TY  - CONF
AU  - Veličković, Luka
AU  - Sibinčić, Nikolina
AU  - Stojadinović, Marija
AU  - Gligorijević, Nikola
AU  - Nikolić, Milan
AU  - Srdić, Tatjana
AU  - Minić, Simeon
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6052
AB  - This study investigates the impact of Porphyra sp. extracts on HT29 cell line growth and viability at reduced serum conditions. The concentration-dependent effects of phycobiliproteins (PBPs) on cell proliferation were examined over various time intervals. Lower concentrations of PBPs (20 μg/mL) demonstrated an increase in HT29 cell viability after 48 hours and 5 days of cultivation at reduced serum concentration (final serum concentration was in the range from 5 to 8%). This suggests a potential positive influence on cell proliferation, likely due to their antioxidant properties. Conversely, higher concentrations of PBPs exhibited inhibitory effects on cell growth, possibly due to cytotoxicity at elevated levels. Remarkably, when HT29 cells were cultured solely in algal extract without fetal calf serum (FCS), complete growth inhibition was observed after 72
hours. This finding underscores the insufficient nutrient and growth factor provision of PBPs alone for sustaining cell viability. Morphological differences observed in cells cultured with 70 μg/mL of PBPs indicated potential alterations in cellular morphology. Notably, 70 μg/mL of PBPs in RPMI medium with 5% FCS displayed growth inhibition compared to the control (5% FCS). Furthermore, we assessed HT29 cell adaptability to changes in FCS concentration and PBP supplementation. Cells incubated under varying FCS and PBP conditions were subcultured into RPMI medium with lower FCS concentration and PBPs from Porphyra. The viability of cells following subculturing indicated sustained adaptability to reduced FCS levels. Overall, this study provides valuable insights into the concentration-dependent effects of PBPs from Porphyra extracts
on HT29 cell growth and viability. The findings underscore the potential benefits of PBPs at lower concentrations for cell proliferation at reduced serum conditions and reveal the adaptability of HT29 cells to changing culture conditions.
PB  - Belgrade : Serbian Biochemical Society
C3  - "Biochemistry in Biotechnology", Twelfth Conference, International scientific meeting, September 21-23, 2023, Belgrade, Serbia
T1  - Exploring if Porphyra sp. extract functions as serum substitute in HT29 cell culture
SP  - 82
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6052
ER  - 

@conference{
author = "Veličković, Luka and Sibinčić, Nikolina and Stojadinović, Marija and Gligorijević, Nikola and Nikolić, Milan and Srdić, Tatjana and Minić, Simeon",
year = "2023",
abstract = "This study investigates the impact of Porphyra sp. extracts on HT29 cell line growth and viability at reduced serum conditions. The concentration-dependent effects of phycobiliproteins (PBPs) on cell proliferation were examined over various time intervals. Lower concentrations of PBPs (20 μg/mL) demonstrated an increase in HT29 cell viability after 48 hours and 5 days of cultivation at reduced serum concentration (final serum concentration was in the range from 5 to 8%). This suggests a potential positive influence on cell proliferation, likely due to their antioxidant properties. Conversely, higher concentrations of PBPs exhibited inhibitory effects on cell growth, possibly due to cytotoxicity at elevated levels. Remarkably, when HT29 cells were cultured solely in algal extract without fetal calf serum (FCS), complete growth inhibition was observed after 72
hours. This finding underscores the insufficient nutrient and growth factor provision of PBPs alone for sustaining cell viability. Morphological differences observed in cells cultured with 70 μg/mL of PBPs indicated potential alterations in cellular morphology. Notably, 70 μg/mL of PBPs in RPMI medium with 5% FCS displayed growth inhibition compared to the control (5% FCS). Furthermore, we assessed HT29 cell adaptability to changes in FCS concentration and PBP supplementation. Cells incubated under varying FCS and PBP conditions were subcultured into RPMI medium with lower FCS concentration and PBPs from Porphyra. The viability of cells following subculturing indicated sustained adaptability to reduced FCS levels. Overall, this study provides valuable insights into the concentration-dependent effects of PBPs from Porphyra extracts
on HT29 cell growth and viability. The findings underscore the potential benefits of PBPs at lower concentrations for cell proliferation at reduced serum conditions and reveal the adaptability of HT29 cells to changing culture conditions.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = ""Biochemistry in Biotechnology", Twelfth Conference, International scientific meeting, September 21-23, 2023, Belgrade, Serbia",
title = "Exploring if Porphyra sp. extract functions as serum substitute in HT29 cell culture",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6052"
}

Veličković, L., Sibinčić, N., Stojadinović, M., Gligorijević, N., Nikolić, M., Srdić, T.,& Minić, S.. (2023). Exploring if Porphyra sp. extract functions as serum substitute in HT29 cell culture. in "Biochemistry in Biotechnology", Twelfth Conference, International scientific meeting, September 21-23, 2023, Belgrade, Serbia
Belgrade : Serbian Biochemical Society., 82-82.
https://hdl.handle.net/21.15107/rcub_cherry_6052

Veličković L, Sibinčić N, Stojadinović M, Gligorijević N, Nikolić M, Srdić T, Minić S. Exploring if Porphyra sp. extract functions as serum substitute in HT29 cell culture. in "Biochemistry in Biotechnology", Twelfth Conference, International scientific meeting, September 21-23, 2023, Belgrade, Serbia. 2023;:82-82.
https://hdl.handle.net/21.15107/rcub_cherry_6052 .

Veličković, Luka, Sibinčić, Nikolina, Stojadinović, Marija, Gligorijević, Nikola, Nikolić, Milan, Srdić, Tatjana, Minić, Simeon, "Exploring if Porphyra sp. extract functions as serum substitute in HT29 cell culture" in "Biochemistry in Biotechnology", Twelfth Conference, International scientific meeting, September 21-23, 2023, Belgrade, Serbia (2023):82-82,
https://hdl.handle.net/21.15107/rcub_cherry_6052 .