TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Dumanović, Jelena
AU  - Kolarž, Predrag
AU  - Sedlarević Zorić, Ana
AU  - Morina, Filis
AU  - Vidović, Marija
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6386
AB  - Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum × citriodorum, and Ocimum basilicum var. purpurascens) were grown under moderate light (about 300 µmol photons m−2 s−1) in a glasshouse or growth chamber and then either transferred to an open field (average daily dose: 29.2 kJ m−2 d−1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m−2 d−1), to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity (TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity, but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance mechanism to high doses of UV-B irradiation.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties
VL  - 24
IS  - 20
SP  - 15350
DO  - 10.3390/ijms242015350
ER  - 

@article{
author = "Milić Komić, Sonja and Živanović, Bojana and Dumanović, Jelena and Kolarž, Predrag and Sedlarević Zorić, Ana and Morina, Filis and Vidović, Marija and Veljović Jovanović, Sonja",
year = "2023",
abstract = "Three basil plant varieties (Ocimum basilicum var. Genovese, Ocimum × citriodorum, and Ocimum basilicum var. purpurascens) were grown under moderate light (about 300 µmol photons m−2 s−1) in a glasshouse or growth chamber and then either transferred to an open field (average daily dose: 29.2 kJ m−2 d−1) or additionally exposed to UV-B irradiation in a growth chamber (29.16 kJ m−2 d−1), to reveal the variety-specific and light-specific acclimation responses. Total antioxidant capacity (TAC), phenolic profile, ascorbate content, and class III peroxidase (POD) activity were used to determine the antioxidant status of leaves under all four light regimes. Exposure to high solar irradiation at the open field resulted in an increase in TAC, total hydroxycinnamic acids (HCAs, especially caffeic acid), flavonoids, and epidermal UV-absorbing substances in all three varieties, as well as a two-fold increase in the leaf dry/fresh weight ratio. The supplemental UV-B irradiation induced preferential accumulation of HCAs (rosmarinic acid) over flavonoids, increased TAC and POD activity, but decreased the ascorbate content in the leaves, and inhibited the accumulation of epidermal flavonoids in all basil varieties. Furthermore, characteristic leaf curling and UV-B-induced inhibition of plant growth were observed in all basil varieties, while a pro-oxidant effect of UV-B was indicated with H2O2 accumulation in the leaves and spotty leaf browning. The extent of these morphological changes, and oxidative damage depended on the basil cultivar, implies a genotype-specific tolerance mechanism to high doses of UV-B irradiation.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties",
volume = "24",
number = "20",
pages = "15350",
doi = "10.3390/ijms242015350"
}

Milić Komić, S., Živanović, B., Dumanović, J., Kolarž, P., Sedlarević Zorić, A., Morina, F., Vidović, M.,& Veljović Jovanović, S.. (2023). Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences
MDPI., 24(20), 15350.
https://doi.org/10.3390/ijms242015350

Milić Komić S, Živanović B, Dumanović J, Kolarž P, Sedlarević Zorić A, Morina F, Vidović M, Veljović Jovanović S. Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties. in International Journal of Molecular Sciences. 2023;24(20):15350.
doi:10.3390/ijms242015350 .

Milić Komić, Sonja, Živanović, Bojana, Dumanović, Jelena, Kolarž, Predrag, Sedlarević Zorić, Ana, Morina, Filis, Vidović, Marija, Veljović Jovanović, Sonja, "Differential Antioxidant Response to Supplemental UV-B Irradiation and Sunlight in Three Basil Varieties" in International Journal of Molecular Sciences, 24, no. 20 (2023):15350,
https://doi.org/10.3390/ijms242015350 . .

TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokić, Ljiljana
AU  - Veljović Jovanović, Sonja
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4266
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI
T2  - Plants
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato
VL  - 9
IS  - 9
SP  - 1147
DO  - 10.3390/plants9091147
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Tosti, Tomislav and Vidović, Marija and Prokić, Ljiljana and Veljović Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI",
journal = "Plants",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato",
volume = "9",
number = "9",
pages = "1147",
doi = "10.3390/plants9091147"
}

Živanović, B., Milić Komić, S., Tosti, T., Vidović, M., Prokić, L.,& Veljović Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato. in Plants
MDPI., 9(9), 1147.
https://doi.org/10.3390/plants9091147

Živanović B, Milić Komić S, Tosti T, Vidović M, Prokić L, Veljović Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato. in Plants. 2020;9(9):1147.
doi:10.3390/plants9091147 .

Živanović, Bojana, Milić Komić, Sonja, Tosti, Tomislav, Vidović, Marija, Prokić, Ljiljana, Veljović Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato" in Plants, 9, no. 9 (2020):1147,
https://doi.org/10.3390/plants9091147 . .