TY  - JOUR
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag V.
AU  - Vesović, Nikola
AU  - Todosijević, Marina
AU  - Pavićević, Miloš
AU  - Radović, Dejan
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5891
AB  - Pygidial glands are of great importance to ground beetles for defense against predators, especially for the species that live in subterranean habitats. The purpose of our study is to better understand the chemistry of the pygidial gland secretions of subterranean ground beetles, as well as the function and structure of the glands. We studied both the chemical composition of the pygidial gland secretion and morphology of the glands in adults of the troglophilic ground beetle species Laemostenus (Antisphodrus) cavicola (Schaum, 1858). The chemical composition of its defensive secretion was revealed using gas chromatography-mass spectrometry (GC-MS), while pygidial gland morphology of the beetle was investigated using bright-field microcopy. In total, seven chemical compounds were detected in the secretion mixture. Formic acid was the most dominant compound, followed by dodecyl acetate and undecane. Other chemicals were present in minor amounts. The morphological structure of the pygidial glands of L. (A.) cavicola was compared with the structure of the glands of the related congeneric troglophilic species Laemostenus (Pristonychus) punctatus (Dejean, 1828). Summary data on the semiochemicals that have been recorded so far in subterranean ground beetle species are presented, and the differences in the chemical composition of the secretions between and among troglobitic and troglophilic species are discussed. So far, forty-four compounds have been detected in four subterranean ground beetle species (two troglobites belonging to the tribe Trechini and two troglophiles belonging to the tribe Sphodrini). The results of this study indicate the great diversity of chemicals in the pygidial gland secretions of subterranean ground beetles. © 2023 by the authors.
PB  - MDPI
T2  - Diversity
T1  - On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)
VL  - 15
IS  - 2
DO  - 10.3390/d15020136
ER  - 

@article{
author = "Vranić, Sofija and Vujisić, Ljubodrag V. and Vesović, Nikola and Todosijević, Marina and Pavićević, Miloš and Radović, Dejan and Ćurčić, Srećko",
year = "2023",
abstract = "Pygidial glands are of great importance to ground beetles for defense against predators, especially for the species that live in subterranean habitats. The purpose of our study is to better understand the chemistry of the pygidial gland secretions of subterranean ground beetles, as well as the function and structure of the glands. We studied both the chemical composition of the pygidial gland secretion and morphology of the glands in adults of the troglophilic ground beetle species Laemostenus (Antisphodrus) cavicola (Schaum, 1858). The chemical composition of its defensive secretion was revealed using gas chromatography-mass spectrometry (GC-MS), while pygidial gland morphology of the beetle was investigated using bright-field microcopy. In total, seven chemical compounds were detected in the secretion mixture. Formic acid was the most dominant compound, followed by dodecyl acetate and undecane. Other chemicals were present in minor amounts. The morphological structure of the pygidial glands of L. (A.) cavicola was compared with the structure of the glands of the related congeneric troglophilic species Laemostenus (Pristonychus) punctatus (Dejean, 1828). Summary data on the semiochemicals that have been recorded so far in subterranean ground beetle species are presented, and the differences in the chemical composition of the secretions between and among troglobitic and troglophilic species are discussed. So far, forty-four compounds have been detected in four subterranean ground beetle species (two troglobites belonging to the tribe Trechini and two troglophiles belonging to the tribe Sphodrini). The results of this study indicate the great diversity of chemicals in the pygidial gland secretions of subterranean ground beetles. © 2023 by the authors.",
publisher = "MDPI",
journal = "Diversity",
title = "On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)",
volume = "15",
number = "2",
doi = "10.3390/d15020136"
}

Vranić, S., Vujisić, L. V., Vesović, N., Todosijević, M., Pavićević, M., Radović, D.,& Ćurčić, S.. (2023). On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae). in Diversity
MDPI., 15(2).
https://doi.org/10.3390/d15020136

Vranić S, Vujisić LV, Vesović N, Todosijević M, Pavićević M, Radović D, Ćurčić S. On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae). in Diversity. 2023;15(2).
doi:10.3390/d15020136 .

Vranić, Sofija, Vujisić, Ljubodrag V., Vesović, Nikola, Todosijević, Marina, Pavićević, Miloš, Radović, Dejan, Ćurčić, Srećko, "On the Diversity of Semiochemicals of the Pygidial Gland Secretions of Subterranean Ground Beetles (Coleoptera: Carabidae)" in Diversity, 15, no. 2 (2023),
https://doi.org/10.3390/d15020136 . .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Nenadić, Marija
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag V.
AU  - Milinčić, Katarina M.
AU  - Todosijević, Marina
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Ćurčić, Nina B.
AU  - Stevanović, Nataša
AU  - Mihajlović, Ljiljana
AU  - Vukoičić, Danijela Ž.
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6189
AB  - The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.
PB  - Frontiers Media
T2  - Frontiers in Ecology and Evolution
T1  - The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)
VL  - 11
DO  - 10.3389/fevo.2023.1120006
ER  - 

@article{
author = "Vesović, Nikola and Nenadić, Marija and Vranić, Sofija and Vujisić, Ljubodrag V. and Milinčić, Katarina M. and Todosijević, Marina and Dimkić, Ivica and Janakiev, Tamara and Ćurčić, Nina B. and Stevanović, Nataša and Mihajlović, Ljiljana and Vukoičić, Danijela Ž. and Ćurčić, Srećko",
year = "2023",
abstract = "The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.",
publisher = "Frontiers Media",
journal = "Frontiers in Ecology and Evolution",
title = "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)",
volume = "11",
doi = "10.3389/fevo.2023.1120006"
}

Vesović, N., Nenadić, M., Vranić, S., Vujisić, L. V., Milinčić, K. M., Todosijević, M., Dimkić, I., Janakiev, T., Ćurčić, N. B., Stevanović, N., Mihajlović, L., Vukoičić, D. Ž.,& Ćurčić, S.. (2023). The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution
Frontiers Media., 11.
https://doi.org/10.3389/fevo.2023.1120006

Vesović N, Nenadić M, Vranić S, Vujisić LV, Milinčić KM, Todosijević M, Dimkić I, Janakiev T, Ćurčić NB, Stevanović N, Mihajlović L, Vukoičić DŽ, Ćurčić S. The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae). in Frontiers in Ecology and Evolution. 2023;11.
doi:10.3389/fevo.2023.1120006 .

Vesović, Nikola, Nenadić, Marija, Vranić, Sofija, Vujisić, Ljubodrag V., Milinčić, Katarina M., Todosijević, Marina, Dimkić, Ivica, Janakiev, Tamara, Ćurčić, Nina B., Stevanović, Nataša, Mihajlović, Ljiljana, Vukoičić, Danijela Ž., Ćurčić, Srećko, "The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)" in Frontiers in Ecology and Evolution, 11 (2023),
https://doi.org/10.3389/fevo.2023.1120006 . .

TY  - JOUR
AU  - Vranić, Sofija
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag V.
AU  - Pavlović, Danica
AU  - Pantelić, Dejan
AU  - Todosijević, Marina
AU  - Ćurčić, Srećko
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0944200621000568
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4606
AB  - Morphology of the pygidial glands and chemical compositions of their secretion were analysed in the adults of three selected ground beetle taxa. Secretions of pygidial glands of Cychrus (Cychrus) semigranosus, Patrobus atrorufus and Pterostichus (Platysma) niger were chemically tested. Additionally, pygidial glands of the latter two species were investigated using bright-field microscopy and nonlinear microscopy and morphological features of the glands were described in detail. Both C. (C.) semigranosus and P. atrorufus were studied for the first time in terms of chemical ecology, while the latter species was analysed for the first time in terms of pygidial gland morphology. Altogether, eight compounds were detected in the dichloromethane extracts of the pygidial gland secretions of the three ground beetle taxa analysed. The simplest secretion mixtures were present in C. (C.) semigranosus and P. atrorufus (with two compounds each), while the extract of P. (P.) niger contained five compounds. The presence of 1-tetradecanol in the secretion of P. (P.) niger represents the first finding of this compound from the pygidial gland secretion extracts of ground beetles.
PB  - Elsevier
T2  - Zoology
T1  - Pygidial glands of three ground beetle taxa (Insecta, Coleoptera, Carabidae): a study on their morphology and chemical composition of their secretions
VL  - 148
SP  - 125948
DO  - 10.1016/j.zool.2021.125948
ER  - 

@article{
author = "Vranić, Sofija and Vesović, Nikola and Vujisić, Ljubodrag V. and Pavlović, Danica and Pantelić, Dejan and Todosijević, Marina and Ćurčić, Srećko",
year = "2021",
abstract = "Morphology of the pygidial glands and chemical compositions of their secretion were analysed in the adults of three selected ground beetle taxa. Secretions of pygidial glands of Cychrus (Cychrus) semigranosus, Patrobus atrorufus and Pterostichus (Platysma) niger were chemically tested. Additionally, pygidial glands of the latter two species were investigated using bright-field microscopy and nonlinear microscopy and morphological features of the glands were described in detail. Both C. (C.) semigranosus and P. atrorufus were studied for the first time in terms of chemical ecology, while the latter species was analysed for the first time in terms of pygidial gland morphology. Altogether, eight compounds were detected in the dichloromethane extracts of the pygidial gland secretions of the three ground beetle taxa analysed. The simplest secretion mixtures were present in C. (C.) semigranosus and P. atrorufus (with two compounds each), while the extract of P. (P.) niger contained five compounds. The presence of 1-tetradecanol in the secretion of P. (P.) niger represents the first finding of this compound from the pygidial gland secretion extracts of ground beetles.",
publisher = "Elsevier",
journal = "Zoology",
title = "Pygidial glands of three ground beetle taxa (Insecta, Coleoptera, Carabidae): a study on their morphology and chemical composition of their secretions",
volume = "148",
pages = "125948",
doi = "10.1016/j.zool.2021.125948"
}

Vranić, S., Vesović, N., Vujisić, L. V., Pavlović, D., Pantelić, D., Todosijević, M.,& Ćurčić, S.. (2021). Pygidial glands of three ground beetle taxa (Insecta, Coleoptera, Carabidae): a study on their morphology and chemical composition of their secretions. in Zoology
Elsevier., 148, 125948.
https://doi.org/10.1016/j.zool.2021.125948

Vranić S, Vesović N, Vujisić LV, Pavlović D, Pantelić D, Todosijević M, Ćurčić S. Pygidial glands of three ground beetle taxa (Insecta, Coleoptera, Carabidae): a study on their morphology and chemical composition of their secretions. in Zoology. 2021;148:125948.
doi:10.1016/j.zool.2021.125948 .

Vranić, Sofija, Vesović, Nikola, Vujisić, Ljubodrag V., Pavlović, Danica, Pantelić, Dejan, Todosijević, Marina, Ćurčić, Srećko, "Pygidial glands of three ground beetle taxa (Insecta, Coleoptera, Carabidae): a study on their morphology and chemical composition of their secretions" in Zoology, 148 (2021):125948,
https://doi.org/10.1016/j.zool.2021.125948 . .

TY  - JOUR
AU  - Vranić, Sofija
AU  - Ćurčić, Srećko
AU  - Vesović, Nikola
AU  - Mandić, Boris
AU  - Pantelić, Dejan
AU  - Vasović, Marija
AU  - Lazović, Vladimir
AU  - Zhang, Wang
AU  - Vujisić, Ljubodrag V.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4240
AB  - Morphology of the pygidial glands and chemical composition of their secretions in adults of four ground beetle representatives of the Pterostichini tribe (Coleoptera: Carabidae) were analysed. Molops (Stenochoromus) montenegrinus, Pterostichus (Cophosus) cylindricus, P. (Feronidius) melas and P. (Pseudomaseus) nigrita were chemically tested, while the latter three species were morphologically investigated. Pterostichus (C.) cylindricus, P. (P.) nigrita and M. (S.) montenegrinus were chemically studied for the first time. Altogether, 23 chemical compounds were isolated using gas chromatography-mass spectrometry (GC-MS), of which some are new for Pterostichini or even Carabidae. Methacrylic acid was present in all species analysed. It was predominant in the secretion extract of P. (C.) cylindricus and P. (F.) melas. Isobutyric and 2-methylbutyric acids were the major components in the secretion of M. (S.) montenegrinus. Undecane, methacrylic and tiglic acids were the main components in the secretion of P. (P.) nigrita. The simplest chemical mixture was found in P. (C.) cylindricus (two compounds), while the most complex one was detected in P. (P.) nigrita (15 compounds). No significant differences in the chemical composition of the pygidial gland secretions were evidenced in P. (C.) cylindricus sampled from the same area and in the same season in two different years. Morphology of the pygidial glands of the studied species was analysed for the first time. Morphological features of the pygidial glands were observed using bright-field microscopy and nonlinear microscopy and described in details.
PB  - Elsevier
T2  - Zoology
T1  - Chemistry and morphology of the pygidial glands in four Pterostichini ground beetle taxa (Coleoptera: Carabidae: Pterostichinae)
VL  - 142
SP  - 125772
DO  - 10.1016/j.zool.2020.125772
ER  - 

@article{
author = "Vranić, Sofija and Ćurčić, Srećko and Vesović, Nikola and Mandić, Boris and Pantelić, Dejan and Vasović, Marija and Lazović, Vladimir and Zhang, Wang and Vujisić, Ljubodrag V.",
year = "2020",
abstract = "Morphology of the pygidial glands and chemical composition of their secretions in adults of four ground beetle representatives of the Pterostichini tribe (Coleoptera: Carabidae) were analysed. Molops (Stenochoromus) montenegrinus, Pterostichus (Cophosus) cylindricus, P. (Feronidius) melas and P. (Pseudomaseus) nigrita were chemically tested, while the latter three species were morphologically investigated. Pterostichus (C.) cylindricus, P. (P.) nigrita and M. (S.) montenegrinus were chemically studied for the first time. Altogether, 23 chemical compounds were isolated using gas chromatography-mass spectrometry (GC-MS), of which some are new for Pterostichini or even Carabidae. Methacrylic acid was present in all species analysed. It was predominant in the secretion extract of P. (C.) cylindricus and P. (F.) melas. Isobutyric and 2-methylbutyric acids were the major components in the secretion of M. (S.) montenegrinus. Undecane, methacrylic and tiglic acids were the main components in the secretion of P. (P.) nigrita. The simplest chemical mixture was found in P. (C.) cylindricus (two compounds), while the most complex one was detected in P. (P.) nigrita (15 compounds). No significant differences in the chemical composition of the pygidial gland secretions were evidenced in P. (C.) cylindricus sampled from the same area and in the same season in two different years. Morphology of the pygidial glands of the studied species was analysed for the first time. Morphological features of the pygidial glands were observed using bright-field microscopy and nonlinear microscopy and described in details.",
publisher = "Elsevier",
journal = "Zoology",
title = "Chemistry and morphology of the pygidial glands in four Pterostichini ground beetle taxa (Coleoptera: Carabidae: Pterostichinae)",
volume = "142",
pages = "125772",
doi = "10.1016/j.zool.2020.125772"
}

Vranić, S., Ćurčić, S., Vesović, N., Mandić, B., Pantelić, D., Vasović, M., Lazović, V., Zhang, W.,& Vujisić, L. V.. (2020). Chemistry and morphology of the pygidial glands in four Pterostichini ground beetle taxa (Coleoptera: Carabidae: Pterostichinae). in Zoology
Elsevier., 142, 125772.
https://doi.org/10.1016/j.zool.2020.125772

Vranić S, Ćurčić S, Vesović N, Mandić B, Pantelić D, Vasović M, Lazović V, Zhang W, Vujisić LV. Chemistry and morphology of the pygidial glands in four Pterostichini ground beetle taxa (Coleoptera: Carabidae: Pterostichinae). in Zoology. 2020;142:125772.
doi:10.1016/j.zool.2020.125772 .

Vranić, Sofija, Ćurčić, Srećko, Vesović, Nikola, Mandić, Boris, Pantelić, Dejan, Vasović, Marija, Lazović, Vladimir, Zhang, Wang, Vujisić, Ljubodrag V., "Chemistry and morphology of the pygidial glands in four Pterostichini ground beetle taxa (Coleoptera: Carabidae: Pterostichinae)" in Zoology, 142 (2020):125772,
https://doi.org/10.1016/j.zool.2020.125772 . .