TY  - JOUR
AU  - Lazović, Mila
AU  - Ivković, Đurđa
AU  - Jankov, Milica
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6444
AB  - Due to the promising applications of propolis as a natural food preservative, functional ingredient or supplement, on one side, and the inherent toxicity and high volatility of organic solvents used for extraction of phenolics from propolis, a range of natural eutectic solvents (NESs) was evaluated for the extraction of phenolic compounds from poplar type propolis to create “green-labelled” food products. The phenolic profile of novel green propolis extracts was evaluated using high-performance thin-layer chromatography, while 16 phenolic compounds were quantified by ultra-high-performance liquid chromatography hyphenated with mass spectrometry. Pinocembrin and chrysin were the most dominant phenolics with an average concentration of 3111 and 3039 mg/kg in lactic acid-based NES, respectively. Biological activity, such as antioxidative and antimicrobial activities, were determined using spectrophotometric and well diffusion methods. Considering all spectrophotometric assays lactic acid-based presented as the best extraction medium for phenolic extraction, with TPC, TFC and RSA values 150.0 mg GAE/g, 190.0 mg RUE/g, and 132.8 mg TE/g on average, respectively. From 38 studied NESs, choline-chloride:tartaric acid (1:1 n/n), glycerol:lactic acid (1:1 n/n) and glycine: lactic acid (1:3 n/n) were the most promising extraction medium for obtaining the propolis extracts enriched with bioactive compounds. Additionally, proposed green solvents were proved to be the designer solvents and showed potential to be tuned to target specific phenolic compounds. Improved, or at least maintained, biological activity of NES propolis extracts support its application in agriculture industry.
PB  - Elsevier
T2  - Food Bioscience
T1  - Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals
VL  - 57
SP  - 103467
DO  - 10.1016/j.fbio.2023.103467
ER  - 

@article{
author = "Lazović, Mila and Ivković, Đurđa and Jankov, Milica and Dimkić, Ivica and Janakiev, Tamara and Trifković, Jelena and Milojković-Opsenica, Dušanka and Ristivojević, Petar",
year = "2024",
abstract = "Due to the promising applications of propolis as a natural food preservative, functional ingredient or supplement, on one side, and the inherent toxicity and high volatility of organic solvents used for extraction of phenolics from propolis, a range of natural eutectic solvents (NESs) was evaluated for the extraction of phenolic compounds from poplar type propolis to create “green-labelled” food products. The phenolic profile of novel green propolis extracts was evaluated using high-performance thin-layer chromatography, while 16 phenolic compounds were quantified by ultra-high-performance liquid chromatography hyphenated with mass spectrometry. Pinocembrin and chrysin were the most dominant phenolics with an average concentration of 3111 and 3039 mg/kg in lactic acid-based NES, respectively. Biological activity, such as antioxidative and antimicrobial activities, were determined using spectrophotometric and well diffusion methods. Considering all spectrophotometric assays lactic acid-based presented as the best extraction medium for phenolic extraction, with TPC, TFC and RSA values 150.0 mg GAE/g, 190.0 mg RUE/g, and 132.8 mg TE/g on average, respectively. From 38 studied NESs, choline-chloride:tartaric acid (1:1 n/n), glycerol:lactic acid (1:1 n/n) and glycine: lactic acid (1:3 n/n) were the most promising extraction medium for obtaining the propolis extracts enriched with bioactive compounds. Additionally, proposed green solvents were proved to be the designer solvents and showed potential to be tuned to target specific phenolic compounds. Improved, or at least maintained, biological activity of NES propolis extracts support its application in agriculture industry.",
publisher = "Elsevier",
journal = "Food Bioscience",
title = "Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals",
volume = "57",
pages = "103467",
doi = "10.1016/j.fbio.2023.103467"
}

Lazović, M., Ivković, Đ., Jankov, M., Dimkić, I., Janakiev, T., Trifković, J., Milojković-Opsenica, D.,& Ristivojević, P.. (2024). Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals. in Food Bioscience
Elsevier., 57, 103467.
https://doi.org/10.1016/j.fbio.2023.103467

Lazović M, Ivković Đ, Jankov M, Dimkić I, Janakiev T, Trifković J, Milojković-Opsenica D, Ristivojević P. Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals. in Food Bioscience. 2024;57:103467.
doi:10.1016/j.fbio.2023.103467 .

Lazović, Mila, Ivković, Đurđa, Jankov, Milica, Dimkić, Ivica, Janakiev, Tamara, Trifković, Jelena, Milojković-Opsenica, Dušanka, Ristivojević, Petar, "Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals" in Food Bioscience, 57 (2024):103467,
https://doi.org/10.1016/j.fbio.2023.103467 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag V.
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6270
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
SP  - 1148309
DO  - 10.3389/fevo.2023.1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag V. and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
pages = "1148309",
doi = "10.3389/fevo.2023.1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L. V., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić LV, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag V., Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Nenadić, Marija
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag V.
AU  - Milinčić, Katarina M.
AU  - Todosijević, Marina
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Ćurčić, Nina B.
AU  - Stevanović, Nataša
AU  - Mihajlović, Ljiljana
AU  - Vukoičić, Danijela Ž.
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6189
AB  - The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.
PB  - Frontiers Media
T2  - Frontiers in Ecology and Evolution
T1  - The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)
VL  - 11
DO  - 10.3389/fevo.2023.1120006
ER  - 

@article{
author = "Vesović, Nikola and Nenadić, Marija and Vranić, Sofija and Vujisić, Ljubodrag V. and Milinčić, Katarina M. and Todosijević, Marina and Dimkić, Ivica and Janakiev, Tamara and Ćurčić, Nina B. and Stevanović, Nataša and Mihajlović, Ljiljana and Vukoičić, Danijela Ž. and Ćurčić, Srećko",
year = "2023",
abstract = "The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.",
publisher = "Frontiers Media",
journal = "Frontiers in Ecology and Evolution",
title = "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)",
volume = "11",
doi = "10.3389/fevo.2023.1120006"
}

Vesović, N., Nenadić, M., Vranić, S., Vujisić, L. V., Milinčić, K. M., Todosijević, M., Dimkić, I., Janakiev, T., Ćurčić, N. B., Stevanović, N., Mihajlović, L., Vukoičić, D. Ž.,& Ćurčić, S.. (2023). The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution
Frontiers Media., 11.
https://doi.org/10.3389/fevo.2023.1120006

Vesović N, Nenadić M, Vranić S, Vujisić LV, Milinčić KM, Todosijević M, Dimkić I, Janakiev T, Ćurčić NB, Stevanović N, Mihajlović L, Vukoičić DŽ, Ćurčić S. The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution. 2023;11.
doi:10.3389/fevo.2023.1120006 .

Vesović, Nikola, Nenadić, Marija, Vranić, Sofija, Vujisić, Ljubodrag V., Milinčić, Katarina M., Todosijević, Marina, Dimkić, Ivica, Janakiev, Tamara, Ćurčić, Nina B., Stevanović, Nataša, Mihajlović, Ljiljana, Vukoičić, Danijela Ž., Ćurčić, Srećko, "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)" in Frontiers in Ecology and Evolution, 11 (2023),
https://doi.org/10.3389/fevo.2023.1120006 . .

TY  - JOUR
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević-Grbić, Milica
AU  - Opsenica, Dejan M.
AU  - Gašić, Uroš M.
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3729
AB  - European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and is traditionally processed into slivovitz brandy. The brown rot disease caused by Monilinia laxa drastically reduces plum yield almost every year. Fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Čačanska Lepotica and Čačanska Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium, and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of eleven M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist P4/16_1, which significantly reduced mycelial growth of M. laxa, was identified as Pseudomonas synxantha. Further characterization of P4/16_1 revealed the production of volatile organic compounds and phenazine-1-carboxylic acid (PCA). Crude benzene extract of PCA exhibited 57–63% mycelial growth inhibition of M. laxa. LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is the first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for further investigation for potential use as a biocontrol agent against brown rot-causing fungi.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa
VL  - 10
DO  - 10.3389/fmicb.2019.02287
ER  - 

@article{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Opsenica, Dejan M. and Gašić, Uroš M. and Stanković, Slaviša and Berić, Tanja",
year = "2019",
abstract = "European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and is traditionally processed into slivovitz brandy. The brown rot disease caused by Monilinia laxa drastically reduces plum yield almost every year. Fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Čačanska Lepotica and Čačanska Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium, and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of eleven M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist P4/16_1, which significantly reduced mycelial growth of M. laxa, was identified as Pseudomonas synxantha. Further characterization of P4/16_1 revealed the production of volatile organic compounds and phenazine-1-carboxylic acid (PCA). Crude benzene extract of PCA exhibited 57–63% mycelial growth inhibition of M. laxa. LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is the first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for further investigation for potential use as a biocontrol agent against brown rot-causing fungi.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa",
volume = "10",
doi = "10.3389/fmicb.2019.02287"
}

Janakiev, T., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Opsenica, D. M., Gašić, U. M., Stanković, S.,& Berić, T.. (2019). Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa. in Frontiers in Microbiology
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fmicb.2019.02287

Janakiev T, Dimkić I, Unković N, Ljaljević-Grbić M, Opsenica DM, Gašić UM, Stanković S, Berić T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.02287 .

Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Opsenica, Dejan M., Gašić, Uroš M., Stanković, Slaviša, Berić, Tanja, "Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.02287 . .

TY  - DATA
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević-Grbić, Milica
AU  - Opsenica, Dejan M.
AU  - Gašić, Uroš M.
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3730
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3730
ER  - 

@misc{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Opsenica, Dejan M. and Gašić, Uroš M. and Stanković, Slaviša and Berić, Tanja",
year = "2019",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3730"
}

Janakiev, T., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Opsenica, D. M., Gašić, U. M., Stanković, S.,& Berić, T.. (2019). Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287. in Frontiers in Microbiology
Frontiers Media S.A.., 10.
https://hdl.handle.net/21.15107/rcub_cherry_3730

Janakiev T, Dimkić I, Unković N, Ljaljević-Grbić M, Opsenica DM, Gašić UM, Stanković S, Berić T. Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287. in Frontiers in Microbiology. 2019;10.
https://hdl.handle.net/21.15107/rcub_cherry_3730 .

Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Opsenica, Dejan M., Gašić, Uroš M., Stanković, Slaviša, Berić, Tanja, "Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287" in Frontiers in Microbiology, 10 (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3730 .

TY  - DATA
AU  - Dimkić, Ivica
AU  - Ristivojević, Petar
AU  - Janakiev, Tamara
AU  - Berić, Tanja
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3593
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3593
ER  - 

@misc{
author = "Dimkić, Ivica and Ristivojević, Petar and Janakiev, Tamara and Berić, Tanja and Trifković, Jelena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3593"
}

Dimkić, I., Ristivojević, P., Janakiev, T., Berić, T., Trifković, J., Milojković-Opsenica, D.,& Stanković, S.. (2016). Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3593

Dimkić I, Ristivojević P, Janakiev T, Berić T, Trifković J, Milojković-Opsenica D, Stanković S. Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065. in Industrial Crops and Products. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3593 .

Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065" in Industrial Crops and Products (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3593 .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Ristivojević, Petar
AU  - Janakiev, Tamara
AU  - Berić, Tanja
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2345
AB  - Extensive employment of plant resins and propolis, as an antiseptic agents dating from ancient times in numerous cultures indicating that it may have antimicrobial and other biological properties. Resins from deciduous trees from the Populus and Salix genera, several fruit trees from the genus Prunus and a few other species were analyzed for their phenolic composition and antibacterial activity. Phenolic profiling of the plant resins was performed by high-performance thin-layer chromatography (HPTLC) and ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry. Antimicrobial activity against seven bacterial species was determined by minimum inhibitory concentration (MIC) assays and bioautography. The synergism, additivism, and antagonism of phenolic compounds were used to define the nature and type of interactions. Populus spp. showed higher amounts of p-hydroxybenzoic acid, p-coumaric acid, caffeic acid, chrysin, apigenin, quercetin, pinocembrin, pinobanksin and galangin, which confirmed the botanical origin of the orange and blue types of propolis. In addition, Gram-positive bacteria exhibited high susceptibility to poplar samples while being resistant to samples from other origins. Cherry bud samples had high amounts of naringenin and showed strong activity against Bacillus subtilis and Listeria monocytogenes. The combinations of tested phenolics showed mainly additive or indifferent effects. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis
VL  - 94
SP  - 856
EP  - 871
DO  - 10.1016/j.indcrop.2016.09.065
ER  - 

@article{
author = "Dimkić, Ivica and Ristivojević, Petar and Janakiev, Tamara and Berić, Tanja and Trifković, Jelena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
abstract = "Extensive employment of plant resins and propolis, as an antiseptic agents dating from ancient times in numerous cultures indicating that it may have antimicrobial and other biological properties. Resins from deciduous trees from the Populus and Salix genera, several fruit trees from the genus Prunus and a few other species were analyzed for their phenolic composition and antibacterial activity. Phenolic profiling of the plant resins was performed by high-performance thin-layer chromatography (HPTLC) and ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry. Antimicrobial activity against seven bacterial species was determined by minimum inhibitory concentration (MIC) assays and bioautography. The synergism, additivism, and antagonism of phenolic compounds were used to define the nature and type of interactions. Populus spp. showed higher amounts of p-hydroxybenzoic acid, p-coumaric acid, caffeic acid, chrysin, apigenin, quercetin, pinocembrin, pinobanksin and galangin, which confirmed the botanical origin of the orange and blue types of propolis. In addition, Gram-positive bacteria exhibited high susceptibility to poplar samples while being resistant to samples from other origins. Cherry bud samples had high amounts of naringenin and showed strong activity against Bacillus subtilis and Listeria monocytogenes. The combinations of tested phenolics showed mainly additive or indifferent effects. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis",
volume = "94",
pages = "856-871",
doi = "10.1016/j.indcrop.2016.09.065"
}

Dimkić, I., Ristivojević, P., Janakiev, T., Berić, T., Trifković, J., Milojković-Opsenica, D.,& Stanković, S.. (2016). Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 94, 856-871.
https://doi.org/10.1016/j.indcrop.2016.09.065

Dimkić I, Ristivojević P, Janakiev T, Berić T, Trifković J, Milojković-Opsenica D, Stanković S. Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis. in Industrial Crops and Products. 2016;94:856-871.
doi:10.1016/j.indcrop.2016.09.065 .

Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis" in Industrial Crops and Products, 94 (2016):856-871,
https://doi.org/10.1016/j.indcrop.2016.09.065 . .