TY  - JOUR
AU  - Thiesen, L. C. T.
AU  - Sugauara, E. Y. Y.
AU  - Tešević, Vele
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Goncalves, J. E.
AU  - Gazim, Z. C.
AU  - Linde, G. A.
AU  - Colauto, N. B.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2562
AB  - Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.
PB  - Funpec-Editora, Ribeirao Preto
T2  - Genetics and Molecular Research
T1  - Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
VL  - 16
IS  - 2
DO  - 10.4238/gmr16029548
ER  - 

@article{
author = "Thiesen, L. C. T. and Sugauara, E. Y. Y. and Tešević, Vele and Glamočlija, Jasmina and Soković, Marina and Goncalves, J. E. and Gazim, Z. C. and Linde, G. A. and Colauto, N. B.",
year = "2017",
abstract = "Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.",
publisher = "Funpec-Editora, Ribeirao Preto",
journal = "Genetics and Molecular Research",
title = "Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide",
volume = "16",
number = "2",
doi = "10.4238/gmr16029548"
}

Thiesen, L. C. T., Sugauara, E. Y. Y., Tešević, V., Glamočlija, J., Soković, M., Goncalves, J. E., Gazim, Z. C., Linde, G. A.,& Colauto, N. B.. (2017). Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. in Genetics and Molecular Research
Funpec-Editora, Ribeirao Preto., 16(2).
https://doi.org/10.4238/gmr16029548

Thiesen LCT, Sugauara EYY, Tešević V, Glamočlija J, Soković M, Goncalves JE, Gazim ZC, Linde GA, Colauto NB. Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. in Genetics and Molecular Research. 2017;16(2).
doi:10.4238/gmr16029548 .

Thiesen, L. C. T., Sugauara, E. Y. Y., Tešević, Vele, Glamočlija, Jasmina, Soković, Marina, Goncalves, J. E., Gazim, Z. C., Linde, G. A., Colauto, N. B., "Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide" in Genetics and Molecular Research, 16, no. 2 (2017),
https://doi.org/10.4238/gmr16029548 . .

TY  - JOUR
AU  - Linde, G. A.
AU  - Gazim, Z. C.
AU  - Cardoso, B. K.
AU  - Jorge, L. F.
AU  - Tešević, Vele
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Colauto, N. B.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2323
AB  - Parsley [Petroselinum crispum (Mill.) Fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. However, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. In addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. Also, microorganism resistance is still a challenge for health and food production. Based on the demand for natural products to control microorganisms, and the reevaluation of potential medicinal plants for controlling diseases, the objective of this study was to determine the chemical composition and antibacterial and antifungal activities of parsley essential oil against foodborne diseases and opportunistic pathogens. Seven bacteria and eight fungi were tested. The essential oil major compounds were apiol, myristicin, and beta-phellandrene. Parsley essential oil had bacteriostatic activity against all tested bacteria, mainly Staphylococcus aureus, Listeria monocytogenes, and Salmonella enterica, at similar or lower concentrations than at least one of the controls, and bactericidal activity against all tested bacteria, mainly S. aureus, at similar or lower concentrations than at least one of the controls. This essential oil also had fungistatic activity against all tested fungi, mainly, Penicillium ochrochloron and Trichoderma viride, at lower concentrations than the ketoconazole control and fungicidal activity against all tested fungi at higher concentrations than the controls. Parsley is used in cooking and medicine, and its essential oil is an effective antimicrobial agent.
PB  - Funpec-Editora, Ribeirao Preto
T2  - Genetics and Molecular Research
T1  - Antifungal and antibacterial activities of Petroselinum crispum essential oil
VL  - 15
IS  - 3
DO  - 10.4238/gmr.15038538
ER  - 

@article{
author = "Linde, G. A. and Gazim, Z. C. and Cardoso, B. K. and Jorge, L. F. and Tešević, Vele and Glamočlija, Jasmina and Soković, Marina and Colauto, N. B.",
year = "2016",
abstract = "Parsley [Petroselinum crispum (Mill.) Fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. However, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. In addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. Also, microorganism resistance is still a challenge for health and food production. Based on the demand for natural products to control microorganisms, and the reevaluation of potential medicinal plants for controlling diseases, the objective of this study was to determine the chemical composition and antibacterial and antifungal activities of parsley essential oil against foodborne diseases and opportunistic pathogens. Seven bacteria and eight fungi were tested. The essential oil major compounds were apiol, myristicin, and beta-phellandrene. Parsley essential oil had bacteriostatic activity against all tested bacteria, mainly Staphylococcus aureus, Listeria monocytogenes, and Salmonella enterica, at similar or lower concentrations than at least one of the controls, and bactericidal activity against all tested bacteria, mainly S. aureus, at similar or lower concentrations than at least one of the controls. This essential oil also had fungistatic activity against all tested fungi, mainly, Penicillium ochrochloron and Trichoderma viride, at lower concentrations than the ketoconazole control and fungicidal activity against all tested fungi at higher concentrations than the controls. Parsley is used in cooking and medicine, and its essential oil is an effective antimicrobial agent.",
publisher = "Funpec-Editora, Ribeirao Preto",
journal = "Genetics and Molecular Research",
title = "Antifungal and antibacterial activities of Petroselinum crispum essential oil",
volume = "15",
number = "3",
doi = "10.4238/gmr.15038538"
}

Linde, G. A., Gazim, Z. C., Cardoso, B. K., Jorge, L. F., Tešević, V., Glamočlija, J., Soković, M.,& Colauto, N. B.. (2016). Antifungal and antibacterial activities of Petroselinum crispum essential oil. in Genetics and Molecular Research
Funpec-Editora, Ribeirao Preto., 15(3).
https://doi.org/10.4238/gmr.15038538

Linde GA, Gazim ZC, Cardoso BK, Jorge LF, Tešević V, Glamočlija J, Soković M, Colauto NB. Antifungal and antibacterial activities of Petroselinum crispum essential oil. in Genetics and Molecular Research. 2016;15(3).
doi:10.4238/gmr.15038538 .

Linde, G. A., Gazim, Z. C., Cardoso, B. K., Jorge, L. F., Tešević, Vele, Glamočlija, Jasmina, Soković, Marina, Colauto, N. B., "Antifungal and antibacterial activities of Petroselinum crispum essential oil" in Genetics and Molecular Research, 15, no. 3 (2016),
https://doi.org/10.4238/gmr.15038538 . .