TY  - CONF
AU  - Petrović, D.
AU  - Kovačević, Gordana
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Strodel, B.
AU  - Prodanović, Radivoje
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1978
PB  - Wiley-Blackwell, Hoboken
C3  - FEBS Journal / Federation of European of Biochemical Societies
T1  - Preventing oxidative damage at the early phase: The case of glucose oxidase
VL  - 282
SP  - 339
EP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1978
ER  - 

@conference{
author = "Petrović, D. and Kovačević, Gordana and Ostafe, Raluca and Fischer, Rainer and Strodel, B. and Prodanović, Radivoje",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "FEBS Journal / Federation of European of Biochemical Societies",
title = "Preventing oxidative damage at the early phase: The case of glucose oxidase",
volume = "282",
pages = "339-340",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1978"
}

Petrović, D., Kovačević, G., Ostafe, R., Fischer, R., Strodel, B.,& Prodanović, R.. (2015). Preventing oxidative damage at the early phase: The case of glucose oxidase. in FEBS Journal / Federation of European of Biochemical Societies
Wiley-Blackwell, Hoboken., 282, 339-340.
https://hdl.handle.net/21.15107/rcub_cherry_1978

Petrović D, Kovačević G, Ostafe R, Fischer R, Strodel B, Prodanović R. Preventing oxidative damage at the early phase: The case of glucose oxidase. in FEBS Journal / Federation of European of Biochemical Societies. 2015;282:339-340.
https://hdl.handle.net/21.15107/rcub_cherry_1978 .

Petrović, D., Kovačević, Gordana, Ostafe, Raluca, Fischer, Rainer, Strodel, B., Prodanović, Radivoje, "Preventing oxidative damage at the early phase: The case of glucose oxidase" in FEBS Journal / Federation of European of Biochemical Societies, 282 (2015):339-340,
https://hdl.handle.net/21.15107/rcub_cherry_1978 .

TY  - JOUR
AU  - Perovic, I.
AU  - Milovanovic, M.
AU  - Stanić, Dragana
AU  - Burazer, Lidija M.
AU  - Petrović, D.
AU  - Milčić-Matić, Natalija
AU  - Gafvelin, Guro
AU  - van Hage, Marianne
AU  - Jankov, Ratko M.
AU  - Ćirković-Veličković, Tanja
PY  - 2009
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/600
AB  - Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446.
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - Clinical and Experimental Allergy
T1  - Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation
VL  - 39
IS  - 3
SP  - 435
EP  - 446
DO  - 10.1111/j.1365-2222.2008.03158.x
ER  - 

@article{
author = "Perovic, I. and Milovanovic, M. and Stanić, Dragana and Burazer, Lidija M. and Petrović, D. and Milčić-Matić, Natalija and Gafvelin, Guro and van Hage, Marianne and Jankov, Ratko M. and Ćirković-Veličković, Tanja",
year = "2009",
abstract = "Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446.",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Clinical and Experimental Allergy",
title = "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation",
volume = "39",
number = "3",
pages = "435-446",
doi = "10.1111/j.1365-2222.2008.03158.x"
}

Perovic, I., Milovanovic, M., Stanić, D., Burazer, L. M., Petrović, D., Milčić-Matić, N., Gafvelin, G., van Hage, M., Jankov, R. M.,& Ćirković-Veličković, T.. (2009). Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Clinical and Experimental Allergy
Wiley-Blackwell Publishing, Inc, Malden., 39(3), 435-446.
https://doi.org/10.1111/j.1365-2222.2008.03158.x

Perovic I, Milovanovic M, Stanić D, Burazer LM, Petrović D, Milčić-Matić N, Gafvelin G, van Hage M, Jankov RM, Ćirković-Veličković T. Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Clinical and Experimental Allergy. 2009;39(3):435-446.
doi:10.1111/j.1365-2222.2008.03158.x .

Perovic, I., Milovanovic, M., Stanić, Dragana, Burazer, Lidija M., Petrović, D., Milčić-Matić, Natalija, Gafvelin, Guro, van Hage, Marianne, Jankov, Ratko M., Ćirković-Veličković, Tanja, "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation" in Clinical and Experimental Allergy, 39, no. 3 (2009):435-446,
https://doi.org/10.1111/j.1365-2222.2008.03158.x . .

TY  - CONF
AU  - Ćirković-Veličković, Tanja
AU  - Perovic, I.
AU  - Petrović, D.
AU  - Gavrović-Jankulović, Marija
AU  - Burazer, Lidija M.
AU  - Milovanovic, M.
AU  - Atanasković-Marković, Marina
AU  - Jankov, Ratko M.
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/768
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation
VL  - 62
SP  - 260
EP  - 260
UR  - https://hdl.handle.net/21.15107/rcub_cherry_768
ER  - 

@conference{
author = "Ćirković-Veličković, Tanja and Perovic, I. and Petrović, D. and Gavrović-Jankulović, Marija and Burazer, Lidija M. and Milovanovic, M. and Atanasković-Marković, Marina and Jankov, Ratko M.",
year = "2007",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation",
volume = "62",
pages = "260-260",
url = "https://hdl.handle.net/21.15107/rcub_cherry_768"
}

Ćirković-Veličković, T., Perovic, I., Petrović, D., Gavrović-Jankulović, M., Burazer, L. M., Milovanovic, M., Atanasković-Marković, M.,& Jankov, R. M.. (2007). Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Allergy
Blackwell Publishing, Oxford., 62, 260-260.
https://hdl.handle.net/21.15107/rcub_cherry_768

Ćirković-Veličković T, Perovic I, Petrović D, Gavrović-Jankulović M, Burazer LM, Milovanovic M, Atanasković-Marković M, Jankov RM. Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Allergy. 2007;62:260-260.
https://hdl.handle.net/21.15107/rcub_cherry_768 .

Ćirković-Veličković, Tanja, Perovic, I., Petrović, D., Gavrović-Jankulović, Marija, Burazer, Lidija M., Milovanovic, M., Atanasković-Marković, Marina, Jankov, Ratko M., "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation" in Allergy, 62 (2007):260-260,
https://hdl.handle.net/21.15107/rcub_cherry_768 .