Lozo, Jelena

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orcid::0000-0001-9888-5270
  • Lozo, Jelena (6)

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Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections

Dimkić, Ivica; Gobin, Ivana; Begić, Gabrijela; Repac Antić, Davorka; Ristivojević, Petar; Jurica, Karlo; Berić, Tanja; Lozo, Jelena; Abram, Maja; Stanković, Slaviša

(Elsevier GmbH, 2021)

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Gobin, Ivana
AU  - Begić, Gabrijela
AU  - Repac Antić, Davorka
AU  - Ristivojević, Petar
AU  - Jurica, Karlo
AU  - Berić, Tanja
AU  - Lozo, Jelena
AU  - Abram, Maja
AU  - Stanković, Slaviša
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S2210803321000257
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4525
AB  - Antibacterial and antibiofilm efficacy through a synergistic effect of herbal leaf extracts of bearberry (BE), bearberry tea (BTE), parsley (PE), and the strawberry tree (STE) was evaluated to discover an alternative approach to control urinary tract infections caused by Enterococci. UHPLC–DAD MS/MS analysis indicated that herbal extracts were rich in ellagic and gallic acids, catechin, quercetin, and arbutin, which are strong antioxidants. Mostly, Enterococcus faecalis isolates showed resistance only to gentamicin and/or norfloxacin (26.3 %) and moderate biofilm production (31.5 %), while 12 isolates of Enterococcus faecium showed multiple antibiotic resistance. The minimum inhibitory concentration for the majority of isolates was 1.25 mg ml−1 for STE, but 2.5 mg ml−1 for BE and BTE. Although individual extracts gave higher MIC values, for most isolates, a synergistic effect at lower concentrations was achieved, when BE or BTE (0.625 mg ml−1) was combined with STE (0.31 mg ml−1). The BTE extract was shown to have the strongest individual effect on initial adhesiveness and on biofilm formation to all selected isolates, while the synergism of BTE and STE caused significant biofilm inhibition at all concentrations tested. The synergistic effect achieved of the tested extract combinations might be a starting point in the development of alternative products, effective against antibiotic resistant Enterococcus isolates.
PB  - Elsevier GmbH
T2  - Journal of Herbal Medicine
T2  - Journal of Herbal MedicineJournal of Herbal Medicine
T1  - Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections
VL  - 28
SP  - 100445
DO  - 10.1016/j.hermed.2021.100445
ER  - 
@article{
author = "Dimkić, Ivica and Gobin, Ivana and Begić, Gabrijela and Repac Antić, Davorka and Ristivojević, Petar and Jurica, Karlo and Berić, Tanja and Lozo, Jelena and Abram, Maja and Stanković, Slaviša",
year = "2021",
abstract = "Antibacterial and antibiofilm efficacy through a synergistic effect of herbal leaf extracts of bearberry (BE), bearberry tea (BTE), parsley (PE), and the strawberry tree (STE) was evaluated to discover an alternative approach to control urinary tract infections caused by Enterococci. UHPLC–DAD MS/MS analysis indicated that herbal extracts were rich in ellagic and gallic acids, catechin, quercetin, and arbutin, which are strong antioxidants. Mostly, Enterococcus faecalis isolates showed resistance only to gentamicin and/or norfloxacin (26.3 %) and moderate biofilm production (31.5 %), while 12 isolates of Enterococcus faecium showed multiple antibiotic resistance. The minimum inhibitory concentration for the majority of isolates was 1.25 mg ml−1 for STE, but 2.5 mg ml−1 for BE and BTE. Although individual extracts gave higher MIC values, for most isolates, a synergistic effect at lower concentrations was achieved, when BE or BTE (0.625 mg ml−1) was combined with STE (0.31 mg ml−1). The BTE extract was shown to have the strongest individual effect on initial adhesiveness and on biofilm formation to all selected isolates, while the synergism of BTE and STE caused significant biofilm inhibition at all concentrations tested. The synergistic effect achieved of the tested extract combinations might be a starting point in the development of alternative products, effective against antibiotic resistant Enterococcus isolates.",
publisher = "Elsevier GmbH",
journal = "Journal of Herbal Medicine, Journal of Herbal MedicineJournal of Herbal Medicine",
title = "Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections",
volume = "28",
pages = "100445",
doi = "10.1016/j.hermed.2021.100445"
}
Dimkić, I., Gobin, I., Begić, G., Repac Antić, D., Ristivojević, P., Jurica, K., Berić, T., Lozo, J., Abram, M.,& Stanković, S.. (2021). Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections. in Journal of Herbal Medicine
Elsevier GmbH., 28, 100445.
https://doi.org/10.1016/j.hermed.2021.100445
Dimkić I, Gobin I, Begić G, Repac Antić D, Ristivojević P, Jurica K, Berić T, Lozo J, Abram M, Stanković S. Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections. in Journal of Herbal Medicine. 2021;28:100445.
doi:10.1016/j.hermed.2021.100445 .
Dimkić, Ivica, Gobin, Ivana, Begić, Gabrijela, Repac Antić, Davorka, Ristivojević, Petar, Jurica, Karlo, Berić, Tanja, Lozo, Jelena, Abram, Maja, Stanković, Slaviša, "Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections" in Journal of Herbal Medicine, 28 (2021):100445,
https://doi.org/10.1016/j.hermed.2021.100445 . .
4
4
3

Supplementary data for the article: Dimkić, I.; Gobin, I.; Begić, G.; Antić, D. R.; Ristivojević, P.; Jurica, K.; Berić, T.; Lozo, J.; Abram, M.; Stanković, S. Antibacterial Activity of Herbal Extracts towards Uropathogenic Enterococcus Isolates as a Natural Approach in Control of Urinary Tract Infections. Journal of Herbal Medicine 2021, 28, 100445. https://doi.org/10.1016/j.hermed.2021.100445.

Dimkić, Ivica; Gobin, Ivana; Begić, Gabrijela; Repac Antić, Davorka ; Ristivojević, Petar; Jurica, Karlo; Berić, Tanja; Lozo, Jelena; Abram, Maja; Stanković, Slaviša

(Elsevier GmbH, 2021)

TY  - DATA
AU  - Dimkić, Ivica
AU  - Gobin, Ivana
AU  - Begić, Gabrijela
AU  - Repac Antić, Davorka 
AU  - Ristivojević, Petar
AU  - Jurica, Karlo
AU  - Berić, Tanja
AU  - Lozo, Jelena
AU  - Abram, Maja
AU  - Stanković, Slaviša
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4526
PB  - Elsevier GmbH
T2  - Journal of Herbal Medicine
T1  - Supplementary data for the article: Dimkić, I.; Gobin, I.; Begić, G.; Antić, D. R.; Ristivojević, P.; Jurica, K.; Berić, T.; Lozo, J.; Abram, M.; Stanković, S. Antibacterial Activity of Herbal Extracts towards Uropathogenic Enterococcus Isolates as a Natural Approach in Control of Urinary Tract Infections. Journal of Herbal Medicine 2021, 28, 100445. https://doi.org/10.1016/j.hermed.2021.100445.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4526
ER  - 
@misc{
author = "Dimkić, Ivica and Gobin, Ivana and Begić, Gabrijela and Repac Antić, Davorka  and Ristivojević, Petar and Jurica, Karlo and Berić, Tanja and Lozo, Jelena and Abram, Maja and Stanković, Slaviša",
year = "2021",
publisher = "Elsevier GmbH",
journal = "Journal of Herbal Medicine",
title = "Supplementary data for the article: Dimkić, I.; Gobin, I.; Begić, G.; Antić, D. R.; Ristivojević, P.; Jurica, K.; Berić, T.; Lozo, J.; Abram, M.; Stanković, S. Antibacterial Activity of Herbal Extracts towards Uropathogenic Enterococcus Isolates as a Natural Approach in Control of Urinary Tract Infections. Journal of Herbal Medicine 2021, 28, 100445. https://doi.org/10.1016/j.hermed.2021.100445.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4526"
}
Dimkić, I., Gobin, I., Begić, G., Repac Antić, D., Ristivojević, P., Jurica, K., Berić, T., Lozo, J., Abram, M.,& Stanković, S.. (2021). Supplementary data for the article: Dimkić, I.; Gobin, I.; Begić, G.; Antić, D. R.; Ristivojević, P.; Jurica, K.; Berić, T.; Lozo, J.; Abram, M.; Stanković, S. Antibacterial Activity of Herbal Extracts towards Uropathogenic Enterococcus Isolates as a Natural Approach in Control of Urinary Tract Infections. Journal of Herbal Medicine 2021, 28, 100445. https://doi.org/10.1016/j.hermed.2021.100445.. in Journal of Herbal Medicine
Elsevier GmbH..
https://hdl.handle.net/21.15107/rcub_cherry_4526
Dimkić I, Gobin I, Begić G, Repac Antić D, Ristivojević P, Jurica K, Berić T, Lozo J, Abram M, Stanković S. Supplementary data for the article: Dimkić, I.; Gobin, I.; Begić, G.; Antić, D. R.; Ristivojević, P.; Jurica, K.; Berić, T.; Lozo, J.; Abram, M.; Stanković, S. Antibacterial Activity of Herbal Extracts towards Uropathogenic Enterococcus Isolates as a Natural Approach in Control of Urinary Tract Infections. Journal of Herbal Medicine 2021, 28, 100445. https://doi.org/10.1016/j.hermed.2021.100445.. in Journal of Herbal Medicine. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4526 .
Dimkić, Ivica, Gobin, Ivana, Begić, Gabrijela, Repac Antić, Davorka , Ristivojević, Petar, Jurica, Karlo, Berić, Tanja, Lozo, Jelena, Abram, Maja, Stanković, Slaviša, "Supplementary data for the article: Dimkić, I.; Gobin, I.; Begić, G.; Antić, D. R.; Ristivojević, P.; Jurica, K.; Berić, T.; Lozo, J.; Abram, M.; Stanković, S. Antibacterial Activity of Herbal Extracts towards Uropathogenic Enterococcus Isolates as a Natural Approach in Control of Urinary Tract Infections. Journal of Herbal Medicine 2021, 28, 100445. https://doi.org/10.1016/j.hermed.2021.100445." in Journal of Herbal Medicine (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4526 .

Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones

Klisurić, Olivera; Armaković, Sanja J.; Armaković, Stevan; Marković, Sanja B.; Todorović, Tamara; Portalone, Gustavo; Novović, Katarina; Lozo, Jelena; Filipović, Nenad R.

(Elsevier, 2020)

TY  - JOUR
AU  - Klisurić, Olivera
AU  - Armaković, Sanja J.
AU  - Armaković, Stevan
AU  - Marković, Sanja B.
AU  - Todorović, Tamara
AU  - Portalone, Gustavo
AU  - Novović, Katarina
AU  - Lozo, Jelena
AU  - Filipović, Nenad R.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3801
AB  - In this work pharmaceutical application of focused library of six quinoline-based chalcogensemicarbazones (QBCs) was tested through determination of their antimicrobial activity against twenty-eight Gram-negative and Gram-positive strains from different origin. Pharmacokinetic properties have been assessed by the analysis of frequently employed drug likeness parameters. Computational study has been complemented with calculation of their global and local reactive properties, within the framework of density functional theory (DFT). Among other information, DFT calculations helped us to locate the most reactive sites of investigated QBCs and to identify their sensitivity towards the oxidation.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones
VL  - 1203
SP  - 127482
DO  - 10.1016/j.molstruc.2019.127482
ER  - 
@article{
author = "Klisurić, Olivera and Armaković, Sanja J. and Armaković, Stevan and Marković, Sanja B. and Todorović, Tamara and Portalone, Gustavo and Novović, Katarina and Lozo, Jelena and Filipović, Nenad R.",
year = "2020",
abstract = "In this work pharmaceutical application of focused library of six quinoline-based chalcogensemicarbazones (QBCs) was tested through determination of their antimicrobial activity against twenty-eight Gram-negative and Gram-positive strains from different origin. Pharmacokinetic properties have been assessed by the analysis of frequently employed drug likeness parameters. Computational study has been complemented with calculation of their global and local reactive properties, within the framework of density functional theory (DFT). Among other information, DFT calculations helped us to locate the most reactive sites of investigated QBCs and to identify their sensitivity towards the oxidation.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones",
volume = "1203",
pages = "127482",
doi = "10.1016/j.molstruc.2019.127482"
}
Klisurić, O., Armaković, S. J., Armaković, S., Marković, S. B., Todorović, T., Portalone, G., Novović, K., Lozo, J.,& Filipović, N. R.. (2020). Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones. in Journal of Molecular Structure
Elsevier., 1203, 127482.
https://doi.org/10.1016/j.molstruc.2019.127482
Klisurić O, Armaković SJ, Armaković S, Marković SB, Todorović T, Portalone G, Novović K, Lozo J, Filipović NR. Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones. in Journal of Molecular Structure. 2020;1203:127482.
doi:10.1016/j.molstruc.2019.127482 .
Klisurić, Olivera, Armaković, Sanja J., Armaković, Stevan, Marković, Sanja B., Todorović, Tamara, Portalone, Gustavo, Novović, Katarina, Lozo, Jelena, Filipović, Nenad R., "Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones" in Journal of Molecular Structure, 1203 (2020):127482,
https://doi.org/10.1016/j.molstruc.2019.127482 . .
6
3
6
6

Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4

Lozo, Jelena; Mirković, Nemanja; O'Connor, Paula M.; Malešević, Milka; Miljković, Marija; Polović, Natalija; Jovčić, Branko; Cotter, Paul D.; Kojić, Milan O.

(Amer Soc Microbiology, Washington, 2017)

TY  - JOUR
AU  - Lozo, Jelena
AU  - Mirković, Nemanja
AU  - O'Connor, Paula M.
AU  - Malešević, Milka
AU  - Miljković, Marija
AU  - Polović, Natalija
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan O.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2541
AB  - Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4
VL  - 83
IS  - 21
DO  - 10.1128/AEM.01519-17
ER  - 
@article{
author = "Lozo, Jelena and Mirković, Nemanja and O'Connor, Paula M. and Malešević, Milka and Miljković, Marija and Polović, Natalija and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan O.",
year = "2017",
abstract = "Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4",
volume = "83",
number = "21",
doi = "10.1128/AEM.01519-17"
}
Lozo, J., Mirković, N., O'Connor, P. M., Malešević, M., Miljković, M., Polović, N., Jovčić, B., Cotter, P. D.,& Kojić, M. O.. (2017). Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 83(21).
https://doi.org/10.1128/AEM.01519-17
Lozo J, Mirković N, O'Connor PM, Malešević M, Miljković M, Polović N, Jovčić B, Cotter PD, Kojić MO. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4. in Applied and Environmental Microbiology. 2017;83(21).
doi:10.1128/AEM.01519-17 .
Lozo, Jelena, Mirković, Nemanja, O'Connor, Paula M., Malešević, Milka, Miljković, Marija, Polović, Natalija, Jovčić, Branko, Cotter, Paul D., Kojić, Milan O., "Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4" in Applied and Environmental Microbiology, 83, no. 21 (2017),
https://doi.org/10.1128/AEM.01519-17 . .
10
28
16
28
24

Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4

Lozo, Jelena; Mirković, Nemanja; O'Connor, Paula M.; Malešević, Milka; Miljković, Marija; Polović, Natalija; Jovčić, Branko; Cotter, Paul D.; Kojić, Milan O.

(Amer Soc Microbiology, Washington, 2017)

TY  - JOUR
AU  - Lozo, Jelena
AU  - Mirković, Nemanja
AU  - O'Connor, Paula M.
AU  - Malešević, Milka
AU  - Miljković, Marija
AU  - Polović, Natalija
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan O.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2998
AB  - Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4
VL  - 83
IS  - 21
DO  - 10.1128/AEM.01519-17
ER  - 
@article{
author = "Lozo, Jelena and Mirković, Nemanja and O'Connor, Paula M. and Malešević, Milka and Miljković, Marija and Polović, Natalija and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan O.",
year = "2017",
abstract = "Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4",
volume = "83",
number = "21",
doi = "10.1128/AEM.01519-17"
}
Lozo, J., Mirković, N., O'Connor, P. M., Malešević, M., Miljković, M., Polović, N., Jovčić, B., Cotter, P. D.,& Kojić, M. O.. (2017). Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 83(21).
https://doi.org/10.1128/AEM.01519-17
Lozo J, Mirković N, O'Connor PM, Malešević M, Miljković M, Polović N, Jovčić B, Cotter PD, Kojić MO. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4. in Applied and Environmental Microbiology. 2017;83(21).
doi:10.1128/AEM.01519-17 .
Lozo, Jelena, Mirković, Nemanja, O'Connor, Paula M., Malešević, Milka, Miljković, Marija, Polović, Natalija, Jovčić, Branko, Cotter, Paul D., Kojić, Milan O., "Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4" in Applied and Environmental Microbiology, 83, no. 21 (2017),
https://doi.org/10.1128/AEM.01519-17 . .
10
28
16
28
24

Supplementary data for article : Lozo, J.; Mirkovic, N.; O’Connor, P. M.; Malesevic, M.; Miljkovic, M.; Polović, N.; Jovcic, B.; Cotter, P. D.; Kojić, M. O. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus Lactis Subsp Lactis Bv. Diacetylactis BGBU1-4. Applied and Environmental Microbiology 2017, 83 (21). https://doi.org/10.1128/AEM.01519-17

Lozo, Jelena; Mirković, Nemanja; O'Connor, Paula M.; Malešević, Milka; Miljković, Marija; Polović, Natalija; Jovčić, Branko; Cotter, Paul D.; Kojić, Milan O.

(Amer Soc Microbiology, Washington, 2017)

TY  - DATA
AU  - Lozo, Jelena
AU  - Mirković, Nemanja
AU  - O'Connor, Paula M.
AU  - Malešević, Milka
AU  - Miljković, Marija
AU  - Polović, Natalija
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan O.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2999
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Supplementary data for article :  Lozo, J.; Mirkovic, N.; O’Connor, P. M.; Malesevic, M.; Miljkovic, M.; Polović, N.; Jovcic, B.; Cotter, P. D.; Kojić, M. O. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus Lactis Subsp Lactis Bv. Diacetylactis BGBU1-4. Applied and Environmental Microbiology 2017, 83 (21). https://doi.org/10.1128/AEM.01519-17
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2999
ER  - 
@misc{
author = "Lozo, Jelena and Mirković, Nemanja and O'Connor, Paula M. and Malešević, Milka and Miljković, Marija and Polović, Natalija and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan O.",
year = "2017",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Supplementary data for article :  Lozo, J.; Mirkovic, N.; O’Connor, P. M.; Malesevic, M.; Miljkovic, M.; Polović, N.; Jovcic, B.; Cotter, P. D.; Kojić, M. O. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus Lactis Subsp Lactis Bv. Diacetylactis BGBU1-4. Applied and Environmental Microbiology 2017, 83 (21). https://doi.org/10.1128/AEM.01519-17",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2999"
}
Lozo, J., Mirković, N., O'Connor, P. M., Malešević, M., Miljković, M., Polović, N., Jovčić, B., Cotter, P. D.,& Kojić, M. O.. (2017). Supplementary data for article :  Lozo, J.; Mirkovic, N.; O’Connor, P. M.; Malesevic, M.; Miljkovic, M.; Polović, N.; Jovcic, B.; Cotter, P. D.; Kojić, M. O. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus Lactis Subsp Lactis Bv. Diacetylactis BGBU1-4. Applied and Environmental Microbiology 2017, 83 (21). https://doi.org/10.1128/AEM.01519-17. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington..
https://hdl.handle.net/21.15107/rcub_cherry_2999
Lozo J, Mirković N, O'Connor PM, Malešević M, Miljković M, Polović N, Jovčić B, Cotter PD, Kojić MO. Supplementary data for article :  Lozo, J.; Mirkovic, N.; O’Connor, P. M.; Malesevic, M.; Miljkovic, M.; Polović, N.; Jovcic, B.; Cotter, P. D.; Kojić, M. O. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus Lactis Subsp Lactis Bv. Diacetylactis BGBU1-4. Applied and Environmental Microbiology 2017, 83 (21). https://doi.org/10.1128/AEM.01519-17. in Applied and Environmental Microbiology. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_2999 .
Lozo, Jelena, Mirković, Nemanja, O'Connor, Paula M., Malešević, Milka, Miljković, Marija, Polović, Natalija, Jovčić, Branko, Cotter, Paul D., Kojić, Milan O., "Supplementary data for article :  Lozo, J.; Mirkovic, N.; O’Connor, P. M.; Malesevic, M.; Miljkovic, M.; Polović, N.; Jovcic, B.; Cotter, P. D.; Kojić, M. O. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus Lactis Subsp Lactis Bv. Diacetylactis BGBU1-4. Applied and Environmental Microbiology 2017, 83 (21). https://doi.org/10.1128/AEM.01519-17" in Applied and Environmental Microbiology (2017),
https://hdl.handle.net/21.15107/rcub_cherry_2999 .