TY  - JOUR
AU  - Martinović, Biljana
AU  - Jelić, Ivana V.
AU  - Rikalović, Milena G.
AU  - Šljivić-Ivanović, Marija Z.
AU  - Radosavljević, Jelena
AU  - Kostić, Aleksandar Ž.
AU  - Adžemovic, Mesud
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4208
AB  - The aim of the present study is the comparison of ambient pollution in working environment during infectious medical waste treatment in two relevant health care institutions in the Republic of Serbia - The Blood Transfusion Institute of Serbia and The Clinical Centre of Serbia by different sterilization methods (sterilizer-convertor and autoclave, respectively). Monitoring and analysis of the following chemical compounds were performed in both institutions: water vapor, carbon dioxide, carbon monoxide, hydrochloric acid, methane, ethane, propane, hexane, formaldehyde, benzene, toluene, m-xylene, phenol, acrolein, ethanol, ethyl acetate, ethylamine, ethylene oxide, methanol, dimethyl disulphide, dimethyl sulphide, ethyl mercaptan, methyl mercaptan, freon 11 (trichlorofluoromethane), carbonyl sulphide and hydrogen chloride. The determination of vapor-phase concentrations was done by extractive Fourier transform infrared spectroscopy. Monitoring of ambient pollution during infectious medical waste sterilization has shown that mercaptans, acrolein, formaldehyde, dimethyl sulphide, and ethylene oxide are emitted in both health institutions, in concentrations which are not permitted by regulations, while increased concentrations of hydrogen chloride and phenol were found in the Clinical Centre of Serbia. A comparison of ambient pollution with two different sterilization methods has shown that higher concentrations of pollutants are emitted at higher temperatures. Considering the fact that mentioned compounds exhibit high toxicity, hence represent the risk to air quality in working and living environment, i.e. represents a risk to human health it is necessary to revise and improve the existing treatment methods.
PB  - Parlar Scientific Publications
T2  - Fresenius Environmental Bulletin
T1  - Comparison of air pollution in the working environment during in site treatment of infectious medical waste by convertor and autoclave sterilization
VL  - 29
IS  - 4
SP  - 2160
EP  - 2165
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4208
ER  - 

@article{
author = "Martinović, Biljana and Jelić, Ivana V. and Rikalović, Milena G. and Šljivić-Ivanović, Marija Z. and Radosavljević, Jelena and Kostić, Aleksandar Ž. and Adžemovic, Mesud",
year = "2020",
abstract = "The aim of the present study is the comparison of ambient pollution in working environment during infectious medical waste treatment in two relevant health care institutions in the Republic of Serbia - The Blood Transfusion Institute of Serbia and The Clinical Centre of Serbia by different sterilization methods (sterilizer-convertor and autoclave, respectively). Monitoring and analysis of the following chemical compounds were performed in both institutions: water vapor, carbon dioxide, carbon monoxide, hydrochloric acid, methane, ethane, propane, hexane, formaldehyde, benzene, toluene, m-xylene, phenol, acrolein, ethanol, ethyl acetate, ethylamine, ethylene oxide, methanol, dimethyl disulphide, dimethyl sulphide, ethyl mercaptan, methyl mercaptan, freon 11 (trichlorofluoromethane), carbonyl sulphide and hydrogen chloride. The determination of vapor-phase concentrations was done by extractive Fourier transform infrared spectroscopy. Monitoring of ambient pollution during infectious medical waste sterilization has shown that mercaptans, acrolein, formaldehyde, dimethyl sulphide, and ethylene oxide are emitted in both health institutions, in concentrations which are not permitted by regulations, while increased concentrations of hydrogen chloride and phenol were found in the Clinical Centre of Serbia. A comparison of ambient pollution with two different sterilization methods has shown that higher concentrations of pollutants are emitted at higher temperatures. Considering the fact that mentioned compounds exhibit high toxicity, hence represent the risk to air quality in working and living environment, i.e. represents a risk to human health it is necessary to revise and improve the existing treatment methods.",
publisher = "Parlar Scientific Publications",
journal = "Fresenius Environmental Bulletin",
title = "Comparison of air pollution in the working environment during in site treatment of infectious medical waste by convertor and autoclave sterilization",
volume = "29",
number = "4",
pages = "2160-2165",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4208"
}

Martinović, B., Jelić, I. V., Rikalović, M. G., Šljivić-Ivanović, M. Z., Radosavljević, J., Kostić, A. Ž.,& Adžemovic, M.. (2020). Comparison of air pollution in the working environment during in site treatment of infectious medical waste by convertor and autoclave sterilization. in Fresenius Environmental Bulletin
Parlar Scientific Publications., 29(4), 2160-2165.
https://hdl.handle.net/21.15107/rcub_cherry_4208

Martinović B, Jelić IV, Rikalović MG, Šljivić-Ivanović MZ, Radosavljević J, Kostić AŽ, Adžemovic M. Comparison of air pollution in the working environment during in site treatment of infectious medical waste by convertor and autoclave sterilization. in Fresenius Environmental Bulletin. 2020;29(4):2160-2165.
https://hdl.handle.net/21.15107/rcub_cherry_4208 .

Martinović, Biljana, Jelić, Ivana V., Rikalović, Milena G., Šljivić-Ivanović, Marija Z., Radosavljević, Jelena, Kostić, Aleksandar Ž., Adžemovic, Mesud, "Comparison of air pollution in the working environment during in site treatment of infectious medical waste by convertor and autoclave sterilization" in Fresenius Environmental Bulletin, 29, no. 4 (2020):2160-2165,
https://hdl.handle.net/21.15107/rcub_cherry_4208 .

TY  - JOUR
AU  - Izrael-Živković, Lidija
AU  - Beškoski, Vladimir
AU  - Rikalović, Milena G.
AU  - Kazazić, Snježana
AU  - Shapiro, Nicole
AU  - Woyke, Tanja
AU  - Gojgić-Cvijović, Gordana D.
AU  - Vrvić, Miroslav M.
AU  - Maksimović, Nela
AU  - Karadžić, Ivanka M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3705
AB  - The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions and involved in catabolism of toxic aromatic organic compounds were identified. P. aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100 mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200 mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.
PB  - Springer Japan
T2  - Extremophiles
T1  - High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals
VL  - 23
IS  - 4
SP  - 399
EP  - 405
DO  - 10.1007/s00792-019-01092-w
ER  - 

@article{
author = "Izrael-Živković, Lidija and Beškoski, Vladimir and Rikalović, Milena G. and Kazazić, Snježana and Shapiro, Nicole and Woyke, Tanja and Gojgić-Cvijović, Gordana D. and Vrvić, Miroslav M. and Maksimović, Nela and Karadžić, Ivanka M.",
year = "2019",
abstract = "The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions and involved in catabolism of toxic aromatic organic compounds were identified. P. aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100 mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200 mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.",
publisher = "Springer Japan",
journal = "Extremophiles",
title = "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals",
volume = "23",
number = "4",
pages = "399-405",
doi = "10.1007/s00792-019-01092-w"
}

Izrael-Živković, L., Beškoski, V., Rikalović, M. G., Kazazić, S., Shapiro, N., Woyke, T., Gojgić-Cvijović, G. D., Vrvić, M. M., Maksimović, N.,& Karadžić, I. M.. (2019). High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles
Springer Japan., 23(4), 399-405.
https://doi.org/10.1007/s00792-019-01092-w

Izrael-Živković L, Beškoski V, Rikalović MG, Kazazić S, Shapiro N, Woyke T, Gojgić-Cvijović GD, Vrvić MM, Maksimović N, Karadžić IM. High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles. 2019;23(4):399-405.
doi:10.1007/s00792-019-01092-w .

Izrael-Živković, Lidija, Beškoski, Vladimir, Rikalović, Milena G., Kazazić, Snježana, Shapiro, Nicole, Woyke, Tanja, Gojgić-Cvijović, Gordana D., Vrvić, Miroslav M., Maksimović, Nela, Karadžić, Ivanka M., "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals" in Extremophiles, 23, no. 4 (2019):399-405,
https://doi.org/10.1007/s00792-019-01092-w . .

TY  - DATA
AU  - Beškoski, Vladimir
AU  - Rikalović, Milena G.
AU  - Kazazić, Snježana
AU  - Shapiro, Nicole
AU  - Woyke, Tanja
AU  - Gojgić-Cvijović, Gordana D.
AU  - Vrvić, Miroslav M.
AU  - Maksimović, Nela
AU  - Karadžić, Ivanka M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3706
PB  - Springer Japan
T2  - Extremophiles
T1  - Supplementary data for the article: Izrael-Živković, L.; Beškoski, V.; Rikalović, M.; Kazazić, S.; Shapiro, N.; Woyke, T.; Gojgić-Cvijović, G.; Vrvić, M. M.; Maksimović, N.; Karadžić, I. High-Quality Draft Genome Sequence of Pseudomonas Aeruginosa San Ai, an Environmental Isolate Resistant to Heavy Metals. Extremophiles 2019, 23 (4), 399–405. https://doi.org/10.1007/s00792-019-01092-w
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3706
ER  - 

@misc{
author = "Beškoski, Vladimir and Rikalović, Milena G. and Kazazić, Snježana and Shapiro, Nicole and Woyke, Tanja and Gojgić-Cvijović, Gordana D. and Vrvić, Miroslav M. and Maksimović, Nela and Karadžić, Ivanka M.",
year = "2019",
publisher = "Springer Japan",
journal = "Extremophiles",
title = "Supplementary data for the article: Izrael-Živković, L.; Beškoski, V.; Rikalović, M.; Kazazić, S.; Shapiro, N.; Woyke, T.; Gojgić-Cvijović, G.; Vrvić, M. M.; Maksimović, N.; Karadžić, I. High-Quality Draft Genome Sequence of Pseudomonas Aeruginosa San Ai, an Environmental Isolate Resistant to Heavy Metals. Extremophiles 2019, 23 (4), 399–405. https://doi.org/10.1007/s00792-019-01092-w",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3706"
}

Beškoski, V., Rikalović, M. G., Kazazić, S., Shapiro, N., Woyke, T., Gojgić-Cvijović, G. D., Vrvić, M. M., Maksimović, N.,& Karadžić, I. M.. (2019). Supplementary data for the article: Izrael-Živković, L.; Beškoski, V.; Rikalović, M.; Kazazić, S.; Shapiro, N.; Woyke, T.; Gojgić-Cvijović, G.; Vrvić, M. M.; Maksimović, N.; Karadžić, I. High-Quality Draft Genome Sequence of Pseudomonas Aeruginosa San Ai, an Environmental Isolate Resistant to Heavy Metals. Extremophiles 2019, 23 (4), 399–405. https://doi.org/10.1007/s00792-019-01092-w. in Extremophiles
Springer Japan..
https://hdl.handle.net/21.15107/rcub_cherry_3706

Beškoski V, Rikalović MG, Kazazić S, Shapiro N, Woyke T, Gojgić-Cvijović GD, Vrvić MM, Maksimović N, Karadžić IM. Supplementary data for the article: Izrael-Živković, L.; Beškoski, V.; Rikalović, M.; Kazazić, S.; Shapiro, N.; Woyke, T.; Gojgić-Cvijović, G.; Vrvić, M. M.; Maksimović, N.; Karadžić, I. High-Quality Draft Genome Sequence of Pseudomonas Aeruginosa San Ai, an Environmental Isolate Resistant to Heavy Metals. Extremophiles 2019, 23 (4), 399–405. https://doi.org/10.1007/s00792-019-01092-w. in Extremophiles. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3706 .

Beškoski, Vladimir, Rikalović, Milena G., Kazazić, Snježana, Shapiro, Nicole, Woyke, Tanja, Gojgić-Cvijović, Gordana D., Vrvić, Miroslav M., Maksimović, Nela, Karadžić, Ivanka M., "Supplementary data for the article: Izrael-Živković, L.; Beškoski, V.; Rikalović, M.; Kazazić, S.; Shapiro, N.; Woyke, T.; Gojgić-Cvijović, G.; Vrvić, M. M.; Maksimović, N.; Karadžić, I. High-Quality Draft Genome Sequence of Pseudomonas Aeruginosa San Ai, an Environmental Isolate Resistant to Heavy Metals. Extremophiles 2019, 23 (4), 399–405. https://doi.org/10.1007/s00792-019-01092-w" in Extremophiles (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3706 .

TY  - JOUR
AU  - Izrael-Živković, Lidija
AU  - Rikalović, Milena G.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Kazazić, Saša
AU  - Vrvić, Miroslav M.
AU  - Brčeski, Ilija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka D.
AU  - Gopčević, Kristina
AU  - Karadžić, Ivanka M.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2118
AB  - Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai
VL  - 8
IS  - 19
SP  - 10549
EP  - 10560
DO  - 10.1039/c8ra00371h
ER  - 

@article{
author = "Izrael-Živković, Lidija and Rikalović, Milena G. and Gojgić-Cvijović, Gordana D. and Kazazić, Saša and Vrvić, Miroslav M. and Brčeski, Ilija and Beškoski, Vladimir and Lončarević, Branka D. and Gopčević, Kristina and Karadžić, Ivanka M.",
year = "2018",
abstract = "Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai",
volume = "8",
number = "19",
pages = "10549-10560",
doi = "10.1039/c8ra00371h"
}

Izrael-Živković, L., Rikalović, M. G., Gojgić-Cvijović, G. D., Kazazić, S., Vrvić, M. M., Brčeski, I., Beškoski, V., Lončarević, B. D., Gopčević, K.,& Karadžić, I. M.. (2018). Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. in RSC Advances
Royal Soc Chemistry, Cambridge., 8(19), 10549-10560.
https://doi.org/10.1039/c8ra00371h

Izrael-Živković L, Rikalović MG, Gojgić-Cvijović GD, Kazazić S, Vrvić MM, Brčeski I, Beškoski V, Lončarević BD, Gopčević K, Karadžić IM. Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. in RSC Advances. 2018;8(19):10549-10560.
doi:10.1039/c8ra00371h .

Izrael-Živković, Lidija, Rikalović, Milena G., Gojgić-Cvijović, Gordana D., Kazazić, Saša, Vrvić, Miroslav M., Brčeski, Ilija, Beškoski, Vladimir, Lončarević, Branka D., Gopčević, Kristina, Karadžić, Ivanka M., "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai" in RSC Advances, 8, no. 19 (2018):10549-10560,
https://doi.org/10.1039/c8ra00371h . .

TY  - DATA
AU  - Izrael-Živković, Lidija
AU  - Rikalović, Milena G.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Kazazić, Saša
AU  - Vrvić, Miroslav M.
AU  - Brčeski, Ilija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka D.
AU  - Gopčević, Kristina
AU  - Karadžić, Ivanka M.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3179
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3179
ER  - 

@misc{
author = "Izrael-Živković, Lidija and Rikalović, Milena G. and Gojgić-Cvijović, Gordana D. and Kazazić, Saša and Vrvić, Miroslav M. and Brčeski, Ilija and Beškoski, Vladimir and Lončarević, Branka D. and Gopčević, Kristina and Karadžić, Ivanka M.",
year = "2018",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3179"
}

Izrael-Živković, L., Rikalović, M. G., Gojgić-Cvijović, G. D., Kazazić, S., Vrvić, M. M., Brčeski, I., Beškoski, V., Lončarević, B. D., Gopčević, K.,& Karadžić, I. M.. (2018). Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h. in RSC Advances
Royal Soc Chemistry, Cambridge..
https://hdl.handle.net/21.15107/rcub_cherry_3179

Izrael-Živković L, Rikalović MG, Gojgić-Cvijović GD, Kazazić S, Vrvić MM, Brčeski I, Beškoski V, Lončarević BD, Gopčević K, Karadžić IM. Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h. in RSC Advances. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3179 .

Izrael-Živković, Lidija, Rikalović, Milena G., Gojgić-Cvijović, Gordana D., Kazazić, Saša, Vrvić, Miroslav M., Brčeski, Ilija, Beškoski, Vladimir, Lončarević, Branka D., Gopčević, Kristina, Karadžić, Ivanka M., "Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h" in RSC Advances (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3179 .

TY  - THES
AU  - Rikalović, Milena G.
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=250
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:5525/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=43574799
UR  - http://nardus.mpn.gov.rs/123456789/3461
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2605
AB  - Cilj ove studije je bio da se ispita proizvodnja ramnolipida dobijenih pomoćupet sojeva Pseudomonas aeruginosa, izolovanih iz sredina bogatihugljovodonicima (zemljišta zagađenog naftom i naftnim derivatima iindustrijskog otpada – mineralnog ulja za sečenje metala). Prvi deo istraživanjaje bila optimizacija proizvodnje ramnolipida pomoću soja P. aeruginosa NCAIM(P) B 001380, producenta nekoliko mikrobioloških metabolita koji imajupotencijal za industrijsku primenu. Podloge sa različitim sastavom (izvorugljenika i azota) su testirane kako bi se poboljšala produkciju ramnolipida.Phosphate-limited proteose peptone-ammonium salt (PPAS) podloga sasuncokretovim uljem, kao izvorom ugljenika, i amonijum hloridom i peptonom,kao izvorom azota, značajno je povećala proizvodnju ramnolipida od 0,15 naosnovnoj PPAS (C/N odnos 4,0), na 3,00 g/l, na optimizovanoj PPAS podlozi(C/N odnos 7,7). U nastavku je primenjena statistička analiza Response SurfaceMethodology za testiranje efekata tri faktora: temperature, koncentracije izvoraugljenika i azota (v/v) u optimizovanoj PPAS podlozi, na proizvodnjuramnolipida. Utvrđeno je da ekološki izolati P. aeruginosa imaju značajanpotencijal za poboljšavanje produkcije ramnolipida na različitim tipovimasupstrata, uključujući ekonomične, obnovljive izvore kao što su suncokretovoulje iz friteze i otpadne frakcije tokom rafinisanja suncokretovog ulja. MSanalize su pokazale da su izolovani ramnolipidni biosurfaktanti smeše monoramno-mono-lipidnih, mono-ramno-di-lipidnih- i di-ramno-di-lipidnihjedinjenja. “High througout skrining“ ramnolipidnih smeša dobijenih pomoćurazličitih sojeva P. aeruginosa (NCAIM (P) B 001380, D1, D2, D3, 67) ireferentnog kliničkog soja ATCC 27853 na optimizovanoj podlozi urađen jepomoću MALDI-TOF analize. Razlike između ramnolipidnih profila koje suspecifične za soj su ukazale na mogućnost da se definiše ramnolipid“fingerprint” analiziranih izolata P. aeruginosa. Detaljna komparativna analizaramnolipida je urađena kako bi se video efekat uslova kultivacije na soj - specifični “fingerprint”ramnolipida. Ramnolipidi su praćeni pomoću HPLC-ESIMS, što je omogućilo brzu i pouzdanu identifikacije i kvantifikaciju prisutnihstruktura. Najveća koncentracija ukupnih ramnolipida od 3,33 g/l dobijena je za soj P. aeruginosa 67, koji je izolovan iz zemljišta zagađenog naftom i sojeve D1(1,73 g/l) i D2 (1,70 g/l), iz prirodnog mikrobnog konzorcijumima poreklom izzemljišta zagađenog mazutom, gajenih na suncokretovom ulju, kao izvoruugljenika...
AB  - The aim of present study was to investigate the production of rhamnolipidbiosurfactant produced by five strains of Pseudomonas aeruginosa originatedfrom hydrocarbon rich environments (soil polluted with petroleum andpetroleum derivates and industrial waste - mineral mettal cutting oil). The firstpart of the study was optimization of production of rhamnolipids by strain P.aeruginosa NCAIM (P) B 001380, a producer of several microbial methaboliteswith potential industrial application. With regard to carbon and nitrogen sourceseveral media were tested to enhance production of rhamnolipids. Phosphatelimitedproteose peptone-ammonium salt (PPAS) medium supplemented withsunflower oil as a source of carbon and mineral ammonium chloride andpeptone as a nitrogen source greatly improved rhamnolipid production, from0.15 on basic PPAS (C/N ratio 4.0), to 3.00 g/l, on optimized PPAS medium(C/N ratio 7.7). Further, the effect of three factors: temperature, concentrationof carbon and nitrogen source in optimized PPAS medium on rhamnolipidproduction was analyzed by Response Surface Methodology. It was found thatthe environmental isolates of P. aeruginosa had a significant potential forimproved production of rhamnolipids on different types of substrates,including cheap, renewable sources like sunflower oil from deep fryer andsunflower oil mill effluent. MS analysis indicated that rhamnolipid preparationswere mixtures of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic- and dirhamno-di-lipidic congeners. High througout screening of rhamnolipidmixtures produced by different environmental P. aeruginosa strains (NCAIM (P)B 001380, D1, D2, D3, 67) and referent clinical strain ATCC 27853 on optimisedmedium was performed by MALDI-TOF analysis. Observed differencesbetween rhamnolipid profiles indicated a possibility to define rhamnolipidfingerprint of analyzed P. aeruginosa strains. Detailed comparative analysis ofrhamnolipids from environmental isolates of P. aeruginosa was undertaken toevaluate strain specific rhamnolipid fingerprints obtained on different growth conditions...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Ispitivanje ramnolipida dobijenih pomoću sojeva Pseudomonas aeruginosa izolovanih iz sredina zagađenih naftom i naftnim derivatima
T1  - Study of rhamnolipid biosurfactant from strains of Pdeudomonas aeruginosa originated from environments contaminated with petroleum and petroleum derivates
UR  - https://hdl.handle.net/21.15107/rcub_nardus_3461
ER  - 

@phdthesis{
author = "Rikalović, Milena G.",
year = "2013",
abstract = "Cilj ove studije je bio da se ispita proizvodnja ramnolipida dobijenih pomoćupet sojeva Pseudomonas aeruginosa, izolovanih iz sredina bogatihugljovodonicima (zemljišta zagađenog naftom i naftnim derivatima iindustrijskog otpada – mineralnog ulja za sečenje metala). Prvi deo istraživanjaje bila optimizacija proizvodnje ramnolipida pomoću soja P. aeruginosa NCAIM(P) B 001380, producenta nekoliko mikrobioloških metabolita koji imajupotencijal za industrijsku primenu. Podloge sa različitim sastavom (izvorugljenika i azota) su testirane kako bi se poboljšala produkciju ramnolipida.Phosphate-limited proteose peptone-ammonium salt (PPAS) podloga sasuncokretovim uljem, kao izvorom ugljenika, i amonijum hloridom i peptonom,kao izvorom azota, značajno je povećala proizvodnju ramnolipida od 0,15 naosnovnoj PPAS (C/N odnos 4,0), na 3,00 g/l, na optimizovanoj PPAS podlozi(C/N odnos 7,7). U nastavku je primenjena statistička analiza Response SurfaceMethodology za testiranje efekata tri faktora: temperature, koncentracije izvoraugljenika i azota (v/v) u optimizovanoj PPAS podlozi, na proizvodnjuramnolipida. Utvrđeno je da ekološki izolati P. aeruginosa imaju značajanpotencijal za poboljšavanje produkcije ramnolipida na različitim tipovimasupstrata, uključujući ekonomične, obnovljive izvore kao što su suncokretovoulje iz friteze i otpadne frakcije tokom rafinisanja suncokretovog ulja. MSanalize su pokazale da su izolovani ramnolipidni biosurfaktanti smeše monoramno-mono-lipidnih, mono-ramno-di-lipidnih- i di-ramno-di-lipidnihjedinjenja. “High througout skrining“ ramnolipidnih smeša dobijenih pomoćurazličitih sojeva P. aeruginosa (NCAIM (P) B 001380, D1, D2, D3, 67) ireferentnog kliničkog soja ATCC 27853 na optimizovanoj podlozi urađen jepomoću MALDI-TOF analize. Razlike između ramnolipidnih profila koje suspecifične za soj su ukazale na mogućnost da se definiše ramnolipid“fingerprint” analiziranih izolata P. aeruginosa. Detaljna komparativna analizaramnolipida je urađena kako bi se video efekat uslova kultivacije na soj - specifični “fingerprint”ramnolipida. Ramnolipidi su praćeni pomoću HPLC-ESIMS, što je omogućilo brzu i pouzdanu identifikacije i kvantifikaciju prisutnihstruktura. Najveća koncentracija ukupnih ramnolipida od 3,33 g/l dobijena je za soj P. aeruginosa 67, koji je izolovan iz zemljišta zagađenog naftom i sojeve D1(1,73 g/l) i D2 (1,70 g/l), iz prirodnog mikrobnog konzorcijumima poreklom izzemljišta zagađenog mazutom, gajenih na suncokretovom ulju, kao izvoruugljenika..., The aim of present study was to investigate the production of rhamnolipidbiosurfactant produced by five strains of Pseudomonas aeruginosa originatedfrom hydrocarbon rich environments (soil polluted with petroleum andpetroleum derivates and industrial waste - mineral mettal cutting oil). The firstpart of the study was optimization of production of rhamnolipids by strain P.aeruginosa NCAIM (P) B 001380, a producer of several microbial methaboliteswith potential industrial application. With regard to carbon and nitrogen sourceseveral media were tested to enhance production of rhamnolipids. Phosphatelimitedproteose peptone-ammonium salt (PPAS) medium supplemented withsunflower oil as a source of carbon and mineral ammonium chloride andpeptone as a nitrogen source greatly improved rhamnolipid production, from0.15 on basic PPAS (C/N ratio 4.0), to 3.00 g/l, on optimized PPAS medium(C/N ratio 7.7). Further, the effect of three factors: temperature, concentrationof carbon and nitrogen source in optimized PPAS medium on rhamnolipidproduction was analyzed by Response Surface Methodology. It was found thatthe environmental isolates of P. aeruginosa had a significant potential forimproved production of rhamnolipids on different types of substrates,including cheap, renewable sources like sunflower oil from deep fryer andsunflower oil mill effluent. MS analysis indicated that rhamnolipid preparationswere mixtures of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic- and dirhamno-di-lipidic congeners. High througout screening of rhamnolipidmixtures produced by different environmental P. aeruginosa strains (NCAIM (P)B 001380, D1, D2, D3, 67) and referent clinical strain ATCC 27853 on optimisedmedium was performed by MALDI-TOF analysis. Observed differencesbetween rhamnolipid profiles indicated a possibility to define rhamnolipidfingerprint of analyzed P. aeruginosa strains. Detailed comparative analysis ofrhamnolipids from environmental isolates of P. aeruginosa was undertaken toevaluate strain specific rhamnolipid fingerprints obtained on different growth conditions...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Ispitivanje ramnolipida dobijenih pomoću sojeva Pseudomonas aeruginosa izolovanih iz sredina zagađenih naftom i naftnim derivatima, Study of rhamnolipid biosurfactant from strains of Pdeudomonas aeruginosa originated from environments contaminated with petroleum and petroleum derivates",
url = "https://hdl.handle.net/21.15107/rcub_nardus_3461"
}

Rikalović, M. G.. (2013). Ispitivanje ramnolipida dobijenih pomoću sojeva Pseudomonas aeruginosa izolovanih iz sredina zagađenih naftom i naftnim derivatima. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_3461

Rikalović MG. Ispitivanje ramnolipida dobijenih pomoću sojeva Pseudomonas aeruginosa izolovanih iz sredina zagađenih naftom i naftnim derivatima. in Универзитет у Београду. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_3461 .

Rikalović, Milena G., "Ispitivanje ramnolipida dobijenih pomoću sojeva Pseudomonas aeruginosa izolovanih iz sredina zagađenih naftom i naftnim derivatima" in Универзитет у Београду (2013),
https://hdl.handle.net/21.15107/rcub_nardus_3461 .