TY  - JOUR
AU  - Marković, Srđan
AU  - Milošević, Jelica
AU  - Đurić, Milica
AU  - Lolić, Aleksandar
AU  - Polović, Natalija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4479
AB  - Papain is a proteolytic enzyme of great commercial value. It is a cysteine protease highly expressed in Carica papaya fruit latex, but also present in papaya leaves. Purification procedures mostly deal with the latex and include a combination of precipitation and/or chromatographic techniques. Due to its solubility, structure and activity characteristics, the pH and salt content play significant roles in handling papain extracts. Here we report a simple, rapid and easily scalable procedure for papain purification from papaya leaves, which contain different contaminants as compared to papaya latex. Sodium chloride precipitation of contaminants at pH 5 followed by ammonium sulphate precipitation resulted in the removal of other leaf proteins and protein fragments from papain solution and about a 3-fold purification. The procedure also benefits from the suppression of autoproteolysis and preservation of the native structure, as confirmed by FTIR analysis, and the high recovery of activity of over 80%.
T2  - Archives of Biological Sciences
T1  - One-step purification and freeze stability of papain at acidic pH values
VL  - 73
IS  - 1
SP  - 57
EP  - 64
DO  - 10.2298/ABS201217001M
ER  - 

@article{
author = "Marković, Srđan and Milošević, Jelica and Đurić, Milica and Lolić, Aleksandar and Polović, Natalija",
year = "2021",
abstract = "Papain is a proteolytic enzyme of great commercial value. It is a cysteine protease highly expressed in Carica papaya fruit latex, but also present in papaya leaves. Purification procedures mostly deal with the latex and include a combination of precipitation and/or chromatographic techniques. Due to its solubility, structure and activity characteristics, the pH and salt content play significant roles in handling papain extracts. Here we report a simple, rapid and easily scalable procedure for papain purification from papaya leaves, which contain different contaminants as compared to papaya latex. Sodium chloride precipitation of contaminants at pH 5 followed by ammonium sulphate precipitation resulted in the removal of other leaf proteins and protein fragments from papain solution and about a 3-fold purification. The procedure also benefits from the suppression of autoproteolysis and preservation of the native structure, as confirmed by FTIR analysis, and the high recovery of activity of over 80%.",
journal = "Archives of Biological Sciences",
title = "One-step purification and freeze stability of papain at acidic pH values",
volume = "73",
number = "1",
pages = "57-64",
doi = "10.2298/ABS201217001M"
}

Marković, S., Milošević, J., Đurić, M., Lolić, A.,& Polović, N.. (2021). One-step purification and freeze stability of papain at acidic pH values. in Archives of Biological Sciences, 73(1), 57-64.
https://doi.org/10.2298/ABS201217001M

Marković S, Milošević J, Đurić M, Lolić A, Polović N. One-step purification and freeze stability of papain at acidic pH values. in Archives of Biological Sciences. 2021;73(1):57-64.
doi:10.2298/ABS201217001M .

Marković, Srđan, Milošević, Jelica, Đurić, Milica, Lolić, Aleksandar, Polović, Natalija, "One-step purification and freeze stability of papain at acidic pH values" in Archives of Biological Sciences, 73, no. 1 (2021):57-64,
https://doi.org/10.2298/ABS201217001M . .

TY  - JOUR
AU  - Tasić, Miloš
AU  - Marković, Srđan
AU  - Anđić, Zoran
AU  - Gavrilovski, Milorad
AU  - Knežević, Marija
AU  - Vasiljević, Rade
PY  - 2009
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5335
AB  - The results of crystallization analysis on casting alloys of CuZn21Al2 type are presented
in this study. The analysis of the process has been performed by acoustic emission method
whereas the crystallization process has refered to the castings obtained by continuous casting
treatment. Actually, the continuous casting simulation has been carried out using convenient selection of gravitational casting process parameters, so the samples with macrostructure characteristic for continuous castings have been got. The laboratory plant for continuous casting simulation and crystallization analysis by acoustic emission method has been also presented in this study. A corresponding algorithm of the control of continuous casting process is worked out, as well as a programme package itself, which enables following of crystallization process during production of continuous cast semifabricates. As a result of those investigation, two types of acoustic emission signals are defined, as follows: signal of acoustic emission during solidification with absolutely correct crystallization and macrostructure of continuous casting and signal of acoustic emission during solidification of castings, in which any type of failure occurs (crack, inclusion, etc.). 
Finally, the results obtained by the analysis of the solidification process show that it is possible to use acoustic emission as a method of recording acoustic emission signal for monitoring the crystallization process at continuous and gravity casting.
PB  - Serbian Foundrymen Society
T2  - Foundry
T1  - Acoustic emission - non-destructive on-line method for quality control of materials
VL  - 48
IS  - 4
SP  - 4
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5335
ER  - 

@article{
author = "Tasić, Miloš and Marković, Srđan and Anđić, Zoran and Gavrilovski, Milorad and Knežević, Marija and Vasiljević, Rade",
year = "2009",
abstract = "The results of crystallization analysis on casting alloys of CuZn21Al2 type are presented
in this study. The analysis of the process has been performed by acoustic emission method
whereas the crystallization process has refered to the castings obtained by continuous casting
treatment. Actually, the continuous casting simulation has been carried out using convenient selection of gravitational casting process parameters, so the samples with macrostructure characteristic for continuous castings have been got. The laboratory plant for continuous casting simulation and crystallization analysis by acoustic emission method has been also presented in this study. A corresponding algorithm of the control of continuous casting process is worked out, as well as a programme package itself, which enables following of crystallization process during production of continuous cast semifabricates. As a result of those investigation, two types of acoustic emission signals are defined, as follows: signal of acoustic emission during solidification with absolutely correct crystallization and macrostructure of continuous casting and signal of acoustic emission during solidification of castings, in which any type of failure occurs (crack, inclusion, etc.). 
Finally, the results obtained by the analysis of the solidification process show that it is possible to use acoustic emission as a method of recording acoustic emission signal for monitoring the crystallization process at continuous and gravity casting.",
publisher = "Serbian Foundrymen Society",
journal = "Foundry",
title = "Acoustic emission - non-destructive on-line method for quality control of materials",
volume = "48",
number = "4",
pages = "4-13",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5335"
}

Tasić, M., Marković, S., Anđić, Z., Gavrilovski, M., Knežević, M.,& Vasiljević, R.. (2009). Acoustic emission - non-destructive on-line method for quality control of materials. in Foundry
Serbian Foundrymen Society., 48(4), 4-13.
https://hdl.handle.net/21.15107/rcub_cherry_5335

Tasić M, Marković S, Anđić Z, Gavrilovski M, Knežević M, Vasiljević R. Acoustic emission - non-destructive on-line method for quality control of materials. in Foundry. 2009;48(4):4-13.
https://hdl.handle.net/21.15107/rcub_cherry_5335 .

Tasić, Miloš, Marković, Srđan, Anđić, Zoran, Gavrilovski, Milorad, Knežević, Marija, Vasiljević, Rade, "Acoustic emission - non-destructive on-line method for quality control of materials" in Foundry, 48, no. 4 (2009):4-13,
https://hdl.handle.net/21.15107/rcub_cherry_5335 .