TY  - JOUR
AU  - Sentić, Milica
AU  - Milutinovic, Milena
AU  - Kanoufi, Frederic
AU  - Manojlović, Dragan D.
AU  - Arbault, Stephane
AU  - Šojić, Nešo
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1779
AB  - The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Science
T1  - Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays
VL  - 5
IS  - 6
SP  - 2568
EP  - 2572
DO  - 10.1039/c4sc00312h
ER  - 

@article{
author = "Sentić, Milica and Milutinovic, Milena and Kanoufi, Frederic and Manojlović, Dragan D. and Arbault, Stephane and Šojić, Nešo",
year = "2014",
abstract = "The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Science",
title = "Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays",
volume = "5",
number = "6",
pages = "2568-2572",
doi = "10.1039/c4sc00312h"
}

Sentić, M., Milutinovic, M., Kanoufi, F., Manojlović, D. D., Arbault, S.,& Šojić, N.. (2014). Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays. in Chemical Science
Royal Soc Chemistry, Cambridge., 5(6), 2568-2572.
https://doi.org/10.1039/c4sc00312h

Sentić M, Milutinovic M, Kanoufi F, Manojlović DD, Arbault S, Šojić N. Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays. in Chemical Science. 2014;5(6):2568-2572.
doi:10.1039/c4sc00312h .

Sentić, Milica, Milutinovic, Milena, Kanoufi, Frederic, Manojlović, Dragan D., Arbault, Stephane, Šojić, Nešo, "Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays" in Chemical Science, 5, no. 6 (2014):2568-2572,
https://doi.org/10.1039/c4sc00312h . .

TY  - JOUR
AU  - Milutinovic, Milena
AU  - Sallard, Sebastien
AU  - Manojlović, Dragan D.
AU  - Mano, Nicolas
AU  - Šojić, Nešo
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1203
AB  - In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Sa, Lausanne
T2  - Bioelectrochemistry
T1  - Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel
VL  - 82
IS  - 1
SP  - 63
EP  - 68
DO  - 10.1016/j.bioelechem.2011.05.005
ER  - 

@article{
author = "Milutinovic, Milena and Sallard, Sebastien and Manojlović, Dragan D. and Mano, Nicolas and Šojić, Nešo",
year = "2011",
abstract = "In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Bioelectrochemistry",
title = "Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel",
volume = "82",
number = "1",
pages = "63-68",
doi = "10.1016/j.bioelechem.2011.05.005"
}

Milutinovic, M., Sallard, S., Manojlović, D. D., Mano, N.,& Šojić, N.. (2011). Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel. in Bioelectrochemistry
Elsevier Science Sa, Lausanne., 82(1), 63-68.
https://doi.org/10.1016/j.bioelechem.2011.05.005

Milutinovic M, Sallard S, Manojlović DD, Mano N, Šojić N. Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel. in Bioelectrochemistry. 2011;82(1):63-68.
doi:10.1016/j.bioelechem.2011.05.005 .

Milutinovic, Milena, Sallard, Sebastien, Manojlović, Dragan D., Mano, Nicolas, Šojić, Nešo, "Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel" in Bioelectrochemistry, 82, no. 1 (2011):63-68,
https://doi.org/10.1016/j.bioelechem.2011.05.005 . .

TY  - JOUR
AU  - Milutinovic, Milena
AU  - Suraniti, Emmanuel
AU  - Studer, Vincent
AU  - Mano, Nicolas
AU  - Manojlović, Dragan D.
AU  - Šojić, Nešo
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1185
AB  - Photoinitiated polymerisation is efficiently and rapidly carried out to immobilise ultrathin electrochemiluminescent redox hydrogel films. Microscale patterns are fabricated on an electrode surface by a simple photolithographic procedure and revealed by ECL imaging.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Communications
T1  - Photopatterning of ultrathin electrochemiluminescent redox hydrogel films
VL  - 47
IS  - 32
SP  - 9125
EP  - 9127
DO  - 10.1039/c1cc12724a
ER  - 

@article{
author = "Milutinovic, Milena and Suraniti, Emmanuel and Studer, Vincent and Mano, Nicolas and Manojlović, Dragan D. and Šojić, Nešo",
year = "2011",
abstract = "Photoinitiated polymerisation is efficiently and rapidly carried out to immobilise ultrathin electrochemiluminescent redox hydrogel films. Microscale patterns are fabricated on an electrode surface by a simple photolithographic procedure and revealed by ECL imaging.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Communications",
title = "Photopatterning of ultrathin electrochemiluminescent redox hydrogel films",
volume = "47",
number = "32",
pages = "9125-9127",
doi = "10.1039/c1cc12724a"
}

Milutinovic, M., Suraniti, E., Studer, V., Mano, N., Manojlović, D. D.,& Šojić, N.. (2011). Photopatterning of ultrathin electrochemiluminescent redox hydrogel films. in Chemical Communications
Royal Soc Chemistry, Cambridge., 47(32), 9125-9127.
https://doi.org/10.1039/c1cc12724a

Milutinovic M, Suraniti E, Studer V, Mano N, Manojlović DD, Šojić N. Photopatterning of ultrathin electrochemiluminescent redox hydrogel films. in Chemical Communications. 2011;47(32):9125-9127.
doi:10.1039/c1cc12724a .

Milutinovic, Milena, Suraniti, Emmanuel, Studer, Vincent, Mano, Nicolas, Manojlović, Dragan D., Šojić, Nešo, "Photopatterning of ultrathin electrochemiluminescent redox hydrogel films" in Chemical Communications, 47, no. 32 (2011):9125-9127,
https://doi.org/10.1039/c1cc12724a . .

TY  - CONF
AU  - Milutinovic, Milena
AU  - Sallard, Sebastien
AU  - Manojlović, Dragan D.
AU  - Mano, Nicolas
AU  - Šojić, Nešo
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1074
PB  - John Wiley & Sons Ltd, Chichester
C3  - Luminescence
T1  - Electrogenerated chemiluminescence in an electrodeposited redox polymer
VL  - 25
IS  - 2
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1074
ER  - 

@conference{
author = "Milutinovic, Milena and Sallard, Sebastien and Manojlović, Dragan D. and Mano, Nicolas and Šojić, Nešo",
year = "2010",
publisher = "John Wiley & Sons Ltd, Chichester",
journal = "Luminescence",
title = "Electrogenerated chemiluminescence in an electrodeposited redox polymer",
volume = "25",
number = "2",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1074"
}

Milutinovic, M., Sallard, S., Manojlović, D. D., Mano, N.,& Šojić, N.. (2010). Electrogenerated chemiluminescence in an electrodeposited redox polymer. in Luminescence
John Wiley & Sons Ltd, Chichester., 25(2), 85-85.
https://hdl.handle.net/21.15107/rcub_cherry_1074

Milutinovic M, Sallard S, Manojlović DD, Mano N, Šojić N. Electrogenerated chemiluminescence in an electrodeposited redox polymer. in Luminescence. 2010;25(2):85-85.
https://hdl.handle.net/21.15107/rcub_cherry_1074 .

Milutinovic, Milena, Sallard, Sebastien, Manojlović, Dragan D., Mano, Nicolas, Šojić, Nešo, "Electrogenerated chemiluminescence in an electrodeposited redox polymer" in Luminescence, 25, no. 2 (2010):85-85,
https://hdl.handle.net/21.15107/rcub_cherry_1074 .