TY  - JOUR
AU  - Samphao, Anchalee
AU  - Kunpatee, Kanjana
AU  - Prayoonpokarach, Sanchai
AU  - Wittayakun, Jatuporn
AU  - Šovrc, Ľubomír
AU  - Stanković, Dalibor
AU  - Zagar, Kristina
AU  - Ceh, Miran
AU  - Kalcher, Kurt
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2034
AB  - An ethanol biosensor based on alcohol dehydrogenase (ADH) attached to Au seeds decorated on magnetic nanoparticles (Fe3O4"Au NPs) is presented. ADH was immobilized on Fe3O4"Au NPs, which were subsequently fixed by a magnet on a carbon paste electrode modified with 5% (m:m) MnO2. Optimum conditions for the amperometric determination of ethanol with the biosensor were as follows: working potential +0.1 V (vs. Ag/AgCl); supporting electrolyte: 0.1 M phosphate buffer solution at pH 6.8 containing 0.25 mM of the coenzyme (NAD+); working electrode: carbon paste with magnetically attached Fe3O4"Au NPs (0.012 mg . cm(-2) electrode area) with immobilized alcohol dehydrogenase (120 units per cm(2) of electrode area). Linearity between signal and concentration was found for the range from 0.1 to 2.0 M ethanol (r(2) = 0.995) with a detection limit of 0.07 M, a sensitivity of 0.02 mu A . mM(-1) . cm(-2), a reproducibility of 4.0% RSD, and a repeatability of 2.7% RSD. The results for the determination of ethanol in alcoholic beverages showed good agreement with gas chromatography (GC) with recovery of 96.0-108.8%.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Electroanalysis
T1  - An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles
VL  - 27
IS  - 12
SP  - 2829
EP  - 2837
DO  - 10.1002/elan.201500315
ER  - 

@article{
author = "Samphao, Anchalee and Kunpatee, Kanjana and Prayoonpokarach, Sanchai and Wittayakun, Jatuporn and Šovrc, Ľubomír and Stanković, Dalibor and Zagar, Kristina and Ceh, Miran and Kalcher, Kurt",
year = "2015",
abstract = "An ethanol biosensor based on alcohol dehydrogenase (ADH) attached to Au seeds decorated on magnetic nanoparticles (Fe3O4"Au NPs) is presented. ADH was immobilized on Fe3O4"Au NPs, which were subsequently fixed by a magnet on a carbon paste electrode modified with 5% (m:m) MnO2. Optimum conditions for the amperometric determination of ethanol with the biosensor were as follows: working potential +0.1 V (vs. Ag/AgCl); supporting electrolyte: 0.1 M phosphate buffer solution at pH 6.8 containing 0.25 mM of the coenzyme (NAD+); working electrode: carbon paste with magnetically attached Fe3O4"Au NPs (0.012 mg . cm(-2) electrode area) with immobilized alcohol dehydrogenase (120 units per cm(2) of electrode area). Linearity between signal and concentration was found for the range from 0.1 to 2.0 M ethanol (r(2) = 0.995) with a detection limit of 0.07 M, a sensitivity of 0.02 mu A . mM(-1) . cm(-2), a reproducibility of 4.0% RSD, and a repeatability of 2.7% RSD. The results for the determination of ethanol in alcoholic beverages showed good agreement with gas chromatography (GC) with recovery of 96.0-108.8%.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Electroanalysis",
title = "An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles",
volume = "27",
number = "12",
pages = "2829-2837",
doi = "10.1002/elan.201500315"
}

Samphao, A., Kunpatee, K., Prayoonpokarach, S., Wittayakun, J., Šovrc, Ľ., Stanković, D., Zagar, K., Ceh, M.,& Kalcher, K.. (2015). An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. in Electroanalysis
Wiley-V C H Verlag Gmbh, Weinheim., 27(12), 2829-2837.
https://doi.org/10.1002/elan.201500315

Samphao A, Kunpatee K, Prayoonpokarach S, Wittayakun J, Šovrc Ľ, Stanković D, Zagar K, Ceh M, Kalcher K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. in Electroanalysis. 2015;27(12):2829-2837.
doi:10.1002/elan.201500315 .

Samphao, Anchalee, Kunpatee, Kanjana, Prayoonpokarach, Sanchai, Wittayakun, Jatuporn, Šovrc, Ľubomír, Stanković, Dalibor, Zagar, Kristina, Ceh, Miran, Kalcher, Kurt, "An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles" in Electroanalysis, 27, no. 12 (2015):2829-2837,
https://doi.org/10.1002/elan.201500315 . .

TY  - DATA
AU  - Samphao, Anchalee
AU  - Kunpatee, Kanjana
AU  - Prayoonpokarach, Sanchai
AU  - Wittayakun, Jatuporn
AU  - Šovrc, Ľubomír
AU  - Stanković, Dalibor
AU  - Zagar, Kristina
AU  - Ceh, Miran
AU  - Kalcher, Kurt
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3331
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Electroanalysis
T1  - Supplementary data for the article: Samphao, A.; Kunpatee, K.; Prayoonpokarach, S.; Wittayakun, J.; Švorc, L.; Stankovic, D. M.; Zagar, K.; Ceh, M.; Kalcher, K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. Electroanalysis 2015, 27 (12), 2829–2837. https://doi.org/10.1002/elan.201500315
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3331
ER  - 

@misc{
author = "Samphao, Anchalee and Kunpatee, Kanjana and Prayoonpokarach, Sanchai and Wittayakun, Jatuporn and Šovrc, Ľubomír and Stanković, Dalibor and Zagar, Kristina and Ceh, Miran and Kalcher, Kurt",
year = "2015",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Electroanalysis",
title = "Supplementary data for the article: Samphao, A.; Kunpatee, K.; Prayoonpokarach, S.; Wittayakun, J.; Švorc, L.; Stankovic, D. M.; Zagar, K.; Ceh, M.; Kalcher, K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. Electroanalysis 2015, 27 (12), 2829–2837. https://doi.org/10.1002/elan.201500315",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3331"
}

Samphao, A., Kunpatee, K., Prayoonpokarach, S., Wittayakun, J., Šovrc, Ľ., Stanković, D., Zagar, K., Ceh, M.,& Kalcher, K.. (2015). Supplementary data for the article: Samphao, A.; Kunpatee, K.; Prayoonpokarach, S.; Wittayakun, J.; Švorc, L.; Stankovic, D. M.; Zagar, K.; Ceh, M.; Kalcher, K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. Electroanalysis 2015, 27 (12), 2829–2837. https://doi.org/10.1002/elan.201500315. in Electroanalysis
Wiley-V C H Verlag Gmbh, Weinheim..
https://hdl.handle.net/21.15107/rcub_cherry_3331

Samphao A, Kunpatee K, Prayoonpokarach S, Wittayakun J, Šovrc Ľ, Stanković D, Zagar K, Ceh M, Kalcher K. Supplementary data for the article: Samphao, A.; Kunpatee, K.; Prayoonpokarach, S.; Wittayakun, J.; Švorc, L.; Stankovic, D. M.; Zagar, K.; Ceh, M.; Kalcher, K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. Electroanalysis 2015, 27 (12), 2829–2837. https://doi.org/10.1002/elan.201500315. in Electroanalysis. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3331 .

Samphao, Anchalee, Kunpatee, Kanjana, Prayoonpokarach, Sanchai, Wittayakun, Jatuporn, Šovrc, Ľubomír, Stanković, Dalibor, Zagar, Kristina, Ceh, Miran, Kalcher, Kurt, "Supplementary data for the article: Samphao, A.; Kunpatee, K.; Prayoonpokarach, S.; Wittayakun, J.; Švorc, L.; Stankovic, D. M.; Zagar, K.; Ceh, M.; Kalcher, K. An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4"Au Nanoparticles. Electroanalysis 2015, 27 (12), 2829–2837. https://doi.org/10.1002/elan.201500315" in Electroanalysis (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3331 .