TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Smiljanić, Katarina
AU  - Takić, Marija
AU  - Sarac, Ivana
AU  - Petovic-Oggiano, Gordana
AU  - Nikolić, Milan
AU  - Jovanović, Vesna B.
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6429
AB  - The binding of ubiquitous serum ligands (free fatty acids) to human serum albumin (HSA)
or its glycation can affect thiol group reactivity, thus influencing its antioxidant activity. The effects
of stearic acid (SA) and glucose binding on HSA structural changes and thiol group content and
reactivity were monitored by fluoroscopy and the Ellman method during a 14-day incubation in
molar ratios to HSA that mimic pathophysiological conditions. Upon incubation with 5 mM glucose,
HSA glycation was the same as HSA without it, in three different HSA:SA molar ratios (HSA:SA-
1:1-2-4). The protective effect of SA on the antioxidant property of HSA under different glucose
regimes (5-10-20 mM) was significantly affected by molar ratios of HSA:SA. Thiol reactivity was fully
restored with 5–20 mM glucose at a 1:1 HSA:SA ratio, while the highest thiol content recovery was in
pathological glucose regimes at a 1:1 HSA:SA ratio. The SA affinity for HSA increased significantly
(1.5- and 1.3-fold, p < 0.01) with 5 and 10 mM glucose compared to the control. These results deepen
the knowledge about the possible regulation of the antioxidant role of HSA in diabetes and other
pathophysiological conditions and enable the design of future HSA-drug studies which, in turn, is
important for clinicians when designing information-based treatments.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - The Thiol Group Reactivity and the Antioxidant Property of Human Serum Albumin Are Controlled by the Joint Action of Fatty Acids and Glucose Binding
VL  - 5
SP  - 2335
DO  - 10.3390/ijms25042335
ER  - 

@article{
author = "Uzelac, Tamara N. and Smiljanić, Katarina and Takić, Marija and Sarac, Ivana and Petovic-Oggiano, Gordana and Nikolić, Milan and Jovanović, Vesna B.",
year = "2024",
abstract = "The binding of ubiquitous serum ligands (free fatty acids) to human serum albumin (HSA)
or its glycation can affect thiol group reactivity, thus influencing its antioxidant activity. The effects
of stearic acid (SA) and glucose binding on HSA structural changes and thiol group content and
reactivity were monitored by fluoroscopy and the Ellman method during a 14-day incubation in
molar ratios to HSA that mimic pathophysiological conditions. Upon incubation with 5 mM glucose,
HSA glycation was the same as HSA without it, in three different HSA:SA molar ratios (HSA:SA-
1:1-2-4). The protective effect of SA on the antioxidant property of HSA under different glucose
regimes (5-10-20 mM) was significantly affected by molar ratios of HSA:SA. Thiol reactivity was fully
restored with 5–20 mM glucose at a 1:1 HSA:SA ratio, while the highest thiol content recovery was in
pathological glucose regimes at a 1:1 HSA:SA ratio. The SA affinity for HSA increased significantly
(1.5- and 1.3-fold, p < 0.01) with 5 and 10 mM glucose compared to the control. These results deepen
the knowledge about the possible regulation of the antioxidant role of HSA in diabetes and other
pathophysiological conditions and enable the design of future HSA-drug studies which, in turn, is
important for clinicians when designing information-based treatments.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Thiol Group Reactivity and the Antioxidant Property of Human Serum Albumin Are Controlled by the Joint Action of Fatty Acids and Glucose Binding",
volume = "5",
pages = "2335",
doi = "10.3390/ijms25042335"
}

Uzelac, T. N., Smiljanić, K., Takić, M., Sarac, I., Petovic-Oggiano, G., Nikolić, M.,& Jovanović, V. B.. (2024). The Thiol Group Reactivity and the Antioxidant Property of Human Serum Albumin Are Controlled by the Joint Action of Fatty Acids and Glucose Binding. in International Journal of Molecular Sciences
MDPI., 5, 2335.
https://doi.org/10.3390/ijms25042335

Uzelac TN, Smiljanić K, Takić M, Sarac I, Petovic-Oggiano G, Nikolić M, Jovanović VB. The Thiol Group Reactivity and the Antioxidant Property of Human Serum Albumin Are Controlled by the Joint Action of Fatty Acids and Glucose Binding. in International Journal of Molecular Sciences. 2024;5:2335.
doi:10.3390/ijms25042335 .

Uzelac, Tamara N., Smiljanić, Katarina, Takić, Marija, Sarac, Ivana, Petovic-Oggiano, Gordana, Nikolić, Milan, Jovanović, Vesna B., "The Thiol Group Reactivity and the Antioxidant Property of Human Serum Albumin Are Controlled by the Joint Action of Fatty Acids and Glucose Binding" in International Journal of Molecular Sciences, 5 (2024):2335,
https://doi.org/10.3390/ijms25042335 . .

TY  - JOUR
AU  - Mladenović Stokanić, Maja
AU  - Simović, Ana
AU  - Jovanović, Vesna B.
AU  - Radomirović, Mirjana
AU  - Udovićki, Božidar
AU  - Krstić Ristivojević, Maja
AU  - Đukić, Teodora
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - Sabljić, Ljiljana
AU  - Lukić, Ivana
AU  - Kovačević, Ana
AU  - Cujić, Danica
AU  - Gnjatović, Marija
AU  - Smiljanić, Katarina
AU  - Stojadinović, Marija
AU  - Radosavljević, Jelena
AU  - Stanić-Vučinić, Dragana
AU  - Stojanović, Marijana
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6436
AB  - In this study, a cost-effective sandwich ELISA test, based on polyclonal antibodies, for routine quantification SARS-CoV-2 nucleocapsid (N) protein was developed. The recombinant N protein was produced and used for the production of mice and rabbit antisera. Polyclonal N protein-specific antibodies served as capture and detection antibodies. The prototype ELISA has LOD 0.93 ng/mL and LOQ 5.3 ng/mL, with a linear range of 1.52–48.83 ng/mL. N protein heat pretreatment (56 °C, 1 h) decreased, while pretreatment with 1% Triton X-100 increased analytical ELISA sensitivity. The diagnostic specificity of ELISA was 100% (95% CI, 91.19–100.00%) and sensitivity was 52.94% (95% CI, 35.13–70.22%) compared to rtRT-PCR (Ct < 40). Profoundly higher sensitivity was obtained using patient samples mostly containing Wuhan-similar variants (Wuhan, alpha, and delta), 62.50% (95% CI, 40.59 to 81.20%), in comparison to samples mostly containing Wuhan-distant variants (Omicron) 30.00% (6.67–65.25%). The developed product has relatively high diagnostic sensitivity in relation to its analytical sensitivity due to the usage of polyclonal antibodies from two species, providing a wide repertoire of antibodies against multiple N protein epitopes. Moreover, the fast, simple, and inexpensive production of polyclonal antibodies, as the most expensive assay components, would result in affordable antigen tests.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species
VL  - 25
IS  - 1
SP  - 333
DO  - 10.3390/ijms25010333
ER  - 

@article{
author = "Mladenović Stokanić, Maja and Simović, Ana and Jovanović, Vesna B. and Radomirović, Mirjana and Udovićki, Božidar and Krstić Ristivojević, Maja and Đukić, Teodora and Vasović, Tamara and Aćimović, Jelena and Sabljić, Ljiljana and Lukić, Ivana and Kovačević, Ana and Cujić, Danica and Gnjatović, Marija and Smiljanić, Katarina and Stojadinović, Marija and Radosavljević, Jelena and Stanić-Vučinić, Dragana and Stojanović, Marijana and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "In this study, a cost-effective sandwich ELISA test, based on polyclonal antibodies, for routine quantification SARS-CoV-2 nucleocapsid (N) protein was developed. The recombinant N protein was produced and used for the production of mice and rabbit antisera. Polyclonal N protein-specific antibodies served as capture and detection antibodies. The prototype ELISA has LOD 0.93 ng/mL and LOQ 5.3 ng/mL, with a linear range of 1.52–48.83 ng/mL. N protein heat pretreatment (56 °C, 1 h) decreased, while pretreatment with 1% Triton X-100 increased analytical ELISA sensitivity. The diagnostic specificity of ELISA was 100% (95% CI, 91.19–100.00%) and sensitivity was 52.94% (95% CI, 35.13–70.22%) compared to rtRT-PCR (Ct < 40). Profoundly higher sensitivity was obtained using patient samples mostly containing Wuhan-similar variants (Wuhan, alpha, and delta), 62.50% (95% CI, 40.59 to 81.20%), in comparison to samples mostly containing Wuhan-distant variants (Omicron) 30.00% (6.67–65.25%). The developed product has relatively high diagnostic sensitivity in relation to its analytical sensitivity due to the usage of polyclonal antibodies from two species, providing a wide repertoire of antibodies against multiple N protein epitopes. Moreover, the fast, simple, and inexpensive production of polyclonal antibodies, as the most expensive assay components, would result in affordable antigen tests.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species",
volume = "25",
number = "1",
pages = "333",
doi = "10.3390/ijms25010333"
}

Mladenović Stokanić, M., Simović, A., Jovanović, V. B., Radomirović, M., Udovićki, B., Krstić Ristivojević, M., Đukić, T., Vasović, T., Aćimović, J., Sabljić, L., Lukić, I., Kovačević, A., Cujić, D., Gnjatović, M., Smiljanić, K., Stojadinović, M., Radosavljević, J., Stanić-Vučinić, D., Stojanović, M., Rajković, A.,& Ćirković-Veličković, T.. (2023). Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species. in International Journal of Molecular Sciences
MDPI., 25(1), 333.
https://doi.org/10.3390/ijms25010333

Mladenović Stokanić M, Simović A, Jovanović VB, Radomirović M, Udovićki B, Krstić Ristivojević M, Đukić T, Vasović T, Aćimović J, Sabljić L, Lukić I, Kovačević A, Cujić D, Gnjatović M, Smiljanić K, Stojadinović M, Radosavljević J, Stanić-Vučinić D, Stojanović M, Rajković A, Ćirković-Veličković T. Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species. in International Journal of Molecular Sciences. 2023;25(1):333.
doi:10.3390/ijms25010333 .

Mladenović Stokanić, Maja, Simović, Ana, Jovanović, Vesna B., Radomirović, Mirjana, Udovićki, Božidar, Krstić Ristivojević, Maja, Đukić, Teodora, Vasović, Tamara, Aćimović, Jelena, Sabljić, Ljiljana, Lukić, Ivana, Kovačević, Ana, Cujić, Danica, Gnjatović, Marija, Smiljanić, Katarina, Stojadinović, Marija, Radosavljević, Jelena, Stanić-Vučinić, Dragana, Stojanović, Marijana, Rajković, Andreja, Ćirković-Veličković, Tanja, "Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species" in International Journal of Molecular Sciences, 25, no. 1 (2023):333,
https://doi.org/10.3390/ijms25010333 . .

TY  - JOUR
AU  - de Guzman, Maria Krishna
AU  - Anđelković, Mirjana
AU  - Jovanović, Vesna B.
AU  - Jung, Jaehak
AU  - Kim, Juyang
AU  - Dailey, Lea Ann
AU  - Rajković, Andreja
AU  - De Meulenaer, Bruno
AU  - Ćirković-Veličković, Tanja
PY  - 2022
UR  - http://hdl.handle.net/1854/LU-8759702
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5420
AB  - The accumulation of microplastics in marine organisms is an emerging concern. Due to trophic transfer, the
safety of seafood is under investigation in view of the potential negative effects of microplastics on human health.
In this study, market samples of Manila clams (Ruditapes philippinarum) from South Korea were segregated into
two groups of considerably different size (p < 0.05), namely small clams with shell length of 40.69 ± 3.97 mm,
and large clams of shell length 51.19 ± 2.86 mm. Comparative profiling of the number, size, shape, and polymer
type of microplastics were performed using μFTIR imaging and Nile red staining. Overall, μFTIR detected only
1559 microplastics while 1996 microplastics were counted based on staining from 61 Manila clams (30 small and
31 large), leading to an overestimation of 18 to 75 %. Comparable microplastics concentration, based on μFTIR,
were observed at 2.70 ± 1.66 MP/g or 15.64 ± 9.25 MP/individual for the small samples, and 3.65 ± 1.59 MP/g
or 41.63 ± 16.90 MP/individual for the large ones (p > 0.05). Particle diameters of 20–100 μm was the most
dominant, accounting for 44.6 % and 46.5 % of all microplastics from the small and large groups, respectively.
Particles, with a circularity (resemblance to a circle) value between 0.6 and 1.0, were the most prevalent, fol-
lowed by fragments and fibers. At least 50 % of microplastics from the small and large samples were polystyrene,
making it the most abundant polymer type. Despite the substantial difference in the size of the animals, only a
weak to moderate correlation was observed between microplastics content and the physical attributes of the
clams such as shell length and weight, (soft) tissue weight, and total weight (Spearman's coefficient < 0.5). The
estimated intake of microplastics by the Korean population was 1232 MP/person/year via small clams, 1663 MP/
person/year via large clams, and 1489 MP/person/year via clams independent of size.
PB  - Elsevier
T2  - Marine Pollution Bulletin
T1  - Comparative profiling and exposure assessment of microplastics in differently sized Manila clams from South Korea by μFTIR and Nile Red staining
VL  - 181
SP  - 113846
DO  - 10.1016/j.marpolbul.2022.113846
ER  - 

@article{
author = "de Guzman, Maria Krishna and Anđelković, Mirjana and Jovanović, Vesna B. and Jung, Jaehak and Kim, Juyang and Dailey, Lea Ann and Rajković, Andreja and De Meulenaer, Bruno and Ćirković-Veličković, Tanja",
year = "2022",
abstract = "The accumulation of microplastics in marine organisms is an emerging concern. Due to trophic transfer, the
safety of seafood is under investigation in view of the potential negative effects of microplastics on human health.
In this study, market samples of Manila clams (Ruditapes philippinarum) from South Korea were segregated into
two groups of considerably different size (p < 0.05), namely small clams with shell length of 40.69 ± 3.97 mm,
and large clams of shell length 51.19 ± 2.86 mm. Comparative profiling of the number, size, shape, and polymer
type of microplastics were performed using μFTIR imaging and Nile red staining. Overall, μFTIR detected only
1559 microplastics while 1996 microplastics were counted based on staining from 61 Manila clams (30 small and
31 large), leading to an overestimation of 18 to 75 %. Comparable microplastics concentration, based on μFTIR,
were observed at 2.70 ± 1.66 MP/g or 15.64 ± 9.25 MP/individual for the small samples, and 3.65 ± 1.59 MP/g
or 41.63 ± 16.90 MP/individual for the large ones (p > 0.05). Particle diameters of 20–100 μm was the most
dominant, accounting for 44.6 % and 46.5 % of all microplastics from the small and large groups, respectively.
Particles, with a circularity (resemblance to a circle) value between 0.6 and 1.0, were the most prevalent, fol-
lowed by fragments and fibers. At least 50 % of microplastics from the small and large samples were polystyrene,
making it the most abundant polymer type. Despite the substantial difference in the size of the animals, only a
weak to moderate correlation was observed between microplastics content and the physical attributes of the
clams such as shell length and weight, (soft) tissue weight, and total weight (Spearman's coefficient < 0.5). The
estimated intake of microplastics by the Korean population was 1232 MP/person/year via small clams, 1663 MP/
person/year via large clams, and 1489 MP/person/year via clams independent of size.",
publisher = "Elsevier",
journal = "Marine Pollution Bulletin",
title = "Comparative profiling and exposure assessment of microplastics in differently sized Manila clams from South Korea by μFTIR and Nile Red staining",
volume = "181",
pages = "113846",
doi = "10.1016/j.marpolbul.2022.113846"
}

de Guzman, M. K., Anđelković, M., Jovanović, V. B., Jung, J., Kim, J., Dailey, L. A., Rajković, A., De Meulenaer, B.,& Ćirković-Veličković, T.. (2022). Comparative profiling and exposure assessment of microplastics in differently sized Manila clams from South Korea by μFTIR and Nile Red staining. in Marine Pollution Bulletin
Elsevier., 181, 113846.
https://doi.org/10.1016/j.marpolbul.2022.113846

de Guzman MK, Anđelković M, Jovanović VB, Jung J, Kim J, Dailey LA, Rajković A, De Meulenaer B, Ćirković-Veličković T. Comparative profiling and exposure assessment of microplastics in differently sized Manila clams from South Korea by μFTIR and Nile Red staining. in Marine Pollution Bulletin. 2022;181:113846.
doi:10.1016/j.marpolbul.2022.113846 .

de Guzman, Maria Krishna, Anđelković, Mirjana, Jovanović, Vesna B., Jung, Jaehak, Kim, Juyang, Dailey, Lea Ann, Rajković, Andreja, De Meulenaer, Bruno, Ćirković-Veličković, Tanja, "Comparative profiling and exposure assessment of microplastics in differently sized Manila clams from South Korea by μFTIR and Nile Red staining" in Marine Pollution Bulletin, 181 (2022):113846,
https://doi.org/10.1016/j.marpolbul.2022.113846 . .

TY  - CONF
AU  - Ćirković-Veličković, Tanja
AU  - Radomirović, Mirjana Ž.
AU  - Simović, Ana
AU  - Jovanović, Vesna B.
AU  - Ćujić, Danica R.
AU  - Gnjatović, Marija Lj.
AU  - Stojanović, Marijana
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5777
AB  - RT-PCR remains the primary method of diagnosing SARS-CoV-2. Serological diagnosis of COVID-19 is simple and does not require complex techniques and equipment, rendering it suitable for rapid detection and massive screening. However, serological tests cannot confirm SARS-CoV-2, and results will be false negative when antibody concentrations fall below detection limit. Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) infection. Antigen-based tests show high specificity, but sensitivity is relatively low. For development and further improvement of both serological and antigen-based tests, availability of recombinantly producted SARS CoV-2 antigens is needed and during the periods of high demend in the pandemic, it was a limited factor both for research and diagnostic tests production by national producers of tests. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of beta-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Application of serological ELISA for seropositivity detection in 200 allergic children in comparison to non-allergic did not show a bias towards any of the tested groups.  Furthermore, we have optimized production of rfNP at a large scale to raise specific antibodies to N-protein in rabbit and mice and develop an in house ELISA for quantification of N protein in biological fluids. Test was clinically validated in 200 PCR positive patients and showed a sensitivity of around 50%.
C3  - International Congress on Molecular Immunology and Allergology (IMAC-2022), Moscow, December 1-3, 2022
T1  - SARS CoV-2 nucleocapsid-based diagnostic tests and serological response in allergic children
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5777
ER  - 

@conference{
author = "Ćirković-Veličković, Tanja and Radomirović, Mirjana Ž. and Simović, Ana and Jovanović, Vesna B. and Ćujić, Danica R. and Gnjatović, Marija Lj. and Stojanović, Marijana",
year = "2022",
abstract = "RT-PCR remains the primary method of diagnosing SARS-CoV-2. Serological diagnosis of COVID-19 is simple and does not require complex techniques and equipment, rendering it suitable for rapid detection and massive screening. However, serological tests cannot confirm SARS-CoV-2, and results will be false negative when antibody concentrations fall below detection limit. Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) infection. Antigen-based tests show high specificity, but sensitivity is relatively low. For development and further improvement of both serological and antigen-based tests, availability of recombinantly producted SARS CoV-2 antigens is needed and during the periods of high demend in the pandemic, it was a limited factor both for research and diagnostic tests production by national producers of tests. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of beta-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Application of serological ELISA for seropositivity detection in 200 allergic children in comparison to non-allergic did not show a bias towards any of the tested groups.  Furthermore, we have optimized production of rfNP at a large scale to raise specific antibodies to N-protein in rabbit and mice and develop an in house ELISA for quantification of N protein in biological fluids. Test was clinically validated in 200 PCR positive patients and showed a sensitivity of around 50%.",
journal = "International Congress on Molecular Immunology and Allergology (IMAC-2022), Moscow, December 1-3, 2022",
title = "SARS CoV-2 nucleocapsid-based diagnostic tests and serological response in allergic children",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5777"
}

Ćirković-Veličković, T., Radomirović, M. Ž., Simović, A., Jovanović, V. B., Ćujić, D. R., Gnjatović, M. Lj.,& Stojanović, M.. (2022). SARS CoV-2 nucleocapsid-based diagnostic tests and serological response in allergic children. in International Congress on Molecular Immunology and Allergology (IMAC-2022), Moscow, December 1-3, 2022.
https://hdl.handle.net/21.15107/rcub_cherry_5777

Ćirković-Veličković T, Radomirović MŽ, Simović A, Jovanović VB, Ćujić DR, Gnjatović ML, Stojanović M. SARS CoV-2 nucleocapsid-based diagnostic tests and serological response in allergic children. in International Congress on Molecular Immunology and Allergology (IMAC-2022), Moscow, December 1-3, 2022. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5777 .

Ćirković-Veličković, Tanja, Radomirović, Mirjana Ž., Simović, Ana, Jovanović, Vesna B., Ćujić, Danica R., Gnjatović, Marija Lj., Stojanović, Marijana, "SARS CoV-2 nucleocapsid-based diagnostic tests and serological response in allergic children" in International Congress on Molecular Immunology and Allergology (IMAC-2022), Moscow, December 1-3, 2022 (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5777 .

TY  - CONF
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Đukić, Teodora
AU  - Vasović, Tamara
AU  - Jovanović, Vesna B.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5716
C3  - Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021
T1  - Effects of lysin's and arginige's modifications on trypsin proteolytic efficacy imposed before and after the peanut roasting
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5716
ER  - 

@conference{
author = "Smiljanić, Katarina and Prodić, Ivana and Đukić, Teodora and Vasović, Tamara and Jovanović, Vesna B. and Ćirković-Veličković, Tanja",
year = "2021",
journal = "Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021",
title = "Effects of lysin's and arginige's modifications on trypsin proteolytic efficacy imposed before and after the peanut roasting",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5716"
}

Smiljanić, K., Prodić, I., Đukić, T., Vasović, T., Jovanović, V. B.,& Ćirković-Veličković, T.. (2021). Effects of lysin's and arginige's modifications on trypsin proteolytic efficacy imposed before and after the peanut roasting. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 71-71.
https://hdl.handle.net/21.15107/rcub_cherry_5716

Smiljanić K, Prodić I, Đukić T, Vasović T, Jovanović VB, Ćirković-Veličković T. Effects of lysin's and arginige's modifications on trypsin proteolytic efficacy imposed before and after the peanut roasting. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021. 2021;:71-71.
https://hdl.handle.net/21.15107/rcub_cherry_5716 .

Smiljanić, Katarina, Prodić, Ivana, Đukić, Teodora, Vasović, Tamara, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "Effects of lysin's and arginige's modifications on trypsin proteolytic efficacy imposed before and after the peanut roasting" in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021 (2021):71-71,
https://hdl.handle.net/21.15107/rcub_cherry_5716 .

TY  - CONF
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Đukić, Teodora
AU  - Vasović, Tamara
AU  - Jovanović, Vesna B.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5773
AB  - Background: We have undertaken study on our porcine-derived trypsin generated
proteomic data of the major peanut allergen Ara h 1 from the raw and roasted peanut, to
assess possible facilitating/hindrance effects on trypsin digestion efficacy caused by post-
translational and chemical modifications (PTMs) positioned on K/R residues. If potential
hindrance effects caused by PTMs are observed with porcine trypsin, then they can be just
augmented and more pronounced within human intestinal digestion. The logic for such
reasoning is in inferior performance of human trypsin compared to porcine-derived used in
proteomic digestion protocols, also the lower trypsin-to-sample ratio and much shorter
digestion times, even though gastric digestion precedes and trypsin is not the sole digestive
enzyme.
Methods: Novel method was developed to decipher outcomes at scissile bonds using PEAKS
Studio-X+ in reassessment of high-resolution tandem mass spectrometry data on 18h-long
trypsin digestion protocol.
Results: In eight modified K/R residues involving methylation, dihydroxy and formylation,
differences in extent of miscleavage between modified and unmodified peptides, were
significantly higher (>10%) in modified peptides.
Conclusion: It is important to elucidate impact of modifications on trypsin digestion
performance, but also on other proteases involved in digestion process due to possible
effects on allergenicity of food proteins/peptides.
PB  - INFOGEST Cost action, INRAE, Teagasc LTD.
C3  - Virtual International Conference on Food Digestion 6th and 7th May, 2021
T1  - Trypsin as a proteomic probe to assess food protein digestibility in relation to post- translational modifications
SP  - 18
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5773
ER  - 

@conference{
author = "Smiljanić, Katarina and Prodić, Ivana and Đukić, Teodora and Vasović, Tamara and Jovanović, Vesna B. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Background: We have undertaken study on our porcine-derived trypsin generated
proteomic data of the major peanut allergen Ara h 1 from the raw and roasted peanut, to
assess possible facilitating/hindrance effects on trypsin digestion efficacy caused by post-
translational and chemical modifications (PTMs) positioned on K/R residues. If potential
hindrance effects caused by PTMs are observed with porcine trypsin, then they can be just
augmented and more pronounced within human intestinal digestion. The logic for such
reasoning is in inferior performance of human trypsin compared to porcine-derived used in
proteomic digestion protocols, also the lower trypsin-to-sample ratio and much shorter
digestion times, even though gastric digestion precedes and trypsin is not the sole digestive
enzyme.
Methods: Novel method was developed to decipher outcomes at scissile bonds using PEAKS
Studio-X+ in reassessment of high-resolution tandem mass spectrometry data on 18h-long
trypsin digestion protocol.
Results: In eight modified K/R residues involving methylation, dihydroxy and formylation,
differences in extent of miscleavage between modified and unmodified peptides, were
significantly higher (>10%) in modified peptides.
Conclusion: It is important to elucidate impact of modifications on trypsin digestion
performance, but also on other proteases involved in digestion process due to possible
effects on allergenicity of food proteins/peptides.",
publisher = "INFOGEST Cost action, INRAE, Teagasc LTD.",
journal = "Virtual International Conference on Food Digestion 6th and 7th May, 2021",
title = "Trypsin as a proteomic probe to assess food protein digestibility in relation to post- translational modifications",
pages = "18-18",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5773"
}

Smiljanić, K., Prodić, I., Đukić, T., Vasović, T., Jovanović, V. B.,& Ćirković-Veličković, T.. (2021). Trypsin as a proteomic probe to assess food protein digestibility in relation to post- translational modifications. in Virtual International Conference on Food Digestion 6th and 7th May, 2021
INFOGEST Cost action, INRAE, Teagasc LTD.., 18-18.
https://hdl.handle.net/21.15107/rcub_cherry_5773

Smiljanić K, Prodić I, Đukić T, Vasović T, Jovanović VB, Ćirković-Veličković T. Trypsin as a proteomic probe to assess food protein digestibility in relation to post- translational modifications. in Virtual International Conference on Food Digestion 6th and 7th May, 2021. 2021;:18-18.
https://hdl.handle.net/21.15107/rcub_cherry_5773 .

Smiljanić, Katarina, Prodić, Ivana, Đukić, Teodora, Vasović, Tamara, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "Trypsin as a proteomic probe to assess food protein digestibility in relation to post- translational modifications" in Virtual International Conference on Food Digestion 6th and 7th May, 2021 (2021):18-18,
https://hdl.handle.net/21.15107/rcub_cherry_5773 .

TY  - CONF
AU  - Smiljanić, Katarina
AU  - Đukić, Teodora
AU  - Vasović, Tamara
AU  - Prodić, Ivana
AU  - Jovanović, Vesna B.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5763
AB  - Post-translational modifications (PTMs) occur in many forms, and broadly influence protein behavior.
High-resolution tandem mass spectrometry coupled with engines for the identification of unspecified
PTMs, is a first-choice method for their global mapping. The significance of untargeted, in-depth PTM
profiling of various proteomes and its method development, just emerged, in contrast to proteomic studies
dealing with few selected static and dynamic modifications. In 2018, we have finalized our first study of
comprehensive analysis of multiple polluted and environmentally preserved Timothy grass pollen
samples that included novel method of relative quantification of proteins expressions and of open,
quantitative PTM profiling. It was among the first ones that has appeared in the proteomics field. In
addition to this, relative quantitative PTMs profiling of the raw and roasted peanut allergens was
completed in 2020, including validation of the method by specific antibodies. This peanut PTM study was
inspiration for the idea that porcine trypsin used in proteomic experiments can serve as a probe to
decipher differences in scissile bond hydrolysis caused by PTMs, with the steric and/or charge changes
causing possible hindrance or facilitation to its activity. We further hypothesized that effects observed
would be even more pronounced with human trypsin, since it is less efficient compared to the porcine
counterpart. Finally, we developed dual manual method to reassess our data of the major peanut allergen
Ara h 1 from the raw and roasted peanut, to confirm or rule out the first hypothesis (do PTMs positioned
on lysine/arginine residues facilitate or hinder porcine trypsin digestion efficacy). We believe that this
topic is relevant from the aspect of human gastrointestinal digestion of food allergens and PTMs
introduced by food processing. For further advancement in this area, novel algorithms based on deep
machine learning, capable of reporting cleavage and miscleavage events separately within unmodified
and modified part of protein sequences are warranted.
PB  - Univerzitet u Beogradu - Hemijski fakultet
C3  - Book of Abstracts of the Final 3rd Workshop FoodenTwin 2021
T1  - Methods Development for Protein and Modifications Profiling
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5763
ER  - 

@conference{
author = "Smiljanić, Katarina and Đukić, Teodora and Vasović, Tamara and Prodić, Ivana and Jovanović, Vesna B. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Post-translational modifications (PTMs) occur in many forms, and broadly influence protein behavior.
High-resolution tandem mass spectrometry coupled with engines for the identification of unspecified
PTMs, is a first-choice method for their global mapping. The significance of untargeted, in-depth PTM
profiling of various proteomes and its method development, just emerged, in contrast to proteomic studies
dealing with few selected static and dynamic modifications. In 2018, we have finalized our first study of
comprehensive analysis of multiple polluted and environmentally preserved Timothy grass pollen
samples that included novel method of relative quantification of proteins expressions and of open,
quantitative PTM profiling. It was among the first ones that has appeared in the proteomics field. In
addition to this, relative quantitative PTMs profiling of the raw and roasted peanut allergens was
completed in 2020, including validation of the method by specific antibodies. This peanut PTM study was
inspiration for the idea that porcine trypsin used in proteomic experiments can serve as a probe to
decipher differences in scissile bond hydrolysis caused by PTMs, with the steric and/or charge changes
causing possible hindrance or facilitation to its activity. We further hypothesized that effects observed
would be even more pronounced with human trypsin, since it is less efficient compared to the porcine
counterpart. Finally, we developed dual manual method to reassess our data of the major peanut allergen
Ara h 1 from the raw and roasted peanut, to confirm or rule out the first hypothesis (do PTMs positioned
on lysine/arginine residues facilitate or hinder porcine trypsin digestion efficacy). We believe that this
topic is relevant from the aspect of human gastrointestinal digestion of food allergens and PTMs
introduced by food processing. For further advancement in this area, novel algorithms based on deep
machine learning, capable of reporting cleavage and miscleavage events separately within unmodified
and modified part of protein sequences are warranted.",
publisher = "Univerzitet u Beogradu - Hemijski fakultet",
journal = "Book of Abstracts of the Final 3rd Workshop FoodenTwin 2021",
title = "Methods Development for Protein and Modifications Profiling",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5763"
}

Smiljanić, K., Đukić, T., Vasović, T., Prodić, I., Jovanović, V. B.,& Ćirković-Veličković, T.. (2021). Methods Development for Protein and Modifications Profiling. in Book of Abstracts of the Final 3rd Workshop FoodenTwin 2021
Univerzitet u Beogradu - Hemijski fakultet., 11-11.
https://hdl.handle.net/21.15107/rcub_cherry_5763

Smiljanić K, Đukić T, Vasović T, Prodić I, Jovanović VB, Ćirković-Veličković T. Methods Development for Protein and Modifications Profiling. in Book of Abstracts of the Final 3rd Workshop FoodenTwin 2021. 2021;:11-11.
https://hdl.handle.net/21.15107/rcub_cherry_5763 .

Smiljanić, Katarina, Đukić, Teodora, Vasović, Tamara, Prodić, Ivana, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "Methods Development for Protein and Modifications Profiling" in Book of Abstracts of the Final 3rd Workshop FoodenTwin 2021 (2021):11-11,
https://hdl.handle.net/21.15107/rcub_cherry_5763 .

TY  - CONF
AU  - Đukić, Teodora
AU  - Vasović, Tamara
AU  - Mladenović, Maja
AU  - Jovanović, Vesna B.
AU  - Smiljanić, Katarina
AU  - Radosavljević, Jelena
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5774
AB  - Nucleocapsid (N) protein is the most abundant SARS-CoV-2 virus derived protein and strong immunogen which can be used as a component of the immunological tests for the diagnosis of SARS-CoV-2 infection. Recombinant fragment of N-protein (58–419 aa) was expressed in E. coli in a soluble form using developed optimized protocol of expression (16-18h, 37 °C, 0.4 mM IPTG). After lysis of cells, N-protein from soluble fraction of lysate was purified using optimized protocol for purification by immobilized metal affinity chromatography on Ni-Sepharose in two repeated steps under different elution conditions. Obtained fraction of N-protein after the second chromatography was desalted and concentrated using phosphate buffer solution and ultrafiltration. The purity of isolated N-protein was deterrmined by SDS PAGE, while high resolution mass spectrometry was used for its characterization. Isolated N-protein was over 90% purity and identified as the most intense and abundant protein fragment, with PEAKS PTM score of 508 and sequence coverage of over 70%, including 173 unique peptides.
PB  - Belgrade : Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference
with international participation “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24th September 2021
T1  - Optimization of expression, purification and HRMS characterization of recombinant N-protein fragment from SARS-CoV-2
SP  - 108
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5774
ER  - 

@conference{
author = "Đukić, Teodora and Vasović, Tamara and Mladenović, Maja and Jovanović, Vesna B. and Smiljanić, Katarina and Radosavljević, Jelena and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Nucleocapsid (N) protein is the most abundant SARS-CoV-2 virus derived protein and strong immunogen which can be used as a component of the immunological tests for the diagnosis of SARS-CoV-2 infection. Recombinant fragment of N-protein (58–419 aa) was expressed in E. coli in a soluble form using developed optimized protocol of expression (16-18h, 37 °C, 0.4 mM IPTG). After lysis of cells, N-protein from soluble fraction of lysate was purified using optimized protocol for purification by immobilized metal affinity chromatography on Ni-Sepharose in two repeated steps under different elution conditions. Obtained fraction of N-protein after the second chromatography was desalted and concentrated using phosphate buffer solution and ultrafiltration. The purity of isolated N-protein was deterrmined by SDS PAGE, while high resolution mass spectrometry was used for its characterization. Isolated N-protein was over 90% purity and identified as the most intense and abundant protein fragment, with PEAKS PTM score of 508 and sequence coverage of over 70%, including 173 unique peptides.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference
with international participation “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24th September 2021",
title = "Optimization of expression, purification and HRMS characterization of recombinant N-protein fragment from SARS-CoV-2",
pages = "108-108",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5774"
}

Đukić, T., Vasović, T., Mladenović, M., Jovanović, V. B., Smiljanić, K., Radosavljević, J., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2021). Optimization of expression, purification and HRMS characterization of recombinant N-protein fragment from SARS-CoV-2. in Serbian Biochemical Society Tenth Conference
with international participation “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24th September 2021
Belgrade : Serbian Biochemical Society., 108-108.
https://hdl.handle.net/21.15107/rcub_cherry_5774

Đukić T, Vasović T, Mladenović M, Jovanović VB, Smiljanić K, Radosavljević J, Stanić-Vučinić D, Ćirković-Veličković T. Optimization of expression, purification and HRMS characterization of recombinant N-protein fragment from SARS-CoV-2. in Serbian Biochemical Society Tenth Conference
with international participation “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24th September 2021. 2021;:108-108.
https://hdl.handle.net/21.15107/rcub_cherry_5774 .

Đukić, Teodora, Vasović, Tamara, Mladenović, Maja, Jovanović, Vesna B., Smiljanić, Katarina, Radosavljević, Jelena, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Optimization of expression, purification and HRMS characterization of recombinant N-protein fragment from SARS-CoV-2" in Serbian Biochemical Society Tenth Conference
with international participation “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24th September 2021 (2021):108-108,
https://hdl.handle.net/21.15107/rcub_cherry_5774 .

TY  - CONF
AU  - Jovanović, Vesna B.
AU  - Smiljanić, Katarina
AU  - Lujić, Tamara
AU  - Đukić, Teodora
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5736
AB  - Edible insects are recommended as a future food because of many reasons. The nutritional value of edible insects is one of criteria for the selection of seven most promising species among which is Tenebrio molitor. The objective of this study was to examine the effects of different flour Tenebrio molitor. Twelve different extraction conditions were set up in which three parameters were combined: pH extraction solutions (6, 8 and 12.5), temperature (30 °C and 60 °C), and ultrasound (US). Shotgun proteomics of trypsin digests profiled protein isolates. The highest protein yield was in extractions at pH 12.5. The temperature elevation and US application significantly increased the yield of isolated proteins at pH 12.5 but their solubility at the pH 7.4 was lower compared to isolates at pH 6 and 8. 1D-SDS-PAGE showed marked differences in protein profiles on various extraction conditions, with highest number of the distinctive bands at pH 8 at 30 °C. Shotgun proteomics showed that extraction condition at pH 12.5, on 30 °C has the highest numbers of different proteins, however, among the top 20 abundant proteins are chitin-associated proteins, allergens and proteinases, while at pH 8 these proteins are not enriched. Highly basic extraction significantly contributes to protein hydrolysis while application of US contributes to the protein cross-linking and this effect is more prominent at high temperatures.
C3  - Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021
T1  - Effects of extraction conditions on proteins' profiles of Tenebrio molitor
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5736
ER  - 

@conference{
author = "Jovanović, Vesna B. and Smiljanić, Katarina and Lujić, Tamara and Đukić, Teodora and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Edible insects are recommended as a future food because of many reasons. The nutritional value of edible insects is one of criteria for the selection of seven most promising species among which is Tenebrio molitor. The objective of this study was to examine the effects of different flour Tenebrio molitor. Twelve different extraction conditions were set up in which three parameters were combined: pH extraction solutions (6, 8 and 12.5), temperature (30 °C and 60 °C), and ultrasound (US). Shotgun proteomics of trypsin digests profiled protein isolates. The highest protein yield was in extractions at pH 12.5. The temperature elevation and US application significantly increased the yield of isolated proteins at pH 12.5 but their solubility at the pH 7.4 was lower compared to isolates at pH 6 and 8. 1D-SDS-PAGE showed marked differences in protein profiles on various extraction conditions, with highest number of the distinctive bands at pH 8 at 30 °C. Shotgun proteomics showed that extraction condition at pH 12.5, on 30 °C has the highest numbers of different proteins, however, among the top 20 abundant proteins are chitin-associated proteins, allergens and proteinases, while at pH 8 these proteins are not enriched. Highly basic extraction significantly contributes to protein hydrolysis while application of US contributes to the protein cross-linking and this effect is more prominent at high temperatures.",
journal = "Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021",
title = "Effects of extraction conditions on proteins' profiles of Tenebrio molitor",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5736"
}

Jovanović, V. B., Smiljanić, K., Lujić, T., Đukić, T.,& Ćirković-Veličković, T.. (2021). Effects of extraction conditions on proteins' profiles of Tenebrio molitor. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 53-53.
https://hdl.handle.net/21.15107/rcub_cherry_5736

Jovanović VB, Smiljanić K, Lujić T, Đukić T, Ćirković-Veličković T. Effects of extraction conditions on proteins' profiles of Tenebrio molitor. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021. 2021;:53-53.
https://hdl.handle.net/21.15107/rcub_cherry_5736 .

Jovanović, Vesna B., Smiljanić, Katarina, Lujić, Tamara, Đukić, Teodora, Ćirković-Veličković, Tanja, "Effects of extraction conditions on proteins' profiles of Tenebrio molitor" in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021 (2021):53-53,
https://hdl.handle.net/21.15107/rcub_cherry_5736 .

TY  - JOUR
AU  - Mutić, Jelena
AU  - Jovanović, Vesna B.
AU  - Jacxsens, Liesbeth
AU  - Tondeleir, Jannes
AU  - Ristivojević, Petar
AU  - Đurđić, Slađana Z.
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4825
AB  - Bivalves are a good source of nutrients but also a potential source of environmental contaminants, which could pose a risk for consumers. The aims of this study were: the determination of 16 elements by ICP-MS in 48 samples of five bivalve species purchased from market in Korea; the identification of elements useful for species classification using multivariate analyses; and the benefit-risk evaluation associated to the consumption of these bivalves. The highest difference among content of elements between species was found for Cd, Mn, Ni, Zn, and Fe. Partial last squares discriminant analysis revealed elements with a VIP score >1 which were considered as the most relevant for explaining certain species. As, Cd, Co, and Ni were found as taxonomical markers of V. philippinarum; Mn, Zn, Mg, and Na of A. irradians; and Cd, Ni, and Fe of M. yessoensis. These species could serve as good dietary sources of essential elements. Cd exposure by consumption of Manila clams is not representing a health risk for the Korean population; however, through consumption of Yesso scallops, 5.3% of the Korean population has a potential health risk. Removal of the digestive gland before eating will drastically reduce the amount of Cd ingested.
PB  - MDPI
T2  - Foods
T1  - Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation
VL  - 10
IS  - 11
SP  - 2690
DO  - 10.3390/foods10112690
ER  - 

@article{
author = "Mutić, Jelena and Jovanović, Vesna B. and Jacxsens, Liesbeth and Tondeleir, Jannes and Ristivojević, Petar and Đurđić, Slađana Z. and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Bivalves are a good source of nutrients but also a potential source of environmental contaminants, which could pose a risk for consumers. The aims of this study were: the determination of 16 elements by ICP-MS in 48 samples of five bivalve species purchased from market in Korea; the identification of elements useful for species classification using multivariate analyses; and the benefit-risk evaluation associated to the consumption of these bivalves. The highest difference among content of elements between species was found for Cd, Mn, Ni, Zn, and Fe. Partial last squares discriminant analysis revealed elements with a VIP score >1 which were considered as the most relevant for explaining certain species. As, Cd, Co, and Ni were found as taxonomical markers of V. philippinarum; Mn, Zn, Mg, and Na of A. irradians; and Cd, Ni, and Fe of M. yessoensis. These species could serve as good dietary sources of essential elements. Cd exposure by consumption of Manila clams is not representing a health risk for the Korean population; however, through consumption of Yesso scallops, 5.3% of the Korean population has a potential health risk. Removal of the digestive gland before eating will drastically reduce the amount of Cd ingested.",
publisher = "MDPI",
journal = "Foods",
title = "Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation",
volume = "10",
number = "11",
pages = "2690",
doi = "10.3390/foods10112690"
}

Mutić, J., Jovanović, V. B., Jacxsens, L., Tondeleir, J., Ristivojević, P., Đurđić, S. Z., Rajković, A.,& Ćirković-Veličković, T.. (2021). Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. in Foods
MDPI., 10(11), 2690.
https://doi.org/10.3390/foods10112690

Mutić J, Jovanović VB, Jacxsens L, Tondeleir J, Ristivojević P, Đurđić SZ, Rajković A, Ćirković-Veličković T. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. in Foods. 2021;10(11):2690.
doi:10.3390/foods10112690 .

Mutić, Jelena, Jovanović, Vesna B., Jacxsens, Liesbeth, Tondeleir, Jannes, Ristivojević, Petar, Đurđić, Slađana Z., Rajković, Andreja, Ćirković-Veličković, Tanja, "Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation" in Foods, 10, no. 11 (2021):2690,
https://doi.org/10.3390/foods10112690 . .

TY  - DATA
AU  - Mutić, Jelena
AU  - Jovanović, Vesna B.
AU  - Jacxsens, Liesbeth
AU  - Tondeleir, Jannes
AU  - Ristivojević, Petar
AU  - Đurđić, Slađana Z.
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4826
PB  - MDPI
T2  - Foods
T1  - Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4826
ER  - 

@misc{
author = "Mutić, Jelena and Jovanović, Vesna B. and Jacxsens, Liesbeth and Tondeleir, Jannes and Ristivojević, Petar and Đurđić, Slađana Z. and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
publisher = "MDPI",
journal = "Foods",
title = "Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4826"
}

Mutić, J., Jovanović, V. B., Jacxsens, L., Tondeleir, J., Ristivojević, P., Đurđić, S. Z., Rajković, A.,& Ćirković-Veličković, T.. (2021). Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.. in Foods
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_4826

Mutić J, Jovanović VB, Jacxsens L, Tondeleir J, Ristivojević P, Đurđić SZ, Rajković A, Ćirković-Veličković T. Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690.. in Foods. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4826 .

Mutić, Jelena, Jovanović, Vesna B., Jacxsens, Liesbeth, Tondeleir, Jannes, Ristivojević, Petar, Đurđić, Slađana Z., Rajković, Andreja, Ćirković-Veličković, Tanja, "Supplementary data for the article: Mutić, J.; Jovanović, V.; Jacxsens, L.; Tondeleir, J.; Ristivojević, P.; Djurdjić, S.; Rajković, A.; Veličković, T. Ć. Chemical Content of Five Molluscan Bivalve Species Collected from South Korea: Multivariate Study and Safety Evaluation. Foods 2021, 10 (11), 2690. https://doi.org/10.3390/foods10112690." in Foods (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4826 .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Jovanović, Vesna B.
AU  - Milojković-Opsenica, Dušanka
AU  - Park, Jihae
AU  - Rollinger, Judith M.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4844
AB  - Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.
PB  - Elsevier
T2  - Food Chemistry
T1  - Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds
VL  - 334
SP  - 127562
DO  - 10.1016/j.foodchem.2020.127562
ER  - 

@article{
author = "Ristivojević, Petar and Jovanović, Vesna B. and Milojković-Opsenica, Dušanka and Park, Jihae and Rollinger, Judith M. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds",
volume = "334",
pages = "127562",
doi = "10.1016/j.foodchem.2020.127562"
}

Ristivojević, P., Jovanović, V. B., Milojković-Opsenica, D., Park, J., Rollinger, J. M.,& Ćirković-Veličković, T.. (2021). Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry
Elsevier., 334, 127562.
https://doi.org/10.1016/j.foodchem.2020.127562

Ristivojević P, Jovanović VB, Milojković-Opsenica D, Park J, Rollinger JM, Ćirković-Veličković T. Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry. 2021;334:127562.
doi:10.1016/j.foodchem.2020.127562 .

Ristivojević, Petar, Jovanović, Vesna B., Milojković-Opsenica, Dušanka, Park, Jihae, Rollinger, Judith M., Ćirković-Veličković, Tanja, "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds" in Food Chemistry, 334 (2021):127562,
https://doi.org/10.1016/j.foodchem.2020.127562 . .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Jovanović, Vesna B.
AU  - Milojković-Opsenica, Dušanka
AU  - Park, Jihae
AU  - Rollinger, Judith M.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4844
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4864
AB  - Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.
PB  - Elsevier
T2  - Food Chemistry
T1  - Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds
VL  - 334
SP  - 127562
DO  - 10.1016/j.foodchem.2020.127562
ER  - 

@article{
author = "Ristivojević, Petar and Jovanović, Vesna B. and Milojković-Opsenica, Dušanka and Park, Jihae and Rollinger, Judith M. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds",
volume = "334",
pages = "127562",
doi = "10.1016/j.foodchem.2020.127562"
}

Ristivojević, P., Jovanović, V. B., Milojković-Opsenica, D., Park, J., Rollinger, J. M.,& Ćirković-Veličković, T.. (2021). Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry
Elsevier., 334, 127562.
https://doi.org/10.1016/j.foodchem.2020.127562

Ristivojević P, Jovanović VB, Milojković-Opsenica D, Park J, Rollinger JM, Ćirković-Veličković T. Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds. in Food Chemistry. 2021;334:127562.
doi:10.1016/j.foodchem.2020.127562 .

Ristivojević, Petar, Jovanović, Vesna B., Milojković-Opsenica, Dušanka, Park, Jihae, Rollinger, Judith M., Ćirković-Veličković, Tanja, "Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds" in Food Chemistry, 334 (2021):127562,
https://doi.org/10.1016/j.foodchem.2020.127562 . .

TY  - CONF
AU  - Mladenović, Maja
AU  - Romanyuk, Nataliya
AU  - Smiljanić, Katarina
AU  - Jovanović, Vesna B.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5738
AB  - Introduction: Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Detection of tropomyosin in seashells is a challenge because there are no specific antibodies for seashells’ tropomyosin. Our aim was to verify the presence of tropomyosin in Anadara seashells using an immunoproteomic approach and to investigate the level of cross-reactivity with shrimps.
Methods and Results: Proteins from lyophilized seashells Tegillarca granosa (TG) and Anadara broughtonii (AB) were extracted in: RIPA buffer (1% Triton X-100, 1% sodium deoxycholate, 0,1% SDS, 150 mM NaCl, 50 mM Tris-HCl, 1mM EDTA) and Rehydration buffer (7M urea, 2M thiourea, 2% CHAPS and 10mM DTT). Protein concentration of extracts was determined by Bradford assay and SDS-PAGE. The presence of tropomyosin has been supported by commercial tropomyosin standard in 1D SDS-PAGE. With 1D immunoblot, it was possible to confirm the reactivity of seashells’ tropomyosin to rabbit anti-shrimp tropomyosin polyclonal antibodies, confirming its presence. Tropomyosin’s presence was also validated with 1D immunoblot using monoclonal antibodies. 2D electrophoresis showed that most of samples’ proteins are in acidic pI range with prevalence of spots in the range 35-50kDa, and, by comparing spots to 2D immunoblot with polyclonal antibodies, it is possible to confirm tropomyosin’s presence in Anadara seashells.
Conclusions: We found that tropomyosin is present in both blood clam species. Both monoclonal and polyclonal antibodies raised against shrimp tropomyosin can detect seashells tropomyosin by immunoblot pointing to a potential antibodies cross-reactivity of allergic subjects to shrimps and seashells.
C3  - FoodEnTwin Symposium: Novel analytical approaches in food and environmental sciences, June 16-18, 2021 Belgrade, Serbia
T1  - Detection  and characterization of tropomyosin from Anadara Seashells using immunoproteomic aproach
SP  - 35
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5738
ER  - 

@conference{
author = "Mladenović, Maja and Romanyuk, Nataliya and Smiljanić, Katarina and Jovanović, Vesna B. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Introduction: Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Detection of tropomyosin in seashells is a challenge because there are no specific antibodies for seashells’ tropomyosin. Our aim was to verify the presence of tropomyosin in Anadara seashells using an immunoproteomic approach and to investigate the level of cross-reactivity with shrimps.
Methods and Results: Proteins from lyophilized seashells Tegillarca granosa (TG) and Anadara broughtonii (AB) were extracted in: RIPA buffer (1% Triton X-100, 1% sodium deoxycholate, 0,1% SDS, 150 mM NaCl, 50 mM Tris-HCl, 1mM EDTA) and Rehydration buffer (7M urea, 2M thiourea, 2% CHAPS and 10mM DTT). Protein concentration of extracts was determined by Bradford assay and SDS-PAGE. The presence of tropomyosin has been supported by commercial tropomyosin standard in 1D SDS-PAGE. With 1D immunoblot, it was possible to confirm the reactivity of seashells’ tropomyosin to rabbit anti-shrimp tropomyosin polyclonal antibodies, confirming its presence. Tropomyosin’s presence was also validated with 1D immunoblot using monoclonal antibodies. 2D electrophoresis showed that most of samples’ proteins are in acidic pI range with prevalence of spots in the range 35-50kDa, and, by comparing spots to 2D immunoblot with polyclonal antibodies, it is possible to confirm tropomyosin’s presence in Anadara seashells.
Conclusions: We found that tropomyosin is present in both blood clam species. Both monoclonal and polyclonal antibodies raised against shrimp tropomyosin can detect seashells tropomyosin by immunoblot pointing to a potential antibodies cross-reactivity of allergic subjects to shrimps and seashells.",
journal = "FoodEnTwin Symposium: Novel analytical approaches in food and environmental sciences, June 16-18, 2021 Belgrade, Serbia",
title = "Detection  and characterization of tropomyosin from Anadara Seashells using immunoproteomic aproach",
pages = "35-35",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5738"
}

Mladenović, M., Romanyuk, N., Smiljanić, K., Jovanović, V. B.,& Ćirković-Veličković, T.. (2021). Detection  and characterization of tropomyosin from Anadara Seashells using immunoproteomic aproach. in FoodEnTwin Symposium: Novel analytical approaches in food and environmental sciences, June 16-18, 2021 Belgrade, Serbia, 35-35.
https://hdl.handle.net/21.15107/rcub_cherry_5738

Mladenović M, Romanyuk N, Smiljanić K, Jovanović VB, Ćirković-Veličković T. Detection  and characterization of tropomyosin from Anadara Seashells using immunoproteomic aproach. in FoodEnTwin Symposium: Novel analytical approaches in food and environmental sciences, June 16-18, 2021 Belgrade, Serbia. 2021;:35-35.
https://hdl.handle.net/21.15107/rcub_cherry_5738 .

Mladenović, Maja, Romanyuk, Nataliya, Smiljanić, Katarina, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "Detection  and characterization of tropomyosin from Anadara Seashells using immunoproteomic aproach" in FoodEnTwin Symposium: Novel analytical approaches in food and environmental sciences, June 16-18, 2021 Belgrade, Serbia (2021):35-35,
https://hdl.handle.net/21.15107/rcub_cherry_5738 .

TY  - DATA
AU  - Ristivojević, Petar
AU  - Jovanović, Vesna B.
AU  - Milojković-Opsenica, Dušanka
AU  - Park, Jihae
AU  - Rollinger, Judith M.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4865
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4865
ER  - 

@misc{
author = "Ristivojević, Petar and Jovanović, Vesna B. and Milojković-Opsenica, Dušanka and Park, Jihae and Rollinger, Judith M. and Ćirković-Veličković, Tanja",
year = "2021",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4865"
}

Ristivojević, P., Jovanović, V. B., Milojković-Opsenica, D., Park, J., Rollinger, J. M.,& Ćirković-Veličković, T.. (2021). Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.. in Food Chemistry
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4865

Ristivojević P, Jovanović VB, Milojković-Opsenica D, Park J, Rollinger JM, Ćirković-Veličković T. Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562.. in Food Chemistry. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4865 .

Ristivojević, Petar, Jovanović, Vesna B., Milojković-Opsenica, Dušanka, Park, Jihae, Rollinger, Judith M., Ćirković-Veličković, Tanja, "Supplementary data for the article: Ristivojević, P.; Jovanović, V.; Milojković-Opsenica, D.; Park, J.; Rollinger, J. M.; Ćirković-Veličković, T. Rapid Analytical Approach for Bioprofiling Compounds with Radical Scavenging and Antimicrobial Activities from Seaweeds. Food Chemistry 2021, 334, 127562. https://doi.org/10.1016/j.foodchem.2020.127562." in Food Chemistry (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4865 .

TY  - JOUR
AU  - Nikkhah, Amin
AU  - Van Haute, Sam
AU  - Jovanović, Vesna B.
AU  - Jung, Heejung
AU  - Dewulf, Jo
AU  - Ćirković-Veličković, Tanja
AU  - Ghnimi, Sami
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4584
AB  - Because it is important to develop new sustainable sources of edible protein, insects have been recommended as a new protein source. This study applied Life Cycle Assessment (LCA) to investigate the environmental impact of small-scale edible insect production unit in South Korea. IMPACT 2002 + was applied as the baseline impact assessment (IA) methodology. The CML-IA baseline, EDIP 2003, EDP 2013, ILCD 2011 Midpoint, and ReCiPe midpoint IA methodologies were also used for LCIA methodology sensitivity analysis. The protein, fat contents, and fatty acid profile of the investigated insect (Protaetia brevitarsis seulensis larvae) were analyzed to determine its potential food application. The results revealed that the studied edible insect production system has beneficial environmental effects on various impact categories (ICs), i.e., land occupation, mineral extraction, aquatic and terrestrial ecotoxicity, due to utilization of bio-waste to feed insects. This food production system can mitigate the negative environmental effects of those ICs, but has negative environmental impact on some other ICs such as global warming potential. By managing the consumption of various inputs, edible insects can become an environmentally efficient food production system for human nutrition.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source
VL  - 11
IS  - 1
SP  - 14030
DO  - 10.1038/s41598-021-93284-8
ER  - 

@article{
author = "Nikkhah, Amin and Van Haute, Sam and Jovanović, Vesna B. and Jung, Heejung and Dewulf, Jo and Ćirković-Veličković, Tanja and Ghnimi, Sami",
year = "2021",
abstract = "Because it is important to develop new sustainable sources of edible protein, insects have been recommended as a new protein source. This study applied Life Cycle Assessment (LCA) to investigate the environmental impact of small-scale edible insect production unit in South Korea. IMPACT 2002 + was applied as the baseline impact assessment (IA) methodology. The CML-IA baseline, EDIP 2003, EDP 2013, ILCD 2011 Midpoint, and ReCiPe midpoint IA methodologies were also used for LCIA methodology sensitivity analysis. The protein, fat contents, and fatty acid profile of the investigated insect (Protaetia brevitarsis seulensis larvae) were analyzed to determine its potential food application. The results revealed that the studied edible insect production system has beneficial environmental effects on various impact categories (ICs), i.e., land occupation, mineral extraction, aquatic and terrestrial ecotoxicity, due to utilization of bio-waste to feed insects. This food production system can mitigate the negative environmental effects of those ICs, but has negative environmental impact on some other ICs such as global warming potential. By managing the consumption of various inputs, edible insects can become an environmentally efficient food production system for human nutrition.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source",
volume = "11",
number = "1",
pages = "14030",
doi = "10.1038/s41598-021-93284-8"
}

Nikkhah, A., Van Haute, S., Jovanović, V. B., Jung, H., Dewulf, J., Ćirković-Veličković, T.,& Ghnimi, S.. (2021). Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source. in Scientific Reports
Springer Nature., 11(1), 14030.
https://doi.org/10.1038/s41598-021-93284-8

Nikkhah A, Van Haute S, Jovanović VB, Jung H, Dewulf J, Ćirković-Veličković T, Ghnimi S. Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source. in Scientific Reports. 2021;11(1):14030.
doi:10.1038/s41598-021-93284-8 .

Nikkhah, Amin, Van Haute, Sam, Jovanović, Vesna B., Jung, Heejung, Dewulf, Jo, Ćirković-Veličković, Tanja, Ghnimi, Sami, "Life cycle assessment of edible insects (Protaetia brevitarsis seulensis larvae) as a future protein and fat source" in Scientific Reports, 11, no. 1 (2021):14030,
https://doi.org/10.1038/s41598-021-93284-8 . .

TY  - JOUR
AU  - Nikkhah, Amin
AU  - Van Haute, Sam
AU  - Jovanović, Vesna B.
AU  - Jung, Heejung
AU  - Dewulf, Jo
AU  - Ćirković-Veličković, Tanja
AU  - Ghnimi, Sami
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4586
AB  - In the original version of this Article, Sam Van Haute and Sami Ghnimi were omitted as corresponding authors.
Correspondence and requests for materials should also be addressed to sam.vanhaute@ghent.ac.kr and sghnimi@
isara.fr.
The original Article has been corrected.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Correction to:  Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. DOI: https://doi.org/10.1038/s41598-021-93284-8)
VL  - 11
IS  - 1
SP  - 14030
DO  - 10.1038/s41598-021-97513-y
ER  - 

@article{
author = "Nikkhah, Amin and Van Haute, Sam and Jovanović, Vesna B. and Jung, Heejung and Dewulf, Jo and Ćirković-Veličković, Tanja and Ghnimi, Sami",
year = "2021",
abstract = "In the original version of this Article, Sam Van Haute and Sami Ghnimi were omitted as corresponding authors.
Correspondence and requests for materials should also be addressed to sam.vanhaute@ghent.ac.kr and sghnimi@
isara.fr.
The original Article has been corrected.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Correction to:  Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. DOI: https://doi.org/10.1038/s41598-021-93284-8)",
volume = "11",
number = "1",
pages = "14030",
doi = "10.1038/s41598-021-97513-y"
}

Nikkhah, A., Van Haute, S., Jovanović, V. B., Jung, H., Dewulf, J., Ćirković-Veličković, T.,& Ghnimi, S.. (2021). Correction to:  Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. DOI: https://doi.org/10.1038/s41598-021-93284-8). in Scientific Reports
Springer Nature., 11(1), 14030.
https://doi.org/10.1038/s41598-021-97513-y

Nikkhah A, Van Haute S, Jovanović VB, Jung H, Dewulf J, Ćirković-Veličković T, Ghnimi S. Correction to:  Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. DOI: https://doi.org/10.1038/s41598-021-93284-8). in Scientific Reports. 2021;11(1):14030.
doi:10.1038/s41598-021-97513-y .

Nikkhah, Amin, Van Haute, Sam, Jovanović, Vesna B., Jung, Heejung, Dewulf, Jo, Ćirković-Veličković, Tanja, Ghnimi, Sami, "Correction to:  Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. DOI: https://doi.org/10.1038/s41598-021-93284-8)" in Scientific Reports, 11, no. 1 (2021):14030,
https://doi.org/10.1038/s41598-021-97513-y . .

TY  - DATA
AU  - Nikkhah, Amin
AU  - Van Haute, Sam
AU  - Jovanović, Vesna B.
AU  - Jung, Heejung
AU  - Dewulf, Jo
AU  - Ćirković-Veličković, Tanja
AU  - Ghnimi, Sami
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4587
PB  - Springer Nature
T2  - Scientific Reports
T1  - Supplementary data for the article: Nikkhah, A.; Van Haute, S.; Jovanović, V.; Jung, H.; Dewulf, J.; Ćirković-Veličković, T.; Ghnimi, S. Correction to: Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. https://doi.org/10.1038/S41598-021-93284-8).
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4587
ER  - 

@misc{
author = "Nikkhah, Amin and Van Haute, Sam and Jovanović, Vesna B. and Jung, Heejung and Dewulf, Jo and Ćirković-Veličković, Tanja and Ghnimi, Sami",
year = "2021",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Supplementary data for the article: Nikkhah, A.; Van Haute, S.; Jovanović, V.; Jung, H.; Dewulf, J.; Ćirković-Veličković, T.; Ghnimi, S. Correction to: Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. https://doi.org/10.1038/S41598-021-93284-8).",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4587"
}

Nikkhah, A., Van Haute, S., Jovanović, V. B., Jung, H., Dewulf, J., Ćirković-Veličković, T.,& Ghnimi, S.. (2021). Supplementary data for the article: Nikkhah, A.; Van Haute, S.; Jovanović, V.; Jung, H.; Dewulf, J.; Ćirković-Veličković, T.; Ghnimi, S. Correction to: Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. https://doi.org/10.1038/S41598-021-93284-8).. in Scientific Reports
Springer Nature..
https://hdl.handle.net/21.15107/rcub_cherry_4587

Nikkhah A, Van Haute S, Jovanović VB, Jung H, Dewulf J, Ćirković-Veličković T, Ghnimi S. Supplementary data for the article: Nikkhah, A.; Van Haute, S.; Jovanović, V.; Jung, H.; Dewulf, J.; Ćirković-Veličković, T.; Ghnimi, S. Correction to: Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. https://doi.org/10.1038/S41598-021-93284-8).. in Scientific Reports. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4587 .

Nikkhah, Amin, Van Haute, Sam, Jovanović, Vesna B., Jung, Heejung, Dewulf, Jo, Ćirković-Veličković, Tanja, Ghnimi, Sami, "Supplementary data for the article: Nikkhah, A.; Van Haute, S.; Jovanović, V.; Jung, H.; Dewulf, J.; Ćirković-Veličković, T.; Ghnimi, S. Correction to: Life Cycle Assessment of Edible Insects (Protaetia Brevitarsis Seulensis Larvae) as a Future Protein and Fat Source (Sci Rep 2021, 11 (1), 14030. https://doi.org/10.1038/S41598-021-93284-8)." in Scientific Reports (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4587 .

TY  - CONF
AU  - Mladenović, Maja
AU  - Apostolović, Danijela
AU  - Smiljanić, Katarina
AU  - Jovanović, Vesna B.
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5734
AB  - Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Our aim was to probe for the presence potentially novel allergens in two blood cockles, containing hemoglobin, Anadara broughtonii (AB) and Tigellarca granosa (TG), using sera of patients allergic to seashells and shrimps. Seashells estracts were resolved by 1D and 2D-SDS-PAGE, which were in-gel trypsin digested and characterized with nLC-ESI-MS/MS. The presence of tropomyosin was confirmed by commercial tropomyosin standard, by 1D/2D blots with specific antibodies, and by mass spectrometry identification. 1D-immunoblot was used for individual patients' profiling of IgE binding, while 2D-immunoblot was used to asses IgE reactivity with the pool of patients' sera. One third of 69sera of tested patients allergic to seafood or shrimps showed cross-reactivity with blood clamps. 2D-electrophoresis showed that most of proteins are in acidic range with especially in the range 35-50 kDa, with tropomyosin's isoforms presence in majority of spots in both seashells. IgE reactivity of individual patients showed great pattern diversity in 1D-immunoblot. 2D-immunoblots suggests hemoglobin and arginine kinase could be novel allergens. In-depth characterization of proteins causing IgE-mediated allergies is important because it can improve diagnosis and patients' health management.
C3  - Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71
T1  - The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin
SP  - 59
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5734
ER  - 

@conference{
author = "Mladenović, Maja and Apostolović, Danijela and Smiljanić, Katarina and Jovanović, Vesna B. and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "Shellfish allergy is one of the most common food allergies with a prevalence of 0.5%-2.5% in the general population. The most common allergen present in shellfish is tropomyosin. Our aim was to probe for the presence potentially novel allergens in two blood cockles, containing hemoglobin, Anadara broughtonii (AB) and Tigellarca granosa (TG), using sera of patients allergic to seashells and shrimps. Seashells estracts were resolved by 1D and 2D-SDS-PAGE, which were in-gel trypsin digested and characterized with nLC-ESI-MS/MS. The presence of tropomyosin was confirmed by commercial tropomyosin standard, by 1D/2D blots with specific antibodies, and by mass spectrometry identification. 1D-immunoblot was used for individual patients' profiling of IgE binding, while 2D-immunoblot was used to asses IgE reactivity with the pool of patients' sera. One third of 69sera of tested patients allergic to seafood or shrimps showed cross-reactivity with blood clamps. 2D-electrophoresis showed that most of proteins are in acidic range with especially in the range 35-50 kDa, with tropomyosin's isoforms presence in majority of spots in both seashells. IgE reactivity of individual patients showed great pattern diversity in 1D-immunoblot. 2D-immunoblots suggests hemoglobin and arginine kinase could be novel allergens. In-depth characterization of proteins causing IgE-mediated allergies is important because it can improve diagnosis and patients' health management.",
journal = "Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71",
title = "The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin",
pages = "59-59",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5734"
}

Mladenović, M., Apostolović, D., Smiljanić, K., Jovanović, V. B.,& Ćirković-Veličković, T.. (2021). The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71, 59-59.
https://hdl.handle.net/21.15107/rcub_cherry_5734

Mladenović M, Apostolović D, Smiljanić K, Jovanović VB, Ćirković-Veličković T. The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin. in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71. 2021;:59-59.
https://hdl.handle.net/21.15107/rcub_cherry_5734 .

Mladenović, Maja, Apostolović, Danijela, Smiljanić, Katarina, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "The immunoproteomics reveals novel potential allergens in ark clam seashells, besides abundant tropomyosin" in Proteomics and Metabolomics for Personalized Medicine, XV Italian Proteomics Association Annual Meeting, Catholic University of the Sacred Heart, Roma, Italy, 8th-10th September 2021, 2021, 71-71 (2021):59-59,
https://hdl.handle.net/21.15107/rcub_cherry_5734 .

TY  - CHAP
AU  - Smiljanić, Katarina
AU  - Mihailović, Jelena
AU  - Prodić, Ivana
AU  - Đukić, Teodora
AU  - Vasović, Tamara
AU  - Jovanović, Vesna B.
AU  - Ćirković-Veličković, Tanja
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5728
AB  - Post-translational modifications (PTMs) occur in many forms and shapes, widely influencing protein behavior. High-resolution tandem mass spectrometry (HRMS/MS), coupled with dedicated engines for the identification of unspecified PTMs, is a powerful method for their mapping. 
A majority of proteomic experiments utilize trypsin for digestion, which cleaves the C-terminal peptide bonds of arginine (Arg) and lysine (Lys) amino acids with high catalytic efficiency and selectivity, unless they are followed with proline. At the same time, Arg and Lys residues are frequently modified during food processing by heat and non-thermal treatments, causing oxidation, carbamylation, and various forms of side chain carbonylation, including the other common PTMs (methylation, acetylation, etc.). Consequently, we explored the possibility to re-assess already generated proteomic data (food protein/allergen tryptic peptides) with respect to the possible modulation of the tryptic intestinal digestion pattern caused by PTMs incorporated at Arg and Lys residues. However, most of the proteomic bottom-up experiments are run with porcine trypsin that has been reductively methylated to increase its stability and minimize autoproteolytic effects. Therefore, in this chapter, the utility of the aforementioned idea was explored, by reviewing the differences in structure, affinity, specificity, and catalytic efficiency of trypsin, primarily from porcine, bovine and human species. Porcine trypsin either from pancreas or in recombinant form showed superior performance compared to human and bovine tryptic counterparts. In addition, set of software tools for identification and analyses of PTMs was reviewed with the aim to isolate those capable of in-depth PTMs profiling and their simultaneous relative quantification, such as PEAKS PTM (PEAKS Studio, Bioinformatics Solution Inc., Ontario Canada). Based on our preliminary experimental results, conclusion is that the proposed idea is plausible, because if potential hindrance effects caused by PTMs are observed with porcine trypsin, then they can be just augmented within human intestinal digestion, with respect to inferior performance of human trypsin.
PB  - New York : Nova Science Publisher
T2  - A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era
T1  - Trypsin as a Proteomic Probe for Assessment of Food Protein Digestibility in Relation to Chemical and Post-translational Modifications
VL  - 4
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5728
ER  - 

@inbook{
author = "Smiljanić, Katarina and Mihailović, Jelena and Prodić, Ivana and Đukić, Teodora and Vasović, Tamara and Jovanović, Vesna B. and Ćirković-Veličković, Tanja",
year = "2020",
abstract = "Post-translational modifications (PTMs) occur in many forms and shapes, widely influencing protein behavior. High-resolution tandem mass spectrometry (HRMS/MS), coupled with dedicated engines for the identification of unspecified PTMs, is a powerful method for their mapping. 
A majority of proteomic experiments utilize trypsin for digestion, which cleaves the C-terminal peptide bonds of arginine (Arg) and lysine (Lys) amino acids with high catalytic efficiency and selectivity, unless they are followed with proline. At the same time, Arg and Lys residues are frequently modified during food processing by heat and non-thermal treatments, causing oxidation, carbamylation, and various forms of side chain carbonylation, including the other common PTMs (methylation, acetylation, etc.). Consequently, we explored the possibility to re-assess already generated proteomic data (food protein/allergen tryptic peptides) with respect to the possible modulation of the tryptic intestinal digestion pattern caused by PTMs incorporated at Arg and Lys residues. However, most of the proteomic bottom-up experiments are run with porcine trypsin that has been reductively methylated to increase its stability and minimize autoproteolytic effects. Therefore, in this chapter, the utility of the aforementioned idea was explored, by reviewing the differences in structure, affinity, specificity, and catalytic efficiency of trypsin, primarily from porcine, bovine and human species. Porcine trypsin either from pancreas or in recombinant form showed superior performance compared to human and bovine tryptic counterparts. In addition, set of software tools for identification and analyses of PTMs was reviewed with the aim to isolate those capable of in-depth PTMs profiling and their simultaneous relative quantification, such as PEAKS PTM (PEAKS Studio, Bioinformatics Solution Inc., Ontario Canada). Based on our preliminary experimental results, conclusion is that the proposed idea is plausible, because if potential hindrance effects caused by PTMs are observed with porcine trypsin, then they can be just augmented within human intestinal digestion, with respect to inferior performance of human trypsin.",
publisher = "New York : Nova Science Publisher",
journal = "A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era",
booktitle = "Trypsin as a Proteomic Probe for Assessment of Food Protein Digestibility in Relation to Chemical and Post-translational Modifications",
volume = "4",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5728"
}

Smiljanić, K., Mihailović, J., Prodić, I., Đukić, T., Vasović, T., Jovanović, V. B.,& Ćirković-Veličković, T.. (2020). Trypsin as a Proteomic Probe for Assessment of Food Protein Digestibility in Relation to Chemical and Post-translational Modifications. in A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era
New York : Nova Science Publisher., 4, 158.
https://hdl.handle.net/21.15107/rcub_cherry_5728

Smiljanić K, Mihailović J, Prodić I, Đukić T, Vasović T, Jovanović VB, Ćirković-Veličković T. Trypsin as a Proteomic Probe for Assessment of Food Protein Digestibility in Relation to Chemical and Post-translational Modifications. in A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era. 2020;4:158.
https://hdl.handle.net/21.15107/rcub_cherry_5728 .

Smiljanić, Katarina, Mihailović, Jelena, Prodić, Ivana, Đukić, Teodora, Vasović, Tamara, Jovanović, Vesna B., Ćirković-Veličković, Tanja, "Trypsin as a Proteomic Probe for Assessment of Food Protein Digestibility in Relation to Chemical and Post-translational Modifications" in A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era, 4 (2020):158,
https://hdl.handle.net/21.15107/rcub_cherry_5728 .

TY  - JOUR
AU  - Filipović, Nenad R.
AU  - Bjelogrlić, Snežana K.
AU  - Pelliccia, Sveva
AU  - Jovanović, Vesna B.
AU  - Kojić, Milan O.
AU  - Senćanski, Milan
AU  - La Regina, Giuseppe
AU  - Silvestri, Romano
AU  - Muller, Christian D.
AU  - Todorović, Tamara
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/320
AB  - Triapine, the most studied α-N-heterocyclic thiosemicarbazone, revealed potent activity against advanced leukemia, but was ineffective against a variety of solid tumors. Moreover, methemoglobinemia, which is a side effect of triapine administration, may limits all clinical application. To enhance anticancer activity and reduce side effects, we applied an isosteric replacement of sulfur to selenium atom was performed by synthesis and characterization of selenium triapine analog, 3-aminopyridine-2-carboxaldehyde selenosemicarbazone (selenotriapine). Compared to triapine, selenotriapine revealed superior pro-apoptotic activity with activation of intrinsic apoptotic pathway in both human monocytic leukemia (THP-1) and mammary adenocarcinoma (MCF-7) cell lines. For MCF-7 2-D cultures, selenotriapine induced notable increase in mitochondrial superoxide radical generation and dissipation of mitochondrial transmembrane potential. A significant delay in growth of MCF-7 spheroids (3-D culture) was accompanied by phenotypic stem cell reprogramming (Oct-4 expression). Additionally, selenotriapine demonstrated a very low toxicity profile as compared to triapine, confirmed over alleviated extent of methemoglobin formation and higher IC50 value in brine shrimp cytotoxicity assay. © 2017 King Saud University.
T2  - Arabian Journal of Chemistry
T1  - Selenotriapine - An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors
DO  - 10.1016/j.arabjc.2017.11.017
ER  - 

@article{
author = "Filipović, Nenad R. and Bjelogrlić, Snežana K. and Pelliccia, Sveva and Jovanović, Vesna B. and Kojić, Milan O. and Senćanski, Milan and La Regina, Giuseppe and Silvestri, Romano and Muller, Christian D. and Todorović, Tamara",
year = "2020",
abstract = "Triapine, the most studied α-N-heterocyclic thiosemicarbazone, revealed potent activity against advanced leukemia, but was ineffective against a variety of solid tumors. Moreover, methemoglobinemia, which is a side effect of triapine administration, may limits all clinical application. To enhance anticancer activity and reduce side effects, we applied an isosteric replacement of sulfur to selenium atom was performed by synthesis and characterization of selenium triapine analog, 3-aminopyridine-2-carboxaldehyde selenosemicarbazone (selenotriapine). Compared to triapine, selenotriapine revealed superior pro-apoptotic activity with activation of intrinsic apoptotic pathway in both human monocytic leukemia (THP-1) and mammary adenocarcinoma (MCF-7) cell lines. For MCF-7 2-D cultures, selenotriapine induced notable increase in mitochondrial superoxide radical generation and dissipation of mitochondrial transmembrane potential. A significant delay in growth of MCF-7 spheroids (3-D culture) was accompanied by phenotypic stem cell reprogramming (Oct-4 expression). Additionally, selenotriapine demonstrated a very low toxicity profile as compared to triapine, confirmed over alleviated extent of methemoglobin formation and higher IC50 value in brine shrimp cytotoxicity assay. © 2017 King Saud University.",
journal = "Arabian Journal of Chemistry",
title = "Selenotriapine - An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors",
doi = "10.1016/j.arabjc.2017.11.017"
}

Filipović, N. R., Bjelogrlić, S. K., Pelliccia, S., Jovanović, V. B., Kojić, M. O., Senćanski, M., La Regina, G., Silvestri, R., Muller, C. D.,& Todorović, T.. (2020). Selenotriapine - An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors. in Arabian Journal of Chemistry.
https://doi.org/10.1016/j.arabjc.2017.11.017

Filipović NR, Bjelogrlić SK, Pelliccia S, Jovanović VB, Kojić MO, Senćanski M, La Regina G, Silvestri R, Muller CD, Todorović T. Selenotriapine - An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors. in Arabian Journal of Chemistry. 2020;.
doi:10.1016/j.arabjc.2017.11.017 .

Filipović, Nenad R., Bjelogrlić, Snežana K., Pelliccia, Sveva, Jovanović, Vesna B., Kojić, Milan O., Senćanski, Milan, La Regina, Giuseppe, Silvestri, Romano, Muller, Christian D., Todorović, Tamara, "Selenotriapine - An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors" in Arabian Journal of Chemistry (2020),
https://doi.org/10.1016/j.arabjc.2017.11.017 . .

TY  - JOUR
AU  - Krstić-Ristivojević, Maja
AU  - Grundström, Jeanette
AU  - Apostolović, Danijela
AU  - Radomirović, Mirjana Ž.
AU  - Jovanović, Vesna B.
AU  - Radoi, Vlad
AU  - Kiewiet, M. B. Gea
AU  - Vukojević, Vladana
AU  - Ćirković-Veličković, Tanja
AU  - van Hage, Marianne
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4866
AB  - Transepithelial transport of proteins is an important step in the immune response to food allergens. Mammalian meat allergy is characterized by an IgE response against the carbohydrate moiety galactosyl-α-1,3-galactose (α-Gal) present on mammalian glycoproteins and glycolipids, which causes severe allergic reactions several hours after red meat consumption. The delayed reaction may be related to the processing of α-Gal carrying proteins in the gastrointestinal tract. The aim of this study was to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer. We found that α-Gal glycosylation altered protein susceptibility to gastric digestion, where large protein fragments bearing the α-Gal epitope remained for up to 2 h of digestion. Furthermore, α-Gal glycosylation of the protein hampered transcytosis of the protein through the Caco-2 monolayer. α-Gal epitope on the intact protein could be detected in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates. Furthermore, the level of galectin-3 in Caco-2 cells was not affected by the presence of α-Gal glycosylated BSA (bovine serum albumin) (BSA-α-Gal). Taken together, our data add new knowledge and shed light on the digestion and transport of α-Gal glycosylated proteins.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer
VL  - 21
IS  - 16
SP  - 5742
DO  - 10.3390/ijms21165742
ER  - 

@article{
author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Apostolović, Danijela and Radomirović, Mirjana Ž. and Jovanović, Vesna B. and Radoi, Vlad and Kiewiet, M. B. Gea and Vukojević, Vladana and Ćirković-Veličković, Tanja and van Hage, Marianne",
year = "2020",
abstract = "Transepithelial transport of proteins is an important step in the immune response to food allergens. Mammalian meat allergy is characterized by an IgE response against the carbohydrate moiety galactosyl-α-1,3-galactose (α-Gal) present on mammalian glycoproteins and glycolipids, which causes severe allergic reactions several hours after red meat consumption. The delayed reaction may be related to the processing of α-Gal carrying proteins in the gastrointestinal tract. The aim of this study was to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer. We found that α-Gal glycosylation altered protein susceptibility to gastric digestion, where large protein fragments bearing the α-Gal epitope remained for up to 2 h of digestion. Furthermore, α-Gal glycosylation of the protein hampered transcytosis of the protein through the Caco-2 monolayer. α-Gal epitope on the intact protein could be detected in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates. Furthermore, the level of galectin-3 in Caco-2 cells was not affected by the presence of α-Gal glycosylated BSA (bovine serum albumin) (BSA-α-Gal). Taken together, our data add new knowledge and shed light on the digestion and transport of α-Gal glycosylated proteins.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer",
volume = "21",
number = "16",
pages = "5742",
doi = "10.3390/ijms21165742"
}

Krstić-Ristivojević, M., Grundström, J., Apostolović, D., Radomirović, M. Ž., Jovanović, V. B., Radoi, V., Kiewiet, M. B. G., Vukojević, V., Ćirković-Veličković, T.,& van Hage, M.. (2020). Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer. in International Journal of Molecular Sciences
MDPI., 21(16), 5742.
https://doi.org/10.3390/ijms21165742

Krstić-Ristivojević M, Grundström J, Apostolović D, Radomirović MŽ, Jovanović VB, Radoi V, Kiewiet MBG, Vukojević V, Ćirković-Veličković T, van Hage M. Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer. in International Journal of Molecular Sciences. 2020;21(16):5742.
doi:10.3390/ijms21165742 .

Krstić-Ristivojević, Maja, Grundström, Jeanette, Apostolović, Danijela, Radomirović, Mirjana Ž., Jovanović, Vesna B., Radoi, Vlad, Kiewiet, M. B. Gea, Vukojević, Vladana, Ćirković-Veličković, Tanja, van Hage, Marianne, "Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer" in International Journal of Molecular Sciences, 21, no. 16 (2020):5742,
https://doi.org/10.3390/ijms21165742 . .

TY  - CHAP
AU  - Jovanović, Vesna B.
AU  - Smiljanić, Katarina
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5729
AB  - In the human body there are at least 500-600 different proteases, the enzymes capable to catalyze hydrolysis of peptide bond in the proteins. The process of proteolysis is responsible for many physiological processes such as amino acid recycling, removing of aged, misfolded or damaged proteins, development and differentiation, antigen presentation, apoptosis, necrosis, inflammation, cell signaling, survival, and proliferation, wound healing, and immunity. This chapter will provide an overview of the main classes of proteases and the common criteria used for their classification (type of reaction catalyzed, chemical nature of the catalytic site (metalloproteases, aspartic, glutamic, threonine, serine, and cysteine proteases)). In addition to that, evolutionary relationship with reference to diversity of protease structures (MEROPS and Degradome databases), as well as mechanisms of proteolysis reaction, and of regulation of proteases activity, including their substrates and inhibitors, will be enclosed. Further, chapter progresses into proteases distribution pattern within the human’s tissues in healthy condition. The special attention will be dedicate to cathepsins, caspases, calpains and matrix metalloproteases, the most studied four classes of proteases in different physiological and pathological conditions. Their classification, localization in cell, expression pattern, substrates and mechanisms of regulation of activity will be given in respect to obesity, diabetes, insulin resistance, and the most common four types of cancer (gastric, ovarian, breast, and lung). Also, the brief overview of proteases, proposed as the potential diagnostic, prognostic markers and therapeutic targets, will be elaborated. The chapter encompasses with advantages and disadvantages of endogenous and exogenous protease inhibitors application in the potential treatment of like cardiovascular, neurodegenerative, cancer diseases.
PB  - New York : Nova Science Publisher
T2  - A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era
T1  - Proteases: Our Friends or Foes
VL  - 1
SP  - 1
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5729
ER  - 

@inbook{
author = "Jovanović, Vesna B. and Smiljanić, Katarina",
year = "2020",
abstract = "In the human body there are at least 500-600 different proteases, the enzymes capable to catalyze hydrolysis of peptide bond in the proteins. The process of proteolysis is responsible for many physiological processes such as amino acid recycling, removing of aged, misfolded or damaged proteins, development and differentiation, antigen presentation, apoptosis, necrosis, inflammation, cell signaling, survival, and proliferation, wound healing, and immunity. This chapter will provide an overview of the main classes of proteases and the common criteria used for their classification (type of reaction catalyzed, chemical nature of the catalytic site (metalloproteases, aspartic, glutamic, threonine, serine, and cysteine proteases)). In addition to that, evolutionary relationship with reference to diversity of protease structures (MEROPS and Degradome databases), as well as mechanisms of proteolysis reaction, and of regulation of proteases activity, including their substrates and inhibitors, will be enclosed. Further, chapter progresses into proteases distribution pattern within the human’s tissues in healthy condition. The special attention will be dedicate to cathepsins, caspases, calpains and matrix metalloproteases, the most studied four classes of proteases in different physiological and pathological conditions. Their classification, localization in cell, expression pattern, substrates and mechanisms of regulation of activity will be given in respect to obesity, diabetes, insulin resistance, and the most common four types of cancer (gastric, ovarian, breast, and lung). Also, the brief overview of proteases, proposed as the potential diagnostic, prognostic markers and therapeutic targets, will be elaborated. The chapter encompasses with advantages and disadvantages of endogenous and exogenous protease inhibitors application in the potential treatment of like cardiovascular, neurodegenerative, cancer diseases.",
publisher = "New York : Nova Science Publisher",
journal = "A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era",
booktitle = "Proteases: Our Friends or Foes",
volume = "1",
pages = "1-28",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5729"
}

Jovanović, V. B.,& Smiljanić, K.. (2020). Proteases: Our Friends or Foes. in A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era
New York : Nova Science Publisher., 1, 1-28.
https://hdl.handle.net/21.15107/rcub_cherry_5729

Jovanović VB, Smiljanić K. Proteases: Our Friends or Foes. in A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era. 2020;1:1-28.
https://hdl.handle.net/21.15107/rcub_cherry_5729 .

Jovanović, Vesna B., Smiljanić, Katarina, "Proteases: Our Friends or Foes" in A Closer Look at Proteolysis: Biochemistry and Molecular Biology in the Post Genomic Era, 1 (2020):1-28,
https://hdl.handle.net/21.15107/rcub_cherry_5729 .

TY  - CONF
AU  - Mutić, Jelena
AU  - Jovanović, Vesna B.
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
AU  - Đurđić, Slađana Z.
AU  - Ćirković-Veličković, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3760
AB  - Cilj ove studije je klasifikacija biološki različitih vrsta školjki na osnovu sadržaja esencijalnih elemenata primenom hemometrije. Sadržaj esencijalnih elemenata kao što su Co, Cr, Cu, Mn, Ni, Se, Zn, i Fe je određen u četiri biološki različite vrste školjki Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) i Anadara brоughtonii (AB). Analizitani uzorci su kupljeni u Incheonu, Koreja. Sadržaj esenicjalnih elemenata je određen primenom induktivno spregnute plazme kuplovane sa masenom spektrometrijom (ICP-MS) nakon mikrotalasne digestije. Hemometrijske tehnike pokazuju grupisanje ispitivanih uzoraka školjki prema sadržaju esencijalnih elemenata i identifikuju elemente najvažnije za klasifikaciju.
AB  - The main aim of current study was classification of four biologically different sellfish species such as bivalve molluscs Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) and Anadara broughtonii (AB) bought in the Incheon, South Korea. Content of essencial ellements such as Co, Cr, Cu, Mn, Ni, Se, Zn, and Fe were determined by using inductively coupled plasma mass spectrometry (ICP-MS) after closed-vessel microwave digestion. Chemometrics techniques showed classification of sellfish smaples based on biological species and identified elements most important for classification.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.
T1  - Klasifikacija školjki na osnovu sadržaja esencijalnih elemenata i hemometrije
T1  - Chemometric characterization of sellfish according to their element composition
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3760
ER  - 

@conference{
author = "Mutić, Jelena and Jovanović, Vesna B. and Ristivojević, Petar and Milojković-Opsenica, Dušanka and Đurđić, Slađana Z. and Ćirković-Veličković, Tanja",
year = "2019",
abstract = "Cilj ove studije je klasifikacija biološki različitih vrsta školjki na osnovu sadržaja esencijalnih elemenata primenom hemometrije. Sadržaj esencijalnih elemenata kao što su Co, Cr, Cu, Mn, Ni, Se, Zn, i Fe je određen u četiri biološki različite vrste školjki Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) i Anadara brоughtonii (AB). Analizitani uzorci su kupljeni u Incheonu, Koreja. Sadržaj esenicjalnih elemenata je određen primenom induktivno spregnute plazme kuplovane sa masenom spektrometrijom (ICP-MS) nakon mikrotalasne digestije. Hemometrijske tehnike pokazuju grupisanje ispitivanih uzoraka školjki prema sadržaju esencijalnih elemenata i identifikuju elemente najvažnije za klasifikaciju., The main aim of current study was classification of four biologically different sellfish species such as bivalve molluscs Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) and Anadara broughtonii (AB) bought in the Incheon, South Korea. Content of essencial ellements such as Co, Cr, Cu, Mn, Ni, Se, Zn, and Fe were determined by using inductively coupled plasma mass spectrometry (ICP-MS) after closed-vessel microwave digestion. Chemometrics techniques showed classification of sellfish smaples based on biological species and identified elements most important for classification.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.",
title = "Klasifikacija školjki na osnovu sadržaja esencijalnih elemenata i hemometrije, Chemometric characterization of sellfish according to their element composition",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3760"
}

Mutić, J., Jovanović, V. B., Ristivojević, P., Milojković-Opsenica, D., Đurđić, S. Z.,& Ćirković-Veličković, T.. (2019). Klasifikacija školjki na osnovu sadržaja esencijalnih elemenata i hemometrije. in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_cherry_3760

Mutić J, Jovanović VB, Ristivojević P, Milojković-Opsenica D, Đurđić SZ, Ćirković-Veličković T. Klasifikacija školjki na osnovu sadržaja esencijalnih elemenata i hemometrije. in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3760 .

Mutić, Jelena, Jovanović, Vesna B., Ristivojević, Petar, Milojković-Opsenica, Dušanka, Đurđić, Slađana Z., Ćirković-Veličković, Tanja, "Klasifikacija školjki na osnovu sadržaja esencijalnih elemenata i hemometrije" in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019. (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3760 .

TY  - CONF
AU  - Đurđić, Slađana Z.
AU  - Jovanović, Vesna B.
AU  - Tomić, Sofija
AU  - Ćirković-Veličković, Tanja
AU  - Mutić, Jelena
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3762
AB  - Morski plodovi imaju visoku nutritivnu vrednost, ali sa druge strane postoji rizik od kontaminacije toksičnim elementima. Sadržaj As, Cd, Hg i Pb je određen u četiri vrste školjki Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) i Anadara broughtonii (AB) kupljene u Incheonu, Koreja. Uzorci su analizirani induktivno spregnutom plazmom - masenom spektrometrijom (ICP-MS) nakon mikrotalasne digestije. Izračunati su dnevni/nedeljni unosi za ove elemente u mg/300 g uzorka. Ukupan sadržaj As (neoganski i organski) u svim vrstama je bio veći od dozvoljenog limita preporučenog od Svetske zdravstvene organizacije (WHO). Procenjeni dnevni unosi ostalih elemenata (Hg, Cd i Pb) su niži od maksimalnih podnošljivih granica (MDI) koje je odredila Evropska agencija za bezbednost hrane (EFSA) što ukazuje da nema rizika za konzumente.
AB  - Bivalve molluscs, which include mussels, oysters and clams, have high nutritional value. On the other hand, seafood may also contain harmful contaminants and other undesirable substances such as mercury and persistent halogenated compounds. Four species of bivalve molluscs Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) and Anadara broughtonii (AB) were bought in Incheon, Korea, in order to determine content of As, Cd, Hg, and Pb and consequently, to estimate the health hazards associated to dietary intake. The samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion. All species showed As content higher than the maximum tolerable limit specified by EFSA. Estimated daily intake of Hg, Cd and Pb from consumption of 300 g was very low and hence poses no toxicological risk.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.
T1  - Određivanje toksičnih elemenata (žive, kadmijuma, olova i arsena) u uzorcima školjki
T1  - Determination of toxic elements (mercury, cadmium, lead and arsenic) in shellfish samples
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3762
ER  - 

@conference{
author = "Đurđić, Slađana Z. and Jovanović, Vesna B. and Tomić, Sofija and Ćirković-Veličković, Tanja and Mutić, Jelena",
year = "2019",
abstract = "Morski plodovi imaju visoku nutritivnu vrednost, ali sa druge strane postoji rizik od kontaminacije toksičnim elementima. Sadržaj As, Cd, Hg i Pb je određen u četiri vrste školjki Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) i Anadara broughtonii (AB) kupljene u Incheonu, Koreja. Uzorci su analizirani induktivno spregnutom plazmom - masenom spektrometrijom (ICP-MS) nakon mikrotalasne digestije. Izračunati su dnevni/nedeljni unosi za ove elemente u mg/300 g uzorka. Ukupan sadržaj As (neoganski i organski) u svim vrstama je bio veći od dozvoljenog limita preporučenog od Svetske zdravstvene organizacije (WHO). Procenjeni dnevni unosi ostalih elemenata (Hg, Cd i Pb) su niži od maksimalnih podnošljivih granica (MDI) koje je odredila Evropska agencija za bezbednost hrane (EFSA) što ukazuje da nema rizika za konzumente., Bivalve molluscs, which include mussels, oysters and clams, have high nutritional value. On the other hand, seafood may also contain harmful contaminants and other undesirable substances such as mercury and persistent halogenated compounds. Four species of bivalve molluscs Ruditapes philippinarum (Manila clam, MC), Yesso scallop (YS), Tegillarca granosa (TG) and Anadara broughtonii (AB) were bought in Incheon, Korea, in order to determine content of As, Cd, Hg, and Pb and consequently, to estimate the health hazards associated to dietary intake. The samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion. All species showed As content higher than the maximum tolerable limit specified by EFSA. Estimated daily intake of Hg, Cd and Pb from consumption of 300 g was very low and hence poses no toxicological risk.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.",
title = "Određivanje toksičnih elemenata (žive, kadmijuma, olova i arsena) u uzorcima školjki, Determination of toxic elements (mercury, cadmium, lead and arsenic) in shellfish samples",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3762"
}

Đurđić, S. Z., Jovanović, V. B., Tomić, S., Ćirković-Veličković, T.,& Mutić, J.. (2019). Određivanje toksičnih elemenata (žive, kadmijuma, olova i arsena) u uzorcima školjki. in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_cherry_3762

Đurđić SZ, Jovanović VB, Tomić S, Ćirković-Veličković T, Mutić J. Određivanje toksičnih elemenata (žive, kadmijuma, olova i arsena) u uzorcima školjki. in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019.. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3762 .

Đurđić, Slađana Z., Jovanović, Vesna B., Tomić, Sofija, Ćirković-Veličković, Tanja, Mutić, Jelena, "Određivanje toksičnih elemenata (žive, kadmijuma, olova i arsena) u uzorcima školjki" in Kratki izvodi radova / 56. savetovanje Srpskog hemijskog društva , Niš 7. i 8. juni 2019. (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3762 .