@conference{
author = "Smiljanić, Katarina and Trifunović, Sara and Prodić, Ivana and Divac Rankov, Aleksandra and Ljujic, Mila",
year = "2023",
abstract = "Although e-cigarette are still considered a safer alternative to traditional cigarettes, a
growing body of evidence points to their harmful effects on a range of cellular processes .
In this study lung BEAS 2B epithelial cells were treated for 24 h with sub-cytotoxic
concentration1 of e-cigarette vapour, with and without (w/o) nicotine and (w/o) flavor. The
comprehensive proteome analysis was performed via high resolution mass spectrometry
based proteomics (Orbitrap Exploris 240, Thermo Scientific, USA) and relative, label free
quantification of protein expression and their post-translational and chemical modifications
by PEAKS X Pro Studio (BSI Ltd, Ontario, Canada). E-cigarette liquids induced
significant depletion in total number of proteins and impairment of mitochondrial function
in treated cells. Increased presence of post-translational modifications, including
environmentally-driven toxic&harmful, and those classified as direct oxidative
modifications, were observed especially in combined nicotine+flavour treatment
and flavour treatment without nicotine, beside control, pure nicotine and base
liquid treatments. There is a need to study further biological effects of e-cigarettes in
more details, given their widespread use",
publisher = "Kragujevac : Univerzitet, Prirodno-matematički fakultet",
journal = "Zbornik apstrakata, VI Simpozijum Srpskog udruženja za proteomiku (SePA) “Razvoj i primena novih metoda proteomike”, 2. jun 2023. godine, Kragujevac",
title = "Proteomic and protein modification profiling of lung cells BEAS 2B upon different electronic cigarette vapour treatments",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5950"
}