TY  - CONF
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Lončarević, B.
AU  - Lješević, Marija
AU  - Avdalović, J.
AU  - Ranđelović, Danijela
AU  - Beškoski, Vladimir
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4916
AB  - Introduction: Global worldwide population and urbanization in general have created an increasing need for new energy sources. These sources need to be renewable, but it is also very important to respect the principles of environmental protection. Microbial fuel cells (MFC) are a green technology that is attracting more and more attention in the last decade. MFC presents a system which produces electrical current through metabolic processes of microorganisms such is the decomposition of organic matter. In this process chemical energy is directly converted into electrical energy [1-3]. The performance of MFC depends on several factors: temperature, the composition of the sediment, the material from which the electrodes are made, but certainly, one of the prominent factors is the activity of a microbial community. In this paper, efficiency of two MFC systems will be compared to obtain the highest current and power generation. One of them contains only river sediment as a source of microorganisms, while the other was biostimulated by microorganisms isolated from the same river sediment [2-3]. Methods: The river sediment was placed between a set of inox electrodes in a plastic container, with a total volume of 201 cm3  (MFC I). The second MFC (MFC II) was made in the same way, but a consortium of microorganisms, Clostridium sp., Bacillus sp. and Tepidibacter sp. isolated from the river sediment was added to the sediment. The set of resistors already established in our previous studies were used for the measurement of the amount of voltage, which was then used to obtained the values of current and power [4]. Results: After five days of measuring the generated voltage via MFC I and II, the results for current and power density were obtained. In MFC I, the highest current density was recorded on the fifth day and was 76 mA/ m3  while the power was 1.5 mW/m3 . With MFC II, the results were visibly higher, where the current was increased three times (up to 210 mA/m3 ), and the power by as much as 4 times higher compared to the results of MFC I (6 mW/m3 ). Conclusion: Results show that MFC I has lower values than the sediment stimulated by a consortium of microorganisms in the MFC II. The community of microorganisms greatly contributes to improving the performance of the sediment itself, by generating more power density.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - Influence of Microbial Community on Power Generation Using MFC System
SP  - 112
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4916
ER  - 

@conference{
author = "Joksimović, K. and Žerađanin, A. and Lončarević, B. and Lješević, Marija and Avdalović, J. and Ranđelović, Danijela and Beškoski, Vladimir",
year = "2021",
abstract = "Introduction: Global worldwide population and urbanization in general have created an increasing need for new energy sources. These sources need to be renewable, but it is also very important to respect the principles of environmental protection. Microbial fuel cells (MFC) are a green technology that is attracting more and more attention in the last decade. MFC presents a system which produces electrical current through metabolic processes of microorganisms such is the decomposition of organic matter. In this process chemical energy is directly converted into electrical energy [1-3]. The performance of MFC depends on several factors: temperature, the composition of the sediment, the material from which the electrodes are made, but certainly, one of the prominent factors is the activity of a microbial community. In this paper, efficiency of two MFC systems will be compared to obtain the highest current and power generation. One of them contains only river sediment as a source of microorganisms, while the other was biostimulated by microorganisms isolated from the same river sediment [2-3]. Methods: The river sediment was placed between a set of inox electrodes in a plastic container, with a total volume of 201 cm3  (MFC I). The second MFC (MFC II) was made in the same way, but a consortium of microorganisms, Clostridium sp., Bacillus sp. and Tepidibacter sp. isolated from the river sediment was added to the sediment. The set of resistors already established in our previous studies were used for the measurement of the amount of voltage, which was then used to obtained the values of current and power [4]. Results: After five days of measuring the generated voltage via MFC I and II, the results for current and power density were obtained. In MFC I, the highest current density was recorded on the fifth day and was 76 mA/ m3  while the power was 1.5 mW/m3 . With MFC II, the results were visibly higher, where the current was increased three times (up to 210 mA/m3 ), and the power by as much as 4 times higher compared to the results of MFC I (6 mW/m3 ). Conclusion: Results show that MFC I has lower values than the sediment stimulated by a consortium of microorganisms in the MFC II. The community of microorganisms greatly contributes to improving the performance of the sediment itself, by generating more power density.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "Influence of Microbial Community on Power Generation Using MFC System",
pages = "112-112",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4916"
}

Joksimović, K., Žerađanin, A., Lončarević, B., Lješević, M., Avdalović, J., Ranđelović, D.,& Beškoski, V.. (2021). Influence of Microbial Community on Power Generation Using MFC System. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 112-112.
https://hdl.handle.net/21.15107/rcub_cherry_4916

Joksimović K, Žerađanin A, Lončarević B, Lješević M, Avdalović J, Ranđelović D, Beškoski V. Influence of Microbial Community on Power Generation Using MFC System. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:112-112.
https://hdl.handle.net/21.15107/rcub_cherry_4916 .

Joksimović, K., Žerađanin, A., Lončarević, B., Lješević, Marija, Avdalović, J., Ranđelović, Danijela, Beškoski, Vladimir, "Influence of Microbial Community on Power Generation Using MFC System" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):112-112,
https://hdl.handle.net/21.15107/rcub_cherry_4916 .

TY  - CONF
AU  - Lončarević, B.
AU  - Lješević, Marija
AU  - Marković, M.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Anđelković, I.
AU  - Beškoski, Vladimir
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4929
AB  - Copper is one of the leading metal pollutants in the water, which can cause adverse effects when present in high concentrations. The Daphnia magna is a model organism usually used for the determination of ecotoxicological effects of various compounds since it is highly sensitive to toxic compounds [1]. The aim of this work was to investigate the potential application of microbial extracellular polysaccharides (EPS), levan and pullulan, as agents for reducing the copper toxicity to D. magna. The protective effects of EPS were estimated based on the accumulation of copper in the D. magna cells. Levan is a branched fructane EPS [2] and the one used in this study was produced by Bacillus licheniformis NS032. Pullulan, a linear glucan EPS [3], was produced by Aureobasidium pullulans CH-1. The D. magna were exposed to 50 µg/dm3  of Cu (II) or a combination with 50 mg/dm3  and 100 mg/dm3  of levan or pullulan for 48h in the acute test. Additionally, the prolonged test was performed, where the daphnia were exposed to a 10 µg/dm3  of Cu (II) with or without 50 mg/ dm3  of levan or pullulan for 5 days. After the exposure period, the samples were digested and the accumulation of copper in D. magna was analysed using the iCAP Qc ICP-MS (Thermo Scientific, United Kingdom). The results showed that animals exposed to Cu (II) only, accumulated Cu (II) in a greater amount after the prolonged test compared to the acute one, despite the lower concentration. The treatment with EPS during the acute test increased the copper accumulation for both EPS concentrations tested, whereas during the prolonged exposure test, the Cu (II) accumulation was inhibited. Considering that protective effects of levan and pullulan were observed only with lower copper concentrations and 5 days of exposure, additional experiments are necessary to determine the mechanism of EPS action in order to confirm their possible use as protective agents.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna
SP  - 159
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4929
ER  - 

@conference{
author = "Lončarević, B. and Lješević, Marija and Marković, M. and Gojgić-Cvijović, Gordana D. and Anđelković, I. and Beškoski, Vladimir",
year = "2021",
abstract = "Copper is one of the leading metal pollutants in the water, which can cause adverse effects when present in high concentrations. The Daphnia magna is a model organism usually used for the determination of ecotoxicological effects of various compounds since it is highly sensitive to toxic compounds [1]. The aim of this work was to investigate the potential application of microbial extracellular polysaccharides (EPS), levan and pullulan, as agents for reducing the copper toxicity to D. magna. The protective effects of EPS were estimated based on the accumulation of copper in the D. magna cells. Levan is a branched fructane EPS [2] and the one used in this study was produced by Bacillus licheniformis NS032. Pullulan, a linear glucan EPS [3], was produced by Aureobasidium pullulans CH-1. The D. magna were exposed to 50 µg/dm3  of Cu (II) or a combination with 50 mg/dm3  and 100 mg/dm3  of levan or pullulan for 48h in the acute test. Additionally, the prolonged test was performed, where the daphnia were exposed to a 10 µg/dm3  of Cu (II) with or without 50 mg/ dm3  of levan or pullulan for 5 days. After the exposure period, the samples were digested and the accumulation of copper in D. magna was analysed using the iCAP Qc ICP-MS (Thermo Scientific, United Kingdom). The results showed that animals exposed to Cu (II) only, accumulated Cu (II) in a greater amount after the prolonged test compared to the acute one, despite the lower concentration. The treatment with EPS during the acute test increased the copper accumulation for both EPS concentrations tested, whereas during the prolonged exposure test, the Cu (II) accumulation was inhibited. Considering that protective effects of levan and pullulan were observed only with lower copper concentrations and 5 days of exposure, additional experiments are necessary to determine the mechanism of EPS action in order to confirm their possible use as protective agents.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna",
pages = "159-159",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4929"
}

Lončarević, B., Lješević, M., Marković, M., Gojgić-Cvijović, G. D., Anđelković, I.,& Beškoski, V.. (2021). The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 159-159.
https://hdl.handle.net/21.15107/rcub_cherry_4929

Lončarević B, Lješević M, Marković M, Gojgić-Cvijović GD, Anđelković I, Beškoski V. The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:159-159.
https://hdl.handle.net/21.15107/rcub_cherry_4929 .

Lončarević, B., Lješević, Marija, Marković, M., Gojgić-Cvijović, Gordana D., Anđelković, I., Beškoski, Vladimir, "The Effects of Microbial Polysaccharides on the Copper Accumulation in Daphnia magna" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):159-159,
https://hdl.handle.net/21.15107/rcub_cherry_4929 .