TY  - JOUR
AU  - Mrkalić, Emina
AU  - Šmit, Biljana
AU  - Matić, Sanja
AU  - Jelić, Ratomir
AU  - Ćendić Serafinović, Marina
AU  - Gligorijević, Nevenka
AU  - Čavić, Milena
AU  - Aranđelović, Sandra
AU  - Grgurić-Šipka, Sanja
AU  - Soldatović, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5880
AB  - The four novel complexes [{cis-PtCl(NH3)2(μ-4,4′ -bipyridyl)ZnCl(terpy)}](ClO4)2 (C1), [{trans-PtCl(NH3)2(μ-
4,4′ -bipyridyl)ZnCl(terpy)}](ClO4)2 (C2), [{cis-PtCl(NH3)2(μ-pyrazine)ZnCl(terpy)}](ClO4)2 (C3) and [{trans-
PtCl(NH3)2(μ-pyrazine)ZnCl(terpy)}](ClO4)2 (C4) (where terpy = 2,2′ :6′ ,2′ ′ -terpyridine) were synthesized and
characterized. Acid–base titrations and concentration dependent kinetic measurements for the reactions with
biologically relevant ligands such as guanosine-5′ -monophosphate (5′ -GMP), inosine-5′ -monophosphate (5′ -IMP)
and glutathione (GSH), were studied at pH 7.4 and 37 ◦C. The binding of the heterometallic bridged cis- or trans-
Pt(II)-Zn(II) complexes to calf thymus DNA (CT-DNA) was studied by UV absorption and fluorescence emission
spectroscopy and molecular docking. The results indicated that the complexes bind strongly to DNA, through
groove binding, hydrogen bonds, and hydrophobic or electrostatic interaction. The possible in vitro DNA protective
effect of cis- and trans-Pt-L-Zn complexes has shown that C3 had significant dose-dependent DNA-protective
effect and the same ability to inhibit peroxyl as well as hydroxyl radicals. Antiproliferative effect of the
complexes, mRNA expression of apoptosis and repair-related genes after treatment in cancer cells indicated that
newly synthesized C2 exhibited highly selective cytotoxicity toward colon carcinoma HCT116 cells. Only
treatment with trans analog C2 induced effect similar to the typical DNA damaging agent such as cisplatin,
characterized by p53 mediated cell response, cell cycle arrest and certain induction of apoptotic related genes.
Both cis- and trans-isomers C1 and C2 showed potency to elicit expression of PARP1 mRNA and in vitro DNA
binding.
PB  - Elsevier
T2  - J. Inorg. Biochem.
T1  - Exploring heterometallic bridged Pt(II)-Zn(II) complexes as potential antitumor agent
VL  - 240
DO  - https://doi.org/10.1016/j.jinorgbio.2022.112100
ER  - 

@article{
author = "Mrkalić, Emina and Šmit, Biljana and Matić, Sanja and Jelić, Ratomir and Ćendić Serafinović, Marina and Gligorijević, Nevenka and Čavić, Milena and Aranđelović, Sandra and Grgurić-Šipka, Sanja and Soldatović, Tanja",
year = "2023",
abstract = "The four novel complexes [{cis-PtCl(NH3)2(μ-4,4′ -bipyridyl)ZnCl(terpy)}](ClO4)2 (C1), [{trans-PtCl(NH3)2(μ-
4,4′ -bipyridyl)ZnCl(terpy)}](ClO4)2 (C2), [{cis-PtCl(NH3)2(μ-pyrazine)ZnCl(terpy)}](ClO4)2 (C3) and [{trans-
PtCl(NH3)2(μ-pyrazine)ZnCl(terpy)}](ClO4)2 (C4) (where terpy = 2,2′ :6′ ,2′ ′ -terpyridine) were synthesized and
characterized. Acid–base titrations and concentration dependent kinetic measurements for the reactions with
biologically relevant ligands such as guanosine-5′ -monophosphate (5′ -GMP), inosine-5′ -monophosphate (5′ -IMP)
and glutathione (GSH), were studied at pH 7.4 and 37 ◦C. The binding of the heterometallic bridged cis- or trans-
Pt(II)-Zn(II) complexes to calf thymus DNA (CT-DNA) was studied by UV absorption and fluorescence emission
spectroscopy and molecular docking. The results indicated that the complexes bind strongly to DNA, through
groove binding, hydrogen bonds, and hydrophobic or electrostatic interaction. The possible in vitro DNA protective
effect of cis- and trans-Pt-L-Zn complexes has shown that C3 had significant dose-dependent DNA-protective
effect and the same ability to inhibit peroxyl as well as hydroxyl radicals. Antiproliferative effect of the
complexes, mRNA expression of apoptosis and repair-related genes after treatment in cancer cells indicated that
newly synthesized C2 exhibited highly selective cytotoxicity toward colon carcinoma HCT116 cells. Only
treatment with trans analog C2 induced effect similar to the typical DNA damaging agent such as cisplatin,
characterized by p53 mediated cell response, cell cycle arrest and certain induction of apoptotic related genes.
Both cis- and trans-isomers C1 and C2 showed potency to elicit expression of PARP1 mRNA and in vitro DNA
binding.",
publisher = "Elsevier",
journal = "J. Inorg. Biochem.",
title = "Exploring heterometallic bridged Pt(II)-Zn(II) complexes as potential antitumor agent",
volume = "240",
doi = "https://doi.org/10.1016/j.jinorgbio.2022.112100"
}

Mrkalić, E., Šmit, B., Matić, S., Jelić, R., Ćendić Serafinović, M., Gligorijević, N., Čavić, M., Aranđelović, S., Grgurić-Šipka, S.,& Soldatović, T.. (2023). Exploring heterometallic bridged Pt(II)-Zn(II) complexes as potential antitumor agent. in J. Inorg. Biochem.
Elsevier., 240.
https://doi.org/https://doi.org/10.1016/j.jinorgbio.2022.112100

Mrkalić E, Šmit B, Matić S, Jelić R, Ćendić Serafinović M, Gligorijević N, Čavić M, Aranđelović S, Grgurić-Šipka S, Soldatović T. Exploring heterometallic bridged Pt(II)-Zn(II) complexes as potential antitumor agent. in J. Inorg. Biochem.. 2023;240.
doi:https://doi.org/10.1016/j.jinorgbio.2022.112100 .

Mrkalić, Emina, Šmit, Biljana, Matić, Sanja, Jelić, Ratomir, Ćendić Serafinović, Marina, Gligorijević, Nevenka, Čavić, Milena, Aranđelović, Sandra, Grgurić-Šipka, Sanja, Soldatović, Tanja, "Exploring heterometallic bridged Pt(II)-Zn(II) complexes as potential antitumor agent" in J. Inorg. Biochem., 240 (2023),
https://doi.org/https://doi.org/10.1016/j.jinorgbio.2022.112100 . .