TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Ilić, Marija D.
AU  - Matić, Ivana Z.
AU  - Jovanović, Živko S.
AU  - Čupić, Tihomir
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2291
AB  - To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer. An International Journal
T1  - Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities
VL  - 68
IS  - 6
SP  - 988
EP  - 1000
DO  - 10.1080/01635581.2016.1190019
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj.",
year = "2016",
abstract = "To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer. An International Journal",
title = "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities",
volume = "68",
number = "6",
pages = "988-1000",
doi = "10.1080/01635581.2016.1190019"
}

Stanisavljević, N. S., Ilić, M. D., Matić, I. Z., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M.,& Tešić, Ž. Lj.. (2016). Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer. An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 68(6), 988-1000.
https://doi.org/10.1080/01635581.2016.1190019

Stanisavljević NS, Ilić MD, Matić IZ, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL. Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer. An International Journal. 2016;68(6):988-1000.
doi:10.1080/01635581.2016.1190019 .

Stanisavljević, Nemanja S., Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities" in Nutrition and Cancer. An International Journal, 68, no. 6 (2016):988-1000,
https://doi.org/10.1080/01635581.2016.1190019 . .

TY  - DATA
AU  - Stanisavljević, Nemanja S.
AU  - Ilić, Marija D.
AU  - Matić, Ivana Z.
AU  - Jovanović, Živko S.
AU  - Čupić, Tihomir
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3534
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer. An International Journal
T1  - Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3534
ER  - 

@misc{
author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj.",
year = "2016",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer. An International Journal",
title = "Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3534"
}

Stanisavljević, N. S., Ilić, M. D., Matić, I. Z., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M.,& Tešić, Ž. Lj.. (2016). Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.. in Nutrition and Cancer. An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon..
https://hdl.handle.net/21.15107/rcub_cherry_3534

Stanisavljević NS, Ilić MD, Matić IZ, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL. Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019.. in Nutrition and Cancer. An International Journal. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3534 .

Stanisavljević, Nemanja S., Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., "Supplementary material for the article: Stanisavljević, N. S.; Ilić, M. D.; Matić, I. Z.; Jovanović, Ž. S.; Čupić, T.; Dabić, D. Č.;  Natić, M. M.; Tešić, Ž. L. Identification of Phenolic Compounds from Seed Coats of  Differently Colored European Varieties of Pea (Pisum Sativum L.) and Characterization of  Their Antioxidant and In Vitro Anticancer Activities. Nutrition and Cancer 2016, 68 (6),  988–1000. https://doi.org/10.1080/01635581.2016.1190019." in Nutrition and Cancer. An International Journal (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3534 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Ilić, Marija D.
AU  - Jovanović, Živko S.
AU  - Čupić, Tihomir
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
AU  - Radovic, Svetlana S.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2013
AB  - The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity
VL  - 67
IS  - 3
SP  - 829
EP  - 840
DO  - 10.2298/ABS141204042S
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Ilić, Marija D. and Jovanović, Živko S. and Čupić, Tihomir and Dabić Zagorac, Dragana and Natić, Maja and Tešić, Živoslav Lj. and Radovic, Svetlana S.",
year = "2015",
abstract = "The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity",
volume = "67",
number = "3",
pages = "829-840",
doi = "10.2298/ABS141204042S"
}

Stanisavljević, N. S., Ilić, M. D., Jovanović, Ž. S., Čupić, T., Dabić Zagorac, D., Natić, M., Tešić, Ž. Lj.,& Radovic, S. S.. (2015). Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 67(3), 829-840.
https://doi.org/10.2298/ABS141204042S

Stanisavljević NS, Ilić MD, Jovanović ŽS, Čupić T, Dabić Zagorac D, Natić M, Tešić ŽL, Radovic SS. Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity. in Archives of biological sciences. 2015;67(3):829-840.
doi:10.2298/ABS141204042S .

Stanisavljević, Nemanja S., Ilić, Marija D., Jovanović, Živko S., Čupić, Tihomir, Dabić Zagorac, Dragana, Natić, Maja, Tešić, Živoslav Lj., Radovic, Svetlana S., "Identification of Seed Coat Phenolic Compounds from  Differently Colored Pea Varieties and Characterization of Their Antioxidant Activity" in Archives of biological sciences, 67, no. 3 (2015):829-840,
https://doi.org/10.2298/ABS141204042S . .