotrophic (TC) and hydrocarbon degrading (HD) microorganisms. After biostimulation and bioaugmentation, the number of TC and HD microorganisms started to increase. This was followed by a decrease of TPH concentration in the groundwater from an initial 6.8-0.5 mg/l at the end of the bioremediation. The applied remediation approach was highly efficient and very effective in reducing TPH to acceptable levels. Together, these facts provide strong evidence of its potential for remediation of groundwater contaminated by petroleum hydrocarbons.
PB  - Springer, New York
T2  - Environmental Earth Sciences
T1  - Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia
VL  - 74
IS  - 6
SP  - 5211
EP  - 5219
DO  - 10.1007/s12665-015-4531-3
ER  - 

@article{
author = "Marić, Nenad and Ilić, Mila V. and Miletić, Srđan B. and Gojgić-Cvijović, Gordana D. and Beškoski, Vladimir and Vrvić, Miroslav M. and Papic, Petar",
year = "2015",
abstract = "This study provides insight into the enhanced in situ bioremediation applied for remediation of groundwater contaminated by petroleum hydrocarbons. Activities prior to the application of this remediation approach included removal of the source of groundwater contamination-an underground storage tank and contaminated sediments-from the unsaturated zone. The hydraulic feasibility of this remediation approach was proved by hydraulic conductivity characterization of the site. Enhanced in situ bioremediation was performed by a combination of biostimulation and bioaugmentation within the closed bipolar system (one extraction and two injection wells). Biostimulation was conducted by addition of nutrients, and stimulation of oxidation processes by injection of H2O2, into the aquifer. Bioaugmentation/re-inoculation was achieved by injection of a zymogenous consortium of hydrocarbon degrading microorganisms isolated from the contaminated groundwater. The average extraction capacity was 0.5 l/s, with an average injection capacity of 0.25 l/s per well. The efficiency of the remediation approach was measured by changes in the content of total petroleum hydrocarbon (TPH), total chemoorganoheterotrophic (TC) and hydrocarbon degrading (HD) microorganisms. After biostimulation and bioaugmentation, the number of TC and HD microorganisms started to increase. This was followed by a decrease of TPH concentration in the groundwater from an initial 6.8-0.5 mg/l at the end of the bioremediation. The applied remediation approach was highly efficient and very effective in reducing TPH to acceptable levels. Together, these facts provide strong evidence of its potential for remediation of groundwater contaminated by petroleum hydrocarbons.",
publisher = "Springer, New York",
journal = "Environmental Earth Sciences",
title = "Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia",
volume = "74",
number = "6",
pages = "5211-5219",
doi = "10.1007/s12665-015-4531-3"
}

Marić, N., Ilić, M. V., Miletić, S. B., Gojgić-Cvijović, G. D., Beškoski, V., Vrvić, M. M.,& Papic, P.. (2015). Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia. in Environmental Earth Sciences
Springer, New York., 74(6), 5211-5219.
https://doi.org/10.1007/s12665-015-4531-3

Marić N, Ilić MV, Miletić SB, Gojgić-Cvijović GD, Beškoski V, Vrvić MM, Papic P. Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia. in Environmental Earth Sciences. 2015;74(6):5211-5219.
doi:10.1007/s12665-015-4531-3 .

Marić, Nenad, Ilić, Mila V., Miletić, Srđan B., Gojgić-Cvijović, Gordana D., Beškoski, Vladimir, Vrvić, Miroslav M., Papic, Petar, "Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia" in Environmental Earth Sciences, 74, no. 6 (2015):5211-5219,
https://doi.org/10.1007/s12665-015-4531-3 . .

TY  - JOUR
AU  - Marić, Nenad
AU  - Matić, Ivan
AU  - Vrvić, Miroslav M.
AU  - Miletić, Srđan B.
AU  - Ilić, Mila V.
AU  - Beškoski, Vladimir
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/229
AB  - This paper discusses the methods of bioremediation as an approach for remediation of groundwater contaminated by petroleum hydrocarbons. Successful application of these methods largely depends on the site conditions, which results in the necessity of multidisciplinary research at the locations of interest. As part of the research performed at the location of the factory 'Niteks' in Niš, successful 'in situ' bioremediation of groundwater contaminated by petroleum hydrocarbons was carried out. The treatment was performed by a bipolar model with groundwater recirculation, by combination of extraction and injection wells.
AB  - U radu se razmatraju metode bioremedijacije kao pristup u rešavanju problema zagađivanja podzemnih voda naftnim ugljovodonicima. Uspešna primena ovih metoda u velikoj meri zavisi od uslova sredine, što ima za posledicu neophodnost multidisciplinarnog istraživanja lokacija od interesa. Kao deo istraživanja realizovanih na lokaciji fabrike 'Niteks' u Nišu, uspešno je izvedena 'in situ' biormedijacija podzemnih voda zagađenih naftnim ugljovodonicima. Tretman je izveden bipolarnim modelom sa recirkulacijom podzemnih voda pomoću crpnih i upojnih bunara.
T2  - Voda i sanitarna tehnika
T1  - Bioremediation of groundwater at the location 'Niteks' in Niš
T1  - Bioremedijacija podzemnih voda na lokaciji 'Niteks' u Nišu
VL  - 43
IS  - 2
SP  - 17
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_cherry_229
ER  - 

@article{
author = "Marić, Nenad and Matić, Ivan and Vrvić, Miroslav M. and Miletić, Srđan B. and Ilić, Mila V. and Beškoski, Vladimir",
year = "2013",
abstract = "This paper discusses the methods of bioremediation as an approach for remediation of groundwater contaminated by petroleum hydrocarbons. Successful application of these methods largely depends on the site conditions, which results in the necessity of multidisciplinary research at the locations of interest. As part of the research performed at the location of the factory 'Niteks' in Niš, successful 'in situ' bioremediation of groundwater contaminated by petroleum hydrocarbons was carried out. The treatment was performed by a bipolar model with groundwater recirculation, by combination of extraction and injection wells., U radu se razmatraju metode bioremedijacije kao pristup u rešavanju problema zagađivanja podzemnih voda naftnim ugljovodonicima. Uspešna primena ovih metoda u velikoj meri zavisi od uslova sredine, što ima za posledicu neophodnost multidisciplinarnog istraživanja lokacija od interesa. Kao deo istraživanja realizovanih na lokaciji fabrike 'Niteks' u Nišu, uspešno je izvedena 'in situ' biormedijacija podzemnih voda zagađenih naftnim ugljovodonicima. Tretman je izveden bipolarnim modelom sa recirkulacijom podzemnih voda pomoću crpnih i upojnih bunara.",
journal = "Voda i sanitarna tehnika",
title = "Bioremediation of groundwater at the location 'Niteks' in Niš, Bioremedijacija podzemnih voda na lokaciji 'Niteks' u Nišu",
volume = "43",
number = "2",
pages = "17-22",
url = "https://hdl.handle.net/21.15107/rcub_cherry_229"
}

Marić, N., Matić, I., Vrvić, M. M., Miletić, S. B., Ilić, M. V.,& Beškoski, V.. (2013). Bioremediation of groundwater at the location 'Niteks' in Niš. in Voda i sanitarna tehnika, 43(2), 17-22.
https://hdl.handle.net/21.15107/rcub_cherry_229

Marić N, Matić I, Vrvić MM, Miletić SB, Ilić MV, Beškoski V. Bioremediation of groundwater at the location 'Niteks' in Niš. in Voda i sanitarna tehnika. 2013;43(2):17-22.
https://hdl.handle.net/21.15107/rcub_cherry_229 .

Marić, Nenad, Matić, Ivan, Vrvić, Miroslav M., Miletić, Srđan B., Ilić, Mila V., Beškoski, Vladimir, "Bioremediation of groundwater at the location 'Niteks' in Niš" in Voda i sanitarna tehnika, 43, no. 2 (2013):17-22,
https://hdl.handle.net/21.15107/rcub_cherry_229 .