Mitić-Ćulafić, Dragana

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  • Mitić-Ćulafić, Dragana (5)

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Industrial solid wastes from Ganoderma lucidum extract production: chemical characterization and investigation of antioxidant, photoprotective and cytotoxic activities

Veljović, Sonja; Petrović, Marija; Jovanović, Marina; Mitić-Ćulafić, Dragana; Semen, Tanja Živković; Kostić, Marija; Natić, Maja

(2023)

TY  - JOUR
AU  - Veljović, Sonja
AU  - Petrović, Marija
AU  - Jovanović, Marina
AU  - Mitić-Ćulafić, Dragana
AU  - Semen, Tanja Živković
AU  - Kostić, Marija
AU  - Natić, Maja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6188
AB  - To the best of the author’s knowledge, so far, the utilization of industrial solid Ganoderma lucidum waste was not investigated as a potential source of remaining valuable bioactive compounds. The aim of our study was to chemically characterize the extract prepared with 96% and 70% ethanol utilizing G. lucidum waste from industrial ethanol (GE) and water (GW) extraction. The phenolic profile was assessed by UHPLC-MS/MS, while the amino acid content of selected extracts was done by GC-MS. The chemical composition of the extracts was obtained by ATP-FTIR analysis, and the peak at 1637 cm− 1 of spectra originating from C = O stretching vibration of amide I, was exclusively detected in samples prepared with 70% ethanol. Among eleven amino acids detected in G. lucidum residues, the most abundant was essential amino acid phenylalanine. Phenolic profile revealed p-hydroxybenzoic acid and chlorogenic acid as dominant in all the samples analyzed. The cytotoxic activity against two human cancer cell lines (colon carcinoma (HCT116) and melanoma (Hs294T)), photoprotective activity, and antioxidant activity of G. lucidum residues ethanol extracts (70% and 96%) were examined. The most prominent cytotoxic effect on HCT116 and Hs294T cells was attributed to extract prepared from the water extract production. The sun protection factor (SPF) values of extracts analyzed were found to be in the range from 1.86 (GE96) to 4.80 (GW70). Therefore, G. lucidum solid waste appeared to be a valuable source of bioactive compounds with antioxidant, photoprotective and cytotoxic activities.
T2  - Journal of Food Measurement and Characterization
T1  - Industrial solid wastes from Ganoderma lucidum extract production: chemical characterization and investigation of antioxidant, photoprotective and cytotoxic activities
VL  - 17
IS  - 4
SP  - 3673
EP  - 3682
DO  - 10.1007/s11694-023-01897-6
ER  - 
@article{
author = "Veljović, Sonja and Petrović, Marija and Jovanović, Marina and Mitić-Ćulafić, Dragana and Semen, Tanja Živković and Kostić, Marija and Natić, Maja",
year = "2023",
abstract = "To the best of the author’s knowledge, so far, the utilization of industrial solid Ganoderma lucidum waste was not investigated as a potential source of remaining valuable bioactive compounds. The aim of our study was to chemically characterize the extract prepared with 96% and 70% ethanol utilizing G. lucidum waste from industrial ethanol (GE) and water (GW) extraction. The phenolic profile was assessed by UHPLC-MS/MS, while the amino acid content of selected extracts was done by GC-MS. The chemical composition of the extracts was obtained by ATP-FTIR analysis, and the peak at 1637 cm− 1 of spectra originating from C = O stretching vibration of amide I, was exclusively detected in samples prepared with 70% ethanol. Among eleven amino acids detected in G. lucidum residues, the most abundant was essential amino acid phenylalanine. Phenolic profile revealed p-hydroxybenzoic acid and chlorogenic acid as dominant in all the samples analyzed. The cytotoxic activity against two human cancer cell lines (colon carcinoma (HCT116) and melanoma (Hs294T)), photoprotective activity, and antioxidant activity of G. lucidum residues ethanol extracts (70% and 96%) were examined. The most prominent cytotoxic effect on HCT116 and Hs294T cells was attributed to extract prepared from the water extract production. The sun protection factor (SPF) values of extracts analyzed were found to be in the range from 1.86 (GE96) to 4.80 (GW70). Therefore, G. lucidum solid waste appeared to be a valuable source of bioactive compounds with antioxidant, photoprotective and cytotoxic activities.",
journal = "Journal of Food Measurement and Characterization",
title = "Industrial solid wastes from Ganoderma lucidum extract production: chemical characterization and investigation of antioxidant, photoprotective and cytotoxic activities",
volume = "17",
number = "4",
pages = "3673-3682",
doi = "10.1007/s11694-023-01897-6"
}
Veljović, S., Petrović, M., Jovanović, M., Mitić-Ćulafić, D., Semen, T. Ž., Kostić, M.,& Natić, M.. (2023). Industrial solid wastes from Ganoderma lucidum extract production: chemical characterization and investigation of antioxidant, photoprotective and cytotoxic activities. in Journal of Food Measurement and Characterization, 17(4), 3673-3682.
https://doi.org/10.1007/s11694-023-01897-6
Veljović S, Petrović M, Jovanović M, Mitić-Ćulafić D, Semen TŽ, Kostić M, Natić M. Industrial solid wastes from Ganoderma lucidum extract production: chemical characterization and investigation of antioxidant, photoprotective and cytotoxic activities. in Journal of Food Measurement and Characterization. 2023;17(4):3673-3682.
doi:10.1007/s11694-023-01897-6 .
Veljović, Sonja, Petrović, Marija, Jovanović, Marina, Mitić-Ćulafić, Dragana, Semen, Tanja Živković, Kostić, Marija, Natić, Maja, "Industrial solid wastes from Ganoderma lucidum extract production: chemical characterization and investigation of antioxidant, photoprotective and cytotoxic activities" in Journal of Food Measurement and Characterization, 17, no. 4 (2023):3673-3682,
https://doi.org/10.1007/s11694-023-01897-6 . .
1
1

Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats

Đurašević, Siniša; Nikolić, Gorana; Todorović, Ana; Drakulić, Dunja; Pejić, Snežana; Martinović, Vesna; Mitić-Ćulafić, Dragana; Milić, Dragana; Kop, Tatjana; Jasnić, Nebojša; Popović-Đorđević, Jelena; Todorović, Zoran B.

(Elsevier, 2020)

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Nikolić, Gorana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Martinović, Vesna
AU  - Mitić-Ćulafić, Dragana
AU  - Milić, Dragana
AU  - Kop, Tatjana
AU  - Jasnić, Nebojša
AU  - Popović-Đorđević, Jelena
AU  - Todorović, Zoran B.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3994
AB  - The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats
VL  - 140
DO  - 10.1016/j.fct.2020.111302
ER  - 
@article{
author = "Đurašević, Siniša and Nikolić, Gorana and Todorović, Ana and Drakulić, Dunja and Pejić, Snežana and Martinović, Vesna and Mitić-Ćulafić, Dragana and Milić, Dragana and Kop, Tatjana and Jasnić, Nebojša and Popović-Đorđević, Jelena and Todorović, Zoran B.",
year = "2020",
abstract = "The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats",
volume = "140",
doi = "10.1016/j.fct.2020.111302"
}
Đurašević, S., Nikolić, G., Todorović, A., Drakulić, D., Pejić, S., Martinović, V., Mitić-Ćulafić, D., Milić, D., Kop, T., Jasnić, N., Popović-Đorđević, J.,& Todorović, Z. B.. (2020). Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology
Elsevier., 140.
https://doi.org/10.1016/j.fct.2020.111302
Đurašević S, Nikolić G, Todorović A, Drakulić D, Pejić S, Martinović V, Mitić-Ćulafić D, Milić D, Kop T, Jasnić N, Popović-Đorđević J, Todorović ZB. Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology. 2020;140.
doi:10.1016/j.fct.2020.111302 .
Đurašević, Siniša, Nikolić, Gorana, Todorović, Ana, Drakulić, Dunja, Pejić, Snežana, Martinović, Vesna, Mitić-Ćulafić, Dragana, Milić, Dragana, Kop, Tatjana, Jasnić, Nebojša, Popović-Đorđević, Jelena, Todorović, Zoran B., "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats" in Food and Chemical Toxicology, 140 (2020),
https://doi.org/10.1016/j.fct.2020.111302 . .
1
12
4
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10

The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats

Đurašević, Siniša; Jama, Adel; Jasnić, Nebojša; Vujovic, Predrag; Jovanović, Miloš; Mitić-Ćulafić, Dragana; Knežević-Vukčević, Jelena; Cakić Milosević, Maja; Ilijević, Konstantin; Popović-Đorđević, Jelena

(Mary Ann Liebert, Inc, New Rochelle, 2017)

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Jama, Adel
AU  - Jasnić, Nebojša
AU  - Vujovic, Predrag
AU  - Jovanović, Miloš
AU  - Mitić-Ćulafić, Dragana
AU  - Knežević-Vukčević, Jelena
AU  - Cakić Milosević, Maja
AU  - Ilijević, Konstantin
AU  - Popović-Đorđević, Jelena
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2401
AB  - One of the useful properties of probiotic bacteria is their capacity to bind different targets, thus eliminating them through feces. It is supposed that one of these targets could be cadmium, a widespread environmental toxicant that causes various disturbances in biological systems. This study examined the protective effects of probiotic supplementation against cadmium-induced toxicity in the rat. The experiment was conducted in the course of 5 weeks. Animals were divided into four groups: (1) controls, (2) probiotics treated, (3) cadmium treated, and (4) probiotics + cadmium treated. The cadmium concentration was measured in the blood, liver, kidney, and feces, as well as the blood alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as biomarkers of the liver function. Histomorphological changes in the liver and kidney were also determined. Our results revealed that probiotics combined with cadmium increase this metal concentration in feces. As a result, blood, liver, and kidney Cd levels, as well as blood ALT and AST activities were lessened compared to the rat group treated with cadmium only. Besides, probiotics consumed simultaneously with cadmium attenuated histomorphological changes in the liver and kidney caused by cadmium. The rise in lactobacilli number in feces of rats treated simultaneously with cadmium and probiotics results in strong correlation with the increase of Cd concentration in their feces and the decrease of Cd concentration in their blood. We speculate that probiotics actively contribute to cadmium excretion through feces, probably, by its binding to their bacterial cell wall.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats
VL  - 20
IS  - 2
SP  - 189
EP  - 196
DO  - 10.1089/jmf.2016.0090
ER  - 
@article{
author = "Đurašević, Siniša and Jama, Adel and Jasnić, Nebojša and Vujovic, Predrag and Jovanović, Miloš and Mitić-Ćulafić, Dragana and Knežević-Vukčević, Jelena and Cakić Milosević, Maja and Ilijević, Konstantin and Popović-Đorđević, Jelena",
year = "2017",
abstract = "One of the useful properties of probiotic bacteria is their capacity to bind different targets, thus eliminating them through feces. It is supposed that one of these targets could be cadmium, a widespread environmental toxicant that causes various disturbances in biological systems. This study examined the protective effects of probiotic supplementation against cadmium-induced toxicity in the rat. The experiment was conducted in the course of 5 weeks. Animals were divided into four groups: (1) controls, (2) probiotics treated, (3) cadmium treated, and (4) probiotics + cadmium treated. The cadmium concentration was measured in the blood, liver, kidney, and feces, as well as the blood alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as biomarkers of the liver function. Histomorphological changes in the liver and kidney were also determined. Our results revealed that probiotics combined with cadmium increase this metal concentration in feces. As a result, blood, liver, and kidney Cd levels, as well as blood ALT and AST activities were lessened compared to the rat group treated with cadmium only. Besides, probiotics consumed simultaneously with cadmium attenuated histomorphological changes in the liver and kidney caused by cadmium. The rise in lactobacilli number in feces of rats treated simultaneously with cadmium and probiotics results in strong correlation with the increase of Cd concentration in their feces and the decrease of Cd concentration in their blood. We speculate that probiotics actively contribute to cadmium excretion through feces, probably, by its binding to their bacterial cell wall.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats",
volume = "20",
number = "2",
pages = "189-196",
doi = "10.1089/jmf.2016.0090"
}
Đurašević, S., Jama, A., Jasnić, N., Vujovic, P., Jovanović, M., Mitić-Ćulafić, D., Knežević-Vukčević, J., Cakić Milosević, M., Ilijević, K.,& Popović-Đorđević, J.. (2017). The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 20(2), 189-196.
https://doi.org/10.1089/jmf.2016.0090
Đurašević S, Jama A, Jasnić N, Vujovic P, Jovanović M, Mitić-Ćulafić D, Knežević-Vukčević J, Cakić Milosević M, Ilijević K, Popović-Đorđević J. The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats. in Journal of Medicinal Food. 2017;20(2):189-196.
doi:10.1089/jmf.2016.0090 .
Đurašević, Siniša, Jama, Adel, Jasnić, Nebojša, Vujovic, Predrag, Jovanović, Miloš, Mitić-Ćulafić, Dragana, Knežević-Vukčević, Jelena, Cakić Milosević, Maja, Ilijević, Konstantin, Popović-Đorđević, Jelena, "The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats" in Journal of Medicinal Food, 20, no. 2 (2017):189-196,
https://doi.org/10.1089/jmf.2016.0090 . .
37
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Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0

Mitić-Ćulafić, Dragana; Nikolić, Biljana; Simin, Nataša; Jasnić, Nebojša; Četojević-Simin, Dragana; Krstić-Ristivojević, Maja; Knežević-Vukčević, Jelena

(Springer, Dordrecht, 2016)

TY  - DATA
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Jasnić, Nebojša
AU  - Četojević-Simin, Dragana
AU  - Krstić-Ristivojević, Maja
AU  - Knežević-Vukčević, Jelena
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3482
PB  - Springer, Dordrecht
T2  - Plant Foods for Human Nutrition
T1  - Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3482
ER  - 
@misc{
author = "Mitić-Ćulafić, Dragana and Nikolić, Biljana and Simin, Nataša and Jasnić, Nebojša and Četojević-Simin, Dragana and Krstić-Ristivojević, Maja and Knežević-Vukčević, Jelena",
year = "2016",
publisher = "Springer, Dordrecht",
journal = "Plant Foods for Human Nutrition",
title = "Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3482"
}
Mitić-Ćulafić, D., Nikolić, B., Simin, N., Jasnić, N., Četojević-Simin, D., Krstić-Ristivojević, M.,& Knežević-Vukčević, J.. (2016). Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0. in Plant Foods for Human Nutrition
Springer, Dordrecht..
https://hdl.handle.net/21.15107/rcub_cherry_3482
Mitić-Ćulafić D, Nikolić B, Simin N, Jasnić N, Četojević-Simin D, Krstić-Ristivojević M, Knežević-Vukčević J. Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0. in Plant Foods for Human Nutrition. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3482 .
Mitić-Ćulafić, Dragana, Nikolić, Biljana, Simin, Nataša, Jasnić, Nebojša, Četojević-Simin, Dragana, Krstić-Ristivojević, Maja, Knežević-Vukčević, Jelena, "Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0" in Plant Foods for Human Nutrition (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3482 .

Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase

Mitić-Ćulafić, Dragana; Nikolić, Biljana; Simin, Nataša; Jasnić, Nebojša; Četojević-Simin, Dragana; Krstić-Ristivojević, Maja; Knežević-Vukčević, Jelena

(Springer, Dordrecht, 2016)

TY  - JOUR
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Jasnić, Nebojša
AU  - Četojević-Simin, Dragana
AU  - Krstić-Ristivojević, Maja
AU  - Knežević-Vukčević, Jelena
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2065
AB  - Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.
PB  - Springer, Dordrecht
T2  - Plant Foods for Human Nutrition
T1  - Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase
VL  - 71
IS  - 1
SP  - 28
EP  - 34
DO  - 10.1007/s11130-015-0519-0
ER  - 
@article{
author = "Mitić-Ćulafić, Dragana and Nikolić, Biljana and Simin, Nataša and Jasnić, Nebojša and Četojević-Simin, Dragana and Krstić-Ristivojević, Maja and Knežević-Vukčević, Jelena",
year = "2016",
abstract = "Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.",
publisher = "Springer, Dordrecht",
journal = "Plant Foods for Human Nutrition",
title = "Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase",
volume = "71",
number = "1",
pages = "28-34",
doi = "10.1007/s11130-015-0519-0"
}
Mitić-Ćulafić, D., Nikolić, B., Simin, N., Jasnić, N., Četojević-Simin, D., Krstić-Ristivojević, M.,& Knežević-Vukčević, J.. (2016). Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. in Plant Foods for Human Nutrition
Springer, Dordrecht., 71(1), 28-34.
https://doi.org/10.1007/s11130-015-0519-0
Mitić-Ćulafić D, Nikolić B, Simin N, Jasnić N, Četojević-Simin D, Krstić-Ristivojević M, Knežević-Vukčević J. Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. in Plant Foods for Human Nutrition. 2016;71(1):28-34.
doi:10.1007/s11130-015-0519-0 .
Mitić-Ćulafić, Dragana, Nikolić, Biljana, Simin, Nataša, Jasnić, Nebojša, Četojević-Simin, Dragana, Krstić-Ristivojević, Maja, Knežević-Vukčević, Jelena, "Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase" in Plant Foods for Human Nutrition, 71, no. 1 (2016):28-34,
https://doi.org/10.1007/s11130-015-0519-0 . .
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