Horvacki, Nikola

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orcid::0000-0002-8347-3318
  • Horvacki, Nikola (6)
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Author's Bibliography

Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products

Jakanovski, Mihajlo V.; Dramićanin, Aleksandra M.; Horvacki, Nikola; Trifković, Jelena; Rašić, Dubravka; Brčić Karačonji, Irena; Milojković-Opsenica, Dušanka

(Beograd : Srpsko hemijsko društvo, 2023)

TY  - CONF
AU  - Jakanovski, Mihajlo V.
AU  - Dramićanin, Aleksandra M.
AU  - Horvacki, Nikola
AU  - Trifković, Jelena
AU  - Rašić, Dubravka
AU  - Brčić Karačonji, Irena
AU  - Milojković-Opsenica, Dušanka
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5933
AB  - Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products
SP  - 73
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5933
ER  - 
@conference{
author = "Jakanovski, Mihajlo V. and Dramićanin, Aleksandra M. and Horvacki, Nikola and Trifković, Jelena and Rašić, Dubravka and Brčić Karačonji, Irena and Milojković-Opsenica, Dušanka",
year = "2023",
abstract = "Considering the well-being cognizance of masses, microgreens and germs have emerged as potential therapeutic functional foods for improving overall health by dietary supplementation. Microgreens and germs have an exceptional volume of various nutrients accounting for higher nutritive benefits compared to their mature counterparts [1]. These plants are difficult to store in fresh, due to their high surface area to volume ratio, high respiration rate, delicate leaves that easily wilt, rapid post-harvest decay transpiration, leakage of nutrient-rich exudates, tissue damage, and early senescence [1]. Therefore, within this study, the effect of drying on the sugar content of 12 samples of different microgreens and germs was monitored. A comparative study of the content of vitamin C and sugar between samples of microgreens and germs in both dry and fresh states was also done. The content of vitamin C was determined by the HPLC method, while the sugar profile was obtained using HPAEC-PAD. The results showed that there was a difference between samples of microgreens and germs (fig. 1), as well as the contents of vitamin C and sugar in the samples are inversely proportional (fig. 2), which is in line with the fact that part of the sugar is converted into vitamin C during ripening, which then serves as an antioxidant and protects against harmful effects [2]. Analyzes also showed that dry samples have a higher sugar content compared to fresh ones (fig.2.), which could serve as an argument to promote cost-effective production and future strategies for maintaining the shelf life and quality of these products in the sense that they do not lose their nutritional value during the drying process.

Fig.1. Principal Component Analysis - The difference between microgreens and germs; score plot (A) – germ samples (G) 1-6, microgreen (MG) samples 7-12, and loading plot (B).

Fig.2. Content of vitamin C (A) and sugars (B) in dry and fresh samples of microgreens (MG) and germs (G).

Acknowledgments: The authors would like to thank the Grina - klico kutak corporation for technical support in the procurement of materials used for experiments. This work has been supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Contract number: 451-03-47/2023-01/200168 and 451-03-47/2023-01/200288.)

References:
[1]	B. P. K. Naik, G. Sekhar, A. Suryakumari, G. S. G. Rajulu, K. Harshini, L.A.S: Deepika, International Journal of Research in Agronomy, 5(1) 2022; 40-42
[2]	S. Kathi, H. Laza, S. Singh, L. Thompson, W. Li, C. Simpson, Scientific reports,12(1) 2022, 13093.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products",
pages = "73-74",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5933"
}
Jakanovski, M. V., Dramićanin, A. M., Horvacki, N., Trifković, J., Rašić, D., Brčić Karačonji, I.,& Milojković-Opsenica, D.. (2023). Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Beograd : Srpsko hemijsko društvo., 73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933
Jakanovski MV, Dramićanin AM, Horvacki N, Trifković J, Rašić D, Brčić Karačonji I, Milojković-Opsenica D. Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products. in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:73-74.
https://hdl.handle.net/21.15107/rcub_cherry_5933 .
Jakanovski, Mihajlo V., Dramićanin, Aleksandra M., Horvacki, Nikola, Trifković, Jelena, Rašić, Dubravka, Brčić Karačonji, Irena, Milojković-Opsenica, Dušanka, "Microgreens and germs: The gleam of next-generation super foods - manipulations in production technologies and future strategies for maintaining the shelf life and quality of products" in XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):73-74,
https://hdl.handle.net/21.15107/rcub_cherry_5933 .

Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia

Horvacki, Nikola; Andrić, Filip; Gašić, Uroš M.; Đurović, Dejan; Tešić, Živoslav Lj.; Fotirić-Akšić, Milica M.; Milojković-Opsenica, Dušanka

(MDPI, 2022)

TY  - JOUR
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš M.
AU  - Đurović, Dejan
AU  - Tešić, Živoslav Lj.
AU  - Fotirić-Akšić, Milica M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5683
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - MDPI
T2  - Molecules
T1  - Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia
VL  - 27
IS  - 21
SP  - 7651
DO  - 10.3390/molecules27217651
ER  - 
@article{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš M. and Đurović, Dejan and Tešić, Živoslav Lj. and Fotirić-Akšić, Milica M. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "MDPI",
journal = "Molecules",
title = "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia",
volume = "27",
number = "21",
pages = "7651",
doi = "10.3390/molecules27217651"
}
Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž. Lj., Fotirić-Akšić, M. M.,& Milojković-Opsenica, D.. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules
MDPI., 27(21), 7651.
https://doi.org/10.3390/molecules27217651
Horvacki N, Andrić F, Gašić UM, Đurović D, Tešić ŽL, Fotirić-Akšić MM, Milojković-Opsenica D. Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules. 2022;27(21):7651.
doi:10.3390/molecules27217651 .
Horvacki, Nikola, Andrić, Filip, Gašić, Uroš M., Đurović, Dejan, Tešić, Živoslav Lj., Fotirić-Akšić, Milica M., Milojković-Opsenica, Dušanka, "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia" in Molecules, 27, no. 21 (2022):7651,
https://doi.org/10.3390/molecules27217651 . .
2
3
1

Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651

Horvacki, Nikola; Andrić, Filip; Gašić, Uroš M.; Đurović, Dejan; Tešić, Živoslav Lj.; Fotirić-Akšić, Milica M.; Milojković-Opsenica, Dušanka

(MDPI, 2022)

TY  - DATA
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš M.
AU  - Đurović, Dejan
AU  - Tešić, Živoslav Lj.
AU  - Fotirić-Akšić, Milica M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5701
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - MDPI
T2  - Molecules
T1  - Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651
VL  - 27
IS  - 21
SP  - 7651
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5701
ER  - 
@misc{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš M. and Đurović, Dejan and Tešić, Živoslav Lj. and Fotirić-Akšić, Milica M. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "MDPI",
journal = "Molecules",
title = "Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651",
volume = "27",
number = "21",
pages = "7651",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5701"
}
Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž. Lj., Fotirić-Akšić, M. M.,& Milojković-Opsenica, D.. (2022). Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651. in Molecules
MDPI., 27(21), 7651.
https://hdl.handle.net/21.15107/rcub_cherry_5701
Horvacki N, Andrić F, Gašić UM, Đurović D, Tešić ŽL, Fotirić-Akšić MM, Milojković-Opsenica D. Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651. in Molecules. 2022;27(21):7651.
https://hdl.handle.net/21.15107/rcub_cherry_5701 .
Horvacki, Nikola, Andrić, Filip, Gašić, Uroš M., Đurović, Dejan, Tešić, Živoslav Lj., Fotirić-Akšić, Milica M., Milojković-Opsenica, Dušanka, "Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651" in Molecules, 27, no. 21 (2022):7651,
https://hdl.handle.net/21.15107/rcub_cherry_5701 .
3

Distribution of polyphenolic and sugar compounds in different buckwheat plant parts

Nešović, Milica; Gašić, Uroš M.; Tosti, Tomislav; Horvacki, Nikola; Nedić, Nebojša; Sredojević, Milica; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav Lj.

(Royal Society of Chemistry, 2021)

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš M.
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav Lj.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4739
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
VL  - 11
IS  - 42
SP  - 25816
EP  - 25829
DO  - 10.1039/D1RA04250E
ER  - 
@article{
author = "Nešović, Milica and Gašić, Uroš M. and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav Lj.",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
volume = "11",
number = "42",
pages = "25816-25829",
doi = "10.1039/D1RA04250E"
}
Nešović, M., Gašić, U. M., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/D1RA04250E
Nešović M, Gašić UM, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić ŽL. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/D1RA04250E .
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/D1RA04250E . .
1
26
1
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19

Supplementary data for the article: Nešović, M.; Gašić, U.; Tosti, T.; Horvacki, N.; Nedić, N.; Sredojević, M.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Distribution of Polyphenolic and Sugar Compounds in Different Buckwheat Plant Parts. RSC Adv. 2021, 11 (42), 25816–25829. https://doi.org/10.1039/D1RA04250E.

Nešović, Milica; Gašić, Uroš M.; Tosti, Tomislav; Horvacki, Nikola; Nedić, Nebojša; Sredojević, Milica; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav Lj.

(Royal Society of Chemistry, 2021)

TY  - DATA
AU  - Nešović, Milica
AU  - Gašić, Uroš M.
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav Lj.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4740
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Supplementary data for the article: Nešović, M.; Gašić, U.; Tosti, T.; Horvacki, N.; Nedić, N.; Sredojević, M.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Distribution of Polyphenolic and Sugar Compounds in Different Buckwheat Plant Parts. RSC Adv. 2021, 11 (42), 25816–25829. https://doi.org/10.1039/D1RA04250E.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4740
ER  - 
@misc{
author = "Nešović, Milica and Gašić, Uroš M. and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav Lj.",
year = "2021",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Supplementary data for the article: Nešović, M.; Gašić, U.; Tosti, T.; Horvacki, N.; Nedić, N.; Sredojević, M.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Distribution of Polyphenolic and Sugar Compounds in Different Buckwheat Plant Parts. RSC Adv. 2021, 11 (42), 25816–25829. https://doi.org/10.1039/D1RA04250E.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4740"
}
Nešović, M., Gašić, U. M., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2021). Supplementary data for the article: Nešović, M.; Gašić, U.; Tosti, T.; Horvacki, N.; Nedić, N.; Sredojević, M.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Distribution of Polyphenolic and Sugar Compounds in Different Buckwheat Plant Parts. RSC Adv. 2021, 11 (42), 25816–25829. https://doi.org/10.1039/D1RA04250E.. in RSC Advances
Royal Society of Chemistry..
https://hdl.handle.net/21.15107/rcub_cherry_4740
Nešović M, Gašić UM, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić ŽL. Supplementary data for the article: Nešović, M.; Gašić, U.; Tosti, T.; Horvacki, N.; Nedić, N.; Sredojević, M.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Distribution of Polyphenolic and Sugar Compounds in Different Buckwheat Plant Parts. RSC Adv. 2021, 11 (42), 25816–25829. https://doi.org/10.1039/D1RA04250E.. in RSC Advances. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4740 .
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Supplementary data for the article: Nešović, M.; Gašić, U.; Tosti, T.; Horvacki, N.; Nedić, N.; Sredojević, M.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Distribution of Polyphenolic and Sugar Compounds in Different Buckwheat Plant Parts. RSC Adv. 2021, 11 (42), 25816–25829. https://doi.org/10.1039/D1RA04250E." in RSC Advances (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4740 .

Polyphenol profile of buckwheat honey, nectar and pollen

Nešović, Milica; Gašić, Uroš M.; Tosti, Tomislav; Horvacki, Nikola; Šikoparija, Branko; Nedić, Nebojša; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav Lj.

(The Royal Society, 2020)

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš M.
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav Lj.
PY  - 2020
UR  - https://royalsocietypublishing.org/doi/10.1098/rsos.201576
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4451
AB  - A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.
PB  - The Royal Society
T2  - Royal Society Open Science
T1  - Polyphenol profile of buckwheat honey, nectar and pollen
VL  - 7
IS  - 12
SP  - 201576
DO  - 10.1098/rsos.201576
ER  - 
@article{
author = "Nešović, Milica and Gašić, Uroš M. and Tosti, Tomislav and Horvacki, Nikola and Šikoparija, Branko and Nedić, Nebojša and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav Lj.",
year = "2020",
abstract = "A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.",
publisher = "The Royal Society",
journal = "Royal Society Open Science",
title = "Polyphenol profile of buckwheat honey, nectar and pollen",
volume = "7",
number = "12",
pages = "201576",
doi = "10.1098/rsos.201576"
}
Nešović, M., Gašić, U. M., Tosti, T., Horvacki, N., Šikoparija, B., Nedić, N., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2020). Polyphenol profile of buckwheat honey, nectar and pollen. in Royal Society Open Science
The Royal Society., 7(12), 201576.
https://doi.org/10.1098/rsos.201576
Nešović M, Gašić UM, Tosti T, Horvacki N, Šikoparija B, Nedić N, Blagojević S, Ignjatović L, Tešić ŽL. Polyphenol profile of buckwheat honey, nectar and pollen. in Royal Society Open Science. 2020;7(12):201576.
doi:10.1098/rsos.201576 .
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Horvacki, Nikola, Šikoparija, Branko, Nedić, Nebojša, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Polyphenol profile of buckwheat honey, nectar and pollen" in Royal Society Open Science, 7, no. 12 (2020):201576,
https://doi.org/10.1098/rsos.201576 . .
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