TY  - JOUR
AU  - Pavlović, Slađan Z.
AU  - Krizmanić, Imre I.
AU  - Borković-Mitić, Slavica S.
AU  - Stojsavljević, Aleksandar
AU  - Mitić, Bojan M.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4308
AB  - We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathioneS-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from twolocalities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We alsodetermined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations ofmanganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium(U). The obtained results show that animals from VSB had significantly lower weights and lengths thananimals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while theSH content was significantly lower in animals from VSB compared to those from AVS and ABP. Nocorrelations between trace-element concentrations in water and animal tissue were observed. We concludedthat the obtained results were more likely a consequence of the combination of developmental differencesand the effects of different habitat conditions, environmental and anthropogenic influences than ofconcentrations of trace elements in the water alone.
PB  - Belgrade : Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)
VL  - 72
IS  - 4
DO  - 10.2298/ABS200825043P
ER  - 

@article{
author = "Pavlović, Slađan Z. and Krizmanić, Imre I. and Borković-Mitić, Slavica S. and Stojsavljević, Aleksandar and Mitić, Bojan M.",
year = "2020",
abstract = "We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathioneS-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from twolocalities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We alsodetermined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations ofmanganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium(U). The obtained results show that animals from VSB had significantly lower weights and lengths thananimals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while theSH content was significantly lower in animals from VSB compared to those from AVS and ABP. Nocorrelations between trace-element concentrations in water and animal tissue were observed. We concludedthat the obtained results were more likely a consequence of the combination of developmental differencesand the effects of different habitat conditions, environmental and anthropogenic influences than ofconcentrations of trace elements in the water alone.",
publisher = "Belgrade : Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)",
volume = "72",
number = "4",
doi = "10.2298/ABS200825043P"
}

Pavlović, S. Z., Krizmanić, I. I., Borković-Mitić, S. S., Stojsavljević, A.,& Mitić, B. M.. (2020). A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences
Belgrade : Serbian Biological Society., 72(4).
https://doi.org/10.2298/ABS200825043P

Pavlović SZ, Krizmanić II, Borković-Mitić SS, Stojsavljević A, Mitić BM. A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences. 2020;72(4).
doi:10.2298/ABS200825043P .

Pavlović, Slađan Z., Krizmanić, Imre I., Borković-Mitić, Slavica S., Stojsavljević, Aleksandar, Mitić, Bojan M., "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)" in Archives of Biological Sciences, 72, no. 4 (2020),
https://doi.org/10.2298/ABS200825043P . .

TY  - JOUR
AU  - Prokić, Marko D.
AU  - Borković-Mitić, Slavica S.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2548
AB  - In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P. ridibundus and P. esculentus) inhabiting two localities (the Danube-Tisza-Danube canal and the Ponjavica River) with different levels of pollution during pre- and post-hibernation periods, respectively autumn and spring. Our results showed that even though there were differences in the concentrations of accumulated metals and AOS parameters between localities and species, the frogs displayed almost the same patterns of AOS variations during seasons, with a higher AOS response observed in spring. The parameters SH groups, GSH, GR and SOD had been contributed most rather than others. Our findings indicate that oxidative stress during the post-hibernation period was mainly caused by the organisms' recovery from hibernation, as the result of natural selection acting on the AOS, and that the accumulated metals did not significantly, modify the AOS response. The present study provides new insight into the biological and physiological cellular responses of frogs to arousal stress.
PB  - Elsevier Science Inc, New York
T2  - Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology
T1  - Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals
VL  - 202
SP  - 19
EP  - 25
DO  - 10.1016/j.cbpc.2017.07.006
ER  - 

@article{
author = "Prokić, Marko D. and Borković-Mitić, Slavica S. and Krizmanić, Imre I. and Mutić, Jelena and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2017",
abstract = "In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P. ridibundus and P. esculentus) inhabiting two localities (the Danube-Tisza-Danube canal and the Ponjavica River) with different levels of pollution during pre- and post-hibernation periods, respectively autumn and spring. Our results showed that even though there were differences in the concentrations of accumulated metals and AOS parameters between localities and species, the frogs displayed almost the same patterns of AOS variations during seasons, with a higher AOS response observed in spring. The parameters SH groups, GSH, GR and SOD had been contributed most rather than others. Our findings indicate that oxidative stress during the post-hibernation period was mainly caused by the organisms' recovery from hibernation, as the result of natural selection acting on the AOS, and that the accumulated metals did not significantly, modify the AOS response. The present study provides new insight into the biological and physiological cellular responses of frogs to arousal stress.",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology",
title = "Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals",
volume = "202",
pages = "19-25",
doi = "10.1016/j.cbpc.2017.07.006"
}

Prokić, M. D., Borković-Mitić, S. S., Krizmanić, I. I., Mutić, J., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2017). Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals. in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology
Elsevier Science Inc, New York., 202, 19-25.
https://doi.org/10.1016/j.cbpc.2017.07.006

Prokić MD, Borković-Mitić SS, Krizmanić II, Mutić J, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals. in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology. 2017;202:19-25.
doi:10.1016/j.cbpc.2017.07.006 .

Prokić, Marko D., Borković-Mitić, Slavica S., Krizmanić, Imre I., Mutić, Jelena, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals" in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology, 202 (2017):19-25,
https://doi.org/10.1016/j.cbpc.2017.07.006 . .

TY  - DATA
AU  - Prokić, Marko D.
AU  - Borković-Mitić, Slavica S.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2017
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2962
PB  - Elsevier Science Inc, New York
T2  - Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology
T1  - Supplementary data for article :              Prokic, M. D.; Borkovic-Mitic, S. S.; Krizmanic, I. I.; Mutić, J.; Gavric, J. P.; Despotovic, S. G.; Gavrilović, B. R.; Radovanovic, T. B.; Pavlovic, S. Z.; Saicic, Z. S. Oxidative Stress Parameters in Two Pelophylax Esculentus Complex Frogs during Pre- and Post-Hibernation: Arousal vs Heavy Metals. Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology 2017, 202, 19–25.https://doi.org/10.1016/j.cbpc.2017.07.00
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2962
ER  - 

@misc{
author = "Prokić, Marko D. and Borković-Mitić, Slavica S. and Krizmanić, Imre I. and Mutić, Jelena and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2017, 2017",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology",
title = "Supplementary data for article :              Prokic, M. D.; Borkovic-Mitic, S. S.; Krizmanic, I. I.; Mutić, J.; Gavric, J. P.; Despotovic, S. G.; Gavrilović, B. R.; Radovanovic, T. B.; Pavlovic, S. Z.; Saicic, Z. S. Oxidative Stress Parameters in Two Pelophylax Esculentus Complex Frogs during Pre- and Post-Hibernation: Arousal vs Heavy Metals. Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology 2017, 202, 19–25.https://doi.org/10.1016/j.cbpc.2017.07.00",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2962"
}

Prokić, M. D., Borković-Mitić, S. S., Krizmanić, I. I., Mutić, J., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2017). Supplementary data for article :              Prokic, M. D.; Borkovic-Mitic, S. S.; Krizmanic, I. I.; Mutić, J.; Gavric, J. P.; Despotovic, S. G.; Gavrilović, B. R.; Radovanovic, T. B.; Pavlovic, S. Z.; Saicic, Z. S. Oxidative Stress Parameters in Two Pelophylax Esculentus Complex Frogs during Pre- and Post-Hibernation: Arousal vs Heavy Metals. Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology 2017, 202, 19–25.https://doi.org/10.1016/j.cbpc.2017.07.00. in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology
Elsevier Science Inc, New York..
https://hdl.handle.net/21.15107/rcub_cherry_2962

Prokić MD, Borković-Mitić SS, Krizmanić II, Mutić J, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Supplementary data for article :              Prokic, M. D.; Borkovic-Mitic, S. S.; Krizmanic, I. I.; Mutić, J.; Gavric, J. P.; Despotovic, S. G.; Gavrilović, B. R.; Radovanovic, T. B.; Pavlovic, S. Z.; Saicic, Z. S. Oxidative Stress Parameters in Two Pelophylax Esculentus Complex Frogs during Pre- and Post-Hibernation: Arousal vs Heavy Metals. Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology 2017, 202, 19–25.https://doi.org/10.1016/j.cbpc.2017.07.00. in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_2962 .

Prokić, Marko D., Borković-Mitić, Slavica S., Krizmanić, Imre I., Mutić, Jelena, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Supplementary data for article :              Prokic, M. D.; Borkovic-Mitic, S. S.; Krizmanic, I. I.; Mutić, J.; Gavric, J. P.; Despotovic, S. G.; Gavrilović, B. R.; Radovanovic, T. B.; Pavlovic, S. Z.; Saicic, Z. S. Oxidative Stress Parameters in Two Pelophylax Esculentus Complex Frogs during Pre- and Post-Hibernation: Arousal vs Heavy Metals. Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology 2017, 202, 19–25.https://doi.org/10.1016/j.cbpc.2017.07.00" in Comparative Biochemistry and Physiology. C: Toxicology and Pharmacology (2017),
https://hdl.handle.net/21.15107/rcub_cherry_2962 .

TY  - JOUR
AU  - Prokić, Marko D.
AU  - Borković-Mitić, Slavica S.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Vukojević, Vesna
AU  - Nasia, Mohammed
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1909
AB  - Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases (ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Frog samples were collected at two sites (the Danube-Tisza-Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by different levels of metal pollution. Differences between the metal contents in different tissues showed that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn, while only the Fe concentration was lower. No significant differences between metal concentrations in muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Ecotoxicology and Environmental Safety
T1  - Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution
VL  - 128
SP  - 21
EP  - 29
DO  - 10.1016/j.ecoenv.2016.02.005
ER  - 

@article{
author = "Prokić, Marko D. and Borković-Mitić, Slavica S. and Krizmanić, Imre I. and Mutić, Jelena and Vukojević, Vesna and Nasia, Mohammed and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2016",
abstract = "Heavy metal pollution of the aquatic environment is of great concern worldwide. Heavy metals are capable of inducing oxidative stress by increasing the formation of reactive oxygen species (ROS), and directly affecting the antioxidant defense system (AOS) in living organisms. The frog Pelophylax kl. esculentus is a semiaquatic species with semipermeable skin and a complex lifecycle, and represents a potentially useful bioindicator organism. The aim of this study was to investigate the accumulation of several heavy metals (Cd, Co, Cr, Cu, Fe, Hg, Ni, Pb and Zn), and their effects on selected parameters of the AOS, including the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), phase II biotransformation enzyme glutathione-S-transferase (GST), the total glutathione (GSH) contents and sulfhydryl (SH) group concentrations, as well as cholinesterases (ChEs) activities in the liver, skin and muscle of P. kl. esculentus. Frog samples were collected at two sites (the Danube-Tisza-Danube canal (DTDC) and the river Ponjavica) in Serbia, which are characterized by different levels of metal pollution. Differences between the metal contents in different tissues showed that the skin of frogs from the DTDC accumulated statistically higher concentrations of Cd, Cu, Pb and Zn, while only the Fe concentration was lower. No significant differences between metal concentrations in muscle tissues of frogs from the DTDC and Ponjavica were observed. Examination of the parameters of the AOS revealed that frogs from the DTDC had higher concentrations of GSH in the liver and of SH groups in the skin and muscle, whereas the activities of the antioxidative enzymes SOD, GHS-Px and GR in the liver and of GR in the skin were lower than in frogs from the Ponjavica. The relationship between metal concentrations and AOS parameters showed the highest number of correlations with GSH, GR and CAT, and with Ni, Zn, Hg, Cr and Cd. Based on the results in this study, we concluded that increased concentrations of heavy metals in frog tissues can alter the AOS, which leads to higher concentrations of GSH and SH groups and lower activities of antioxidative enzymes. The response of the AOS to metal pollutants allowed us to make a distinction between different frog tissues, and to conclude that the liver and skin are more suitable for assessing metal-induced oxidative stress in frogs than muscle. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Ecotoxicology and Environmental Safety",
title = "Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution",
volume = "128",
pages = "21-29",
doi = "10.1016/j.ecoenv.2016.02.005"
}

Prokić, M. D., Borković-Mitić, S. S., Krizmanić, I. I., Mutić, J., Vukojević, V., Nasia, M., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2016). Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution. in Ecotoxicology and Environmental Safety
Academic Press Inc Elsevier Science, San Diego., 128, 21-29.
https://doi.org/10.1016/j.ecoenv.2016.02.005

Prokić MD, Borković-Mitić SS, Krizmanić II, Mutić J, Vukojević V, Nasia M, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution. in Ecotoxicology and Environmental Safety. 2016;128:21-29.
doi:10.1016/j.ecoenv.2016.02.005 .

Prokić, Marko D., Borković-Mitić, Slavica S., Krizmanić, Imre I., Mutić, Jelena, Vukojević, Vesna, Nasia, Mohammed, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Antioxidative responses of the tissues of two wild populations of Pelophylax kl. esculentus frogs to heavy metal pollution" in Ecotoxicology and Environmental Safety, 128 (2016):21-29,
https://doi.org/10.1016/j.ecoenv.2016.02.005 . .

TY  - JOUR
AU  - Borković-Mitić, Slavica S.
AU  - Prokić, Marko D.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1941
AB  - To understand the effect of metals on the marsh frog Pelophylax ridibundus and the possible environment-induced changes in oxidative stress enzymes, we determined the concentrations of 18 metals: Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Ga, Hg, In, Li, Mn, Ni, Pb, Sr, and Zn, in the tissues (liver, skin, and muscle) and water samples collected from different locations in Serbia. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST), acetylcholinesterase (AChE), and changes in concentrations of reduced glutathione (GSH) and sulfhydryl groups (SH) were analyzed in the tissues of the sampled frogs. The concentrations of Al, Cd, Co, Cr, Cu, Fe, Ga, Hg, and Ni were highest in the liver, whereas those of Ba, Ca, Li, Mn, Pb, Sr, and Zn were highest in the skin. Hg correlated positively with liver SOD (in frogs from Danube-Tisza-Danube Canal (DTD)), muscle CAT (DTD), and muscle GST Ponjavica River (PO); Pb demonstrated a strong positive correlation with liver GR in frogs from Mt. Fruka Gora (FG); Cd only exhibited a positive correlation with AChE in the skin of frogs from DTD. In the skin, Zn correlated positively with AChE (DTD), SH groups (PO), and CAT (FG), and negatively with CAT, GST, and SH in the liver of frogs from DTD. Examination of these oxidative stress biomarkers, together with analysis of metal accumulation in the liver and skin of marsh frogs, provides a powerful tool for the assessment of metal pollution.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus)
VL  - 23
IS  - 10
SP  - 9649
EP  - 9659
DO  - 10.1007/s11356-016-6194-3
ER  - 

@article{
author = "Borković-Mitić, Slavica S. and Prokić, Marko D. and Krizmanić, Imre I. and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2016",
abstract = "To understand the effect of metals on the marsh frog Pelophylax ridibundus and the possible environment-induced changes in oxidative stress enzymes, we determined the concentrations of 18 metals: Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Ga, Hg, In, Li, Mn, Ni, Pb, Sr, and Zn, in the tissues (liver, skin, and muscle) and water samples collected from different locations in Serbia. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST), acetylcholinesterase (AChE), and changes in concentrations of reduced glutathione (GSH) and sulfhydryl groups (SH) were analyzed in the tissues of the sampled frogs. The concentrations of Al, Cd, Co, Cr, Cu, Fe, Ga, Hg, and Ni were highest in the liver, whereas those of Ba, Ca, Li, Mn, Pb, Sr, and Zn were highest in the skin. Hg correlated positively with liver SOD (in frogs from Danube-Tisza-Danube Canal (DTD)), muscle CAT (DTD), and muscle GST Ponjavica River (PO); Pb demonstrated a strong positive correlation with liver GR in frogs from Mt. Fruka Gora (FG); Cd only exhibited a positive correlation with AChE in the skin of frogs from DTD. In the skin, Zn correlated positively with AChE (DTD), SH groups (PO), and CAT (FG), and negatively with CAT, GST, and SH in the liver of frogs from DTD. Examination of these oxidative stress biomarkers, together with analysis of metal accumulation in the liver and skin of marsh frogs, provides a powerful tool for the assessment of metal pollution.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus)",
volume = "23",
number = "10",
pages = "9649-9659",
doi = "10.1007/s11356-016-6194-3"
}

Borković-Mitić, S. S., Prokić, M. D., Krizmanić, I. I., Mutić, J., Trifković, J., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2016). Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(10), 9649-9659.
https://doi.org/10.1007/s11356-016-6194-3

Borković-Mitić SS, Prokić MD, Krizmanić II, Mutić J, Trifković J, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus). in Environmental Science and Pollution Research. 2016;23(10):9649-9659.
doi:10.1007/s11356-016-6194-3 .

Borković-Mitić, Slavica S., Prokić, Marko D., Krizmanić, Imre I., Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus)" in Environmental Science and Pollution Research, 23, no. 10 (2016):9649-9659,
https://doi.org/10.1007/s11356-016-6194-3 . .

TY  - JOUR
AU  - Prokić, Marko D.
AU  - Borković-Mitić, Slavica S.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2340
AB  - Metals are involved in the formation of reactive oxygen species and can induce oxidative stress. The aim of this study was to assess the effects of several metals on oxidative stress in the skin and muscle of the Pelophylax esculentus "complex" frogs (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in Serbia, and the potential use of these species as bioindicator organisms in biomonitoring studies. The biomarkers of oxidative stress (SOD, CAT, GSH-Px, GR, GST activities and GSH, SH concentrations) and cholinesterase activity were investigated. The concentrations of nine metals (Fe, Cu, Zn, As, Cd, Cr, Hg, Ni, and Pb) were measured in the water and tissues. Correlations were established between metals and biomarkers in the tissues. The results of metal accumulation distinguished the skin of P. lessonae and muscle of P. ridibundus from other P. esculentus complex species. The oxidative stress biomarkers observed in P. ridibundus and P. esculentus had greater similarity than in P. lessonae. The P. lessonae displayed the highest number of correlations between biomarkers and metals. The results of tissue responses revealed that skin was more susceptible to metal-induced oxidative stress, with only exception of As. In the light of these findings, we can suggest the use of P. esculentus complex species as a biomonitoring species in studies of metal accumulation and metal-induced oxidative stress, but with special emphasis on P. lessonae.
PB  - Springer, Dordrecht
T2  - Ecotoxicology
T1  - Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex
VL  - 25
IS  - 8
SP  - 1531
EP  - 1542
DO  - 10.1007/s10646-016-1707-x
ER  - 

@article{
author = "Prokić, Marko D. and Borković-Mitić, Slavica S. and Krizmanić, Imre I. and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2016",
abstract = "Metals are involved in the formation of reactive oxygen species and can induce oxidative stress. The aim of this study was to assess the effects of several metals on oxidative stress in the skin and muscle of the Pelophylax esculentus "complex" frogs (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in Serbia, and the potential use of these species as bioindicator organisms in biomonitoring studies. The biomarkers of oxidative stress (SOD, CAT, GSH-Px, GR, GST activities and GSH, SH concentrations) and cholinesterase activity were investigated. The concentrations of nine metals (Fe, Cu, Zn, As, Cd, Cr, Hg, Ni, and Pb) were measured in the water and tissues. Correlations were established between metals and biomarkers in the tissues. The results of metal accumulation distinguished the skin of P. lessonae and muscle of P. ridibundus from other P. esculentus complex species. The oxidative stress biomarkers observed in P. ridibundus and P. esculentus had greater similarity than in P. lessonae. The P. lessonae displayed the highest number of correlations between biomarkers and metals. The results of tissue responses revealed that skin was more susceptible to metal-induced oxidative stress, with only exception of As. In the light of these findings, we can suggest the use of P. esculentus complex species as a biomonitoring species in studies of metal accumulation and metal-induced oxidative stress, but with special emphasis on P. lessonae.",
publisher = "Springer, Dordrecht",
journal = "Ecotoxicology",
title = "Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex",
volume = "25",
number = "8",
pages = "1531-1542",
doi = "10.1007/s10646-016-1707-x"
}

Prokić, M. D., Borković-Mitić, S. S., Krizmanić, I. I., Mutić, J., Trifković, J., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2016). Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. in Ecotoxicology
Springer, Dordrecht., 25(8), 1531-1542.
https://doi.org/10.1007/s10646-016-1707-x

Prokić MD, Borković-Mitić SS, Krizmanić II, Mutić J, Trifković J, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. in Ecotoxicology. 2016;25(8):1531-1542.
doi:10.1007/s10646-016-1707-x .

Prokić, Marko D., Borković-Mitić, Slavica S., Krizmanić, Imre I., Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex" in Ecotoxicology, 25, no. 8 (2016):1531-1542,
https://doi.org/10.1007/s10646-016-1707-x . .

TY  - DATA
AU  - Prokić, Marko D.
AU  - Borković-Mitić, Slavica S.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3391
PB  - Springer, Dordrecht
T2  - Ecotoxicology
T1  - Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3391
ER  - 

@misc{
author = "Prokić, Marko D. and Borković-Mitić, Slavica S. and Krizmanić, Imre I. and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2016",
publisher = "Springer, Dordrecht",
journal = "Ecotoxicology",
title = "Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3391"
}

Prokić, M. D., Borković-Mitić, S. S., Krizmanić, I. I., Mutić, J., Trifković, J., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2016). Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x. in Ecotoxicology
Springer, Dordrecht..
https://hdl.handle.net/21.15107/rcub_cherry_3391

Prokić MD, Borković-Mitić SS, Krizmanić II, Mutić J, Trifković J, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x. in Ecotoxicology. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3391 .

Prokić, Marko D., Borković-Mitić, Slavica S., Krizmanić, Imre I., Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x" in Ecotoxicology (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3391 .

TY  - DATA
AU  - Borković-Mitić, Slavica S.
AU  - Prokić, Marko D.
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena P.
AU  - Despotović, Svetlana G.
AU  - Gavrilović, Branka R.
AU  - Radovanović, Tijana B.
AU  - Pavlović, Slađan Z.
AU  - Saičić, Zorica S.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3392
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Supplementary data for the article: Borković-Mitić, S. S.; Prokić, M. D.; Krizmanić, I. I.; Mutić, J.; Trifković, J.; Gavrić, J.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Biomarkers of Oxidative Stress and Metal Accumulation in Marsh Frog (Pelophylax Ridibundus). Environmental Science and Pollution Research 2016, 23 (10), 9649–9659. https://doi.org/10.1007/s11356-016-6194-3
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3392
ER  - 

@misc{
author = "Borković-Mitić, Slavica S. and Prokić, Marko D. and Krizmanić, Imre I. and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena P. and Despotović, Svetlana G. and Gavrilović, Branka R. and Radovanović, Tijana B. and Pavlović, Slađan Z. and Saičić, Zorica S.",
year = "2016",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Supplementary data for the article: Borković-Mitić, S. S.; Prokić, M. D.; Krizmanić, I. I.; Mutić, J.; Trifković, J.; Gavrić, J.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Biomarkers of Oxidative Stress and Metal Accumulation in Marsh Frog (Pelophylax Ridibundus). Environmental Science and Pollution Research 2016, 23 (10), 9649–9659. https://doi.org/10.1007/s11356-016-6194-3",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3392"
}

Borković-Mitić, S. S., Prokić, M. D., Krizmanić, I. I., Mutić, J., Trifković, J., Gavrić, J. P., Despotović, S. G., Gavrilović, B. R., Radovanović, T. B., Pavlović, S. Z.,& Saičić, Z. S.. (2016). Supplementary data for the article: Borković-Mitić, S. S.; Prokić, M. D.; Krizmanić, I. I.; Mutić, J.; Trifković, J.; Gavrić, J.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Biomarkers of Oxidative Stress and Metal Accumulation in Marsh Frog (Pelophylax Ridibundus). Environmental Science and Pollution Research 2016, 23 (10), 9649–9659. https://doi.org/10.1007/s11356-016-6194-3. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg..
https://hdl.handle.net/21.15107/rcub_cherry_3392

Borković-Mitić SS, Prokić MD, Krizmanić II, Mutić J, Trifković J, Gavrić JP, Despotović SG, Gavrilović BR, Radovanović TB, Pavlović SZ, Saičić ZS. Supplementary data for the article: Borković-Mitić, S. S.; Prokić, M. D.; Krizmanić, I. I.; Mutić, J.; Trifković, J.; Gavrić, J.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Biomarkers of Oxidative Stress and Metal Accumulation in Marsh Frog (Pelophylax Ridibundus). Environmental Science and Pollution Research 2016, 23 (10), 9649–9659. https://doi.org/10.1007/s11356-016-6194-3. in Environmental Science and Pollution Research. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3392 .

Borković-Mitić, Slavica S., Prokić, Marko D., Krizmanić, Imre I., Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena P., Despotović, Svetlana G., Gavrilović, Branka R., Radovanović, Tijana B., Pavlović, Slađan Z., Saičić, Zorica S., "Supplementary data for the article: Borković-Mitić, S. S.; Prokić, M. D.; Krizmanić, I. I.; Mutić, J.; Trifković, J.; Gavrić, J.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Biomarkers of Oxidative Stress and Metal Accumulation in Marsh Frog (Pelophylax Ridibundus). Environmental Science and Pollution Research 2016, 23 (10), 9649–9659. https://doi.org/10.1007/s11356-016-6194-3" in Environmental Science and Pollution Research (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3392 .